Method for simultaneously detecting 48 stimulants in animal body fluid

Abstract

The invention relates to a method for simultaneously detecting 48 stimulants in animal body fluid, which comprises the following steps: extracting a sample by using an acetonitrile solution of formic acid, removing water by using anhydrous sodium sulfate, blow-drying the obtained supernatant, dissolving residues by using an aqueous solution of acetonitrile, carrying out vortex oscillation filtration to form a solution to be detected, determining the stimulant in the to-be-detected solution and the mixed standard working solution by using a high performance liquid chromatography tandem mass spectrometer, comparing the obtained spectrogram information, and judging that a corresponding target object exists in the sample; according to the peak areas of the stimulant and the corresponding internal standard substance, combining the extraction volume, the sample amount and the dilution ratio of each to-be-detected solution in the acquisition process, and determining the contents of zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an external standard method for quantification, anddetermining the content of all stimulants except for zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an internal standard method for quantification.

Description

technical field [0001] The invention relates to the technical field of stimulant detection, in particular to a method for simultaneously detecting forty-eight stimulants in animal body fluids. Background technique [0002] In large-scale sports events, the urine test of athletes is mainly to detect anabolic agents, β2-agonists, glucocorticoids, diuretics and other stimulants in urine. Because the products of metabolism in the human body are mainly detoxified by the kidneys and then excreted with urine. As the nutritional supply for athletes, all kinds of livestock and poultry meat must be ingested, and an important way to monitor whether livestock and poultry meat contains stimulants is to monitor the content of stimulants in animal body fluids. Compared with other body tissues, animal body fluid has the advantages of pre-mortem detection and daily monitoring. Therefore, testing the content of stimulants in animal body fluids can effectively monitor the content of stimulan...

AI Technical Summary

Problems solved by technology

In order to effectively solve the problems of derivatization, complicated operation, long detection cycle, and poor sensitivity in the detection process of certain doping agents, more advanced and more sensitive gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry have been adopted. However, there is no relevant report on the detection methods of 48 common stimulants, such as β2-agonist stimulants, anabolic agent stimulants, glucocorticoid stimulants and diuretic stimulants.

[0004] Therefore, at present, there are no relevant reports on the pretreatment of animal body fluids and the detection of 48 common stimulants, which has caused the problem that the 48 common stimulants in animal body fluids cannot be detected at the same time

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