Method for detecting seven protein assimilation preparation type stimulants in animal feed

Abstract

The invention provides a method for detecting seven protein assimilation preparation stimulants in animal feed, which comprises the following steps of: adding an isotope internal standard intermediate solution, deionized water and dichloromethane into a sample for extraction, purifying a dichloromethane layer, blowing the obtained supernatant to be nearly dry, dissolving by using a mixed solution of acetonitrile, formic acid and water, carrying out vortex oscillation, filtering to obtain a to-be-detected solution, and determining by using a high performance liquid chromatography tandem mass spectrometer to obtain corresponding spectrogram information; determining the mixed standard working solution under the same condition, and comparing two groups of spectrogram information; and finally, selecting corresponding parameters from the content and the peak area of a to-be-detected component in the mixed standard working solution, the concentration and the peak area of an internal standard substance in the to-be-detected solution, the peak area of the to-be-detected component in the to-be-detected solution, the peak area and the concentration of an internal standard substance in the mixed standard working solution, the extraction volume, the sample weight, the extraction volume and the constant volume of a sample solution and the dilution ratio, and calculating the content of all stimulants by using an internal standard method and an external standard method.

Description

technical field [0001] The invention relates to the technical field of stimulant detection, in particular to a method for detecting 7 kinds of anabolic stimulants in animal feed. Background technique [0002] Doping detection is the most important supervision link in the competition process of the sports meeting. After animals eat stimulant-containing feed, it will cause different degrees of residues in muscles and tissues, and then remain in the human body through the biological chain, which may cause different degrees of poisoning symptoms in the eaters, and athletes are also among the eaters . In order to maintain the fairness and purity of competitive sports and ensure the health of athletes, doping testing has become an indispensable means in all sports competitions. [0003] Plasterone (dehydroepiandrosterone) is a common steroid hormone in the peripheral circulation of the body, which will be converted into a steroid drug in the blood, which contains testosterone, w...

AI Technical Summary

Problems solved by technology

Previously, high-performance liquid chromatography (HPLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC / MSD) and other methods were used for detection, but the detection of these 7 anabolic agents required Hydrolysis, purification, etc. will cause problems such as complex operation, long detection cycle, and poor sensitivity. Many scientific and technological workers have adopted more advanced and more sensitive gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry detection methods. However, the detection of these 7 anabolic stimulants is still not possible

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