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A detection method, a doping technology, applied in the direction of measuring devices, material separation, analysis of materials, etc.
Pending Publication Date: 2022-01-25
西安市食品药品检验所西安市药品不良反应监测中心
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[0005] Aiming at the problems existing in the prior art, the present invention provides a method for detecting 7 kinds of stimulants in spices, which solves the problem that the 7 kinds of stimulants in spices cannot be simultaneously measured at present
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[0053] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.
[0054] A kind of method that the present invention detects these 7 kinds of stimulants of tritoquinol, higenamine, papaverine, codeine, narcotine, morphine and thebaine in spices, comprises the following steps:
[0055] Step 1) preparation of standard solution
[0056] 1. Standard stock solution:
[0057] a) Higenamine standard stock solution: Accurately weigh an appropriate amount of higenamine standard substance (accurate to 0.01mg), dissolve it in methanol, prepare a 1mg / mL standard stock solution, and store it below -18°C in the dark Store frozen.
[0058] b) Tratoquinol standard stock solution: Accurately weigh an appropriate amount of Tratoquinol hydrochloride standard substance (accurate to 0.01 mg), dissolve it in acetonitrile, prepare a 1 mg / mL standard stock solution, and freeze i...
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Abstract
The invention relates to a method for detecting seven stimulants in spices. The method comprises the following steps of: adding an isotope internal standard intermediate solution into a sample, extracting by using deionized water and an acetonitrile solution of formic acid, separating out supernate, combining the rest extracts, adding anhydrous magnesium sulfate, carrying out vortex oscillation and centrifugation, blowing the obtained supernate to be nearly dry, and performing dissolving and filtering; measuring the to-be-measured solution and the mixed standard working solution by using a high performance liquid chromatography tandem mass spectrometer to obtain spectrogram information; calculating the contents of higenamine, tratoquinol, papaverine, narcotine and thebaine by combining the content and the peak area of a component to be detected in the mixed standard working solution and the peak area, the sample mass, the extraction volume and the dilution ratio of the component to be detected in the solution to be detected; and calculating the content of the residual stimulant by combining the content and the peak area of the component to be detected in the mixed standard working solution, the peak area and the concentration of the internal standard substance, the concentration and the peak area of the internal standard substance in the solution to be detected, the peak area of the component to be detected in the solution to be detected, the mass of the sample to be detected, the extraction volume and the dilution ratio.
Description
technical field [0001] The invention belongs to the technical field of stimulant detection in food, and specifically relates to a method for detecting 7 kinds of stimulants in spices. Background technique [0002] In order to maintain the fairness and purity of competitive sports and ensure the health of athletes, doping testing has become an indispensable part of all sports competitions. Spices have a pungent aroma, and use plant seeds, flower buds, leaf stems, roots, etc., or their extracts, to impart flavor to food, increase appetite, help digestion and absorption, and will be used in cooking and Chinese patent medicines. Athletes may misuse food and medicine by mistake. [0003] The spices contain 7 common stimulants, namely, tratoquinol, higenamine, papaverine, codeine, narcotin, morphine, and thebaine. There are risks of papaverine, codeine, narcotin, morphine, and thebaine in spices, seasonings, and hot pot bottoms; tritoquinol is a plant-derived ingredient that has...
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