The invention relates to a nanometer selenium probiotics composite leaf surface nutrient solution and application thereof in the production of selenium-enriched pears. A using method of the nanometer selenium probiotics composite leaf surface nutrient solution is characterized by comprising the following steps of: uniformly mixing and dissolving a nanometer selenium water solution with the concentration of 20 g/kg, L-lysine hydrochloride, sodium glutamate, humic acid, monopotassium phosphate, trace elements, brown sugar and EM (Effective Microorganisms) original liquid probiotic bacteria according to weight ratio; then diluting according to the dilution ratio of 1:(20-50) by adding water; and spraying on leaf surfaces for 3-4 times according to different growth periods of pear trees so asto obtain selenium-enriched Nanguo pears, selenium-enriched ussurian pears and selenium-enriched sharp stalk pears. Compared with the prior art, the invention not only enables the pears to be rich inselenium, but also increases the yield and enhances the quality, has integrated benefit, bright and smooth pericarp and fine and tasty sarcocarp, increases the saccharinity by 0.5-1.3 percent and theyield by 5-10 percent, prolongs the storage period by about 30 days and has the selenium content of 0.010-0.050 mg/kg, wherein the selenium content is 2-10 times more than that of a control group; and in addition, by adopting the invention, the selenium-enriched apples, the selenium-enriched peaches, the selenium-enriched grapes, the selenium-enriched cherries and the selenium-enriched strawberries can be produced.