Rotary disc-type microfluidic concentration measuring apparatus and method based on luminosity detection

Abstract

The invention discloses a rotary disc-type microfluidic concentration measuring apparatus and method based on luminosity detection. A rotary disc-type microfluidic chip, a luminosity detection device support and an AC servo motor are arranged in a hermetic darkroom; an output shaft motor shaft of the AC servo motor is vertically upward and is coaxially equipped with the horizontal rotary disc-type microfluidic chip; and N stages of test solution interinfiltration bands are arranged on the rotary disc-type microfluidic chip, each stage of test solution interinfiltration band is in the shape of a circular arc with the center of the rotary disc-type microfluidic chip as the center of circle, and two adjacent stages of test solution interinfiltration bands are connected through a spring-like test solution channel and communicated with each other. According to the invention, effective positioning of dilution concentration is realized through combined usage of the rotary disc-type microfluidic chip and rotary dilution positioning technology, so a dilution ratio is rapidly calculated; the accurate concentration of a to-be-measured solution is backwardly deduced by multiplying the dilution ratio by corresponding relationship between known optical length and absorbance produced at optimal concentration; and sample introduction, detection and concentration calculation are automatically carried out.

Description

technical field [0001] The invention relates to microfluidic photometric detection technology, in particular to a microfluidic mixed solution concentration measurement device and method based on photometric detection. Background technique [0002] Microfluidic photometric detection technology is currently the most widely applicable microfluidic detection method. However, the photometric detection cell of the microfluidic chip is often fixed, and its optical path cannot be changed arbitrarily according to the concentration of the liquid to be detected. But according to the spectrophotometric error theory, the best absorbance in the measurement A OPT is 1 / ln10, and the corresponding optimal optical path length is 0.434 αC 0 ( C 0 is the concentration of absorbate in the solution, α is the absorbance coefficient of the absorber). Therefore, only when the concentration is compatible with the detection optical path, the photometric detection noise is the smallest, and the ...

AI Technical Summary

Problems solved by technology

However, in the actual detection process, the concentration value of the substance to be detected is often not known in advance, so it is difficult to judge in advance the selection of the optimal optical path value

In addition, this device is only suitable for photometric detection on a traditional scale, and is often not applicable to microfluidic photometric detection systems that require a higher optical path

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