The invention relates to an exposure time determination-based laser speckle blood flow imaging method, which comprises the following steps of building a laser speckle blood flow imaging system, then, inputting the imaging of a detected object into a computer by a charge coupled device (CCD) camera according to exposure time T, and selecting a target detection point; continuously collecting n frame images from the target detection point by the CCD camera respectively according to the exposure time T1, T2,?-, Tm to obtain m image sets P<T1>(n), P<T2>(n),?- P<Tm>(n), and inputting into the computer for processing; calculating the image gray value contrast K(x, y, Ti) under different exposure time Ti by the computer according to a formula; obtaining a fitted value A<fit> of a reciprocal A of Tao<c> by calculating and fitting a relation between the square of the contrast K2(x, y, Ti) and the exposure time Ti of the CCD camera and the self-correlation function fall time of scattering light-intensity variation Tao<c> according to the laser speckle blood flow imaging theory; and setting the exposure time T0=1.1/A<fit> and performing blood flow detection on the detected object to obtain blood flow velocity distribution. According to the exposure time determination-based laser speckle blood flow imaging method provided by the invention, according to the blood flow velocity of different detected objects, the exposure time of the CCD camera is automatically determined, and the accuracy of the laser speckle blood flow imaging method monitoring blood flow variation is improved.