Microfluidic chip and single cell protein quantitative detection device and method

Abstract

The invention provides a microfluidic chip and a single cell protein quantitative detection device and method. The chip is composed of a PDMS (Polydimethylsiloxane) layer and a quartz layer; a microfluidic chip technology is combined with a liquid drop formation technology, a fluorescence antibody preparation technology and a fluorescence detection technology and then high-throughput quantitative detection of proteins in single cells is realized; reliable device and method are provided for characterization of cell biological characteristics. By designing and manufacturing a fluorescence antibody pair and setting an optical filter, the detection precision can be effectively improved and detection errors are reduced. Equipment including an inverted fluorescent microscope, a photomultiplier and the like can be used in a traditional biological laboratory, so that the device and the method have strong adaptability and high portability and are convenient to realize.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a microfluidic chip, a single-cell protein quantitative detection device and a method. Background technique [0002] As the main component of living organisms, protein dominates or participates in almost all biological activities and functions. The quantity and activity of proteins are the most important indicators reflecting changes in life activities, and are closely related to cell differentiation and nerve conduction. Therefore, a variety of protein analysis methods, such as gel electrophoresis, immunoassay, chromatography and mass spectrometry, have been developed and applied to the study of the molecular mechanism of cellular processes, vaccines and drugs. However, these methods are developed for a large number of macroscopic proteins, which require a sufficient number of cells to analyze, and the final result is the average result at the level of the cell population, wh...

AI Technical Summary

Problems solved by technology

[0007] The calibration microspheres used in flow cytometry can only quantify cell membrane proteins, and the method of quantitatively modifying fluorescent molecules inside the calibration microspheres is not yet mature, and the traditional calibration microsphere method cannot be used to quantify protein expression in a single cell detection

[0008] Existing single-cell protein detection methods based on microfluidic technology have problems such as low throughput and inability to detect cytoplasmic proteins

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