154 results about "Cell protein" patented technology

The invention belongs to the technical field of biological detection, and discloses a kit and a method for quantitatively detecting residual quantity of host cell protein of pichia pastoris in order to solve the problems of limit of quantitation and low detection sensitivity of an existing detection method. According to the present invention, the empty plasmid without the recombinant human serum albumin gene is introduced into the pichia pastoris, and purification is performed by combining the Protein A chromatographic column and the host cell protein coupling affinity column, such that the prepared polyclonal antibody can be effectively used for the quantitative detection of the residual amount of the pichia pastoris host cell protein in the biological product; the kit formed by the polyclonal antibody has good accuracy and repeatability on the quantitative detection of the host cell protein residues in the pichia pastoris source biological products, the quantification limit is 0.2 ng / mL, and the kit provided by the invention can meet the quantitative detection of the host cell protein residues in all the pichia pastoris source biological products.

The invention provides a cell protein degradation platform based on an artificial biological macromolecular aggregate. Specifically, the invention provides a PROTAC functional module based on an interworking nucleic acid skeleton, a PROTAC-aggregate complex (MLO-PROTAC), a kit, application and a preparation method of the PROTAC functional module, the PROTAC-aggregate complex (MLO-PROTAC) and the kit, and also provides a targeted protein degradation method. In a PROTAC platform built by the PROTAC functional module and the PROTAC-aggregate complex, a programmable nucleic acid component is used as a core assembly unit, and the functional module is enriched and spatiotemporal-spatial regulation is performed by using the polypeptide aggregate, so that the delivery efficiency and the cytoplasm exposure degree are remarkably improved while the universality is maintained, and the delivery efficiency and the cytoplasm exposure degree are remarkably improved. Therefore, a more effective target protein degradation way is provided for the field.

The invention discloses kluyveromyces marxianus and application of the kluyveromyces marxianus in production of single-cell protein by taking straw as a raw material. The Kluyveromyces marxianus is a ZH-2 bacterial strain which is CGMCC (China General Microbiological Culture Collection Center) No.32295 The strain is obtained from a wild strain in a laboratory adaptive evolution mode, wheat straw and an inorganic nitrogen source can be used as raw materials for efficiently producing single-cell protein, compared with a parent strain, the utilization speed and the utilization rate of biomass, glucose and xylose are remarkably increased, the yield of ZH-2 SCP can reach 0.047 g.g <-1 >, and the yield of ZH-2 SCP can reach 0.045 g.g <-1 >. And the essential amino acid mode basically accords with adult and infant amino acid modes, and can be used as high-quality protein nutrition supply. The Kluyveromyces marxianus ZH-2 strain capable of obtaining single-cell protein by fermenting wheat straw as a raw material is provided from the perspective of utilizing cheap waste resources, so that the production cost of SCP is greatly reduced, and the problem of protein resource shortage is effectively solved.

The present disclosure provides materials and methods related to the purification of viral vectors. In particular, the present disclosure provides peptides, compositions, adsorbents, and related methods, capable of removing process-related impurities (e.g., host cell proteins, nucleic acids, and media components) and product-related impurities (e.g., product fragments, product aggregates, and inactive forms derived from product degradation by or association with other species in the cell culture harvest) from samples during the production and purification of adenovirus.

The current invention reports a method for purifying an antibody by reducing the content of a host cell protein. The method employs a wash step with a low conductivity aqueous solution in an affinity chromatography.

The disclosure relates to methods of detecting Host Cell Proteins (HCPs) in biopharmaceutical samples containing a drug product. More specifically, the disclosure relates to methods of assaying HCPs in biopharmaceutical samples using enzymatic digestion protocols, liquid chromatography (LC) gradients, immunoaffinity capture protocols, and High-Resolution Mass Spectrometry (HRMS) to enhance the recovery, detection, and profiling of HCPs, which can impact the efficacy, shelf-life, and therapeutic response of drug products.

The invention relates to the technical field of bioengineering, in particular to a preparation method and application of an antibacterial peptide, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID No: 1 and is named as L8. The preparation method of the antibacterial peptide L8 comprises the following steps: S1, obtaining an L8 target gene segment; connecting an L8 target gene segment with a pLC vector to obtain a connection product; transforming the connection product into an escherichia coli DH5-alpha competent cell, and screening positive clones to obtain a pLC-L8 recombinant plasmid; s2, taking the pLC-L8 recombinant plasmid as a substrate, and carrying out cell-free protein synthesis reaction to obtain the antibacterial peptide L8, the cell-free protein synthesis system comprises 175 mM of potassium glutamate, 10 mM of ammonium glutamate, 2.7 mM of potassium oxalate, 33 mM of PEP, 38 mM of compound amino acid, 10 mM of magnesium glutamate, 2% (w / v) PEG-8000, 5 nM of pLC-L8 recombinant plasmid, 11.25 mM of lactose, 33.33% (v / v) of crude extract, 100 mg / mL of escherichia coli tRNA and 2.5 nM of T7 RNA polymerase. The antibacterial peptide L8 is obtained by optimizing the antibacterial peptide LRGG, and compared with the LRGG, the antibacterial peptide L8 subjected to cell-free protein synthesis reaction expression has better antibacterial activity and biological safety.

The invention provides a recombinant herpes simplex virus and a herpes simplex virus modification method. The herpes simplex virus modification method comprises the step of replacing an ICP4 protein coding gene in a herpes simplex virus genome containing an infected cell protein 4 (ICP4) gene with an hTERTp-fluorescent protein expression cassette. The expression cassette comprises an hTERTp promoter and a fluorescent protein coding sequence controlled by the hTERTp promoter, and the transcription direction is opposite to that of an ICP4 promoter in a genome. The hTERTp-fluorescent protein expression cassette in the recombinant virus obtained by the method disclosed by the invention can be normally expressed in response to telomerase activity. Therefore, the recombinant virus is capable of expressing a fluorescent protein, such as mBaoJin, in a cell having human telomerase activity. Cells infected by the virus can be identified through fluorescence signals, and the higher the telomerase activity is, the stronger the fluorescence intensity is. The virus has wide application value in research of tumor action mechanisms and stem cell action mechanisms, health assessment, screening of tumor drugs, research and development of diagnostic reagents and establishment of animal models.

The present disclosure provides materials and methods related to the purification of a biologic from a biological fluid. In particular, the present disclosure provides compositions, and related methods, comprising peptide ligands capable of removing process-related impurities (e.g., host cell proteins, nucleic acids, and media components) and product-related impurities (e.g., product fragments, product aggregates, and inactive forms derived from product degradation by or association with other species in the cell culture harvest) from biological fluids during the production of a biologic.

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

The invention discloses candida utilis engineering bacteria and a method for producing single-cell protein by fermenting corn thin pulp, and belongs to the technical field of biology. According to the candida utilis engineering bacteria disclosed by the invention, the candida utilis is subjected to gene modification, a chitin synthase gene in the candida utilis is knocked out, and an exogenous keratinase gene is integrated, so that the candida utilis engineering bacteria can utilize corn thin pulp as a substrate for fermentation. In the fermentation process, carbon and nitrogen sources in the thin corn pulp can be effectively utilized for growth, macromolecular protein in the thin corn pulp can be hydrolyzed and converted into single-cell protein, the content of the single-cell protein in final fermentation liquor is increased, the protein content of the final fermentation liquor can reach 60% or above, and the proportion of the single-cell protein can reach 30% or above; the corn thin pulp can be efficiently utilized, and meanwhile, single-cell protein in the fermentation liquor can be used for adding animal feed so as to meet the requirement for animal protein feed in the market.

The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and / or hydrolases.

The invention relates to the technical field of microbial fermentation, and provides a method for producing single-cell protein, which comprises the following steps: inoculating a crop straw matrix with a first microbial seed for first fermentation; the first microbial seed comprises trametes versicolor; sterilizing a fermentation product obtained by the first fermentation; inoculating second microbial seeds into the sterilized fermentation product for second fermentation; the second microbial seed comprises yarrowia lipolytica / candida utilis. According to the scheme, the combination of the selected first microbial seed solution and the second microbial seed solution has a remarkable synergistic effect, so that the crude protein content, the lignin removal rate, the cellulose retention rate and the yeast biomass are improved, and the hemicellulose loss rate is reduced.

The object of the present invention is to provide a protein production method capable of producing an active protein with high efficiency even at a low temperature, and a cell-free protein synthesis kit. A protein production method including producing a protein with a reaction solution of a cell-free protein synthesis system containing either one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein. A cell-free protein synthesis kit including one or both of a cold shock protein and a nucleic acid containing a coding region encoding an amino acid sequence of the cold shock protein, and a reaction solution of a cell-free protein synthesis system.

The present disclosure provides materials and methods related to the purification of viral vectors. In particular, the present disclosure provides compositions, and related methods, comprising peptide ligands capable of removing process-related impurities (e.g., host cell proteins, nucleic acids, and media components) and product-related impurities (e.g., product fragments, product aggregates, and inactive forms derived from product degradation by or association with other species in the cell culture harvest) from biological fluids during the production and purification of adeno-associated viruses (AAVs).

A Pichia pastoris strain and its application in the fermentation production of single-cell protein belong to the field of microbial fermentation technology. The Pichia pastoris strain... Komagataella phaffii GG7 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 39081, on December 2, 2025. This Pichia pastoris strain GG7 was isolated from soil in a methanol production plant area. It maintains good growth even in high concentrations of methanol (up to 8% (w / v)) and mixed carbon sources such as methanol, acetic acid, and ethanol, achieving a single-cell protein content of approximately 62 wt%. This strain is suitable for aerobic fermentation using methanol, ethanol, and / or acetic acid as carbon sources, exhibiting strong carbon source tolerance and high protein yield, making it suitable for industrial production of single-cell protein.

The application discloses a kind of bionic transmembrane protein affinity chromatography column and its preparation method and application.The system (iSTAC) realizes the in-situ construction of transmembrane protein in highly bionic dynamic microenvironment by integrating cell-free protein synthesis, functional mesoporous silica modification and amphiphilic (AH) peptide stabilized planar lipid bilayer technology.The core is to use long-chain PEG-24 crosslinking agent to retain lipid bilayer on the surface of silica gel, provide sufficient conformational dynamic space for multi-transmembrane protein, and introduce AH peptide to repair membrane defects, ensure that the receptor realizes directional embedding while maintaining natural functional attributes.The preparation cycle is shortened from 168 hours to 5 hours, which significantly improves the efficiency of targeted drug screening and in-situ analysis of binding sites.Using this platform, 5-HT 1A Receptor agonists crocin I and crocin II with anti-insomnia and neuroprotective effects are successfully screened from saffron, and the action site is accurately depicted.

The present disclosure relates to a purification method of a monoclonal antibody. The purification method of the present disclosure adopts two-step chromatography to purify a monoclonal antibody against human CCR8, has fewer purification steps, high sample recovery rate, stable process, linear amplification, can obtain a monoclonal antibody with high purity, has less residual host cell protein, DNA, etc. in the purified product, and the purified monoclonal antibody has relatively high biological activity and biosafety.

The invention provides an application of teconazole (C26H31Cl2N5O3) or a pharmaceutical salt or a derivative thereof in a medicine for treating age-related retinal diseases, and relates to the technical field of biology. The application of the invention comprises the application of the teconazole or the pharmaceutical salt or the derivative thereof to the preparation of the medicine for treating, relieving and / or preventing the age-related retinal diseases. The invention proves that the Terconazole can relieve the senescence of retinal pigment epithelial cells and reduce the p53 protein content of the cells, provides a new candidate drug for the treatment of age-related retinal diseases, and has remarkable medical and clinical values.

The present invention relates to an improved method for culturing microorganisms capable of utilizing ethanol as a starting material using continuous culture. The present invention relates to an improved process for the production of biomass, in particular single cell proteins, using continuous culture, wherein the yeast single cell protein product comprises yeast cells of the Saccharomyces cerevisiae; and an animal feed comprising such biomass.

The present application belongs to the field of environmental biotechnology and resource recycling technology, and provides a high-ammonia-nitrogen-tolerant yeast strain and its application in biogas slurry resource treatment. The high-ammonia-nitrogen-tolerant yeast strain is named Torulaspora delbrueckii L12345, which was preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 13, 2026, and the address is No. 1, Yitian West Road, Chaoyang District, Beijing, China Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No. 39123. The present application realizes efficient treatment of biogas slurry and simultaneous production of single-cell protein, reduces the treatment cost, improves the economic benefit, and provides a new technology for clean and high-value utilization of biogas slurry.

The present application relates to the technical field of animal feed, more particularly, to a methanol-based single-cell protein feed composition and a preparation method thereof.The feed composition comprises the following raw material components by weight: 30-70 parts of methanol-based single-cell protein powder, 10-40 parts of plant protein source, 10-30 parts of energy source, 1-10 parts of functional additive, and 5-20 parts of carrier.The present application uses the methanol-based single-cell protein, a non-grain protein source with high protein and good amino acid composition, to significantly improve the palatability, digestibility and nutritional value of the feed by compounding the methanol-based single-cell protein with conventional feed components through a scientific formula and a physical wall-breaking and mixing process, and to partially or completely replace traditional protein sources such as soybean meal, thereby reducing the cost of feed, alleviating the problem of food competition between humans and animals, and providing a sustainable new protein feed solution for the animal husbandry.

The invention discloses a biological pretreatment method for improving the true protein production efficiency of wet distiller's grains, and belongs to the technical field of biological pretreatment, the method comprises the following steps: by combining chemical and biological multi-stage pretreatment modes, alkali and urea are combined in the first-stage chemical treatment process, and the second-stage chemical treatment process is combined in the second-stage chemical treatment process; the fiber structure of the wet distiller's grains is better damaged at normal temperature, and infectious microbe pollution is reduced for subsequent fermentation. In the second-stage anaerobic fermentation treatment, fibers in the wet distillers' grains are converted into sugar through the synergistic effect of bacteria and enzymes, the pH of the wet distillers' grains is reduced, and conditions are provided for growth of subsequent aerobic fermentation candida utilis yeast thalli. In the third stage of aerobic fermentation treatment of the candida utilis, urea and fiber in the pretreatment process in the early stage are used for degrading sugar to generate mycoprotein, so that the true protein improvement rate and the fiber degradation rate of the white spirit vinasse after pretreatment are effectively improved, the loss rate in the pretreatment process is greatly reduced, and finally, the true protein production efficiency of the wet white spirit vinasse is improved.

The application discloses a single-cell immunoblotting method based on quenchable aptamer probes and relates to the technical field of single-cell protein detection. The method comprises the following steps: S1, a single-cell suspension sample is settled in a microwell array on a composite hydrogel chip; S2, the composite hydrogel chip is placed in an electrophoresis tank, then a lysis solution is slowly poured into the tank, and incubation lysis is carried out; S3, after cell lysis, an electric field is applied to both ends of the chip, and proteins enter a gel coating layer on the surface of the chip under the action of the electric field and start electrophoretic separation; S4, after gel electrophoresis is completed, the chip is placed under an ultraviolet lamp for exposure, and the gel is taken out after exposure is completed; S5, the gel is placed in an aptamer probe solution which is targeted to target proteins, and is soaked and dyed, and the fluorescence signal intensity of the target protein molecules is measured under a laser confocal fluorescence microscope. The application can improve the detection sensitivity of target proteins and has fast imaging speed.

The invention belongs to the technical field of synthetic biology and fermentation engineering, and particularly relates to a coupling fermentation method for synthesizing single-cell protein from methanol and carbon dioxide. The coupling fermentation method comprises the following steps: mixing and stirring a fermentation medium and methanol according to a weight part ratio of (97-99): (1-3) to form a fermentation substrate mixed solution; mixing the pre-cultured saccharomycetes liquid, the pre-cultured photosynthetic bacteria liquid and the fermentation substrate mixed liquid according to a weight part ratio of (2-4): (3-6): (90-95), introducing a mixed gas source, and carrying out illumination fermentation to obtain fermentation liquid; and sequentially carrying out centrifugal separation and vacuum drying on the fermentation liquor to obtain single-cell protein. According to the method, the pre-cultured yeast liquid, the pre-cultured photosynthetic bacterium liquid and the fermentation substrate mixed liquid are mixed and then are subjected to aerated fermentation, so that the fermentation capacity of microorganisms at a relatively high fermentation temperature is improved, and the yield of single-cell protein is increased.

This invention provides a method for producing single-cell protein using a mixed bacterial culture, comprising the following steps: performing primary seed culture on methanogenic bacteria, *Tricholoma materata*, and *Glucosinolate methyl-eating bacteria* to obtain primary seed solutions; mixing the primary seed culture solutions evenly to obtain a mixed bacterial solution; inoculating the obtained mixed bacterial solution into a secondary seed culture medium for cultivation to obtain a secondary seed solution; inoculating the obtained secondary seed solution into a fermentation medium for scale-up cultivation to obtain a fermentation broth; separating the solid and liquid components of the obtained fermentation broth, harvesting the bacterial cells, and obtaining single-cell protein. This method for producing single-cell protein using a mixed bacterial culture improves the utilization rate of the carbon source methane and the yield of single-cell protein.

The invention discloses an eluent for improving the anion exchange chromatography recovery rate of lentivirus and application of the eluent. The eluent is prepared from a basic buffer solution, 50 to 500 mM of NaCl and 0.2 to 2 M of L-arginine; the pH (Potential of Hydrogen) value of the eluent is 6.5 to 8.0. In order to solve the problems that in the prior art, high-salt elution is adopted, so that the lentivirus activity damage is large, and the recovery rate is low, the concentration of a main elution component NaCl in an eluent is reduced, then the main elution component NaCl is compounded with L-arginine, the lentivirus can be effectively eluted, meanwhile, the damage of a high-salt environment to a virus envelope structure is avoided, and the yield of the lentivirus is improved. And the elution efficiency is improved by utilizing the characteristic that L-arginine inhibits protein interaction. When the eluent is used for chromatographic purification, the recovery rate and activity of the lentivirus can be remarkably improved, impurities such as host cell protein and the like are effectively removed, and a key purification solution is provided for preparing a high-purity and high-activity lentiviral vector for gene therapy.

The invention belongs to the technical field of antibody purification, and relates to a method for removing impurities in a sample containing a trispecific antibody. According to the method provided by the invention, impurities, especially aggregates and fragments, can be well removed, and the purity detected by molecular exclusion chromatography-high performance liquid chromatography (SEC-HPLC) is increased from about 74% to 98% or above; the purity detected by non-reduction method lauryl sodium sulfate capillary electrophoresis (NR-CE-SDS) is improved from about 76% to 93% or above. Besides, the method provided by the invention can control the content of host cell protein at a lower level, greatly improves the quality of a tri-specific antibody product, and has the advantages of stable process and easiness in large-scale production.

The application discloses a biological pretreatment method for improving the efficiency of producing true protein from wet distiller's grains, and belongs to the technical field of biological pretreatment, which comprises the following steps: by combining chemical and biological multi-stage pretreatment methods, alkali and urea are used in the first chemical treatment process to realize better destruction of the fiber structure of the wet distiller's grains at normal temperature and reduce the contamination of miscellaneous bacteria in subsequent fermentation; the second anaerobic fermentation treatment converts the fibers in the wet distiller's grains into sugar through the synergistic effect of bacteria and enzymes, and reduces the pH of the wet distiller's grains, thereby providing conditions for the growth of Candida utilis in subsequent aerobic fermentation; and the third aerobic fermentation treatment utilizes the urea and fiber degradation sugar in the previous pretreatment process to generate cell protein, effectively improves the true protein improvement rate and fiber degradation rate of the distiller's grains after the pretreatment is completed, greatly reduces the loss rate in the pretreatment process, and finally improves the efficiency of producing true protein from the wet distiller's grains.

Methods and apparatuses are disclosed for true spectral flow cytometers that use wide band lasers to provide excitation light to cells, proteins, antibodies, and the like. Light from wide band lasers (emitting light through a continuous, wide bandwidth of wavelengths) is projected into a flow cell as a spread out spectrum to generate a spatially arranged spectrum that can excite a fluorescent response in cells passing through the flow cell to generate at least one excitation emission matrix (EEM) plot, wherein the EEM plot includes excitation wavelength, emission wavelength and fluorescence intensity.