127 results about "Cellular Morphology" patented technology

The invention provides a multifunctional aerogel material for blood component protection and a preparation method of the multifunctional aerogel material. Gellan gum, hyaluronic acid, Pullulan, Lubrajel CG andpoly(gamma-glutamic acid), derivatives or a mixture thereof are taken as a skeleton to act with a novellight-sensitive material, aerogel or a precursor for preparation of the ready-to-useaerogel material, and then multifunctional ready-to-useaerogel composite is prepared from the ready-to-useaerogel material as well as raw materials includingglucose, a novel solvent, a novel fixing agent, a buffering agent, a stabilizer, a novel preservative, a surfactant, a high-molecular compound, essential oil, an antioxidant and the likewith a three-spectral-line high-energy photocuring method by utilizing a high polymer material such as hyaluronic acid and the like as the skeleton. The material has the characteristics of being convenient to use, green, environment-friendly and the like, the whole blood component treated with the material keeps good cellular morphology, is stored for a long time, reducesbatch-to-batch difference and is an ideal additive for a whole blood controlling product and other products, and the material can also be applied to collection, storage and transfer of tissue cells as well as fields of cosmetics, food, drugs and the like.

Methods and compositions for stabilizing tissues, cells, and cell components such that desired antigenic sites, light scatter properties and cellular morphology are preserved for a useful period of time. The stabilizing solution includes glycine, lysine and formaldehyde.

The invention discloses an exfoliative cells preserving fluid which is prepared from a pH buffering agent, an osmotic pressure maintenance agent, preservatives, a fixing agent for maintaining cellular morphology, an anticoagulant, a mucus softener, an antimicrobial reagent, a cleaning agent, a humectant and red blood cell destroying components. The components in the preserving fluid are reasonable in proportioning, and the exfoliative cells can be preserved at a long time under normal temperature, wherein the longest time can reach 2 years; mucus can be sufficiently dissolved and the red cells can be partially destroyed; a film production effect is good, cellular distribution is very even, cellular morphology is perfect, cytoplasm and cell nucleus demarcation is distinct, gradation is clear, and cytoplasm and cell nucleus transparency is very good, and the exfoliative cells preserving fluid can be simultaneously used for the HPV-DNA (human papillomavirus-deoxyribonucleic acid), Chlamydia and immunohistochemical test; the special liquid base preserving fluid used for the cytologic examination of other parts can be provided, an imported product can be completely replaced, and the cellular constituent with diagnostic significance can be sufficiently preserved; and the exfoliative cells preserving fluid is low in configuration cost and easy to popularize.

The invention discloses a cell cryopreservation fluid which can be used for performing cryopreservation on mesenchymal stem cells or other cells. The cell cryopreservation fluid is prepared from the following ingredients: PBS or normal saline or a basal culture medium serving as a main ingredient, as well as one or more of polyethylene glycol, propanediol, Ectoin, albumin, trehalose, proline and poloxamer 188 which serve as additive ingredients. The cell cryopreservation fluid disclosed by the invention does not contain serums and DMSO, have the definite additive ingredients, and is controllable in quality, high in batch stability and high in safety. By virtue of preserving cells with the cell cryopreservation fluid, revived cells are high in survival rate, and the cellular morphology, the multiplication capacity and the differentiation potential are not influenced; the cell cryopreservation fluid can be used for replacing cryopreservation fluids containing the serums and the DMSO.

This invention relates, e.g., to a composition that, at room temperature, when contacted with a sample comprising phosphoproteins, can fix and stabilize cellular phosphoproteins, preserve cellular morphology, and allow the sample to be frozen to generate a cryostat frozen section suitable for molecular analysis. The composition comprises (1) a fixative that is effective to fix the phosphoproteins, and that has a sufficient water content to be soluble for a stabilizer and / or a permeability enhancing agent); (2) a stabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitor and, optionally, (c) a protease (e.g., proteinase) inhibitor; and (3) a permeability enhancing agent (e.g. PEG). Methods are described for preserving phosphoproteins, using such a composition. Also described are endogenous surrogate markers for monitoring protein degradation, including the loss of posttranslational modifications (such as phosphorylation), e.g. the following removal of a cell or tissue from a subject; and exogenous molecular sentinels (e.g. phosphoproteins attached to magnetic nanoparticles) that allow one to evaluate the processing history of a cellular or tissue population sample.

The invention relates to a preservation solution for exfoliated cells, which is characterized by comprising a pH buffering agent, an osmotic pressure maintaining agent, an anti-corrosion antistaling agent, a fixative for maintaining cellular morphologies, an anticoagulant, a mucus softener, proteinase K and a component for destroying red blood cells. Compared with the prior art, the preservation solution has the advantages that the cell storage rate is high, the storage time at normal temperature is long, the mucus is sufficiently dissolved and the red blood cells are partially destroyed, the sheet-making effect comprises quite even cell distribution, the cellular morphologies are good, the cytolymph and the cell nucleus are divided clearly and are well arranged, the brightness of the cytolymph and the cell nucleus is quite good, and the karyoplasms, the morphological structures and the amount of the cells are quite clear.

The invention discloses a cervical cell pathological slice pathological cell segmentation method and system, in particular, an off-line training sample set of cervical cell pathological slice pathological cell is established, a multi-scale cavity convolution structure semantic segmentation network is introduced on the basis of a depth residual network, and the semantic segmentation model is trained. The unit to be identified is extracted from the cervical pathological slice image, and different types of pathological cells are segmented in the unit to be identified by using the trained semanticsegmentation model. Based on the morphological features of pathological cells, a contour deformation model is established to further optimize the semantic segmentation results. According to the cellnumber and confidence level of different pathological types, the pathological types of the whole slice are predicted. The invention utilizes semantic segmentation network model and deformation variational model to accurately segment different types of pathological cells on the cell pathological slice image, and simultaneously improves the recognition accuracy and the recognition efficiency.

The invention discloses a serum-free culture medium without animal origin components for culturing a Vero cell micro-carrier. The serum-free culture medium consists of a DMEM / F12 (1:1) culture medium and culture medium additive components such as epidermal growth factors, insulin, serotonin, aurin tricar-boxylic acid, biotin, vitamin C, amino acid, fructose, trehalose, trace elements, hydroxypropyl-beta-cyclodextrin and the like. The serum-free culture medium has the following advantages that: (1) the attachment growth of Vero cells in a tissue culture vessel and on the surface of the micro-carrier can be supported, and the cells can be transferred from a serum culture medium to the serum-free culture medium without a course of adaptation; (2) the serum-free culture medium contains no animal origin components, and has basically definite chemical components, and a low cost; and (3) the cells grow well, and the cellular morphology, the density and the vitality are basically the same as those of the serum culture medium.

Three kinds of tissues including cancer tissue, precancerosis and corresponding normal tissue are sliced up, dyed, marked, and positioned. Receptor holes are prepared by leading designed lattice array mould paper to paste on surface of wax block of receptor. Wax block with tissue core bar is prepared by using perforating needle and puncture needle for tissue. Common cancer such as lung cancer, nasopharyngeal carcinoma, oesophagus cancer etc. and having integrated clinical data and pathology features are selected. Through in situ hybridization, testing mRNA of relevant gene and expression of protein on tissue chip, consistent result between the invented product and traditional test is validated. In the product, cellular morphology is clear and even, and there is no fallen off tissue point. The invention is applicable to filter cancers, early diagnosis and forecasting prognosis.

The invention discloses a cryopreservation solution for preserving congenital intrathoracic kidney (CIK) cells and application thereof. The cryopreservation solution for preserving the CIK cells is prepared according to the following methods: adding dimethyl sulfoxide and dextran into a lymphocyte serum-free medium, so that the volume percent content of the dimethyl sulfoxide is 5-15 percent, and the volume percent content of the dextran is 5-15 percent. Compared with the cells before cryopreservation, the cells cryopreserved by employing the cryopreservation solution have the following advantages: (1) the cellular morphology does not have obvious difference; (2) immunophenotype (CD3+CD56+CD8+) does not have obvious difference; and (3) the cell proliferation activity and cell killing activity do not have obvious difference.

Systems and methods in accordance with various embodiments of the invention are capable of rapid analysis and classification of cellular samples based on cytomorphological properties. In several embodiments, cells suspended in a fluid medium are passed through a microfluidic channel, where they are focused to a single stream line and imaged continuously. In a number of embodiments, the microfluidic channel establishes flow that enables individual cells to each be imaged at multiple angles in a short amount of time. A pattern recognition system can analyze the data captured from high-speed images of cells flowing through this system and classify target cells. In this way, the automated platform creates new possibilities for a wide range of research and clinical applications such as (but not limited to) point of care services.

Substrates for influencing the organization, spreading or adhesion of a selected cell to induce or stimulate growth, differentiation or regeneration of the cell or of tissue constituting the cells are provided as well as methods of making such substrates and methods of using such substrates.

The invention discloses a culture medium for establishing a pig iPS cell line and a culture method thereof. A typical pig iPS cell is obtained in the ninth day through transfection by four transcription factors of OCT4, SOX2, KLF4 and c-MYC and induction culture of the culture medium, and the pig iPS cell can be obtained efficiently. The obtained pig iPS cell clone is flat clone, has a regular edge and is similar to the ES cellular morphology of the human body. According to the pig iPS cell line, the pig iPS cell through subculture keeps the undifferentiated state, shows positive in the AP dyeing displaying result and has a pluripotency mark, and the differentiated cell in vitro expresses NCSTN (entoderm), NESTIN (ectoderm) and DESMIN (mesoblast).

The invention provides a method for preparing a decidua mesenchymal stem cell. The method comprises the following steps: performing placenta asepsis by taking the placenta of a male full-term fetus as a raw material; separating a decidual tissue; identifying the decidual tissue and identifying whether the decidua mesenchymal stem cell comes from a maternal tissue; culturing the decidua mesenchymal stem cell; freeze-storing the decidua mesenchymal stem cell; and reviving the decidua mesenchymal stem cell. The method provided by the invention has the technical characteristics that the sampled fetus is male fetus placenta; after the decidual tissue is obtained, sex determination is performed to identify whether the decidual tissue comes from a parent body and is free of pollution of a daughter tissue; by adopting a bacterial pollution prevention method, the pollution possibility is reduced from a sampling source; the surface is washed for a plurality of times, so that the pollution possibility is effectively reduced; a single enzyme is used for digestion to simplify the procedures; a serum-free medium is used for culture to reduce the use of an animal source component; the cell is stable in performance; a long-term in-vitro culture process of the decidua mesenchymal stem cell can be maintained; and the cellular morphology, multiplication capacity, MSC surface marking expression capacity, differentiative capacity and the like of the cell can be maintained.

The invention discloses zaralenone degrading bacteria and application thereof, and belongs to the technical field of biology. ZEN is used as a unique carbon source and energy to carry out preliminary screening and secondary screening, a bacteria strain H6 (CCTCC NO: M2016129) capable of degrading ZEN effectively is separated out, fermentation liquor of the bacteria H6 and ZEN (with final concentration of 10 (mu)g / mL) are cultured for 72 hours under the conditions of 37 DEG C and 180 r / min, and the degradation rate to ZEN reaches 93.64%. Through cellular morphology and physiological and biochemical identification and 16 S rRNA sequence comparative analysis, the strain of bacteria is identified as the bacillus amyloliquefaciens. The degradation active substances of the bacteria strain H6 mainly exist in fermented supernate, thalli also have partial degradation effect. The bacteria strain H6 can be used for preparing a microorganism preparation for degrading zaralenone or a feed biodegradation additive.

The utility model relates to an acquisition and processing system of the cellular morphology image, which aims to construct the image processing platform required by the space cellular embarkation experimental device, comprising a video input processing module, a global logic control module, an image data processing module and an image data transmission module. The video input processing module can convert the analog video signal to digital signal and output the abstracted synchronous signal and pixel clock simultaneously; the global logic control module can realize the logic control of the video input processing and the buffer control of the image data; the image data processing module can complete the acquisition of the cellular morphology image and the cellular image segmentation method based on the edge detection; the image data transmission module can realize the rapid data transmission between the system and the host computer. The utility model has the advantages of integral function, simple structure and flexible programming; in addition, the system can realize the real time online acquisition of the cellular morphology image with high resolution and realize a novel cellular image segmentation method.

The invention particularly relates to a hybrid cell type identification method based on fine granularity identification, comprising the following steps: a fine granularity identification convolutionalneural network model and a cell image database are established in advance; the cell image database comprises a hybrid cell image; the hybrid cell image is an image including a plurality of types of cells; the hybrid cell type identification method comprises the following steps of: 1, collecting mixed cell images; 2, inputting the mixed cell image into a fine-grained recognition convolution neuralnetwork model to obtain a cell type thermogram; 3, performing threshold that mixed cell image to obtain a binary image of the cell region; 4, combined with binary image of cell region and thermogramof cell species, the cell species identification results being obtained. The invention accurately identifies cell species according to the specificity of cell morphological characteristics, and avoidsthe shortcomings of the traditional cell species identification method that takes a long time and the process is tedious. The model can learn the morphological characteristics of fine-grained cells and identify cell types through texture information, which has high recognition accuracy and robustness.

The invention belongs to the field of biotechnology, and especially relates to an in vitro culturing method of goat skeletal muscle satellite cells. The in vitro culturing method comprises following steps: (1) sampling; (2) digestion; (3) filtration; (4) primary culturing; and (5) induction culturing. According to the in vitro culturing method, goat skeletal muscle satellite cells can be quickly obtained via in vitro culturing, and are uniform in cellular morphology, and high in breeding vitality; and after differentiation induction, myotubes can be obtained via fusion of the goat skeletal muscle satellite cells. The in vitro culturing method of goat skeletal muscle satellite cells is simple in operation, is capable of reducing culturing cost greatly, possesses certain economic significance, and can be used for obtaining a large amount of cells with excellent subculturing stability. In vitro culturing of goat skeletal muscle satellite cells is capable of providing exploration on molecular mechanisms of muscle differentiation approaches and production of transgenic animals with improved muscle properties with abundant cell resources, and possesses theoretical values and application values in a certain degree.

The invention discloses cornea mid-term preserving fluid and a preparation method thereof. Based on 1000mL, the mid-term preserving fluid consists of the following ingredients: 4g to 5g of minimum essential medium, 4g to 5g of tissue culture medium 199, 10g to 20g of low-molecular-weight dextranum, 20g to 30g of chondroitin sulfate, 100mg to 150mg of sodium pyruvate, 3g to 4g of HEPES, 20ml to 40ml of calf serum, 10ml to 20ml of non-essential amino acid, 1ml to 2ml of gentamicin sulphate, 100 microliters to 200 microliters of basic fibroblast growth factor and the balance of double distilled water. The cornea mid-term preserving fluid can promote the repair of damaged cells on corneal endothelium and the reconstruction of cellular morphology, can adjust the expression of different integrins on the surface of a cell membrane, can promote the adhesion growth of the cell and can stimulate the proliferation of the endothelial cells, and an effect for reducing the cell apoptosis and maintaining the morphology and activity of the cells can be realized for the mid-term preserved cornea.

The invention discloses a human placenta mesenchymal stem cell suspension protection agent, which is prepared from the following ingredients including human serum albumin, mycose, vitamin C, dextran sodium chloride injection and NaCl solution for injection. The preparation of the human placenta mesenchymal stem cell suspension protection agent is completed through the steps of basic solution preparation, ingredient addition and storage. The activity, the dryness performance and the uniformity of the placenta mesenchymal stem cells can be effectively maintained; the ingredients are specific; the different source animal serum is not contained; the safety is high; in addition, the storage method provided by the invention can effectively maintain the stem cell activity; high efficiency and time saving are realized; the scaled production is facilitated; experiments show that after the human placenta mesenchymal stem cells are stored in the cell storage agent for 48h at 4 to 8 DEG C; the cellular morphology is uniform; the survival rate is still maintained at a value being 90 percent or higher; the mesenchymal stem cell specific surface mark CD73, CD105 and CD90 expression rate is higherthan 95 percent.

The invention discloses an underwater transparent silicon dioxide nanofiber substrate as well as a preparation method of the substrate and application of the substrate to capture of circulating tumor cells. The preparation method of the substrate comprises the following steps: coating the surface of the substrate with a hydrolyzed tetraethoxy polymer solution by virtue of an electrospinning technique, then carrying out heat preservation by a drying box for further hydrolyzing; and finally burning the substrate at the high temperature to obtain the underwater transparent silicon dioxide nanofiber substrate; the substrate can be used for enriching and detecting circulating tumor cells after being further modified by a biochemistry surface antibody. The substrate has the characteristics of being high in efficiency of capturing the circulating tumor cells and high sensitivity and can be applied to clinical diagnosis of patients with cancer; meanwhile, the tumor cells captured by the substrate are high in activity; the substrate can be used for monitoring in real time and providing relevant information such as cellular morphology and has potential significance to the subsequent research of the tumor cells.

Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s)of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.

The invention discloses a high-activity primary cartilage cell preparing method. The high-activity primary cartilage cell preparing method includes the following steps that 1, cartilage tissues of joints in limbs of an immature rat are extracted; 2, a blade special for operations is adopted to cut up the cartilage tissues; 3, the cut-up cartilage tissues are placed in a constant temperature shaking table to be shaken and placed in a horizontal centrifuge for centrifugation by 1500-2000 rpm, cell sediments are left, the cartilage tissues continue to be placed in the shaking table to be digested, are separated from cartilage matrixes after being repeatedly blown and beaten by a gun head of 1 ml, and are placed in the horizontal centrifuge for centrifugation by 1500-2000 rpm, and cartilage cell sediments are left; and 4, the cartilage cell sediments are planted in a culture dish and cultured in a DMEM high-glucose culture medium containing 10% of FBS to obtain high-activity primary cartilage cells. Compared with a traditional cartilage cell extracting method, the high-activity primary cartilage cell preparing method shortens the time by a half and improves the yield of cartilage cells by at least ten times; experiments verify that the obtained cartilage cells have a good rate of increase and good cellular morphology.

The invention discloses a separation method of human placenta mesenchymal stem cells. The separation method comprises the following steps that placenta specimen treatment is carried out, treated placenta tissue is obtained, tissue digestion is carried out on the placenta tissue, and cell suspension is obtained; preliminary subculture of the placenta mesenchymal stem cells is carried out; further separation and culture of the placenta mesenchymal stem cells are carried out. HyQTase and DNAse I are adopted for digestion together, the placenta mesenchymal stem cells are obtained through separation, after preliminary culture, microgravity treatment and electromagnetic field and sound wave treatment are carried out, BMP4 is used for treatment many times, upper layers are removed through a differential attachment method, a serum-free medium and antioxidant are replenished in a culture bottle for culture, and the final purity is high. The stem cell characteristics of the cells are verified through detection on hereditary stability, cell surface molecule expression conditions and cellular morphology in the continuous passage process, and materials are provided for seed cells with the placenta mesenchymal stem cells as clinical application.

The invention relates to the technical field of a medicine preparation, and particularly relates to a kit for screening abnormal cervical cells, and enzyme-labeled liquid based cell preservation solution. The kit for screening abnormal cervical cells has excellent sensitivity and good specificity; and the enzyme-labeled liquid based cell preservation solution disclosed by the invention can well keep acid phosphatase activity of cervical exfoliated cells and the cellular morphology, and has the functions of preventing corrosion, removing impurities and dissolving red blood cells; and the effects of tabletting and dyeing the stored cells are superior to the effect of the traditional preservation solution during the usage period.

The invention discloses bacillus and application thereof to production of acetoin and 2, 3- butanediol through high temperature fermentation. A strain is Bacillus sp. H15-1CGMCC No. 12389, the gram faerbung is positive, spores are produced, the cellular morphology is in a rod shape, and the stain can grow at the temperature of 40-60 DEG C and proliferate at the highest speed at the temperature of 50 DEG C. By means of the strain, degerminated corn flour hydrolysate is adopted as the main raw material, a mechanical stirring and ventilation fermentation tank is adopted for conducting fermentation for 68 hours at the temperature of 50 DEG C, and acetoin with the concentration being 50.8 g / L and 2, 3- butanediol with the concentration being 32.1 g / L can be generated. A method for producing acetoin and 2, 3- butanediol through high temperature fermentation has the advantages that raw materials are low in cost, the fermentation process is resistant to infectious microbe contamination, cooling water is saved, and operation is conducted coarsely.

The invention discloses a method for observing same cellular morphology by ordinary optical, fluorescence and scanning electron microscopes. The method is characterized by embedding a sample with paraffin, slicing the tissue of the embedded sample, and determining the slicing progress according to preliminary observation; using the microscopes to observe obtained slices by dyeing of the tissue with a fluorescent composite dye under excitation of white light and different bands of fluorescence to obtain observation results; treating the remaining embedded sample and observing by the scanning electron microscope to obtain a sample section scanning result. The basic morphology, size and basic structure of a cell can be obtained by the ordinary optical microscope, some special structure of thecell can be obtained by the fluorescence microscope, and a three-dimensional image of the interior of the cell can be obtained by the scanning electron microscope. The method is very helpful for thestudy of cell morphology development, as well as the cell internal structure, especially cell nucleus morphology and function.

The invention provides a human placenta mesenchymal stem cell, a preparation method and an application thereof. The preparation method comprises the following steps: collecting, separating, culturing,cryopreserving, detecting, recovering, and the like. A high-purity high-activity mesenchymal stem cell can be acquired by only performing slide adherent culture after a human placenta chorion tissueblock is acquired in the separating process; the technological process is simplified; the cost of enzymic digestion is saved; the adherence and growth rate of primary cells are accelerated; the cell culture period is shortened; the introduction of more external interference factors is avoided, so that the process stability can be easily controlled. The multiplication capacity of the human placentamesenchymal stem cell acquired according to the invention is more stable than that of other mesenchymal stem cells; after the human placenta mesenchymal stem cell passes to P20 generation, the cell still can stably proliferate, and the cellular morphology, molecular surface antigen and adipogenesis osteogenesis differentiative potential thereof all meet the regulations for minimum standard of MSCidentification from international cell therapy association.