18 results about "Peste-des-Petits-Ruminants" patented technology

The invention discloses a kit for simultaneously detecting bluetongue virus, foot and mouth disease virus, schmallenberg virus, peste des petits ruminants virus and sheep pox virus, and belongs to the technical field of virus detection. The kit comprises primers as shown in SEQ ID NO. 1 to SEQ ID NO. 18 and a probe as shown in SEQ ID NO. 18. After a single system of bluetongue virus, foot-and-mouth disease virus, schmallenberg virus, peste des petits ruminants virus and capripox virus is tested, a sextuple system containing an interior label is established, and through testing of indexes such as linearity, minimum detection limit, specificity, precision and repeatability, NTC repetition, comparison of multiple fluorescent RT-PCR and single fluorescent RT-PCR, the kit can be used for detecting the virus resistance of the peste des petits ruminants virus and the capripox virus. The currently developed fluorescent RT-PCR multiple detection kit for the sheep respiratory tract syndromes completely meets the development requirement, the specificity is high, the sensitivity is high, and the lowest detection limit can reach 10 Copi / mu L or below.

The invention relates to a primer-probe set, comprising a forward primer having the nucleotide sequence of SEQ ID NO:1 and a reverse primer having the nucleotide sequence of SEQ ID NO:2, and a peptide nucleic acid (PNA) probe having the sequence of HEX-OO-(SEQ ID NO:3)-Lysine-BHQ1, which are used for differentiating the Peste des Petits Ruminants (PPR) virus vaccine and field strains and for preventing false positive results arising from the PPR vaccine, as well as to their operating temperatures. Said primer-probe set can differentiate between the vaccine strain containing the nucleotide sequence of SEQ ID NO:4 and the field strain containing the nucleotide sequence of SEQ ID NO:5. Thus, by means of said primer-probe set, false positive results arising from the PPR vaccine are prevented, and accordingly, unnecessary quarantine measures are also avoided.

The invention discloses a single-chain antibody for recognizing an extracellular domain of a PPRV H protein and application of the single-chain antibody, and belongs to the technical field of genetic engineering. The amino acid sequence of a heavy chain variable region of the single-chain antibody is as shown in SEQ ID NO.5, and the amino acid sequence of a light chain variable region of the single-chain antibody is as shown in SEQ ID NO.9. The invention provides a single-chain antibody for recognizing a PPRV H protein extracellular region (185-609aa), a mouse is immunized through a PPRV H protein extracellular region protein, and VH and VL genes of the immunized monoclonal antibody are connected in series by using a monoclonal antibody preparation technology and a DNA molecular technology to prepare the single-chain antibody. A neutralization test verifies that the single-chain antibody can neutralize PPRV viruses and can be used for clinical detection of peste des petits ruminants.

The invention discloses a monoclonal antibody 5B11 for recognizing an extracellular domain of peste des petits ruminants virus H protein and application of the monoclonal antibody 5B11, and belongs to the technical field of monoclonal antibodies. According to the invention, PPRV-H-185 protein with an amino acid sequence as shown in SEQ ID NO.1 is used for immunizing a mouse, a monoclonal antibody 5B11 with a neutralizing effect is prepared, a heavy chain variable region of the monoclonal antibody 5B11 comprises CDR1-3 with an amino acid sequence as shown in SEQ ID NO.7-9, and a light chain variable region of the monoclonal antibody 5B11 comprises CDR1-3 with an amino acid sequence as shown in SEQ ID NO.12-14. Experimental results show that the monoclonal antibody can be combined with a virus, so that the virus loses the ability of combining an epithelial cell H protein receptor Nectin-4 and a lymphocyte H protein receptor SLAM, and the virus is prevented from invading cells. The invention provides a new biological material for detection and prevention of peste des petits ruminants virus, and has a good clinical application prospect.

The invention discloses an application of an intestinal strain in preparation of a cholesterol-lowering product, and belongs to the technical field of microbial medicines. The intestinal bacterial strain is Oscillibacter ruminant, the viable bacteria concentration of the intestinal bacterial strain is controlled to be (4.8-5.2) * 10 < 9 > CFU / mL, the intestinal bacterial strain can be prepared into bacterial liquid, freeze-dried powder, capsules or tablets and other dosage forms, and pharmaceutically acceptable auxiliary materials such as lactose and microcrystalline cellulose can be added. According to the cholesterol-lowering product, the total cholesterol, triglyceride and low-density lipoprotein cholesterol level of an object can be remarkably reduced under the action of peste des petits ruminants, and meanwhile, the high-density lipoprotein cholesterol level is increased; in addition, abnormal blood glucose induced by high-fat diet can be improved, arterial lipid accumulation is reduced, and the plaque area proportion of atherosclerotic lesions is improved.

The application discloses an RNA vaccine, wherein the RNA vaccine is prepared by taking the protein sequence of the H gene and the F gene of a small ruminant pestivirus strain with the microbial preservation number of CCTCC NO:V202487 as an immunogen, and has good immunogenicity and immunoprotective power, and has an application prospect.

The application belongs to the technical field of virus detection, and discloses a digital PCR kit for simultaneously detecting bovine dermatophilosis virus, Schmallenberg virus and peste des petits ruminants virus, which comprises primers and probes shown as SEQ ID NO. 1-SEQ ID NO. 18. After single system tests on bovine dermatophilosis virus-ORF101 gene, bovine dermatophilosis virus-ORF126 gene, Schmallenberg virus, peste des petits ruminants virus-N gene and peste des petits ruminants virus-M gene, a six-repeated digital PCR system containing an internal standard is established. After tests on indexes such as linearity, specificity, minimum detection limit, repeatability, single and multiple digital PCR systems, the developed digital PCR multiple detection kit can fully meet the development requirements, has strong specificity, high sensitivity, and the minimum detection limit can reach below 10 Copies / μL.

The invention discloses a peste des petits ruminants virus strain with a microbial preservation number of CCTCC (China Center For Type Culture Collection) NO: V202487. A DNA vaccine and an RNA vaccine which are prepared by taking proteins coded by the H gene and the F gene of the strain as immunogens have good immunogenicity and immune protection capacity. The strain has an application prospect.

The invention belongs to the technical field of biology, and relates to a monoclonal antibody specifically combined with peste des petits ruminants virus N protein and application thereof. The sequence of the monoclonal antibody CDR1-H is as shown in SEQ ID NO: 1; the sequence of the CDR2-H is as shown in SEQ ID NO: 2; the sequence of the CDR3-H is as shown in SEQ ID NO: 3; the sequence of the CDR1-L is as shown in SEQ ID NO: 4; the sequence of the CDR2-L is LMS; the sequence of the CDR3-L is as shown in SEQ ID NO: 5. The monoclonal antibody can be subjected to specific reaction with peste des petits ruminants virus or N protein of the peste des petits ruminants virus; the enzyme-labeled monoclonal antibody is used as a detection antibody, a double-antibody sandwich ELISA method for detecting the peste des petits ruminants virus is established, the method is good in specificity and high in sensitivity, and the coincidence rate with a commercial kit is as high as 93.75%.

The invention discloses an alfalfa stress-resistant high-yield breeding method based on a microbiome regulation and control technology, and belongs to the technical field of microbial breeding. According to the breeding method, compound fertilizer doped with a microbial agent is applied in a seedling stage, a branching stage and a squaring stage respectively, and the compound fertilizer comprises a substrate and the microbial agent, the microbial agent is prepared from sinorhizobium meliloti, bacillus stearothermophilus, peste des petits ruminants, bacillus licheniformis, azotobacter brown, bacillus siamensis and bacillus amyloliquefaciens; the substrate comprises at least two of cow dung, pig manure, bean bran, corn straw and wood chips. According to the breeding method, the new microbial composition is prepared for preparing the compound fertilizer, the nutrition absorption capacity of the alfalfa can be improved through a microbial regulation function, and then the stress resistance and yield of the alfalfa are improved.

The application discloses a kind of PPRV-F nanobody and nanobody preparation, purification and neutralization test method, and wherein, the PPRV-F nanobody includes the amino acid sequence shown in SEQ ID NO:1.The preparation and purification method of the nanobody provided in the application can obtain the PPRV-F nanobody with neutralization titer reaching 1:20.The nanobody prepared in the application can be used for the treatment, prevention of PPR and the detection of PPRV.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (Polymerase Chain Reaction) kit for simultaneously detecting bovine nodular skin disease virus, schmallenberg virus and peste des petits ruminants virus, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.18. The invention also discloses a kit for simultaneously detecting bovine nodular skin disease virus, schmallenberg virus and peste des petits ruminants virus. According to the invention, a sextuple digital PCR (Polymerase Chain Reaction) system containing an internal standard is established after a single system of a bovine nodular skin disease virus-ORF101 gene, a bovine nodular skin disease virus-ORF126 gene, a schmallenberg virus, a peste des petits ruminants virus-N gene and a peste des petits ruminants virus-M gene is tested; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The invention discloses a DNA vaccine, and the DNA vaccine prepared by taking an H gene and an F gene of a peste des petits ruminants virus strain with a microbial preservation number of CCTCC NO: V202487 as coding genes of immunogens has good immunogenicity and immune protection capability, and has an application prospect.

This invention relates to the field of biotechnology, specifically to a monoclonal antibody against the non-structural protein V of peste des petits ruminants (PPR) virus and its gene sequence. The invention discloses a monoclonal antibody 3E10 against PPR non-structural protein V, wherein the sequence of monoclonal antibody 3E10 includes the heavy chain variable region shown in SEQ ID NO.7 and the light chain variable region shown in SEQ ID NO.8. Monoclonal antibody 3E10 specifically reacts with PPR and exhibits high affinity, enabling its detection of PPR. This provides new material for functional studies of PPR V protein and the development of diagnostic reagents, and offers new technical support for the successful implementation of the global PPR elimination program.

The application provides a use of remdesivir in preparation of a preparation for preventing and / or treating peste des petits ruminants virus, and belongs to the technical field of biological medicine. The application finds that the use of remdesivir in a safe concentration range can significantly inhibit the replication of the peste des petits ruminants virus in host cells, obviously reduce the expression level of virus-related genes and proteins, and exhibit good antiviral activity. Meanwhile, the inhibitory effect of remdesivir on the PPRV presents obvious dose dependence, and with the increase of the acting concentration, the antiviral effect is significantly enhanced, which indicates that the effect is stable and has strong controllability. Moreover, remdesivir mainly acts on the virus replication stage, can effectively block the virus replication process, thereby reducing the virus infection level and reducing the generation of daughter virus particles. The application provides a new technical approach for the prevention and treatment of peste des petits ruminants, makes up for the deficiency of lack of effective antiviral drugs in the prior art, and has a good application prospect.

The invention belongs to the technical field of biotechnology detection, and relates to a primer probe combination for identifying peste des petits ruminants vaccine strains and wild strains and a detection method of the peste des petits ruminants vaccine strains and the wild strains. The invention discloses a primer pair and a probe for identifying a peste des petits ruminants vaccine strain and a wild strain. The primer pair comprises an upstream primer and a downstream primer, wherein the upstream primer is PPRV-qF: TCGGAAGAACATACYGTC, and the downstream primer is PPRV-qR: CRACTCGAAGACTCTTTAA; the nucleotide sequence of the probe for detecting the peste des petits ruminants vaccine strain is FAM-ACCGAGCACCAGTCCAGGTAT-BHQ1, and the nucleotide sequence of the probe for detecting the peste des petits ruminants vaccine strain is The nucleotide sequence of the probe for detecting the peste des petits ruminants wild strain is HEX-ATCCGAGCACCGATCTAGGTATC-BHQ1, and the nucleotide sequence of the probe for detecting the peste des petits ruminants wild strain is HEX- The peste des petits ruminants vaccine strain and the wild strain can be identified and detected only by a pair of primers and two probes marked by different fluorophores.

This invention belongs to the field of goat vaccine technology and is an application of a vaccine for the prevention of small ruminant plague (PPR) and sheep pox. This PPR-sheep pox vaccine is an improvement on the injection method of a bivalent live PPR-sheep pox vaccine, suitable for doubling the intramuscular injection in goats. Each injection of 1 ml of the bivalent live PPR-sheep pox vaccine results in a population antibody titer of 70% for both PPR and sheep pox after 30 days, and sheep pox antibody titer can still be detected after 180 days. This PPR-sheep pox vaccine demonstrates good safety and PPR immunity protection in Alashan white cashmere goats. Furthermore, the vaccine is suitable for intramuscular injection, which meets the needs of sheep pox immunization and control, and is suitable for practical grassroots disease prevention operations. It provides important technical support for the scientific prevention and control of diseases in regional white cashmere goats. Promoting the use of this PPR-sheep pox vaccine can prevent both PPR and sheep pox.

The invention provides a primer probe composition for detecting peste des petits ruminants virus and application thereof, and relates to the technical field of biological detection, the primer probe composition comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO: 33, a downstream primer with a nucleotide sequence as shown in SEQ ID NO: 41, and a specific fluorescent probe. The invention provides the primer probe for detecting the peste des petits ruminants virus based on the RPA, and solves the technical problem that the RPA primer for detecting the peste des petits ruminants virus is lacked in the prior art.