308 results about "Bacterial protein" patented technology

The invention applies to the tea crop production, which can improve the soil structure and provide adequate moisture, oxygen and nutrition to improve the yield and quality in tea crop cultivation. The invention relates to a liquid fertilizer containing microorganisms, organic matters and inorganic matters. The fertilizer adopts biological technology and electrolysis technology which complement each other, takes micro-organisms and mineralized substances as additive, as well as combines with nutritional formula (nitrogen, phosphorus, potassium, sulfur, magnesium, calcium, silicon, boron, zinc, iron, molybdenum, bacterial protein, amino acid, humus, plant nucleic acid, B vitamins, active enzymes and unknown factors which promote crop growth), so as to provide adequate nutrition for crops and improve soil to make the soil conducive to tea crop cultivation. Through biotechnology, the micro-organisms in a dormant state are added to the fertilizer and are activated under favorable environmental conditions to decompose the animal and plant residues, pesticides, fertilizers, heavy metal residues and minerals in the soil so as to change the contents of nitrogen, phosphorus and potassium. Through electrolysis technology, the mineralized substance additive enables the soil to produce a chain reaction to moisture and oxygen supply, so as to gradually change the ion charge in the soil, increase the soil oxygen supply amount, improve the moisture infiltration capacity and strengthen the conditions for microbial activities.

An antibacterial antisense conjugate and method of using the same for treating a bacterial infection in a mammalian host are disclosed. The conjugate includes an antisense oligonucleotide conjugated to a carrier peptide that significantly enhances the antibacterial activity of the oligonucleotide. The antisense oligonucleotide contains 10-20 nucleotide bases and has a targeting nucleic acid sequence complementary to a target sequence containing or within 10 bases, in a downstream direction, of the translational start codon of a bacterial mRNA that encodes a bacterial protein essential for bacterial replication, where the compound binds to a target mRNA with a T_m of between 50° to 60° C. The carrier peptide is an arginine-rich peptide containing between 6 and 12 amino acids.

The invention relates to escherichia coli-derived single-plasmid-tandem soluble coexpression foot-and-mouth disease virus capsid proteins VP0 (which is a VP4 and VP2 fusion gene), VP1 and VP3, and a foot-and-mouth disease virus capsid protein virus-like particle preparation method. Foot-and-mouth disease virus capsid protein virus-like particles can be used for preparation of a foot-and-mouth disease vaccine. According to the method, a plurality of aspects of escherichia coli-derived soluble coexpression foot-and-mouth disease virus capsid protein are studied, by comprehensive use of tandem coexpression and SUMO(suggested upper merged ontology) technology with a tag for soluble coexpression of the foot-and-mouth disease virus capsid proteins VP0 (which is the VP4 and VP2 fusion gene), VP1 and VP3, the ultimate objective protein accounts for about 20% of total bacterial protein, and the foot-and-mouth disease virus capsid proteins obtained by purification can be successfully assembled into the virus like particles.

A method and antisense compound for inhibiting the growth of pathogenic bacterial cells are disclosed. The compound contains no more than 12 nucleotide bases and has a targeting nucleic acid sequence of no fewer than 10 bases in length that is complementary to a target sequence containing or within 10 bases, in a downstream direction, of the translational start codon of a bacterial mRNA that encodes a bacterial protein essential for bacterial replication. The compound binds to a target mRNA with a T_m of between 50° to 60° C. The relatively short antisense compounds are substantially more active than conventional antisense compounds having a targeting base sequence of 15 or more bases.

The present invention is directed to oligonucleotide-modified nanoparticle conjugates and methods of inhibiting bacterial protein production.

The invention applies to the tobacco crop growth, which can improve the soil structure and provide adequate moisture, oxygen and nutrition to improve the yield and quality in tobacco crop cultivation. The invention relates to a fertilizer containing microorganisms, organic matters and inorganic matters. The fertilizer adopts biological technology and electrolysis technology which complement each other, takes micro-organisms and mineralized substances as additive, as well as combines with nutritional formula (nitrogen, phosphorus, potassium, sulfur, magnesium, calcium, silicon, boron, zinc, iron, molybdenum, bacterial protein, amino acid, humus, plant nucleic acid, B vitamins, active enzymes and unknown factors which promote crop growth), so as to provide adequate nutrition for crops and improve soil to make the soil conducive to the cultivation of tobacco crops. Through biotechnology, the micro-organisms in a dormant state are added to the fertilizer and are activated under favorable environmental conditions to decompose the animal and plant residues, pesticides, fertilizers, heavy metal residues and minerals in the soil so as to change the contents of nitrogen, phosphorus and potassium. Through electrolysis technology, the mineralized substance additive enables the soil to produce a chain reaction to moisture and oxygen supply, so as to gradually change the ion charge in the soil, increase the soil oxygen supply amount, improve the moisture infiltration capacity and strengthen the conditions for microbial activities.

The present invention relates to methods and systems for administering antibody therapeutic agents. The methods include administering one or more (e.g., two or three) binding agents, wherein each of the binding agents has a binding region that is specific to a portion of a disease agent and one or more copies of a tag. The binding agents can be specific to one or more portions of the same or different disease agents. The tag is the same for each of the binding agents. The methods include administering an anti-tag antibody, wherein the anti-tag antibody has an anti-tag region that is specific to the tag, and can have an immunoglobulin (e.g., IgA, IgD, IgE, IgG, and IgM.). Disease agents include bacterial proteins, viral proteins, cancer cells, and proteins or toxins produced therefrom. In particular, the present invention includes methods and systems for binding agents that are specific to neurotoxins that cause botulism.

Antibacterial antisense compounds and methods of their use in treating a Mycobacterium tuberculosis infection in a mammalian host are disclosed. The compounds include an antisense oligonucleotide conjugated to a carrier peptide that significantly enhances the antibacterial activity of the oligonucleotide. The antisense oligonucleotides contain 10-20 nucleotide bases and have a targeting nucleic acid sequence complementary to a target sequence containing or within 20 bases, in a downstream direction, of the translational start codon of a bacterial mRNA that encodes a bacterial protein essential for bacterial replication, where the compound binds to a target mRNA with a T_m of between 45° to 60° C. The carrier peptide is an arginine-rich peptide containing between 6 and 14 amino acids. Antisense compounds that target host factor genes that facilitate Mycobacterium tuberculosis infection are also provided, as are methods of using these compounds to treat Mycobacterium tuberculosis infections, alone or in combination with other therapies.

This invention relates to composition and methods of employing said composition for treating or preventing microbial associated infections and diseases. More particularly the present invention relates to bacterial proteins, peptides and amino acids which are by-products of bacteria, in particular Lactobacillus and more specifically Lactobacillus strains GR-1 and RC-14, in compositions that can treat and prevent microbial-associated infections and diseases, by altering, for example, down-regulating, virulence properties of pathogenic organisms.

The invention discloses an anti-bacterial protein PBR1 as well as a preparation method and application thereof, and relates to the technical field of molecular biology and biological pesticides. The amino acid sequence of the anti-bacterial protein PBR1 is shown as SEQ ID NO.1. The anti-bacterial protein PBR1 has a superior effect on preventing and curing plant diseases such as cruciferous plasmodiophoromycetes, paddy rice magnaporthe oryzae, curvularia lunata, fusarium oxysporum f. sp. dianthi, fusarium oxysporum vasinfectum, konjac foot rot bacteria, panax notoginseng root rot pathogen, pomegranate wilt pathogen, gaeumannomyces gramini, cylindrosporium pomi brooks, alternaria alternata and/or botrytis cinerea. A pesticide taking the anti-bacterial protein PBR1 as an active ingredient is a novel environment-friendly biological pesticide, is safe to people and livestock, protects the environment, and has a favorable development prospect.

The invention discloses microbial fermentation feed. The microbial fermentation feed is prepared from, by weight, 3-5 parts of a complex microbial agent, 680-730 parts of a fermentation raw material,275-325 parts of an energy raw material, 350-400 parts of water and 1-2 parts of brown sugar by mixed fermentation. The complex microbial agent is made by mixing of 5-6 of bacillus subtilis, enterococcus faecalis, plant lactobacillus, bacillus pumilus, clostridium butyricum, bacillus megaterium, bacillus amyloliquefaciens or lactobacillus casei. By fermentation treatment of the fermentation raw material and the energy raw material with the complex fermentation strain, enzymolysis of coarse fibers in the fermentation raw material is realized, organic nitrogen and inorganic nitrogen, which can be hardly used by animals, in the fermentation raw material are converted into bacterial protein, and macromolecular carbohydrates, which cannot be absorbed by the animal, in the energy raw material are converted into absorbable low-molecule carbohydrates.

The invention relates to a bacterial protein feed, a production process and applications thereof. The bacterial protein feed contains clostridium autoethanogenum which can be used for animal feeds. The production process of the bacterial protein feed includes the following steps: preparation of raw material gas containing CO, clostridium autoethanogenum fermentation, distillation, solid-liquid separation, drying, inspection and packaging so as to obtain the clostridium autoethanogenum protein feed. The bacterial protein feed obtained by the process of the invention is a single strain, and is high in product purity and rich in 18 amino acids and all amino acids necessary for animals, and the clostridium autoethanogenum protein can be used as an animal feed raw material. The feed is green and environmentally friendly in production process, and is free from antibiotic and pesticide residue compared with traditional plant proteins or animal proteins.

The invention belongs to the technical field of production of amino acids, and relates to a process for extracting high-quality L-malic acid in biological fermentation liquor by acidolysis, neutralization, grading film filtration and ion exchange technologies. The process comprises the following steps: acidizing by using phosphoric acid, filtering with micro-filtration membranes and ultrafiltration membranes to remove bacterial protein, other macromolecule impurities, calcium phosphate and other solids; neutralizing fermentation liquor by using calcium hydroxide and calcium carbonate, acidizing by using phosphoric acid, filtering with micro-filtration membranes and ultrafiltration membranes to create a decisive condition for ion exchange and concentration crystallization of a cooling evaporator of the L-malic acid. According to the process, the purity of the L-malic acid extracted once is greater than 99%, the total yield is above 90%, the phosphoric acid is of moderate acidity, and membrane filtering equipment is high in automation degree, stable in operation and convenient for industrial popularization, so that the process has favorable economic benefit, environmental benefit and application prospect.

The invention discloses composite feed for dairy cow and consists of the following raw materials: straw of 20 to 28 parts by weight (calculated by dry matter), carrot of 0.8 to 1.2 parts by weight (calculated by dry matter), degradation bacteria of 500 to 1500 ml per ton straw (calculated by dry matter), brown sugar of 0.8 to 1.5 kg per ton straw (calculated by dry matter) and adequate water. After preparation, rubbing and crushing, mixing, spraying, compressing and baling, thin film packaging, sealing and fermentation, inspection and storage processes, compound feed favorite for dairy cow is produced. The invention feed has sourness and incense of silage and sweet and sourness of carrot and has high bacterial protein content, is rich in vitamin, meanwhile, having high nutritional value and good palatability, being eaten very fondly by dairy cow and other grazing animal, providing a good selection to dairy cow as picky eater from the most critical aspect, facilitating the preservation and transportation, providing the breeding of local dairy cow with high-quality functional roughage and having relatively low processing cost and broad market prospect.

The invention relates to a method for producing a high-efficiency biological peptide through enzymolysis and microbe fermentation, which comprises the following steps of: smashing a fermentation substrate, uniformly mixing, then adding water for material blending, and then cooking and sterilizing; when temperature is reduced to 50-55 DEG C, adding a proteolytic enzyme for hydrolyzation; after the hydrolyzation is finished, when the temperature is reduced to 28-35 DEG C, adding a mixed bacterium solution to the hydrolyzed fermentation substrate, uniformity stirring, and carrying out aerobic fermentation under the condition of constant temperature; and carrying out low-temperature drying on the fermented materials, and then smashing to obtain a high-efficiency biological peptide finished product. The fermentation substrate comprises the following materials in parts by weight: 50-60 parts of high-protein bean pulp, 10-20 parts of glutamic acid bacterial protein and 30-40 parts of dephenolized cottonseed protein, and the components are smashed and screened through a 40-mesh screen and then uniformly mixed. The mixed bacterium solution is obtained by mixing the bacterium solutions of aspergillus niger, candida tropicalis, aspergillus oryzae and bacillus subtilis according to the proportion of 1:1:1:1, and the mixed bacterium solution is inoculated into the hydrolyzed fermentation substrate in 5%-10% by weight.

The invention relates to a method for obtaining a nitrogen source for producing riboflavin. The method comprises the following steps: performing desalination treatment on wastewater of a riboflavin fermentation broth after extraction, wherein the total salt content of the wastewater is controlled to be less than 5g/l after desalination; adding 2-5% of polymerized ferrous sulfate for flocculating for 1-2 hours, centrifuging for filtering by using a double-conical decanter centrifuge so as to obtain a solid substance, and drying the solid substance so as to obtain bacterial protein. By adopting the method, the problem that the riboflavin bacterial protein is hard to utilize because of high salt content is solved, the bacterial protein which can be alternatively used as a fermentation nitrogen source is obtained, the application of the expensive organic nitrogen source is greatly reduced, and recycling and utilization of resource are achieved. Besides, on the basis of the bacterial protein, the invention further discloses a corresponding seed culture medium formula and a fermentation culture medium formula, and the process control conditions are optimized starting from factors including temperature, pH values, residual sugar content, dissolved oxygen content, ammonia feeding amount, material supplement mode and the like which can affect the stability of fermentation.

A method for preparing the bacterial protein forage includes such steps as providing distillers' grains, straw or stalk, bran and corn powder, stirring, adding ammonium sulfate and urea, regulating pH=5.5-6.5 by lime milk, inoculating the liquid mixture of reticularia, candida utilis and Rhizopus, and fermenting at 28-32 deg.C for 18-24 hr in the ventilation condition.

The invention relates to the technical field of microbes, and specifically relates to trichoderma koningii, and a compound microbial agent composition and an application thereof. The invention discloses trichoderma koningii HK43-5 (CGMCC No7422), a compound microbial agent composition composed of a glycosylated bacterial preparation and a forage yeast preparation which are packaged separately, wherein the glycosylated bacterial preparation comprises the trichoderma koningii HK43-5 (CGMCC No7422). Cellulose produced by the trichoderma koningii HK43-5 (CGMCC No7422) has high activity, can be used as an efficient glycosylated bacterial preparation in production of industrial and agricultural residual protein forage. The compound microbial agent composition can efficiently utilize industrial and agricultural residues, particularly potato residues, to produce single cell protein. Crude protein content in fermented mycoprotein is 50.49% which is far superior to that reported in a conventional technology. The industrial and agricultural residues are changed to wealth; and the produced bacterial protein can be applied in the forage to partly substitute soybean meal or fish meal. At the same time, the environment pollution problems resulted from the industrial and agricultural residues are solved.

The invention relates to a method for extracting red date cyclic adenosine monophosphate through an ultrasonic composite membrane technology. The method solves the problem that the existing extraction technology has the defects of complex processes, low extraction rate, high industrialization difficulty and low product purity. The method comprises the following steps of removing date pits of cleaned red dates, crushing the red dates obtained by the previous step, adding an appropriate amount of an ethanol solvent into the crushed red dates, carrying out ultrasonic reinforcement extraction, repeatedly carrying out ultrasonic extraction of extracted filter residues, merging the extract obtained by the previous step, removing macromolecules of bacteria, proteins, pectin, polysaccharides and starch, and monosaccharides of glucose and fructose respectively through ultrafiltration and nanofiltration, intercepting cyclic adenosine monophosphate-rich concentrate, and carrying out pressure reduction condensation and spray drying to obtain 30% of a powdery cyclic adenosine monophosphate crude product. The method provided by the invention is easy for operation, is convenient for extraction, is fast, has short extraction time and a high cyclic adenosine monophosphate extraction rate, and is suitable for industrialized production. A product obtained by the method has high purity, is purely natural and does not produce chemical pollution. Therefore, the method provides an effective approach for comprehensive development of red date further processing.