49 results about "Bacterial protein" patented technology

The invention discloses a preparation method of a fructus cannabis protein peptide product with effects of resisting fatigue and improving exercise ability, which comprises the following steps: performing preliminary crushing, ultralow-temperature freezing micro-crushing and low-temperature continuous phase-change extraction degreasing on fructus cannabis, performing step-by-step hydrolysis by using polysaccharase and 2.4 L bacterial protease to extract fructus cannabis protein, performing low-temperature salting-out, and performing freeze-drying to obtain the fructus cannabis protein peptide product with the effects of resisting fatigue and improving exercise ability. The fructus cannabis protein peptide powder is prepared through low-temperature ultrasonic dissolution promotion, high-pressure micro-jet homogenization, compound enzyme C hydrolysis, 1% pepsase, high-temperature enzyme deactivation, chlorophyll removal through counter-current extraction, hydrogen peroxide decoloration, ultralow-temperature instantaneous concentration, low-temperature vacuum belt type drying and low-temperature ultrahigh-pressure sterilization. The fructus cannabis protein peptide prepared by the invention has the following remarkable effects: 1, the exercise ability is remarkably improved, the accumulation of lactic acid and urea nitrogen is reduced, the exhaustion time is prolonged, and the physical and mental fatigue is relieved; 2, the content of hepatic glycogen and muscle glycogen is obviously increased, the energy reserve of the body is increased, the explosive power and the reactivity are improved, and the athletic performance is improved; and 3, the antioxidant level of the body is improved, and fatigue and immune recovery after exercise are promoted.

This invention relates to a 1,3,4-thiadiazole peptide deformylase inhibitor and its preparation and application. The structural formula is shown in formula (1): wherein, R 1 It is n-butyl or cyclopentylmethyl; R 2 The compounds are hydrogen-containing, straight-chain alkanes, cyclic alkanes, aromatic rings, substituted biphenyls, or heterocyclic rings. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention can effectively inhibit bacterial protein synthesis, thereby achieving sterilization. These compounds exhibit excellent inhibitory activity against Gram-positive drug-resistant bacteria, especially the clinically challenging methicillin-resistant Staphylococcus aureus (MRSA). In particular, some preferred compounds show inhibitory activity 4-8 times that of the control standards vancomycin and linezolid. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention also exhibit inhibitory activity against Gram-negative drug-resistant bacteria, especially against drug-resistant Acinetobacter baumannii, known as "superbugs," with an inhibitory activity reaching 0.5 μg / mL, far superior to vancomycin and linezolid.

The present invention relates to an immunogenic construct with the capability to activate the mammalian immune system. In particular, the present invention relates to an immunogenic construct comprising bacterial proteins or fragments thereof that can both target and activate immune cells through interaction with pattern recognition receptors (PRR) expressed on the immune cells.

Aspects of the present disclosure relate to compositions and methods for treating one or more inflammatory bowel diseases ("IBD"), such as ulcerative colitis ("UC") and / or Crohn's disease. Some embodiments relate to a pharmaceutical dosage form comprising a core comprising an inhibitor of a protease (e.g., a bacterial protease) and a controlled release coating applied to an outer surface of the core. In some cases, the protease inhibitor is a gligliptin or a pharmaceutically acceptable salt thereof. In some cases, the controlled release coating is configured to release the protease inhibitor in the large intestine (e.g., colon) and / or small intestine of a subject administered the pharmaceutical dosage form. Some embodiments relate to methods of treating one or more IBDs comprising delivering a therapeutically effective amount of an inhibitor of a protease (e.g., bacterial protease) to the large intestine (e.g., colon) and / or the small intestine of a subject.

Synthetic bacterial messenger RNA can be used to make autologous, allogeneic or direct nucleic acid cancer vaccines. Cancer cells are transfected in vitro or in vivo with mRNA obtained from DNA encoding immunogenic bacterial proteins. The immune response to cancer is generated by direct administration of the mRNA in vivo or administration of a vaccine made from cancer cells in vitro. Codon modification of the mRNA can optimize expression of the immunogenic polypeptide in cancer cells.

This invention relates to an antibacterial membrane-penetrating peptide and its applications. The amino acid sequence of the antibacterial membrane-penetrating peptide includes the sequence shown in SEQ ID NO.1 or a sequence with more than 70% homology to the sequence shown in SEQ ID NO.1. This invention screened and obtained a short peptide that specifically binds to the bacterial Hsp70s protein. It was found that this peptide binds to the Hsp70s protein through a traditional substrate-binding pocket, exhibiting an unusually high affinity for the protein in the DnaK-ATP state. The short peptide can inhibit Hsp70s protein activity to a certain extent, preventing it from aiding in the renaturation of denatured luciferase. Furthermore, while maintaining the integrity of the bacterial cell membrane, this short peptide has a superior ability to cross the bacterial cell membrane. The invention further discusses its application potential in the preparation of antibacterial drugs and antibiotic adjuvants, providing new ideas for solving current problems such as bacterial resistance and the difficulty in discovering new antibiotics.

The invention relates to the technical field of fluorosilicone resin water cups. The fluorosilicon resin composite material is prepared from the following raw materials in parts by weight: 50 to 70 parts of hydroxyl-terminated polydimethylsiloxane, 10 to 20 parts of tetraethoxysilane, 25 to 35 parts of perdecafluorooctyltriethoxysilane, 5 to 15 parts of phenyltrimethoxysilane, 10 to 20 parts of xylene, 3 to 8 parts of an additive and 1 to 2 parts of dibutyltin dilaurate. According to the invention, ryegrass contains natural antibacterial ingredients of flavones and phenols, lignin and phenol in the powder also have antibacterial properties, phenolic hydroxyl groups of phenol can be combined with bacterial proteins to denature the proteins and inhibit bacterial growth, and benzyltriethylammonium chloride in the base material is added, so that bacteria can permeate to interfere the metabolism of the bacteria, and the antibacterial property of the powder is improved. Silver ions and quaternary ammonium salt can be slowly released through the resin matrix, the antibacterial effect is maintained for a long time, and the antibacterial performance of the fluorosilicone resin composite material water cup is improved.

The invention discloses the technical field of biological medicine, and particularly relates to application of caffeic acid phenethyl ester in preparation of a ribosome large subunit protein L1 targeted inhibitor. According to the present invention, the drug affinity induced target stability discovers that the ribosome large subunit protein L1 target inhibitor can directly target the ribosome large subunit protein L1 in bacteria, and can be adopted as the competitive inhibitor to competitively combine with the RRF L1 so as to inhibit the ribosome recovery process, such that the bacterial translation process is inhibited, and the bacterial protein synthesis blocking and death are caused. The mechanism enables the ribosome large subunit protein L1 to become a ribosome target with therapeutic potential, provides a new target and theoretical basis for clinical treatment of bacterial infection, and provides new support for screening of new broad-spectrum antibiotics.

This application discloses a demineralized water recycling system for a disc centrifuge, solving the technical problem of high cost in recovering bacterial protein. The disc centrifuge demineralized water recycling system includes a disc centrifuge with a demineralized water inlet pipe and a demineralized water outlet pipe; the inlet pipe of a circulating water tank is connected to the demineralized water outlet pipe, and the outlet pipe of the circulating water tank is connected to the demineralized water inlet pipe; a circulating pump is installed on the outlet pipe of the circulating water tank; the inlet pipe of a heating tank is also connected to the outlet pipe of the circulating water tank, and the inlet pipe of the heating water tank is equipped with a heating valve; a heating mechanism is installed inside the heating tank; the makeup water pipe of an alkali tank is connected to the outlet pipe of the heating tank; the makeup water pipe of an acid tank is connected to the outlet pipe of the heating tank; and the makeup water pipe of a hot water tank is connected to the outlet pipe of the heating tank. This application can reduce the cost of recovering bacterial protein using a disc centrifuge.

The application discloses a production process of a feed-grade high-protein D-calcium pantothenate particle, and the process comprises the following steps: (1) after inactivation of D-calcium pantothenate mash, ceramic filtrate and ceramic membrane residue liquid are obtained through membrane filtration; (2) after heating of the ceramic filtrate, wood active carbon is added for decolorization; decolorized liquid is obtained through suction filtration; (3) the decolorized liquid is mixed with part of the ceramic membrane residue liquid to prepare a mixed liquid with a dry basis purity of 52-58%; (4) after concentration of the mixed liquid to a dry solid content of 52-58%, auxiliary materials are added to prepare a dry basis purity of 42-48%, and a 42-48% D-calcium pantothenate particle product is obtained through granulation bed drying. The production process effectively reduces the production cost and environmental protection pressure, and meanwhile, the bacterial protein and auxiliary materials are used to prepare an animal-friendly feed D-calcium pantothenate product which is better in flowability and richer in nutrition.

The present invention relates to the field of bacterial protein engineering and protein fibers applicable as metal-binding bionanomaterials. More specifically, the present invention relates to engineered bacterial endospore appendage (Ena) proteins modified to contain metal-binding polypeptide (MBP) inserts providing for stable, flexible and robust protein assemblies with metal-binding activity. In particular, the invention relates to methods for designing Ena-fusion proteins capable of self-assembling into fibers, and for recombinant production of said self-assembling Ena-MBP fusion protein subunits, assemblies and fibers, ensuring a sustainable source of biological material for use in metal mineralization, metal sequestration, and metal-removal applications such as waste water treatment, water softening, or bioremediation.

The application discloses a method for producing feed by using glutamic acid isoelectric mother liquor, and belongs to the technical field of industrial fermentation. The technical scheme of the application comprises the following steps: first, the glutamic acid isoelectric mother liquor is subjected to ceramic membrane and ultrafiltration membrane treatment to obtain membrane filtrate and concentrated solution; the concentrated solution is subjected to plate and frame filtration and drying to obtain bacterial protein; the obtained membrane filtrate is subjected to electrodialysis treatment to obtain concentrated water and dilute water; the concentrated water is subjected to evaporation crystallization and solid-liquid separation to obtain ammonium sulfate crystals; and the dilute water is subjected to concentration and drying to obtain feed protein, so that the method for producing feed from the glutamic acid isoelectric mother liquor is realized. The treatment method can increase the yield of feed, improve the yield of ammonium sulfate and bacterial protein, and has great economic and environmental benefits.

The utility model provides a mycoprotein concentration system, and belongs to the field of mycoprotein preparation. The system comprises a raw material liquid tank; an inlet of the first-stage centrifugal machine is communicated with an outlet of the raw material liquid tank; an inlet of the first-stage concentrated liquid tank is communicated with a concentrated liquid outlet of the first-stage centrifugal machine; an inlet of the second-stage centrifugal machine is communicated with an outlet of the first-stage concentrated liquid tank, and a clear liquid outlet of the second-stage centrifugal machine is communicated with the raw material liquid tank; the second-stage concentrated liquid tank is communicated with a concentrated liquid outlet of the second-stage centrifugal machine; and an inlet of the drying tower is communicated with an outlet of the secondary concentrated liquid tank. Through continuous work of the first-stage centrifugal machine and the second-stage centrifugal machine, efficient concentration of mycoprotein residual distilled water is achieved, and the protein recovery rate is increased. Meanwhile, the clear liquid of the second-stage centrifugal machine contains higher mycoprotein, and the mycoprotein is discharged into the raw material liquid tank again to be centrifuged and utilized again, so that the recovery efficiency of the protein is further improved.

This invention relates to novel compositions, particularly compositions comprising one or more domains of bacterial Lon protease or related peptides. These compositions can be used to treat various diseases, such as infections, especially bacterial infections and cancer.

The invention discloses a Mycobacterium tuberculosis epitope mRNA (messenger Ribonucleic Acid) vaccine simulated, predicted and screened by a computer, which is prepared by the following steps: bacterial protein sequence acquisition and signal peptide analysis: acquiring amino acid sequences of proteins ESAT6, CFP10, Ag85A, Ag85B, TB10.4, PPE68, PPE18 and Rv1813c through a database; the method comprises the following steps: analyzing physicochemical properties of proteins, including the number of amino acids, the molecular weight, the instability index and the overall average value of water solubility, analyzing and predicting the allergy of the epitopes by using a server, and generating all potential mutants by using the server to predict and measure the toxicity of the epitopes; the multi-epitope mRNA vaccine candidate is successfully constructed by adopting computer simulation screening and optimization of the mycobacterium tuberculosis multi-epitope antigen, the vaccine design scheme is efficient, controllable and broad-spectrum, a theoretical basis and a candidate construct are provided for early research and development of tuberculosis vaccines, and the vaccine is expected to be used for prevention and control of tuberculosis and has positive influence on public health.

The invention relates to a positively charged peptide capable of non-covalently interact with negatively charged extracellular vesicles or nanoparticles. Said peptide is a fragment of the bacterial protein P40 and shows better interaction capabilities that the entire bacterial protein P40. The peptide of the invention can also be linked to a targeting element, such as targeting peptide, DNA aptamer, RNA aptamer, a protein, an antibody or a fragment thereof that binds to a target molecule, therefore it can be used for the targeted delivery of extracellular vesicles or nanoparticles further comprising a bioactive agent to a target cell, tissue or particle like a virus, that comprise the target molecule. Therefore, the present invention also relates to a carrier complex comprising the peptide of the invention non covalently bound to an extracellular vesicle or nanoparticle comprising a bioactive agent and its uses.

The application discloses a method for co-production of cellulase and bacterial protein based on an integrated microorganism-fiber microcarrier-surfactant suspension system, and belongs to the technical field of cellulase and bacterial protein production. First, the microorganism is inoculated on a culture medium to obtain a seed culture solution, then fiber microcarriers are prepared through micro-bubble continuous flotation, and then the fiber microcarriers are hydrolyzed by acid to form a fiber microcarrier hydrolysis mixed solution, which is dispersed in the microorganism and surfactant to prepare an integrated microorganism-fiber microcarrier-surfactant suspension system, microcarrier suspension fermentation is carried out, and after centrifugation and drying, the supernatant is cellulase, and the solid is bacterial protein. The method can effectively improve the yield of cellulase and bacterial protein, realize partial replacement of exogenous cellulase in the process of lignocellulose saccharification, and thus effectively reduce the preparation cost of lignocellulose saccharification.

The invention relates to the technical field of emission reduction and carbon sequestration of microorganisms, in particular to a carbon sequestration and alcohol production strain capable of improving the utilization rate of carbon atoms and a construction method and application of the carbon sequestration and alcohol production strain. Pyruvate formate lyase activating enzyme and / or formate dehydrogenase are / is overexpressed in the solvent-producing clostridium subjected to gene overexpression. The carbon-sequestration alcohol-producing strain capable of improving the utilization rate of the carbon atoms is used for producing alcohol solvents such as ethanol and butanol through anaerobic fermentation, the fermentation alcohol-producing concentration, the alcohol yield, the total carbon recovery rate of organic acid and solvent products and the content of mycoprotein and mycocarbon are all improved, the utilization rate of the carbon atoms is effectively improved, the economical efficiency of the carbon atoms is improved, and the production cost is reduced. And high-efficiency biological catalytic conversion of short-chain alcohol chemicals and energy products is realized.

The application discloses a kind of protein feed using potato residue solid fermentation and a preparation method thereof.Mainly with potato residue as main raw material, aspergillus niger, Candida tropicalis, Candida lipolytica and Saccharomyces cerevisiae are used as collaborative fermentation strains to carry out aerobic-anaerobic fermentation, and through single factor test and orthogonal test, the production process of solid fermentation bacterial protein feed using potato residue as raw material is studied.The bacterial protein feed process is produced by adopting composite strain solid fermentation, which can significantly increase the crude protein content and true protein content in fermentation product, improve the nutritional value, improve animal growth performance, and increase the shelf life and stability of protein feed, and also provides a new mode for non-grain type feed resources, especially potato residue waste resource utilization, effectively alleviates the plight of conventional feed resource shortage.

The utility model relates to the technical field of boards, and discloses a plasma antibacterial board which comprises a wood board base material, surface layer boards are arranged at the upper end and the lower end of the wood board base material, the surface layer boards are antibacterial boards processed through the plasma technology, the surface layer boards are hot-pressed on the wood board through high temperature and high pressure generated by ionized gas, and antibacterial factors are contained in the surface layer boards. After the antibacterial factors are catalyzed by high temperature and high pressure generated by ionized gas, quadruple antibacterial effective components including zinc ions, hydroxyl radicals, superoxide ions and hydrogen peroxide are formed. The antibacterial effective components can destroy the protein structure of bacteria and interfere the DNA synthesis of microorganisms, so that the efficient antibacterial effect is realized. The efficient and lasting antibacterial property is realized, and the bacterium breeding can be effectively inhibited.

The present invention relates to a method for developing genetically encoded fluorescent indicators based on orphan bacterial proteins. This approach uses a high-yield, unbiased method to identify orphan protein effectors or ligands. The technique is based on detecting variations in the spectroscopic signal that are due to the interaction of a test molecule with a protein, which is fused with one or two fluorescent proteins. The method can be implemented using purified proteins from systems such as bacteria, yeasts, insects or mammal cells, or using fusion proteins targeting the periplasm of Escherichia coli. The assessment is performed by optical system readers in 96- or 384-well formats, which allows for an efficient and scalable analysis. Once the specific ligand has been identified, the corresponding bacterial protein serves as a scaffold to design genetically encoded fluorescent indicators. The method incorporates a bioinformatic algorithm that facilitates the identification of orphan protein families based on genome or metagenome resources. These proteins are subsequently fused with fluorescent proteins and are assessed by comparing them with molecule libraries in order to determine their functionality. This approach allows the functional annotation of orphan proteins as transcription factors or periplasmic proteins, and their use in designing, developing and optimising fluorescent indicators. As a result, the ability to monitor emerging metabolites and metabolic networks with high precision and specificity is improved, in addition to facilitating the development of protocols to detect biomedically relevant molecules in biological fluids.

The present disclosure provides an engineered delivery system comprising bacterial membrane vesicles derived from bacteria and one or more non-bacterial proteins for anchoring to the bacterial membrane vesicles.

Synthetic bacterial messenger RNA can be used to make autologous, allogeneic or direct nucleic acid cancer vaccines. Cancer cells are transfected in vitro or in vivo with mRNA obtained from DNA encoding immunogenic bacterial proteins. The immune response to cancer is generated by direct administration of the mRNA in vivo or administration of a vaccine made from cancer cells in vitro. Codon modification of the mRNA can optimize expression of the immunogenic polypeptide in cancer cells.

The invention belongs to the field of medicinal chemistry, and discloses an aromatic pleuromutilin derivative with an antibacterial effect as well as a preparation method and application of the aromatic pleuromutilin derivative. The pleuromutilin is structurally modified, aromatic group modification is further increased through a reductive amination method after mercaptoethylamine is introduced into a C-14 side chain, and the structural formula of the pleuromutilin is shown in the specification. Research on an antibacterial mechanism finds that the series of derivatives can effectively interfere with expression of bacterial protein and show unique and excellent antibacterial activity, and the pleuromutilin can be applied to preparation of antibacterial agents. Compared with pleuromutilin in the prior art, most of the pleuromutilin compounds have antibacterial activity on staphylococcus aureus, escherichia coli, salmonella, micrococcus luteus and enterococcus faecalis which are common in in-vitro clinical application, hardly generate drug resistance, have stable safety and are expected to be further optimized into lead compounds of novel antibiotics.

The invention provides a pyridine oxazolidinone derivative or a pharmaceutical salt thereof as well as a preparation method and application of the pyridine oxazolidinone derivative, and belongs to the technical field of medicine synthesis. The pyridine oxazolidinone derivative and the pharmaceutically acceptable salt thereof provided by the invention have excellent antibacterial activity, inhibit formation of a 70S initial compound by targeting 23S rRNA of a bacterial ribosome 50S subunit, enable synthesis of bacterial protein to be retarded in an initial stage and further play an antibacterial effect, do not have the problem of cross resistance with other existing antibiotics, and have good application prospects. The compound can be used for preparing anti-gram-positive bacterium drugs. The preparation method of the pyridine oxazolidinone derivative and the medicinal salt of the pyridine oxazolidinone derivative is simple in step, convenient to operate and high in feasibility.

The invention relates to chitophage protein glutaminase PG5, a coding gene and application of the chitophage protein glutaminase PG5, and belongs to the technical field of biological genetic engineering. The amino acid sequence of the protein glutaminase PG5 is as shown in SEQ ID No. 1, and the cDNA sequence of the coding gene of the protein glutaminase PG5 is as shown in SEQ ID No. 2. A large amount of chitophage-derived protein glutaminase PG5 is prepared through an escherichia coli expression system, and the protein glutaminase PG5 is used for carrying out enzymatic deamidation modification on the myofibrillar protein of the spotted silver carp, so that the thermal reversible protein gel is successfully prepared in a salt-free system. An important tool enzyme is provided for protein deamidation modification, unprecedented flexibility is provided for processing adaptability and cold-chain storage and transportation of fish protein gel products, and the application range of the fish protein gel products in food design is greatly expanded.

The utility model discloses a mash concentration device and a mycoprotein production line, and belongs to the technical field of mycoprotein production. The mash concentration device comprises an evaporation tank and a heat exchange tube. The evaporation tank is provided with an evaporation cavity, and a water vapor outlet, a mash inlet and a mash outlet which are communicated with the evaporation cavity, and the mash inlet is used for being communicated with mash. The heat exchange pipe is provided with a heat exchange pipe inlet and a heat exchange pipe outlet which are communicated, the heat exchange pipe inlet and the heat exchange pipe outlet are both located outside the evaporation cavity, the heat exchange pipe inlet is used for being communicated with a steam exhaust port of the oxidation furnace, and at least part of the heat exchange pipe is located in the evaporation cavity. According to the mash concentration device, the steam generated by the oxidation furnace is used as a heat source to concentrate the mash, so that the steam generated by the oxidation furnace can also be used by the mash concentration device, and the utilization rate of the steam generated by the oxidation furnace is improved.

The invention relates to the technical field of soybean meal fermentation, and discloses a soybean meal bacillus mixed fermentation enzyme production method, which comprises the following steps: S1, strain selection; s2, high-density fermentation; s3, preparing a complex microbial inoculant; s4, preparation and preservation; s5, crushing the soybean meal substrate; s6, pretreating a soybean meal substrate; s7, performing sterilization; s8, soybean meal fermentation; s9, extracting an enzyme preparation; s10, drying the fermented soybean meal; according to the scheme, three substrates, namely protein, starch and cellulose, are efficiently and specially attacked by a plurality of strains respectively, the degradation bottleneck problem caused by a single enzyme system or an incomplete enzyme system is solved, so that macromolecular substances of the soybean meal are decomposed more thoroughly and quickly, and residues after enzyme are extracted by adopting a two-stage countercurrent extraction technology, so that the yield of the soybean meal is improved. The probiotic feed additive is rich in mycoprotein, small peptide and incompletely degraded fiber, so that the probiotic feed additive with high quality can be prepared after simple drying, and resource utilization of by-products is realized.

Methods for the detection of subjects at risk of developing rheumatoid arthritis, and subjects having early rheumatoid arthritis thus allowing for early intervention. Compositions for inducing immune tolerance in a subject thus treating or slowing progression of RA. Kits useful in the diagnosis of RA.

This invention discloses a protein-induced self-growing transparent hydrogel antifouling coating and its preparation method, belonging to the field of marine antifouling gel coatings. The preparation method involves culturing bacteria that secrete large amounts of extracellular polymers and obtaining a sterile supernatant; adding a protein precipitant to obtain a precipitate; adding a protein precipitation washing reagent and mixing thoroughly to obtain the target protein, which is then freeze-dried and purified; immersing a substrate sample in a bacterial protein solution to deposit a protein coating; preparing a hydrogel precursor solution; immersing the protein coating in the hydrogel precursor solution; and allowing it to grow statically to obtain a protein-induced self-growing transparent hydrogel antifouling coating. This invention overcomes the shortcomings of current hydrogels, such as poor adhesion to the substrate and high cost, and successfully prepares a robust, low-cost, and environmentally friendly hydrogel adhesion layer with in-situ initiation and subsequent self-growth. It is not limited by traditional environmental constraints such as light and heat, thus expanding the application scenarios of hydrogels.