94 results about "Bacterial protein" patented technology

Compositions and methods for treating or reducing the severity of occurrence of a parasitic worm or helminth infection in a subject are described. The methods include administering to the subject a therapeutically effective amount of a composition comprising isolated native, bioactive nematicidal crystals formed from a single type of nematicidal crystal protein. The isolated native, bioactive nematicidal crystals are substantially free of any bacterial spores or host bacterial proteins, other than nematicidal crystal protein in the form of a crystal. Methods for making isolated native, bioactive nematicidal crystals are also described. The crystal proteins may be full length, truncated, variant, or sub-variant Cry proteins. Examples of crystal proteins include Cry5B, Cry21, Cry14A, Cry6A, and Cry13A.

Compositions and methods for treating or reducing the severity of occurrence of a parasitic worm or helminth infection in a subject are described. The methods include administering to the subject a therapeutically effective amount of a composition comprising isolated native, bioactive nematicidal crystals formed from a single type of nematicidal crystal protein. The isolated native, bioactive nematicidal crystals are substantially free of any bacterial spores or host bacterial proteins, other than nematicidal crystal protein in the form of a crystal. Methods for making isolated native, bioactive nematicidal crystals are also described. The crystal proteins may be full length, truncated, variant, or sub-variant Cry proteins. Examples of crystal proteins include Cry5B, Cry21, Cry14A, Cry6A, and Cry13A.

The invention relates to a protein feed prepared from biologically refined distiller's grains and a preparation method thereof, and relates to the technical field of bioengineering and feed resource development. The method comprises the following steps: (1) sequentially carrying out acid pretreatment and double-enzyme synergistic hydrolysis on the white spirit vinasse to obtain hydrolysate containing fermentable sugar; (2) adding a candida utilis yeast liquid and urea, and carrying out carbon-nitrogen synergistic liquid fermentation to obtain a fermentation liquid with the viable count of candida utilis greater than or equal to 4.0 * 10 < 8 > CFU / mL; and drying. According to the method, the white spirit vinasse is subjected to diluted acid high-pressure pretreatment and double-enzyme synergistic hydrolysis, then candida utilis is utilized for carbon-nitrogen synergistic liquid fermentation, efficient utilization of glucose and xylose is achieved, and the mycoprotein yield is larger than or equal to 0.70 g / L / h; according to the method, all-component high-value utilization of the distiller's grains is realized, and a process path with industrial production potential is provided for preparing the protein feed by biorefining the distiller's grains.

The invention discloses a preparation method of a fructus cannabis protein peptide product with effects of resisting fatigue and improving exercise ability, which comprises the following steps: performing preliminary crushing, ultralow-temperature freezing micro-crushing and low-temperature continuous phase-change extraction degreasing on fructus cannabis, performing step-by-step hydrolysis by using polysaccharase and 2.4 L bacterial protease to extract fructus cannabis protein, performing low-temperature salting-out, and performing freeze-drying to obtain the fructus cannabis protein peptide product with the effects of resisting fatigue and improving exercise ability. The fructus cannabis protein peptide powder is prepared through low-temperature ultrasonic dissolution promotion, high-pressure micro-jet homogenization, compound enzyme C hydrolysis, 1% pepsase, high-temperature enzyme deactivation, chlorophyll removal through counter-current extraction, hydrogen peroxide decoloration, ultralow-temperature instantaneous concentration, low-temperature vacuum belt type drying and low-temperature ultrahigh-pressure sterilization. The fructus cannabis protein peptide prepared by the invention has the following remarkable effects: 1, the exercise ability is remarkably improved, the accumulation of lactic acid and urea nitrogen is reduced, the exhaustion time is prolonged, and the physical and mental fatigue is relieved; 2, the content of hepatic glycogen and muscle glycogen is obviously increased, the energy reserve of the body is increased, the explosive power and the reactivity are improved, and the athletic performance is improved; and 3, the antioxidant level of the body is improved, and fatigue and immune recovery after exercise are promoted.

The invention relates to the technical field of plasmalogen, and discloses a preparation method and application of an amide plasmalogen compound, and the preparation method comprises the following steps: (1) reacting a compound 1 and a compound 2 in an HEPES buffer solution to obtain a compound 3; (2) reacting the compound 3 in an ethyl acetate phase and a water phase by using phospholipase D under the assistance of Ca < 2 + > ions to obtain a compound 4; (3) expression and purification of CarF protein from myxobacteria; and (4) reacting the compound 4 and CarF protein in a Tris-HCl buffer solution under the assistance of NADH (Nicotinamide Adenine Dinucleotide) and catalase to obtain a compound 5. According to the invention, the purified recombinant myxobacteria CarF protein and the chemically synthesized CarF protein reaction substrate are adopted to finally synthesize the amide plasmalogen, and the amide plasmalogen can show the effect similar to that of natural plasmalogen.

The invention relates to the technical field of environmental microorganisms and resource fermentation, and discloses a Kluyveromyces marxianus BMK7 for protein production and biogas slurry purification and application of the Kluyveromyces marxianus BMK7, the BMK7 is Kluyveromyces marxianus and is obtained through ultraviolet mutagenesis and hygromycin B adaptive evolution, the preservation number is CCTCC NO: M 20251050, and the Kluyveromyces marxianus BMK7 has good growth performance and genetic stability. The strain can be efficiently fermented under the condition that biogas slurry serves as a main substrate, ammonia nitrogen and chemical oxygen demand (COD) in the biogas slurry are remarkably reduced, and meanwhile high-proportion mycoprotein is synthesized. The thalli have strong flocculability and good sedimentation characteristics, and are beneficial to protein recovery. The method can be widely applied to treatment and resource utilization of sewage such as breeding wastewater, kitchen fermentation liquor and anaerobic effluent, and has a good industrialization prospect.

The invention relates to a silica gel material and a preparation method thereof, and belongs to the technical field of high polymer materials. The silica gel material comprises the following components in parts by weight: 80-120 parts of vinyl silica gel, 10-25 parts of vinyl silicone oil, 15-25 parts of reinforcing filler, 3-6 parts of a modified antibacterial agent, 1-3 parts of a modified flexibilizer, 2-6 parts of a foaming agent and 2-6 parts of a cross-linking agent, a quaternary ammonium salt pre-product in the modified antibacterial agent permeates bacterial cell walls, reacts with proteins of cell membranes and adsorbs bacteria through electrostatic interaction, carbazole is combined with anions on the surfaces of the bacteria to destroy bacterial proteins, and cinnamic acid inhibits protein synthesis; a maleimide group in the modified toughening agent enhances the flexibility of a molecular chain, improves the toughness of the material, also improves the glass transition temperature and the melting point of the material, and prevents the material from embrittlement in a high-temperature environment, and polyurethane enhances the toughness of the material; the silica gel material prepared by the invention not only has a good antibacterial effect, but also has excellent heat resistance and toughening performance.

This invention relates to a 1,3,4-thiadiazole peptide deformylase inhibitor and its preparation and application. The structural formula is shown in formula (1): wherein, R 1 It is n-butyl or cyclopentylmethyl; R 2 The compounds are hydrogen-containing, straight-chain alkanes, cyclic alkanes, aromatic rings, substituted biphenyls, or heterocyclic rings. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention can effectively inhibit bacterial protein synthesis, thereby achieving sterilization. These compounds exhibit excellent inhibitory activity against Gram-positive drug-resistant bacteria, especially the clinically challenging methicillin-resistant Staphylococcus aureus (MRSA). In particular, some preferred compounds show inhibitory activity 4-8 times that of the control standards vancomycin and linezolid. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention also exhibit inhibitory activity against Gram-negative drug-resistant bacteria, especially against drug-resistant Acinetobacter baumannii, known as "superbugs," with an inhibitory activity reaching 0.5 μg / mL, far superior to vancomycin and linezolid.

The present invention relates to an immunogenic construct with the capability to activate the mammalian immune system. In particular, the present invention relates to an immunogenic construct comprising bacterial proteins or fragments thereof that can both target and activate immune cells through interaction with pattern recognition receptors (PRR) expressed on the immune cells.

The application discloses a preparation method of glutamic acid bacterial protein ecological fertilizer, and the preparation steps are as follows: firstly, zinc polysilicate is added into glutamic acid fermentation waste liquid, and bacterial protein-zinc polysilicate complex is obtained through filtration; then, zinc silicate and hydroxyapatite are added, and amino acid composite hydroxyapatite particles are formed through the action of citric acid; and finally, urea-humic acid-dipotassium hydrogen phosphate complex is sprayed on the amino acid composite hydroxyapatite particles to obtain the ecological fertilizer. The ecological fertilizer can not only fully utilize the bacterial protein in the glutamic acid fermentation waste liquid, but also play a good slow-release effect of nitrogen, phosphorus and other elements through the action of two complexes. In addition, the addition of silicon and zinc elements can play a supplementing effect for the growth of crops, so that the fertilizer has the dual functions of environmental protection and long-acting slow release, and has a good promoting effect on the growth of crops.

Aspects of the present disclosure relate to compositions and methods for treating one or more inflammatory bowel diseases ("IBD"), such as ulcerative colitis ("UC") and / or Crohn's disease. Some embodiments relate to a pharmaceutical dosage form comprising a core comprising an inhibitor of a protease (e.g., a bacterial protease) and a controlled release coating applied to an outer surface of the core. In some cases, the protease inhibitor is a gligliptin or a pharmaceutically acceptable salt thereof. In some cases, the controlled release coating is configured to release the protease inhibitor in the large intestine (e.g., colon) and / or small intestine of a subject administered the pharmaceutical dosage form. Some embodiments relate to methods of treating one or more IBDs comprising delivering a therapeutically effective amount of an inhibitor of a protease (e.g., bacterial protease) to the large intestine (e.g., colon) and / or the small intestine of a subject.

Synthetic bacterial messenger RNA can be used to make autologous, allogeneic or direct nucleic acid cancer vaccines. Cancer cells are transfected in vitro or in vivo with mRNA obtained from DNA encoding immunogenic bacterial proteins. The immune response to cancer is generated by direct administration of the mRNA in vivo or administration of a vaccine made from cancer cells in vitro. Codon modification of the mRNA can optimize expression of the immunogenic polypeptide in cancer cells.

This invention relates to an antibacterial membrane-penetrating peptide and its applications. The amino acid sequence of the antibacterial membrane-penetrating peptide includes the sequence shown in SEQ ID NO.1 or a sequence with more than 70% homology to the sequence shown in SEQ ID NO.1. This invention screened and obtained a short peptide that specifically binds to the bacterial Hsp70s protein. It was found that this peptide binds to the Hsp70s protein through a traditional substrate-binding pocket, exhibiting an unusually high affinity for the protein in the DnaK-ATP state. The short peptide can inhibit Hsp70s protein activity to a certain extent, preventing it from aiding in the renaturation of denatured luciferase. Furthermore, while maintaining the integrity of the bacterial cell membrane, this short peptide has a superior ability to cross the bacterial cell membrane. The invention further discusses its application potential in the preparation of antibacterial drugs and antibiotic adjuvants, providing new ideas for solving current problems such as bacterial resistance and the difficulty in discovering new antibiotics.

The invention relates to the technical field of biology, and particularly discloses marine bacterial protease as well as a coding gene, a preparation method and application thereof. The invention provides protease 4SP5-519 capable of specifically degrading allergenic tropomyosin in aquatic products and reducing the sensitization of the allergenic tropomyosin, and the protease 4SP5-519 is applied to improving the food safety of the aquatic products.

The invention relates to the technical field of fluorosilicone resin water cups. The fluorosilicon resin composite material is prepared from the following raw materials in parts by weight: 50 to 70 parts of hydroxyl-terminated polydimethylsiloxane, 10 to 20 parts of tetraethoxysilane, 25 to 35 parts of perdecafluorooctyltriethoxysilane, 5 to 15 parts of phenyltrimethoxysilane, 10 to 20 parts of xylene, 3 to 8 parts of an additive and 1 to 2 parts of dibutyltin dilaurate. According to the invention, ryegrass contains natural antibacterial ingredients of flavones and phenols, lignin and phenol in the powder also have antibacterial properties, phenolic hydroxyl groups of phenol can be combined with bacterial proteins to denature the proteins and inhibit bacterial growth, and benzyltriethylammonium chloride in the base material is added, so that bacteria can permeate to interfere the metabolism of the bacteria, and the antibacterial property of the powder is improved. Silver ions and quaternary ammonium salt can be slowly released through the resin matrix, the antibacterial effect is maintained for a long time, and the antibacterial performance of the fluorosilicone resin composite material water cup is improved.

The invention discloses the technical field of biological medicine, and particularly relates to application of caffeic acid phenethyl ester in preparation of a ribosome large subunit protein L1 targeted inhibitor. According to the present invention, the drug affinity induced target stability discovers that the ribosome large subunit protein L1 target inhibitor can directly target the ribosome large subunit protein L1 in bacteria, and can be adopted as the competitive inhibitor to competitively combine with the RRF L1 so as to inhibit the ribosome recovery process, such that the bacterial translation process is inhibited, and the bacterial protein synthesis blocking and death are caused. The mechanism enables the ribosome large subunit protein L1 to become a ribosome target with therapeutic potential, provides a new target and theoretical basis for clinical treatment of bacterial infection, and provides new support for screening of new broad-spectrum antibiotics.

The present invention relates to a preparation method of an exocrine protein Pc and its application in regulating intestinal flora. The fungal-derived Pc protein directly interacts with a protein in the intestinal microorganisms that has exocrine properties and contains a β-N-acetylglucosaminidase (GL) domain and inhibits its enzymatic activity, thereby regulating the proliferation of beneficial microorganisms by alleviating cellular iron and ROS pressure. The Pc protein can regulate the expression of bifunctional autolysins, and can enrich beneficial Staphylococcus bacteria with bifunctional autolysins in the insect intestine, or can enrich beneficial Lachnospiraceae bacteria with bifunctional autolysins in the intestines of healthy mammalian mice, or can enrich Blautia bacteria in the intestines of obese mice induced by a high-fat diet, or can enrich Akkermansia bacteria in the intestines of mice with DSS-induced colitis. The Pc protein can significantly reduce the formation of biofilms of Staphylococcus aureus (S. aureus) with bifunctional autolysins. Pc protein can be used as a new potential prebiotic to regulate the proliferation of beneficial intestinal microorganisms with bifunctional autolysins, and has potential application value in the preparation of food, medicine and disease treatment.

This invention relates to the field of animal husbandry technology, specifically a processing method for improving the digestibility of rice husk nutrients in rabbits. The method includes steps 1) micronizing rice husks to obtain rice husk powder; 2) acidifying the rice husk powder; and 3) puffing the material obtained in step 2). This method can alter the fiber composition of rice husk powder, reducing crude fiber content, especially lignin content, and increasing nitrogen-free extract content. It helps acidify the rabbit's intestines, increases intestinal nitrogen sources to promote bacterial protein synthesis, and improves the digestibility of rice husk powder nutrients in rabbits.

The present disclosure provides host cells for reliable, high yield recombinant protein production, including unstable proteins. The present host cell (e.g., a bacterial cell) is deficient in at least one protease (or a subunit of a protease) such as Clp or ClpP. The host cell may also contain an expression vector that encodes a protein or polypeptide for overexpression.

This application discloses a demineralized water recycling system for a disc centrifuge, solving the technical problem of high cost in recovering bacterial protein. The disc centrifuge demineralized water recycling system includes a disc centrifuge with a demineralized water inlet pipe and a demineralized water outlet pipe; the inlet pipe of a circulating water tank is connected to the demineralized water outlet pipe, and the outlet pipe of the circulating water tank is connected to the demineralized water inlet pipe; a circulating pump is installed on the outlet pipe of the circulating water tank; the inlet pipe of a heating tank is also connected to the outlet pipe of the circulating water tank, and the inlet pipe of the heating water tank is equipped with a heating valve; a heating mechanism is installed inside the heating tank; the makeup water pipe of an alkali tank is connected to the outlet pipe of the heating tank; the makeup water pipe of an acid tank is connected to the outlet pipe of the heating tank; and the makeup water pipe of a hot water tank is connected to the outlet pipe of the heating tank. This application can reduce the cost of recovering bacterial protein using a disc centrifuge.

The application discloses a production process of a feed-grade high-protein D-calcium pantothenate particle, and the process comprises the following steps: (1) after inactivation of D-calcium pantothenate mash, ceramic filtrate and ceramic membrane residue liquid are obtained through membrane filtration; (2) after heating of the ceramic filtrate, wood active carbon is added for decolorization; decolorized liquid is obtained through suction filtration; (3) the decolorized liquid is mixed with part of the ceramic membrane residue liquid to prepare a mixed liquid with a dry basis purity of 52-58%; (4) after concentration of the mixed liquid to a dry solid content of 52-58%, auxiliary materials are added to prepare a dry basis purity of 42-48%, and a 42-48% D-calcium pantothenate particle product is obtained through granulation bed drying. The production process effectively reduces the production cost and environmental protection pressure, and meanwhile, the bacterial protein and auxiliary materials are used to prepare an animal-friendly feed D-calcium pantothenate product which is better in flowability and richer in nutrition.

The present invention relates to the field of bacterial protein engineering and protein fibers applicable as metal-binding bionanomaterials. More specifically, the present invention relates to engineered bacterial endospore appendage (Ena) proteins modified to contain metal-binding polypeptide (MBP) inserts providing for stable, flexible and robust protein assemblies with metal-binding activity. In particular, the invention relates to methods for designing Ena-fusion proteins capable of self-assembling into fibers, and for recombinant production of said self-assembling Ena-MBP fusion protein subunits, assemblies and fibers, ensuring a sustainable source of biological material for use in metal mineralization, metal sequestration, and metal-removal applications such as waste water treatment, water softening, or bioremediation.

The application discloses a method for producing feed by using glutamic acid isoelectric mother liquor, and belongs to the technical field of industrial fermentation. The technical scheme of the application comprises the following steps: first, the glutamic acid isoelectric mother liquor is subjected to ceramic membrane and ultrafiltration membrane treatment to obtain membrane filtrate and concentrated solution; the concentrated solution is subjected to plate and frame filtration and drying to obtain bacterial protein; the obtained membrane filtrate is subjected to electrodialysis treatment to obtain concentrated water and dilute water; the concentrated water is subjected to evaporation crystallization and solid-liquid separation to obtain ammonium sulfate crystals; and the dilute water is subjected to concentration and drying to obtain feed protein, so that the method for producing feed from the glutamic acid isoelectric mother liquor is realized. The treatment method can increase the yield of feed, improve the yield of ammonium sulfate and bacterial protein, and has great economic and environmental benefits.

Provided herein are compositions, systems, and methods for the assembly of a positioning system for protein-based organelles within a cell of an organism. In particular, the present invention relates to systems of homeostasis for both synthetic and natural bacterial protein-based organelles including bacterial microcompartments, encapsulin nanocompartments, and biomolecular condensates.

The utility model provides a mycoprotein concentration system, and belongs to the field of mycoprotein preparation. The system comprises a raw material liquid tank; an inlet of the first-stage centrifugal machine is communicated with an outlet of the raw material liquid tank; an inlet of the first-stage concentrated liquid tank is communicated with a concentrated liquid outlet of the first-stage centrifugal machine; an inlet of the second-stage centrifugal machine is communicated with an outlet of the first-stage concentrated liquid tank, and a clear liquid outlet of the second-stage centrifugal machine is communicated with the raw material liquid tank; the second-stage concentrated liquid tank is communicated with a concentrated liquid outlet of the second-stage centrifugal machine; and an inlet of the drying tower is communicated with an outlet of the secondary concentrated liquid tank. Through continuous work of the first-stage centrifugal machine and the second-stage centrifugal machine, efficient concentration of mycoprotein residual distilled water is achieved, and the protein recovery rate is increased. Meanwhile, the clear liquid of the second-stage centrifugal machine contains higher mycoprotein, and the mycoprotein is discharged into the raw material liquid tank again to be centrifuged and utilized again, so that the recovery efficiency of the protein is further improved.

This invention relates to novel compositions, particularly compositions comprising one or more domains of bacterial Lon protease or related peptides. These compositions can be used to treat various diseases, such as infections, especially bacterial infections and cancer.

The present invention relates to a conjugate comprising a ligand specifically binding to a bacterial lectin, a linker comprising a peptide cleavable by a bacterial protease, and an anti-bacterial therapeutic agent or imaging agent. It further relates to the conjugate or the pharmaceutical composition or diagnostic composition for use in medicine.

The invention discloses a Mycobacterium tuberculosis epitope mRNA (messenger Ribonucleic Acid) vaccine simulated, predicted and screened by a computer, which is prepared by the following steps: bacterial protein sequence acquisition and signal peptide analysis: acquiring amino acid sequences of proteins ESAT6, CFP10, Ag85A, Ag85B, TB10.4, PPE68, PPE18 and Rv1813c through a database; the method comprises the following steps: analyzing physicochemical properties of proteins, including the number of amino acids, the molecular weight, the instability index and the overall average value of water solubility, analyzing and predicting the allergy of the epitopes by using a server, and generating all potential mutants by using the server to predict and measure the toxicity of the epitopes; the multi-epitope mRNA vaccine candidate is successfully constructed by adopting computer simulation screening and optimization of the mycobacterium tuberculosis multi-epitope antigen, the vaccine design scheme is efficient, controllable and broad-spectrum, a theoretical basis and a candidate construct are provided for early research and development of tuberculosis vaccines, and the vaccine is expected to be used for prevention and control of tuberculosis and has positive influence on public health.

The present invention relates to the field of genetic engineering technology, and particularly to the application of BTR1 protein in improving the disease resistance of plants or cultivating transgenic plants with disease resistance. The present invention provides the application of BTR1 protein in improving the disease resistance of plants or cultivating transgenic plants with disease resistance. The amino acid sequence of the BTR1 protein is as shown in SEQ ID NO.2, and the pathogens of the disease resistance include one or more of oomycetes, bacteria and viruses. The present invention introduces the bacterial BTR1 protein into plant disease resistance and discovers its role in plant disease resistance. Expressing the BTR1 protein in plants can improve the resistance of plants to oomycetes, bacteria and viruses, achieve combined disease resistance, and help plants achieve disease-resistant cultivation and breeding. The present invention expands the screening range of plant R genes and effectively reduces the time and effort of traditional R gene screening methods.

The invention relates to a positively charged peptide capable of non-covalently interact with negatively charged extracellular vesicles or nanoparticles. Said peptide is a fragment of the bacterial protein P40 and shows better interaction capabilities that the entire bacterial protein P40. The peptide of the invention can also be linked to a targeting element, such as targeting peptide, DNA aptamer, RNA aptamer, a protein, an antibody or a fragment thereof that binds to a target molecule, therefore it can be used for the targeted delivery of extracellular vesicles or nanoparticles further comprising a bioactive agent to a target cell, tissue or particle like a virus, that comprise the target molecule. Therefore, the present invention also relates to a carrier complex comprising the peptide of the invention non covalently bound to an extracellular vesicle or nanoparticle comprising a bioactive agent and its uses.