14 results about "Myxobacteria" patented technology

The invention belongs to the technical field of biology and new medicine, and discloses a method for constructing a myxobacteria expression chassis with genetic stability and application of the myxobacteria expression chassis. Through gene deletion and overexpression analysis, it is verified that the spontaneous mutation rate of a strain can be reduced by deletion of a TLS pathway and overexpression of RecA, and a myxobacteria strain 2K7 with genetic stability is further constructed on the basis. The spontaneous mutation rate of the new strain is reduced by 11.5 times, and the transformation capability of growth plasmids of the strain is obviously improved. According to the research, the genetic stability of the myxobacteria is improved for the first time through a repair way after modification and replication, and the research method and the research result are innovative.

The invention belongs to the field of microbiology and molecular biology, and particularly relates to a method for extracting myxococcus flavus dormant mucospore RNA (Ribonucleic Acid). The method specifically comprises the following steps: taking a certain amount of myxococcus flavus liquid, and inducing sporocarp development by using a common starvation induction method; scraping the formed sporocarp structure, resuspending with sterile water, and destroying the sporocarp structure and mucospore coating by using a certain proportion of organic reagent; centrifuging, taking a water phase, adding an acetate buffer solution with a certain concentration, and precipitating with isopropanol to obtain nucleic acid substances; and removing DNA by using a conventional kit to obtain RNA. Aiming at the characteristic that the mucospore RNA is difficult to extract, the invention develops a simple, convenient and efficient method for extracting the mucospore RNA, and provides an effective way for deeply researching the ecological characteristics of the myxobacteria.

The application discloses a gene prdR for regulating myxobacteria predation and a protein coded by the gene, and belongs to the technical field of biotechnology. The mutant DK10 with significantly improved predation capacity is obtained by using the strategy of adaptive evolution; genetic variation sites of the mutant DK10 are analyzed by using the bacterial genome resequencing technology, and functions of the genetic variation sites are researched by using the gene knockout technology, and it is found that the gene deletion of MXAN_2902 (prdR, the nucleotide sequence is shown as SEQ ID NO. 1) significantly enhances the predation capacity of Myxococcus xanthus. The nucleic acid sequence and the amino acid sequence of the transcriptional activator PrdR for regulating the predation of Myxococcus xanthus are disclosed, and the functional strain of Myxococcus xanthus capable of efficiently predating Salmonella enteritidis can be developed by using the prdR gene, and a new strategy is provided for the prevention and control of drug-resistant HVPG pathogenic bacteria.

The invention discloses a method for efficient traceless editing of a myxobacteria genome and application. According to the invention, a TnpB cutting system ISDra2 of deinococcus radiodurans is combined with a homologous recombination system MxRedET of myxobacteria, so that a one-step traceless editing platform (MxDIRECT) is formed. The ISDra2 is responsible for accurately positioning and cutting target DNA, and the MxRedET immediately realizes efficient fragment integration or replacement at a cut, so that operations such as gene knockout, insertion and replacement are realized. According to the method, the editing efficiency of myxobacteria is improved to 100% from original 1%, the period is shortened to 5-7 days from 3-6 months, and one-step double-site traceless editing can be achieved through double-site design. The longest DNA of about 80 kb can be knocked out through a single incision, and the longest DNA can reach 200 kb through double incisions; and mononucleotide replacement can be carried out at any position, so that accurate base editing is realized. The technology provides an efficient and accurate tool for modifying functional genes and metabolic pathways of the myxobacteria chassis cells, and obviously promotes the research of synthetic biology.

The invention discloses an application of a polypeptide in inhibiting bacteria or preparing a reagent for inhibiting bacteria, and belongs to the technical field of biology. According to preferred codons of bacillus subtilis, a polypeptide coding gene which is derived from myxobacteria and has unknown functions is designed, and a nucleotide sequence is obtained. And then carrying out recombinant expression in bacillus subtilis by utilizing a genetic engineering technology. The fermentation liquor of the recombinant bacillus subtilis shows better antibacterial activity on gram-positive bacteria represented by staphylococcus aureus, pseudomonas aeruginosa, salmonella, escherichia coli and gram-negative bacteria represented by vibrio parahaemolyticus. The antibacterial function of the polypeptide in myxobacteria is found for the first time, namely, the novel antibacterial peptide is provided, and compared with traditional antibiotics, the novel antibacterial peptide is equivalent or higher in antibacterial activity, wide in antibacterial spectrum and low in preparation cost and has wide application prospects in the fields of antibacterial preparations, food preservatives and the like.

This invention belongs to the fields of microbiology and molecular biology, specifically relating to a homologous recombinase combination in myxobacteria and its application in gene editing. Specifically, this invention provides four pairs of homologous recombinase systems capable of efficiently mediating in vivo recombination in myxobacteria. Each pair includes a protein YqaJ with exonuclease function and a protein RecT with single-strand annealing function. This invention provides a method for myxobacterial gene editing: under the action of homologous recombinase pairs, mutant strains are obtained through positive screening using resistance tags. The resistance tags are then successfully removed through the combined action of the Cre / loxP system and the reverse screening gene galK. This method achieves a gene editing efficiency of up to 100%, has a short cycle time, requires no purification, and can be used for iterative deletion of multiple genes to achieve genome simplification in myxobacteria, or for inserting functional promoters and other elements to activate silent gene clusters. This method is of great significance for the bioexploration and functional genomics research of myxobacteria.

The application discloses application of a polypeptide in inhibiting bacteria or preparing a bacterium-inhibiting agent and belongs to the technical field of biotechnology. In the application, a polypeptide coding gene of unknown function from myxobacteria is designed according to the preferred codon of bacillus subtilis, and a nucleotide sequence is obtained. Then, the gene engineering technology is used to make the polypeptide coding gene recombinantly expressed in bacillus subtilis. The fermentation liquor of the recombinant bacillus subtilis shows good antibacterial activity on gram-positive bacteria represented by staphylococcus aureus, gram-negative bacteria represented by pseudomonas aeruginosa, salmonella, escherichia coli and vibrio parahaemolyticus. The application first discovers the antibacterial function of the polypeptide in myxobacteria, that is, a novel antibacterial peptide is provided. Compared with traditional antibiotics, the antibacterial peptide has equivalent or higher antibacterial activity, a wide antibacterial spectrum and low preparation cost, and has wide application prospects in the fields of antibacterial preparations, food preservatives and the like.

The invention relates to a pipeline layout optimization method and device based on big data, equipment and a medium. According to the method, multi-source heterogeneous data is collected, a standardized data set is constructed, and a three-dimensional digital environment model is established based on the data set; defining a pipe network source sink in the model and initializing a myxobacteria network to form an initial topological structure, and simulating biological behavior characteristics of myxobacteria to carry out iterative optimization on the initial structure; engineering processing is carried out on the optimized topological structure to meet actual engineering constraints, and finally an optimal layout scheme is selected and output through multi-target evaluation. According to the method, a real environment model is constructed by using big data to serve as a growth culture medium of a myxobacteria algorithm, a pipe network topology is generated under complex constraints through a myxobacteria self-organized bionic optimization mechanism, and then a layout scheme capable of being directly implemented is formed through engineering transformation, so that full-process optimization from environment perception and self-adaptive growth to engineering landing is realized, and the method is suitable for large-scale popularization and application. And the global optimality, the environmental adaptation toughness and the engineering availability of the pipe network system are obviously enhanced.

The invention belongs to the technical field of organic fertilizers, discloses a resource utilization method of compost leachate, and solves the technical problem that nitrogen in the compost leachate is not fully utilized in the prior art. The resource utilization method of the compost leachate comprises the following steps: step A, adding organic nitrogen mineralization promoting compound bacteria into the compost leachate, and carrying out aerobic reaction to decompose organic nitrogen to obtain aerobic reaction liquid; step B, adding anaerobic myxobacteria into the aerobic reaction liquid for anaerobic reaction to convert nitrate nitrogen into ammonium nitrogen, so as to obtain anaerobic reaction liquid; step C, adding citric acid-ferrous modified charcoal into the anaerobic reaction liquid to adsorb ammonium nitrogen, and filtering to obtain ammonium nitrogen loaded charcoal; step D, desorbing the ammonium nitrogen loaded charcoal by using a NaCl solution, and collecting a release solution and desorbed charcoal; and step E, carrying out concentration and crystallization treatment on the release liquid to obtain ammonium chloride crystals.

The invention discloses application of lactobacillus reuteri in preparation of a medicine for preventing and treating colitis. Lactobacillus reuteri LR1 can act on at least two of the following items: regulating protein expression of NLRP3, Caspase-1 and IL-18 in colon tissues; the relative abundance of bacteroides, actinomycetes and myxomycetes in intestinal flora is changed; the content of intermediary amines, hypoxanthine and inosine in colon tissues is influenced; the colitis comprises ulcerative colitis, Crohn's disease and DSS-induced experimental colitis. The invention relates to the technical field of biological medicines. According to the application of the lactobacillus reuteri in preparation of the medicine for preventing and treating the colitis, the processes of enteric coating, microencapsulation and the like are adopted, an acrylic resin IV coating material and a sodium alginate-CaCl2 curing system are matched, lactobacillus reuteri LR1 can be prevented from being damaged by gastric acid, viable bacteria are ensured to directly reach colon to play a role, meanwhile, the survival rate of the viable bacteria is larger than or equal to 90% through the freeze-drying process, and the survival rate of the viable bacteria is larger than or equal to 90%. After an acceleration test is performed for 6 months, relatively high activity is still maintained, and stable pesticide effect is guaranteed.

The invention relates to a soil fertility inversion method and system based on an improved myxomycete algorithm, and the method comprises the steps: collecting a hyperspectral remote sensing image of a target region, and obtaining a remote sensing image only containing soil pixels through an SVM; performing spectral feature transformation to obtain a high-dimensional spectral feature set, performing spectral feature optimization based on an improved myxobacteria optimization algorithm to obtain an optimal feature subset, and inputting a pre-trained statistical regression model between spectral features and soil parameters to obtain a soil fertility spatial distribution diagram of the target area; according to the improved myxomycete optimization algorithm, a priori Chebyshev initialization strategy fusing PLS and Lasso coefficients is adopted, a multi-level position updating strategy and a reverse learning and sparrow search algorithm are adopted to obtain a high-quality feature subset, a PLS weight coefficient is utilized to perform preliminary screening to obtain a strong correlation feature subset, a composite fitness calculation function is utilized to perform quality evaluation, and a high-quality feature subset is obtained. And obtaining an optimal feature subset. Compared with the prior art, the method improves the applicability and accuracy of soil fertility inversion.

The application discloses a myxobacterium MyxoALP1 protein and application thereof. The application obtains a myxobacterium MyxoALP1 protein with an ApeC-like domain in bacteria, the ApeC-like domain of the protein has similar characteristics with ApeC in invertebrates, and four conserved cysteines and three DXED motifs are reserved. Research shows that the myxobacterium MyxoALP1 recombinant protein has the ability of binding and coagulating bacteria and fungi, can bind various bacteria well, has broad-spectrum binding and coagulation activity, can be used for coagulation and removal of bacteria and fungi through the binding and coagulation of bacteria, can be used for preparing a drug for treating bacterial infectious diseases, and provides a new selection for the development of a product and a medicament for binding and coagulating bacteria.

The invention discloses a bio-organic fertilizer containing a compound microbial agent, and relates to the field of bio-organic fertilizers, and a preparation method of the bio-organic fertilizer comprises the processes of raw material pretreatment, microbial agent compounding, staged fermentation and post-treatment. According to the method, chicken manure, peanut shells and corn straw are used as raw materials to form an organic matrix, and the organic matrix is mixed with ground phosphate rock, lignite extract and nano SiO2 after being subjected to microwave pretreatment and NaOH solution soaking. The complex microbial inoculant is prepared by compounding bacillus subtilis, anabaena, pseudomonas fluorescens and myxobacteria and is fixed by a modified montmorillonite carrier. The fermentation process is divided into two stages: for primary fermentation, oxygen is controlled by adopting a PDMS (Polydimethylsiloxane) microporous membrane, the temperature is firstly increased to 50 DEG C and is maintained for 48 hours, then the temperature is increased to 65 DEG C and is maintained for 24 hours, and the turning interval is 24 hours; and carrying out secondary fermentation, cooling to 40 DEG C, inoculating myxobacteria suspension, and thoroughly decomposing for 7 days. The post-treatment comprises granulation, coating and drying, the viable count of the final product is greater than or equal to 2 * 10 < 8 > CFU / g, and the organic matter is greater than or equal to 45%.

This invention discloses a highly efficient and scarless method for editing the genome of myxobacteria and its applications. This invention combines the ISDra2 TnpB cleavage system of *Gastrococcus radiodurans* with the MxRedET homologous recombination system of myxobacteria to form a one-step scarless editing platform (MxDIRECT). ISDra2 is responsible for precisely locating and cleaving the target DNA, and MxRedET then efficiently integrates or replaces fragments at the cleavage site, enabling gene knockout, insertion, and replacement operations. This method increases the editing efficiency of myxobacteria from 1% to 100%, shortens the cycle from 3-6 months to 5-7 days, and allows for one-step two-site scarless editing through a two-site design. A single cleavage can knock out up to approximately 80kb of DNA, while two cleavages can knock out up to 200kb; and single nucleotide substitutions can be performed at any location, achieving precise base editing. This technology provides an efficient and precise tool for modifying the functional genes and metabolic pathways of myxobacterial chassis cells, significantly advancing synthetic biology research.