Sulfate reducing bacteria resisting microbial preparation and preparation method thereof

A microbial inoculum and sulfate-resistant technology, applied in the field of biological products, can solve problems such as difficulty in obtaining control effects, high control costs, and environmental pollution, so as to protect the ecological environment and reduce water treatment costs and processes. simple effect

Inactive Publication Date: 2008-08-06
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the use of chemical agents not only increases the amount of dosing, but also increases the cost of prevention and control, and causes environmental pollution, making it difficult to achieve the expected control effect.

Method used

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  • Sulfate reducing bacteria resisting microbial preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Isolation, enrichment and antibacterial effect of myxobacteria:

[0040] A strain of myxobacteria isolated from the soil is cultivated in a mixed medium, and the mass content of each component of the mixed medium is: K 2 HPO 4 (0.1%), CaCl 2 (0.1%), yeast powder (0.2%), peptone (0.2%), glucose (1%), bean sprouts juice (0.2%) to adjust the pH to be 7.0~7.5. Then, after culturing at 37° C. for 5 to 7 days, centrifuge to obtain the supernatant and bacterial cells. The test results showed that the minimum bacteriostatic dose was 3 mL, and the minimum bacteriostatic value was 0.5 g. The test results of the strain obtained after a series of medium optimization showed that the minimum inhibitory dose was 1 mL, and the minimum inhibitory value was 0.3 g.

Embodiment 2

[0041] Example 2. Individual detection results of bacteria species such as Streptomyces orientalis: See Table 1 for specific results.

Embodiment 3

[0042] Embodiment 3. mixed culture and domestication method of mixed bacterial liquid:

[0043] Two or more of the beneficial bacteria involved in the present invention are added to the mixed medium for fermentation and culture through mutagenesis or culture medium optimization, that is, in K 2 HPO 4 (0.05~0.1%), MgSO 4 ·7H 2 O (0.01~0.05%), CaCl2 (0.05~0.1%), yeast powder (0.1~0.2%), peptone (0.1~0.3%), glucose (0.1~0.3%) adjust pH to be 6.8~7.2 and cultivate 3~ 7 days. Inoculate into the domestication medium for domestication. The domestication method is: dilute the oilfield reinjection water to be treated into the mixed medium step by step at a ratio of 5-90%, and add at a dilution of 5% for each level. The type of bacteria is selected and the culture time is 2 to 7 days each time to obtain a mixed engineering bacteria group that can be directly added to the reinjection water.

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Abstract

The invention relates to a microbial agent of sulfate-reducing bacteria, which utilizes bacteria which can secrete antibiotic namely one or several of myxobacteria, streptomycete and bacillus to form single or mixed bacterial liquid product through culturing, or form single or mixed fermentation liquor after fermenting and extracting concentrated solution products. The process for preparing the microbial agent comprises the following steps: inoculating one or several of myxobacteria, streptomycete and bacillus whose bacterial strain is activated through a slant face on an optimum culture medium to culture, and then obtaining through project domestication and scale-up culture, or obtaining through fermenting the bacterial strain under pure culture condition and a mixed culture condition. The microbial agent can completely inhibit harmful bacterium from producing in membrane deposit, which can reduce the employment of chemical agent, and is beneficial for the protection of ecologic environment, and the microbial agent is particularly suitable for the prevention and cure of sulfate ash reducing bacteria corrosion in an oil field water re-injection system, various recycle water systems and a central air-conditioning cooling water system, preparation technology is simple, and the invention is easy to implement.

Description

technical field [0001] The invention relates to a biological product capable of inhibiting or killing sulfate-reducing bacteria in metal pipelines, in particular to a microbial agent with high efficiency against sulfate-reducing bacteria and a preparation method thereof. Background technique [0002] In oilfield reinjection water systems, various circulating water systems, and central air-conditioning cooling water systems, there are a certain number of harmful bacteria and various nutrient sources suitable for their growth. Especially in the oilfield water injection system, there are three main types of bacteria that are most harmful, namely, sulfate-reducing bacteria (Sulfate-reducing Bacteria, SRB), iron bacteria (Iron Bacteria, IB) and saprophytic bacteria (TGB). These three types of bacteria use the scaling products such as calcium scale and iron scale existing on the pipe wall, and use each other's metabolites and corrosion products to form biofilm scale on the metal p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/465C12R1/07
Inventor 陈悟曾庆福陈洪高陈海英李海燕夏东升王军
Owner WUHAN TEXTILE UNIV
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