7 results about "Bacteriology" patented technology

The invention discloses a PCR-HRM kit for identifying Salmonella pullorum and Salmonella gallinarum and its application. The kit comprises PCR-HRM primers, and the primer nucleotide sequences are as follows: upstream primer fimH665-77F: 5'-GGTCGTGGAGTTTGATTTCG-3'; downstream primer fimH665-77R: 5'-CGCCTTGCGGACGATTAC-3'. The invention establishes a PCR-HRM detection kit for rapid identification of Salmonella pullorum and Salmonella gallinarum based on the specific SNP site of the fimH gene. The kit of the invention has the advantages of simple operation and high-throughput detection, and can significantly shorten the time required for identification of Salmonella pullorum and Salmonella gallinarum. At the same time, it has strong specificity and high sensitivity. Compared with traditional bacteriological identification methods, it has great advantages in detection time and detection cost, and is expected to have broad application prospects in rapid differential diagnosis of Salmonella pullorum and Salmonella gallinarum.

The application belongs to the field of animal bacteriology and molecular biology technology, and relates to preparation of a hybridoma cell and application thereof. A monoclonal antibody is prepared through intracellular Lawsonia intracellularis outer membrane protein OmpA expressed by prokaryotes, and IFA, WB and blocking ELISA and other immunological detection methods for detecting or diagnosing Lawsonia intracellularis are established through the expressed intracellular Lawsonia intracellularis outer membrane protein OmpA and the prepared monoclonal antibody. The blocking ELISA for detecting serum antibodies of Lawsonia intracellularis can detect serum samples of suspected Lawsonia intracellularis infection from different species, and does not exist cross reaction with positive serum of common pig pathogens, has high sensitivity and specificity, is good in repeatability, and is suitable for high-throughput detection of serum samples.

This invention is related to a training set used in the culturing processes of microorganisms and designed to be used for educational purposes, characterized in that it comprises a transparent plastic medium (1), a template (3) which is attached or fixed to the bottom of the medium (1) and contains microbiological planting images thereon, a syringe (2) which enables a microbiological planting process to be carried out on the medium (1) based on the images on the template (3), dye tubes (4) containing different colors of dye, and a bag set (5) containing a plastic medium (1), a syringe (2), a template (3) and dye tubes (4).

A bacteria test method according to the invention tests for the presence or absence of bacteria in a sample, and includes amplifying a conserved sequence in bacterial 23S rRNA by PCR using a primer set consisting of a primer (1) and a primer (2). Primer (1): A primer having a sequence of SEQ ID NO: 2, and / or a primer having a sequence in which one to three bases have been modified in the sequence of SEQ ID NO: 2. Primer (2): At least one selected from the group consisting of primers having a sequence of SEQ ID NO: 3, primers having the sequence of SEQ ID NO: 3 in which one to three bases have been modified, primers having a sequence of SEQ ID NO: 36, primers having the sequence of SEQ ID NO: 36 in which one to three bases have been modified, primers having a sequence of SEQ ID NO: 39, primers having the sequence of SEQ ID NO: 39 in which one to three bases have been modified, primers having a sequence of SEQ ID NO: 41, and primers having the sequence of SEQ ID NO: 41 in which one to three bases have been modified.

This invention is related to a training set used in the culturing processes of microorganisms and designed to be used for educational purposes, characterized in that it comprises a transparent plastic medium (1), a template (3) which is attached or fixed to the bottom of the medium (1) and contains microbiological planting images thereon, a syringe (2) which enables a microbiological planting process to be carried out on the medium (1) based on the images on the template (3), dye tubes (4) containing different colors of dye, and a bag set (5) containing a plastic medium (1), a syringe (2), a template (3) and dye tubes (4).

The present invention discloses a chitinase KPGH3 for inhibiting biofilm synthesis, destroying biofilm structure, or in antibacterial application. KPGH3 can be any of the following proteins: A1) a protein whose amino acid sequence is sequence 3 in a sequence list; A2) a protein whose amino acid sequence is sequence 5 in a sequence list; A3) a protein having more than 90% identity with the protein shown in A1) and having chitinase activity obtained by replacing and / or deleting and / or adding one or more amino acid residues of the protein of A1) or A2); A4) a fusion protein obtained by connecting a protein tag at the N-terminus or / and C-terminus of A1) or A2). KPGH3 has the potential to inhibit and disperse biofilms of various pathogenic microorganisms and the potential chitin biotransformation ability, and has the feasibility of using it to develop new anti-biofilm therapeutic agents.