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Kit and detecting method for quickly detecting mutiplex PCRs of five common pathogenic bacteria in disposable hygiene products

A technology for hygiene products and pathogenic bacteria, applied in the biological field, can solve problems such as unsatisfactory rapid detection and large detection volume

Inactive Publication Date: 2015-05-13
TECHN CENT FOR SAFETY OF INDAL PRODS TIANJIN ENTRY EXIT INSPECTION QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many single PCR rapid detection methods for multiple pathogenic bacteria have been established, such as Salmonella, Listeria monocytogenes, etc. detection, and the amount of detection is large, obviously it cannot meet the needs of daily rapid detection

Method used

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  • Kit and detecting method for quickly detecting mutiplex PCRs of five common pathogenic bacteria in disposable hygiene products
  • Kit and detecting method for quickly detecting mutiplex PCRs of five common pathogenic bacteria in disposable hygiene products
  • Kit and detecting method for quickly detecting mutiplex PCRs of five common pathogenic bacteria in disposable hygiene products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: After autoclaving the samples Point (application number: 1200170213004740), Merries (application number: 1200170213006229) and Petpad (application number: 1200170213004629), they were artificially contaminated with random pathogenic bacteria. The multiplex PCR detection results of the artificially contaminated samples Point, Merries and Petpad are shown in Figure 14 , Figure 15 and Figure 16 . The experimental results showed that the LB culture medium obtained a better effect of bacteria enrichment. PCR can effectively randomly amplify the corresponding target gene fragments, so that any two contaminated bacteria can be identified. Among them, the detection limit of Salmonella in the simulated positive samples Point, Merries and Petpad can reach 6×104 The detection limit of cfu / ml and Staphylococcus aureus can reach 2×10 5 The detection limit of cfu / ml and Pseudomonas aeruginosa can reach 1.5×10 5 The detection limit of cfu / ml and Escherichia coli can...

Embodiment 2

[0046] Example 2, The PCR test results of the three diapers and roll papers in the experiment showed that Salmonella and Escherichia coli were detected in two diapers, among which Salmonella was detected in Point (application number: 1200170213004740), and Salmonella was detected in Petpad ( Inspection number: 1200170213004629) Escherichia coli was detected. Staphylococcus aureus was detected in two facial tissues, roll A and roll C respectively, the results are shown in Figure 17 .

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Abstract

The invention relates to a kit and detecting method for quickly detecting mutiplex PCRs of five common pathogenic bacteria in disposable hygiene products. The kit comprises nuclease-free water, 10*PCR buffer solutions, dNTP, upstream-downstream combined primers, DNA polymerases, negative quality control products and positive quality control products. The kit provided bt the technical scheme of the invention has a favorable detection specificity, and can realize quick detection of salmonella, pseudomonas aeruginosa, staphylococcus aureus, colibacillus and candida albicans, and as compared with conventional bacteriology, the diagnosis is quick, simple, convenient and economy.

Description

technical field [0001] The invention relates to a kit and a detection method for multiplex PCR rapid detection of five common pathogenic bacteria in disposable sanitary products, belonging to the field of biotechnology. Background technique [0002] The safety of sanitary products is a major concern in China, and susceptibility to pathogenic bacteria is one of the main reasons affecting the safety of sanitary products. Salmonella spp, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli (E.coli) and Candida albicans are ubiquitous in sanitary products and are the main causes of contamination of sanitary products important factor. According to the current national hygiene product safety supervision system, Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Candida albicans are the detection items in many hygiene product standards, and they are not required to be detected. At present, the traditional bacteriological culture method is m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/445C12R1/42C12R1/01C12R1/725C12R1/19
CPCC12Q1/689C12Q1/686C12Q2600/16
Inventor 赵琢刘志鹏王华柳明
Owner TECHN CENT FOR SAFETY OF INDAL PRODS TIANJIN ENTRY EXIT INSPECTION QUARANTINE BUREAU
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