177 results about "Candida albican" patented technology

The invention provides lactobacillus crispatus, which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on February 7, 2023, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 26503. The bacillus subtilis can play a relatively strong role in inhibiting pathogenic bacteria such as gardnerella vaginalis, escherichia coli and staphylococcus aureus at the same time, and also has a certain role in inhibiting candida albicans, two-way proteurella and Atortoria vaginalis. Therefore, the lactobacillus crispatus has the potential of maintaining the environment in the vagina to be faintly acid and improving the mucosal immunity and infection resistance of a host, and can be used for preventing and treating vaginal infection and inflammation, and maintaining, improving or recovering the steady state of the environment in the vagina.

The invention discloses a bacillus strain H7 and an application thereof. The invention provides bacillus velezensis H7 or a progeny of the bacillus velezensis H7, and the preservation number of the bacillus velezensis H7 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 2025630. The bacillus velezensis H7 disclosed by the invention has a very good antibacterial effect on various common clinical pathogenic bacteria (including klebsiella pneumoniae, escherichia coli, candida albicans, enterobacter cloacae complex, acinetobacter baumannii and the like), and particularly shows a very high inhibition rate on some drug-resistant clinical pathogenic bacteria; therefore, the compound has important application value in development of drug and non-drug antibacterial products (such as medical antibacterial materials and the like), a new choice can be provided for drug and non-drug prevention and control of clinical pathogenic bacterium infection, and particularly, the compound has a supplementary effect in the aspects of reducing drug-resistant bacterium propagation risk and reducing drug dependence.

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The invention relates to the technical field of medicines, in particular to application of tedizolid combined with fluconazole in preparation of antifungal drugs. The tedizolid and the fluconazole are combined for use, so that the antibacterial activity of the fluconazole on the candida albicans can be enhanced, a synergistic antifungal effect can be generated, and the drug resistance of the candida albicans on the fluconazole can be reversed.

The invention relates to the technical field of PA resin, and particularly discloses glass fiber modified antibacterial PA resin suitable for children products and a preparation method of the antibacterial PA resin. The invention discloses glass fiber modified antibacterial PA resin suitable for children products. The glass fiber modified antibacterial PA resin comprises PA resin, glass fibers, talcum powder, nano calcium carbonate, a composite antibacterial agent, a compatilizer, an antioxidant and calcium stearate, the composite antibacterial agent comprises chitosan, an antibacterial peptide microcapsule and a porous silver-zinc-loaded antibacterial agent; the antibacterial peptide microcapsules are sodium alginate embedded antibacterial peptides. The antibacterial PA resin obtained in the invention has the highest bacteriostasis rates of 99.97%, 99.74% and 99.95% for staphylococcus aureus, escherichia coli and candida albicans respectively, the antibacterial property of the PA resin is improved, the compressive strength and notch impact strength are 170.6 MPa and 17.3 kJ / m < 2 > respectively, and high mechanical strength is maintained on the basis of high antibacterial property.

The invention relates to lactobacillus gasseri FMTA1121E with an effect of regulating a vaginal micro-ecological environment and application of the lactobacillus gasseri FMTA1121E. The lactobacillus gasseri FMTA1121E is classified and named as lactobacillus gasseri, and the preservation number of the lactobacillus gasseri FMTA1121E is CGMCC No.34962. The lactobacillus gasseri FMTA1121E can be metabolized to generate beneficial components such as lactic acid, gamma-aminobutyric acid and hydrogen peroxide so as to regulate the genital tract environment and improve the health degree; and the bacillus subtilis has excellent bacteriostasis and sterilization effects on various common vaginal pathogenic bacteria such as escherichia coli, staphylococcus aureus, gardnerella vaginalis, candida albicans, neisseria gonorrhoeae and the like. Therefore, the lactobacillus gasseri FMTA1121E strain can be applied to preparation of preparations for preventing and treating related genital tract diseases such as vaginitis.

The invention relates to a talaromyces purpureogenus strain D01 and application of the talaromyces purpureogenus strain D01, and belongs to the technical field of microorganisms. The talaromyces purpurogenus D01 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the talaromyces purpurogenus D01 is GDMCC NO.66458. The strain is obtained by separating and purifying soil samples near exits of Guangzhou Taitai and subway stations, and tests show that bacteriostatic active substances generated by fermentation of the strain have good bacteriostatic effects on burkholderia cepacia, staphylococcus aureus, pseudomonas putida, pseudomonas aeruginosa, escherichia coli and candida albicans. And the antibacterial active substance has high temperature resistance and excellent preservative effect, can be used as an antibacterial preservative raw material to be applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

The invention belongs to the technical field of biology, and particularly relates to a primer probe composition and a kit for detecting lower respiratory tract infection pathogens. The primer probe composition is used for detecting mycobacterium tuberculosis, mycobacterium avium, mycobacterium abscessus, mycobacterium kansasii, pseudomonas aeruginosa, klebsiella pneumoniae, acinetobacter baumannii and haemophilus influenzae, and the primer probe composition is used for detecting mycobacterium tuberculosis, mycobacterium avium, mycobacterium abscessus, mycobacterium kansasii, pseudomonas aeruginosa, klebsiella pneumoniae, acinetobacter baumannii and haemophilus influenzae. The composition is one or more of a composition for detecting staphylococcus aureus, a composition for detecting streptococcus pneumoniae, a composition for detecting escherichia coli, a composition for detecting moraxella catarrhalis, a composition for detecting serratia marcescens, a composition for detecting mycoplasma pneumoniae, a composition for detecting candida albicans or a composition for detecting adenovirus; the RAA-RDB technology has the core advantages of rapidness, high sensitivity, low cost and easy operation, and is especially suitable for multiple pathogen screening and basic medical application.

The invention relates to bark-like nano-zinc oxide and a preparation method thereof, and belongs to the technical field of liquid-phase synthesis of nano-zinc oxide. According to the invention, the nano zinc oxide material with bark-like microstructure is prepared by regulating and controlling the liquid phase environment and the thermodynamic and kinetic parameters of the reaction. The material has obvious layering sense and pore characteristics, the material is endowed with significantly enhanced physicochemical characteristics, for example, the BET specific surface area is significantly increased, and meanwhile, the high porosity and the nanocrystal boundary effect have a synergistic effect, so that active oxygen species and zinc ion antibacterial active species can be efficiently generated, and the antibacterial performance of the material is significantly improved. An antibacterial experiment shows that the antibacterial rate of the material to escherichia coli and staphylococcus aureus exceeds 99.9%, and the antibacterial rate of the material to candida albicans exceeds 99.0%. The material has application potential in the fields of medical dressings, antibacterial textiles, water purification and the like.

The invention provides a plant fiber containing a ginger extract and a preparation process of the plant fiber. After the plant fiber is washed for 50 times, the bacteriostasis rates of the plant fiber to staphylococcus aureus, escherichia coli, klebsiella pneumoniae and candida albicans are all greater than or equal to 95%, the killing rates of the plant fiber to influenza viruses H3N2 and H1N1 are all greater than or equal to 99%, the far infrared emissivity is 0.89-0.95, the highest temperature rise of moisture absorption and heating is 4-12 DEG C, and the average temperature rise is 3-8 DEG C. A vapor deposition method is adopted, a biomass component A and a biomass component B are combined to form a biomass component C, on one hand, the biomass component B serves as a tube bundle-shaped base material, rhizome plants are calcined to form a porous carbon structure framework, the porous carbon structure framework serves as a solid for vapor deposition, and a solution containing the biomass component A is small in gas molecular weight; after being vaporized along with the solution, the solution is caught by a porous structure of the biomass component B and uniformly filled into pores of the biomass component B, so that the durability of plant functions is ensured, and meanwhile, a protection effect at high temperature is achieved.

The invention belongs to the technical field of microbiological detection, and particularly relates to a real-time fluorescent quantitative PCR (polymerase chain reaction) primer, a probe and a kit for detecting a special drug-resistant pedigree of candida albicans. The nucleotide sequence of the specific primer F is as shown in SEQ ID NO. 1; the nucleotide sequence of the specific primer R is as shown in SEQ ID NO. 2; the nucleotide sequence of a probe matched with the primer for use is as shown in SEQ ID No.3. The kit prepared by the invention ensures high specificity and high sensitivity in a PCR amplification process, effectively distinguishes drug-resistant mutant strains from sensitive strains, keeps efficient detection capability in a low-copy-number DNA template, reduces non-specific amplification and background signal interference, and improves the reliability and accuracy of a detection result.

The present application relates to the field of pharmaceutical chemistry, and specifically discloses a emodin mannich base, a preparation method and application thereof. The emodin mannich base has a structure as shown in formula I, the structure is simple, has strong in-vitro antimicrobial activity, especially high inhibitory activity to staphylococcus aureus, bacillus subtilis, micrococcus luteus, escherichia coli, proteus vulgaris, pseudomonas aeruginosa, shigella dysenteriae, salmonella typhimurium and other bacteria, and candida utilis, candida albicans, saccharomyces cerevisiae, monascus and other fungi, and can be used for preparing antibacterial and / or antifungal drugs.

The invention belongs to the technical field of separation and purification, and particularly relates to a separation and purification method for active components of wasp venom. According to the invention, only an ultrafiltration membrane method is adopted, reverse operation is carried out according to a sequence from large to small, ultrafiltration membranes with molecular weight cutoff of 30kDa, 10kDa and 3kDa are respectively used for sequentially intercepting a vespa velutina venom solution, and freeze drying is carried out, so that three active components with antifungal (candida albicans) and antibacterial (escherichia coli and staphylococcus aureus) effects are obtained. The method is simple to operate and relatively high in efficiency, purification time is greatly saved, the whole process only relates to physical separation, inactivation of active components is greatly avoided, and the method is very beneficial to process amplification.

The invention relates to a lactobacillus plantarum, the name of the lactobacillus plantarum is lactobacillus plantarum 021, the classification name of the lactobacillus plantarum is lactobacillus plantarum, the preservation number is CGMCC No.22169, the preservation date is April 12, 2021, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.22169, the preservation number is CGMCC No.22169, the preservation number is CGMCC No.22169, the preservation number is CGMCC No.22169, the preservation number is CGMCC No.22169, the preservation number is CGMCC No.22169, and the preservation number is CGMCC No.22169. And Institute of Biology, Chinese Academy of Sciences, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. After the lactobacillus plantarum 021 is mixed with the water-absorbent resin, the growth activity of the lactobacillus plantarum 021 is not inhibited, and meanwhile, the lactobacillus plantarum 021 keeps relatively strong inhibition capability on common pathogenic bacteria, such as candida albicans, staphylococcus aureus and escherichia coli. Therefore, the lactobacillus plantarum 021 disclosed by the invention can be applied to preparation of sanitary products such as sanitary towels and tampons after being mixed with water-absorbent resin.

The invention relates to the technical field of pharmaceutical preparations, in particular to application of phencycloqualinium bromide as an antibacterial active component and a preparation of the phencycloqualinium bromide. It is found that benzene ring quinaldinium bromide can be used as an antibacterial active component and has an inhibiting effect on various common bacteria and fungi (including but not limited to staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, aspergillus niger, candida albicans and the like); triethanolamine or other bacteriostatic activity enhancers are mixed with the phencycloqualinium bromide, so that the bacteriostatic activity of the phencycloqualinium bromide can be remarkably improved; when a benzene ring quinaldinium bromide related preparation is prepared, other bacteriostatic agent components are not added, a good bacteriostatic effect can still be achieved, and the safety is higher.

Described herein are Staphylococcal toxins inspired peptides (STIPs) with antimicrobial activity, compositions and kits comprising the peptides, and use of the peptides to treat microbial infections. Also described are methods of inhibiting or preventing the growth of a microorganism, and methods of treating microbial infections such as bacterial infections including MRSA, and fungal infections including C. albicans.

The invention relates to an antibacterial gel and a preparation method thereof. The gel mainly comprises silver ions, avocado oil, a radix puerariae extract, wheat germ oil, emulsifying wax, a folium artemisiae argyi extract, glycerol, a styrene / butadiene copolymer, purified water and hydroxyethyl cellulose. Extracts containing specific small-molecule polypeptides are extracted from Artemisia rubescens and Radix Puerariae through a specific extraction method, and the polypeptides are Artemisia rubescens polypeptides (SEQ ID No: 1) and Radix Puerariae polypeptides (SEQ ID No: 2) respectively. The extracts have a synergistic effect with silver ions, so that the antibacterial effect of the gel is remarkably improved, and particularly, the gel has a remarkable inhibiting effect on staphylococcus aureus, escherichia coli and candida albicans. The bacteriostatic gel is simple in preparation method, convenient to use and suitable for bacteriostasis of female vaginal mucosa and maintenance of vaginal micro-ecological internal environment.

The invention belongs to the technical field of microorganisms, and discloses bacillus subtilis with a high nattokinase yield and a biological preservation function and application of the bacillus subtilis. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34222. The serine protease gene of the strain carries specific mutations (SEQ ID NO: 1) which are mutated from G to C at the 217th site and the 230th site, so that the yield and the activity of nattokinase are remarkably improved. In the fermentation process, through an induction stage (38 DEG C heat preservation for 8 hours), a main fermentation stage (50 DEG C heat preservation for 7 hours) and a cooling after-ripening stage (4 DEG C heat preservation for 24 hours), the enzyme activity of the prepared natto product is larger than or equal to 57028 IU / mL, and the natto product is light in flavor, excellent in wiredrawing effect and appropriate in taste. In addition, the strain can inhibit the growth of Candida albicans (the inhibition zone is greater than or equal to 25mm), and the shelf life of natto is effectively prolonged. The problems that an existing strain is poor in stability, products are prone to pollution, and the flavor is poor are solved.

The invention discloses a plant extract-containing composite antibacterial external spray for pets and a preparation method of the plant extract-containing composite antibacterial external spray, and belongs to the technical field of pet nursing materials. The external spray comprises the following components in percentage by mass: 0.5-1.0% of a cortex dictamni extract, 0.75-1.5% of a tomato fruit extract, 0.75-1.5% of a folium artemisiae argyi extract and 5-8% of anthemis nobilis hydrolat, and can also be matched with auxiliary components such as an emulsifier and a preservative. The cortex dictamni extract is prepared by combining a supercritical CO2 extraction method with a specific compound entrainer, and active ingredients are fully reserved; the spray is prepared through step-by-step preparation of a water phase and an oil phase and then high-shear emulsification, and the stability is good. The product shows a high bacteriostatic effect on staphylococcus aureus, salmonella and candida albicans, can significantly reduce the content of an inflammatory mediator NO and a proinflammatory factor IL-1beta, has bacteriostatic, bactericidal and anti-inflammatory effects, is suitable for preparation of pet skin care related products, and has a wide application prospect.

The present application provides a kind of endo beta-1, 3-glucanase and its application, the amino acid sequence of the endo beta-1, 3-glucanase is SEQ ID NO:1.The endo beta-1, 3-glucanase of the present application can efficiently hydrolyze laminarin and curdlan, its enzyme activity can still be kept above 80% in the range of 40-60 DEG C and pH 4.5-6.0, and it shows good stability under low temperature conditions, which is conducive to reduce the energy consumption in the process of practical application.At the same time, the enzyme has significant high salt tolerance, and has certain resistance to 5%-15% ethanol.The endo beta-1, 3-glucanase of the present application has obvious inhibitory effect on the formation of Candida albicans biofilm, and the laminarin oligosaccharide generated by hydrolyzing laminarin also shows good antioxidant capacity, indicating that the enzyme has broad application prospect and significant practical value in the fields of antibacterial, biological medicine and the like.

The invention relates to the technical field of biology, and discloses an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID NO: 1, the antibacterial peptide shows antibacterial activity to escherichia coli, staphylococcus aureus and candida albicans, especially shows high antibacterial activity to the candida albicans, the minimum inhibitory concentration is 64 [mu] M, and the antibacterial peptide has good antibacterial activity. The compound can be used for preparing bacteriostatic products such as bacteriostatic agents, bacteriostatic drugs and the like for resisting escherichia coli, staphylococcus aureus and candida albicans.

The invention belongs to the technical field of papermaking, and relates to aramid paper and a preparation method thereof. According to the preparation method, aramid fibrid and ANF fiber are jointly used as raw materials, and the structural formula of ANF is as follows: # imgabs0 #, in the formula, n: m = (0.1-0.4): (0.6-0.9), m + n = 1, and-X is-F,-Cl,-Br or-I. The basis weight of the finally prepared aramid paper is 60 + / -1 g / m < 2 >, the thickness is 0.062-0.065 mm, the tensile strength is 4.58-5.5 kN / m, the tensile index is 48.1-51 N.m / g, the elongation at break is 7.88-8.9%, the electrical strength is 25-45 kV / mm, the effective diameter of an inhibition zone for staphylococcus aureus is 1.86-1.99 mm, the effective diameter of an inhibition zone for escherichia coli is 2.01-2.45 mm, and the effective diameter of an inhibition zone for candida albicans is 3.77-4.21 mm. The aramid paper has high insulativity and excellent mechanical property and antibacterial property, and the preparation method is simple and suitable for large-scale application.

The invention relates to the technical field of antibacterial composition preparation, in particular to a gynecological antibacterial composition and a preparation method thereof, and the gynecological antibacterial composition comprises the following raw materials: modified radix sophorae flavescentis, angelica sinensis, modified cortex phellodendri, radix paeoniae rubra, ligusticum wallichii, bombyx batryticatus, salvia miltiorrhiza, safflower carthamus, fructus cnidii, gallnut, calamine, pomegranate rind, catechu, rhizoma atractylodis, dragon's blood, fructus kochiae, borneol, alum, dried alum, purpurea sal ammoniac, magnesium stearate and starch. According to the invention, after the modified radix sophorae flavescentis is subjected to ethanol ultrasonic extraction, purification and micronization treatment, the dissolution rate and purity of effective components such as sophocarpidine are improved, the cell membrane structure of gram-negative bacteria such as escherichia coli can be quickly destroyed, and efficient bacteriostasis is realized; the modified cortex phellodendri is subjected to alkali dissolution extraction, acid precipitation purification and refined drying, berberine hydrochloride is efficiently enriched, the purity is improved, and the berberine hydrochloride can kill fungi such as candida albicans more accurately and strongly; and through high-temperature calcination and acetic acid activation, the calamine and the catechu are tightly combined at a microscopic level, so that the inherent convergence, granulation promoting and antibacterial effects of the calamine and the catechu are enhanced.

The invention belongs to the technical field of medicines, and discloses a gynecological bacteriostatic lotion and a preparation method and application thereof.The lotion is prepared from extracts of traditional Chinese medicinal materials such as radix sophorae flavescentis, cortex phellodendri, coptis chinensis, scutellaria baicalensis, rhizoma smilacis glabrae, cortex dictamni, fructus cnidii, areca nuts, cortex meliae, golden larch bark, lithospermum erythrorhizon, lavender, rheum officinale and honeysuckle as main raw materials, and parachloroxylenol, salicylic acid and borneol as auxiliary materials. A compound preparation with multiple effects is formed through scientific proportioning and a preparation process, the product can effectively inhibit common pathogenic bacteria such as Candida albicans, Staphylococcus aureus and Escherichia coli, shows a broad-spectrum and efficient antibacterial effect, and is small in irritation, high in safety to vaginal mucosa and genital tract environment and free of toxic and side effects. The product can maintain local micro-ecological balance and reduce inflammation recurrence while cleaning and nursing, has the advantages of long-term conditioning of a traditional Chinese medicine compound and rapid sterilization of western medicine components, and is suitable for clinical popularization and application.

The invention belongs to the technical field of biological model construction, and particularly relates to a method for constructing a vasculitis model in zebra fish. The compound is obtained by inducing a Candida albicans water soluble substance (CAWS), and the specific method is micro-injection or soaking. According to the method, whether modeling succeeds or not can be judged in the living body state of the model animal, the method is more suitable for visually observing the inflammation occurrence position, a research method for vasculitis (including Kawasaki disease) is developed, follow-up scientific research data can be richer and more accurate, and a more solid theoretical basis is laid for clinical transformation of targeted therapy of vasculitis (including Kawasaki disease).

The invention belongs to the technical field of microorganisms, and particularly relates to a bacillus altitudinis strain and application thereof, the bacillus altitudinis strain is a bacillus altitudinis strain H50 and has been preserved in China General Microbiological Culture Collection Center (CGMCC) on December 16, 2024, and the preservation number is CGMCC No.33070. The bacillus altitudinis strain is a bacillus altitudinis strain H50 which has been preserved in China General Microbiological Culture Collection Center (CGMCC). The bacillus altitudinis strain H50 disclosed by the invention is a strain with broad-spectrum antibacterial activity, and the strain particularly has an obvious antagonistic effect on tritirachium album, so that effective treatment on tritirachium album and escherichia coli infection is realized.

The invention discloses an effective part for chemoradiotherapy-induced oral mucositis in traditional Chinese medicinal material white flower and a preparation process of the effective part. The effective part is identified as a flavonoid component through standard substance spectrogram comparison. Tests prove that the production process of the effective part is practical and feasible, and the total flavonoids in the finally obtained extracting solution are greater than or equal to 50%. Besides, pharmacodynamic studies show that the effective part has a remarkable bacteriostasis effect on staphylococcus aureus, escherichia coli and candida albicans, the bacteriostasis rate is larger than or equal to 80%, the effective part has a remarkable treatment effect on rat oral mucosa acute inflammation caused by chemoradiotherapy, and the application prospect of the effective part in the adaptation disease is shown.

The present application relates to the technical field of sterilization methods of raw panax notoginseng, and particularly relates to a sterilization method of raw panax notoginseng powder, which can quickly, efficiently and simply sterilize the raw panax notoginseng powder, and has been verified to have good killing effect on gram-negative bacteria such as pseudomonas aeruginosa, escherichia coli and staphylococcus aureus, and fungi such as candida albicans. More importantly, compared with the irradiation sterilization equipment, the equipment adopted by the present application is simple, easy to operate, and low in sterilization cost, and can effectively kill the microorganisms in the raw panax notoginseng powder while retaining the efficacy of the raw panax notoginseng powder. The method is simple, efficient, safe, inexpensive and economical, and has good development and application value.

The invention discloses a 2-(imidazo [1, 5-a] pyridine-1-yl)-1-methylpyridine-1-onium compound as well as a preparation method and an antibacterial and mildew-proof application thereof. The structural formula of the 2-(imidazo [1, 5-a] pyridine-1-yl)-1-methylpyridine-1-onium compound is shown as a formula (II), and the 2-(imidazo [1, 5-a] pyridine-1-yl)-1-methylpyridine-1-onium compound is easy to synthesize, shows good antibacterial and mildew-proof activity, has good antibacterial activity on staphylococcus aureus, escherichia coli, candida albicans and aspergillus fumigatus, serves as a novel antibacterial and mildew-proof agent, and has good application prospects. The method has great application value in the technical field of antibiosis. Formula (II)

The invention belongs to the technical field of in-vitro diagnostic reagents for rapid detection of human pathogenic microorganisms, and particularly relates to a kit for rapid combined detection of multiple pathogen nucleic acids related to a dyspnea pregnancy outcome and application of the kit. The pathogen is selected from one or more of escherichia coli, candida albicans, candida glabrata, group B streptococcus, streptococcus gonorrhoeae, chlamydia trachomatis or ureaplasma urealyticum, and the kit comprises a pathogen and lactobacillus rapid detection micro-fluidic chip. A primer group for detecting the pathogen and the lactic acid bacillus nucleic acid and an array consisting of an amplification control and a blank control are fixed on the micro-fluidic chip. According to the present invention, the microfluidic chip rapid detection technology and the nucleic acid amplification technology are adopted to achieve the purposes of rapid detection and multiple detection so as to rapidly and conveniently determine the poor prognosis risk of the pregnant woman at present, timely take the specific measures, and avoid the occurrence of the poor prognosis.