The invention discloses an Eleotridae fattening feed and a making method thereof. The feed comprises the following raw materials: vinegar soaked bean cake, cottonseed meal, pig hair powder, corn flour, dried porcine soluble, white fish meal, vinegar soaked rice bran, sugarcane stalk, Round Cardamon Seed, pea, water shield, apium graveolens, sea buckthorn, panax notoginseng, astragalus, purslane, dried ginger, Mesona chinensis, Silphium perfoliatum, Euphorbia hypericifolia, wine, grease, xanthan gum, a feed attractant, and a proper amount of water. The feed provided by the invention is meticulously designed according to the nutritional requirements and diet habits of Eleotridae, has the characteristics of reasonable collocation, nutrition and health, and adequate energy, and can promote Eleotridae growth and weight gain, prompt rapid growth, shorten the growth cycle, increase yield, and increase the economic benefits of culture.