1003 results about "Human placenta" patented technology

The present invention relates to collagenous membranes produced from amnion, herein referred to as a collagen biofabric. The collagen biofabric of the invention has the structural integrity of the native non-treated amniotic membrane, i.e., the native tertiary and quaternary structure. The present invention provides a method for preparing a collagen biofabric from a placental membrane, preferably a human placental membrane having a chorionic and amniotic membrane, by decellularizing the amniotic membrane. In a preferred embodiment, the amniotic membrane is completely decellularized. The collagen biofabric of the invention has numerous utilities in the medical and surgical field including for example, blood vessel repair, construction and replacement of a blood vessel, tendon and ligament replacement, wound-dressing, surgical grafts, ophthalmic uses, sutures, and others. The benefits of the biofabric are, in part, due to its physical properties such as biomechanical strength, flexibility, suturability, and low immunogenicity, particularly when derived from human placenta.

The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.

The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.

The invention relates to a Chinese medicinal preparation for treating bone fracture, which is prepared from 84 raw medicinal materials such as radix astragali, Chinese angelica, rehmanniae vaporata, rehmanniae praeparatum, suberect spatholobus stem, human placenta and the like. The medicament can be made into an oral preparation and external application preparation. A preparation method comprises the following steps: preparing an aqueous extract of the raw medicinal materials, and preparing an oral solution and a spraying agent from the aqueous extract; and preparing extracting pulvis from the aqueous extract, and preparing granules, pills, medicinal granules, tablets or capsules from the extracting pulvis. Raw medicinal powder also can be ground into raw powder pulvis which can be prepared into the granules, the pills, the medicinal granules, the tablets or the capsules, or the raw powder pulvis is prepared into external application ointment. In addition, the raw medicinal materials also can be infused to form medicinal liquor. The Chinese medicinal preparation has good curative effect on bone fracture diseases of different types, is mainly used for treating different types of fracture, muscles and bones breakage, traumatic injury, new and old incised wound and gunshot wound, bleeding and blood stasis which are caused by traumatism and other symptoms, and has the obvious curative effect on rheumatoid arthritis. The Chinese medicinal preparation is prepared from pure Chinese medicaments, and has convenient use, safety, reliability, no toxic or side effect and long-term use.

The present invention discloses the separation method of mesenchymal stem cell in human placenta. On the basis of past separation of tissue cell, the present inventor separates from placenta mesenchymal stem cell with high purity via perfusion process based on the special anatomical structure of placenta. Identification result shows that the mesenchymal stem cell separated from placenta has the biological characteristic and polydirectional differentiation capacity as the reported mesenchymal stem cell of marrow. Owing to the infantile cell component and wide source of placenta, like cord blood, the present invention will have wide clinical application foreground.

The invention discloses a human placenta, umbilical cord mesenchyme stem cell bank and its making method. The method includes the following steps: the first is taking human placenta, umbilical cord to test; smashing after cleaning by phosphate buffer; adding phosphate buffer to dilute; the second is adding collagenase to digest; the third is adding phosphate buffer; the fourth is adding trypsinization; the fifth is mixing the cell from the second and fourth, centrifuging, removing supernatant, cleaning by the phosphate buffer, centrifuging, and removing supernatant; the mesenchyme stem cell are gained; the sixth is freezing them in liquid nitrogen; saving by ABO / Rh subtype and HLA subtype; setting recallable cell information file. The invention is newborn storage placenta and umbilical cord mesenchyme stem cell. It can offer mesenchyme stem cell to treat the disease for personal, family and others.

The invention relates to a method for separating exosome from a human placenta-derived mesenchymal stem cell source. The method comprises the following steps: when the P2-P3 placenta-derived mesenchymal stem cell convergence rate reaches 85-90%, obtaining a cell culture supernate, carrying out membrane filtration, and collecting the filtrate; centrifugating the filtrate, collecting the centrifugal supernate, adding a 10w / v%-12w / v% polyethyleneglycol culture medium supernate solution into the centrifugal supernate according to the volume ratio of 1:1, sufficiently and uniformly mixing, and carrying out secondary centrifugation, wherein the finally obtained precipitate is the exosome. The method is convenient for taking materials, is easy for enrichment amplification culture, reutilizes the P2-P3 placenta-derived mesenchymal stem cell culture medium supernatant to obtain the exosome, and does not refer to the problem of medical ethics. In the exosome separation process, membrane filtration, centrifugation, polyethyleneglycol solution addition and other means are adopted to effectively increase the exosome enrichment, so the method has the advantages of short time consumption and low cost.

The invention is a Chinese medicinal preparation for curing male sterility, characterized by that it is prepared of the following raw materials: Membranous Milkvetch Root, largehead atractylodes Rhizome, Fuling, Liquoricee Root, White Peony Root, Prepared Rehmannia Root, Turmeric Root, Herba Epimedii, King Solomonseal Rhizome, Tuber Fleeceflower Root, Common Macrocarpium Fruit, Common Yam Rhizome, Desertliving Cistanche, Tortoisesshell Glue, Medicinal Indianmulberry Root, Rhizoma Curculiginis, Himalayan Teasel Root, Hairy Antler, Deerhorn Glue, Human Placenta, Tremolitum Tremolite,Tangshen Radix Codonopsis, Songaria Cynomorium Herb, Eucommia Bark, Fructus Rubi, Plantain Seed, wolfberry fruit, Schisandra, Chinese Dodder Seed, Glossy Privet Fruit, Papermulberry Fruit and Malaytea Scurfpea Fruit; Its form can be powder or soup and it can cure male sterility, nourish Yin, tonify kidney and strengthen Yang, build up constitution, promote the blood circulation, remove damp and blood stasis and promoting fluid, and has better curative effect but no toxicity and side effect.

The invention relates to a health-care product composition containing a placenta component and a preparation method thereof. The composition uses ganoderma lucidum polysaccharide and human placenta powder as basic effective components which can be formed together with at least one of vitamin E and vitamin C when necessary and pharmaceutically acceptable assistant components, wherein the weight ratio of the ganoderma lucidum polysaccharide to the human placenta powder expressed by amount of protein in the effective components is between 1 to 0.1 and 1 to 5, preferably between 1 to 0.1 and 1 to 1. Through tests, the composition not only can enhance immunological competence of human body, but also can delay senility, has remarkable fading effect on senile plaque or chloasma, and is remarkably superior to the prior homogeneous medicament and / or health-care products containing the placenta component on effect.

The invention relates to a traditional Chinese medicine composite in the field of Chinese herba preparations which nourish kidney yang. The technical scheme is that the composite consists of raw materials with the parts by weight as follows: 20 to 30 parts of ginseng, 10 to 20 parts of cinnamon, 20 to 30 parts of morinda offcinalis, 15 to 20 parts of dodder seed, 20 to 30 parts of human placenta, 10 to 20 parts of Chinese caterpillar fungus, 10 to 20 parts of cornu cervi pantotrichum, 20 to 30 parts of hippocampi, 20 to 30 parts of desertliving cistanche, 25 to 30 parts of rehmanniae vaporata, 10 to 20 parts of medicinal cyathula root, 20 to 30 parts of songaria cynomorium herb, 20 to 30 parts of short horned epimedium, 20 to 30 parts of astragalus hoangtchy, 15 to 20 parts of angelica, 20 to 30 parts of cortex eucommiae, 15 to 20 parts of licorice, 10 to 15 parts of radices saussureae, 20 to 25 parts of fructus lycii and 20 to 30 parts of cornus officinalis. The compatibility of the invention can tonifying qi and lifting yang, warm the interior and disperse the coldness, tonify the kidney and strengthen yang, encourage sperm and tonify marrow, promote blood circulation and remove blood stasis, and dispersing stagnated hepatoqi. The composite has very good curing and nourishing effects on impotence, spermatorrhea and frequent micturition which are caused by impotence due to deficiency of the kidney, softening of bones and muscles, insufficiency of kidney-yang and loss of essence and blood and can enhance the autoimmunity.

The invention belongs to the technical field of traditional Chinese medicine, particularly relates to a traditional Chinese medicine for treating cardiovascular and cerebrovascular diseases and a preparation method of the traditional Chinese medicine. The traditional Chinese medicine for treating the cardiovascular and cerebrovascular diseases is characterized being prepared from the following raw materials in parts by weight: 2 to 4 parts of radix panacis quinquefolii, 1 to 3 parts of dried human placenta, 5 to 7 parts of radix salviae miltiorrhizae, 5 to 7 parts of herba rhodiolae, 1 to 3 parts of schisandra chinensis fructus, 4 to 6 parts of head of angelica sinensis, 4 to 6 parts of Chuanxiong rhizoma, 3 to 5 parts of flesh of polygalae radix, 4 to 6 parts of hirudo, 4 to 6 parts of radix notoginseng, 4 to 6 parts of fried spina date seed, 7 to 9 parts of herba cistanche, 1 to 3 parts of saussurea involucrate, 3 to 5 parts of ganoderma lucidum spore, 2 to 4 parts of dendrobium officinale, 2 to 4 parts of grilled polygonum multiflorum, 4 to 6 parts of radix puerariae, 4 to 6 parts of rhizoma gastrodiae, and 9 to 11 parts of herba bidentis pilosae. Various herbs are matched to complement each other and to have functions for tonifying Qi, promoting blood circulation to remove blood stasis and activating main and collateral channels. The traditional Chinese medicine is widely used for treating the cardiovascular and cerebrovascular diseases.

The invention discloses an isolated culture method of human amnia mesenchymal stem cells, which comprises the following steps: carrying out human placenta amnia tissue acquisition, amnia tissue disinfection, washing, shredding and mixed collagenase digestion to obtain mesenchymal stem cells, and carrying out mesenchymal stem cell in-vitro culture. The method can obtain abundant mesenchymal stem cells, which have the advantages of uniform and stable phenotype and high multiplication capacity and have the multidirectional differentiation potential, from the human amnia tissues; and the culture medium in-vitro amplification is performed to purify the cells, thereby satisfying the quantity for cryopreservation or clinical use. The method has the advantages of short cell culture period and high repetitiveness. The stem cells obtained by the method have the phenotypes of CD105<+>, CD90<+> and CD73<+>.

The invention discloses traditional Chinese medicine mask powder for whitening and dispelling freckle, which has the advantages of little toxicity and capability of effectively achieving whitening, dispelling freckle and the like. A formulation for the mask powder comprises 3 to 10 grams of ginseng, 3 to 15 grams of rhizome polygonati, 6 to 15 grams of radix asparagi, 3 to 10 grams of rhizome typhonii, 3 to 10 grams of angelica dahurica, 6 to 15 grams of angelica, 3 to 10 grams of white stiff silkworm, 6 to 15 grams of fructus xanthil, 3 to 10 grams of bletilla, 6 to 15 grams of white gourd seed, 3 to 15 grams of gingko leaf, 3 to 10 grams of fragrant solomonseal rhizome, 3 to 10 grams of puncturevine, 3 to 10 grams of euphorbia lathyris, 1 to 6 grams of pearl powder, 1 to 6 grams of litharge, 3 to 10 grams of mint, 1 to 3 grams of human placenta, 3 to 6 grams of cinnamon, 3 to 10 grams of tasselblower, 3 to 10 grams of leucophanite, 1 to 3 grams of borneol, 10 to 30 grams of green bean powder, and 3 to 10 grams of honey-fried licorice root.

The invention discloses a preparation method of the acellular dried active amnion, comprising the following steps: (1) material processing: taking a human placenta which passes the detection of pathogen and obtained from a full term cesarean section, washing the human placenta with aseptic normal saline, removing blood components from the human placenta, blunt dissecting the amnion from the human placenta perfectly, striking off matrix components from the amnion substrate with a cell curet; (2) repeatly freezing and thawing; (3) washing; (4) freeze drying; and (5) disinfecting and sterilizing with gamma-ray. The method adopts the combination of repeat freeze-thaw method and using decontaminating agent to remove amnion cells, overcomes the defects of bad effects of removing cells by using freeze-thaw method alone, removes amnion cells effectively, keeps the integrity of collagen structure and basement membrane in tissue well, and keeps good mechanical property and no cytotoxicity.

The invention relates to a method for separating and culturing human chorionic mesenchymal stem cells, belonging to the technical field of biomedicine. The present invention obtains mesenchymal stem cells and in vitro culture and freezing of mesenchymal stem cells through the collection of human placental chorionic tissue, stripping of chorionic tissue, disinfection, washing, shredding, primary separation, and the isolated cells are related detection. The invention can separate and obtain a large number of mesenchymal stem cells with uniform and stable phenotype from human placental chorionic tissue, which meets clinical application standards through testing. The number of cells that can meet cryopreservation or clinical use can be obtained by culturing in vitro expansion.

The invention relates to a method for preparing anti-aging, antifatigue and essential qi-supplementing granules and capsules. The method comprises: mixing codonopsis pilosula, raidx astragali, mulberry, gordon euryale seed, scorch-fried white atractylodes, Indian buead, manyflower solomonseal rhizom, tuber of multiflower knotweed, wild soybean, Chinese magnoliavine fruit, fragrant solomonseal rhizome, raisin, human placenta, rehmannia glutinosa, south dodder seed, dwarf lilyturf tuber, root of rehmannia, lotus seed, dogwood, cervus elaphus linnaeus, radix glycyrrhizae preparata, Huai yam, stir-fried spiny jujube, dried longan pulp, salvia miltiorrhiza bunge and dark pum fruit; performing two times of extraction by decocting with 8 times of water and decocting with 6 times of water for 2 hours respectively; combining extracts for concentration; drying the extracts to make dry paste; grinding the dry paste obtained at two times into fine powder; adding milk powder, sucrose, amylin, edible essence and vegetable fat powder into the fine powder and mixing the mixture; adding starch serving as an auxiliary material; and making the resulting product into granules or filling the resulting product into hollow capsules to make capsules and bagging the capsules. The granules and capsules of the invention have the advantages of remarkable effect, simple administration, nice taste and toxic or side effect prevention.

A serum-free cryoprotectant includes: 8-15 v / v% of DMSO, 85-92 v / v% of a DMEM basic culture medium, and a nutritional additive. The nutritional additive includes: fibroblast growth factors, insulin, growth hormone, transferrin, bone morphogenetic protein 4, glutamine, sodium pyruvate, beta-mercaptoethanol, human epidermal growth factor, sodium selenite, and various amino acids and vitamins. The cryoprotectant is free of animal sourced serum and avoids pollution and risk of allergen, and has better clinical safety. The serum-free cryoprotectant is suitable for cryopreservation of human placenta sourced, umbilical cord sourced and cord blood sourced mesenchymal stem cells; compared with common serum cryoprotectants, perinatal mesenchymal stem cells preserved in the cryoprotectant have high cell survival rate after resuscitation, have excellent adherence growth status and maintain biological characters well.

The present invention provides nucleotide and amino acid sequences that identify and encode a new protein with homology to pregnancy-associated plasma protein-A (PAPP-A). We denote this protein PAPP-A2. The cDNA encoding PAPP-A2 was derived from human placenta. The present invention also provides for antisense molecules to the nucleotide sequences which encode PAPP-A2, expression vectors for the production of purified PAPP-A2, antibodies capable of binding specifically to PAPP-A2, hybridization probes or oligonucleotides for the detection of PAPP-A2-enoding nucleotide sequences, genetically engineered host cells for the expression of PAPP-A2, use of the protein to produce antibodies capable of binding specifically to the protein, methods for screening for pathologies in pregnant and non-pregnant patients that are based on detection of PAPP-A2 antigen in human body fluids or PAPP-A2-encoding nucleic acid molecules, use of the protein to screen for agents that alter the protease activity of PAPP-A2, use of the protein as a therapeutic target for such agents, and use of the protein as a therapeutic agent in relevant pathological states. Methods for screening for altered focal proliferation states in pregnant and / or non-pregnant patients, which include detecting levels of PAPP-A2, are also described. The present invention also provides the identification of a natural substrate of PAPP-A2, insulin-like growth factor binding protein (IGFBP)-5.

The invention discloses a preparation method of water soluble active collagen. The preparation method comprises the following steps: extraction of acid-soluble natural collagen; purification of acid-soluble natural collagen; preparation of a water soluble active collagen coarse product; and preparation of the water soluble active collagen. The invention further discloses an epidermis repair growth factor liquid containing the water soluble active collagen prepared by the preparation method. According to the technical field of the water soluble active collagen disclosed by the invention, wastes are turned to wealth, so that the value of umbilical cord and placenta is utilized and developed to the maximum extent. The water soluble active collagen is extracted from a healthy human placenta and / or umbilical cord, is easy to absorb and avoids immunoreaction caused by allogenic materials. The water soluble active collagen is free from external factors and does not contain chemical additives, so that the water soluble active collagen is closer to nature when in use, and the combined effect is best. By taking the water soluble active collagen serving as a main raw material can be matched with various cell factors and plant essences, so as to achieve the effects of repairing and regenerating skin, preventing sensitivity, diminishing inflammation, reducing wrinkles and lightening stains, avoiding adverse reaction and achieving good safety.

The invention discloses a drug for treating leucoderma and a preparation method thereof. The drug is prepared from gecko, human placenta, fructus psoraleae, prepared rehmannia root, pulp of cornus officinalis, dendrobium, radix astragali, pilose antler, fleece-flower root, glossy privet fruit, donkey-hide gelatin, tortoise-plastron glue, semen cuscutae, rhizoma polygonati, root of Angelicasinensis (Oliv)Diels, semen astragali complanati, raspberry, wolfberry, red ginseng, pseudo-ginseng, ganoderma atrum, radix salviae miltiorrhizae, stigma croci, long-nosed pit viper, tribulus terrestris, ligusticum wallichii, dahurian angelica root, divaricate saposhnikovia root, balloonflower root, tree peony bark, tuber fleeceflower stem, trogopterus dung, white-stiff silkworm, fruit of Chinese magnoliavine, katsumadai seed, medicated leaven, chicken's gizzard membrane, radish seed, bighead atractylodes rhizome, poria cocos, fructus amomi, herba siegesbeckiae, coix seed, black bean hull, esliptae herba, duckweed, motherwort, lithospermum, common clubmoss herb, periostracum serpentis, periostracum cicada, tamarix chinensis, native copper, Chinese-date, dried orange peel and madder. The drug does not damage the human body, realizes treatment on both symptoms and root causes and thoroughly cures leucoderma.

The invention relates to a Chinese medicinal decoction for treating leucocytopenia caused by chemotherapy for cancer, and also to the process for preparation, wherein the constituents include (by weigh ratio) root of American ginseng 20g, hairy deerhorn 30g, ganoderma lucidum 20g, astragalus root 30g, fruit of barbary wolf berry 20g, Chinese caterpillar fungus 20g, dry human placenta 20g, aniseed 10g, prepared aconite root 20g, notoginseng 10g, euphoria longan steud 20g, white atractylodes rhizome 10g, Ficus carica 20g, dried rehmannia root 20g, psoralea fruit 10g, aloe 10g, turtle shell 10g, ophiopogon root 10g, ligustrum japonicum 20g, Siberian solomonseal rhizome 10g, broomrape 10g, mongloian snakegourd 10g, root of herbaceous peony 10g, coix seed 10g, cornus officinalis 10g, rhubarb horsetails 5g, cape jasmine 5g, schisandra fruit 10g, licorice root 10g, lily bulb 5g.

The invention provides a sub-totipotent stem cell of a human placenta. The sub-totipotent stem cell of the human placenta is derived from stripped human placenta amnion, aiming at the characteristics of cells contained by an epithelial layer and a mesenchyme layer of the amnion, a step-by-step separation method is adopted, pollution of the sub-totipotent stem cell of the placenta by the mesenchyme layer of the amnion is reduced to the greatest extent, and the stemness of the sub-totipotent stem cell of the human placenta is improved effectively. The method has the characteristics of low cost, broad application prospect and capacity of supplying a large quantity of stem cell resources to clinic and research. The invention further provides a method for preparing the sub-totipotent stem cell of the human placenta from the human placenta amnion and constructing a stem cell bank.

The invention discloses a traditional Chinese medicinal composition for treating osteoporosis, which is prepared from the following raw materials in part by weight: 1 to 5 parts of prepared rehmannia root, 1 to 5 parts of cornus officinalis, 1 to 4 parts of antler glue, 1 to 4 parts of tortoise plastron, 1 to 4 parts of epimedium herb, 1 to 4 parts of prepared common monkshood daughter root, 1 to 4 parts of earthworm, 1 to 4 parts of cinnamon, 1 to 4 parts of seed of Chinese dodder, 1 to 4 parts of flastem milkvetch seed, 1 to 5 parts of medlar, 1 to 4 parts of dried human placenta, 1 to 4 parts of astragalus root, 1 to 4 parts of lushuigui, 1 to 4 parts of eucommia bark, 1 to 5 parts of yam, 1 to 5 parts of bighead atractylodes rhizome, 1 to 4 parts of ginger, 1 to 4 parts of pangolin scales, 1 to 4 parts of health hawthorn, 1 to 4 parts of massa medicata fermentata fujianensis, 1 to 5 parts of Chinese date, 1 to 5 parts of whole pericarpium trichosanthis, 2 to 4 parts of root of rehmannia, 2 to 4 parts of desertliving cistanche, 2 to 4 parts of cornua cervi pantotrichum, 2 to 4 parts of American gingseng, 2 to 4 parts of ground beetle, 2to 4 parts of rhizoma corydalis, 2 to 4 parts of sappan wood, 1 to 5 parts of black sesame, 2 to 4 parts of cordyceps, 2 to 4 parts of medicinal indianmulberry root, 2 to 4 parts of common cnidium fruit and 2 to 4 parts of prepared root of fleeceflower. The traditional Chinese medicine has the effects of tonifying kidney and invigorating the circulation of blood, and strengthening sinews and bones and has obvious treatment effect on the osteoporosis.

Compositions containing purified collagen biomaterial derived from tissues, for example, insoluble amnion, soluble amnion, soluble chorion of the human placenta, are provided. The collagen compositions can be used to promote wound healing, promote tissue regeneration, prevent or reduce scarring, reduce local inflammation, minimize tissue rejection, promote graft integration. Methods for using the collagen composition as a biomaterial implant for dermal filling, skin grafting, and hair transplantation are also provided.

The invention relates to an age-reducing skin care product aiming at cell gene regeneration and repair. The age-reducing skin care product consists of the following raw materials in percentage by weight: 8-20 percent of human placenta extract, 4-10 percent of magnesium ascorbyl phosphate, 1-3 percent of acetyl hexapeptide-8, 1-3 percent of palmitoyl oligopeptide, 1-3 percent of palmitoyl tetrapeptide-3, 1-3 percent of tocopherol, 0.05-2 percent of eryngium maritimum extract, 0.05-3 percent of sodium hyaluronate, 3-5 percent of xanthan gum, 0.30 percent of bi(hydroxymethyl) imidazolidinyl urea, 0.20 percent of allantoin, 0.10 percent of EDTA tetrasodium, 0.03 percent of sodium pyrosulfite, 11.00 percent of butanediol and the balance of deionized water. Polypeptide tightening factors of a proper ratio such as acetyl hexapeptide-8, palmitoyl oligopeptide and palmitoyl tetrapeptide-3 are adopted and are combined with the human placenta extract and eryngium maritimum extract, and the product has the effects of tightening the skin, increasing the skin elasticity, preventing wrinkles and delaying skin aging.

We have discovered a protein in humans, herein referred to as collagen like gene (CLG) product (SEQ ID NOS: 12 and 13), that is expressed in human prostate cancer and breast cancer cell lines but not in normal adult, placenta, lung, liver, skeletal muscle, kidney or pancreas tissues. We have also discovered that the level of CLG mRNA expression correlates positively with the metastatic potential of the cancer cell lines tested.

The invention provides a Chinese traditional medicine preparation for effectively curing osteoporosis, which is composed of the following Chinese traditional medicines: Prepared rhizome of Adhesive Rehmannia, Herb of Shorthorned Epimedium, Dried Human Placenta, Oriental Waterplantain Rhizome, Drgonsbones, Herb of Tuberculate Speranskia, glue of tortoise plastron, frankincense, eucommia bark, rhizoma drynariae, polyporus lucidus, Salvia miltiorrhiza, achyranthes, medlar, spatholobus stem, safflower, rhubarb, Tuckahoe, Rhizome of Skunk Bugbane, dried orange peel, myrrh and Rhizome of Common Anemarrhena. According to Chinese extensive and profound traditional medical theory, the medicine preparation is made of more than 20 kinds of Chinese traditional herbs which are seriously selected through clinical experiments to validate repeatedly; the medicine preparation has fine curative effect for curing various clinical symptoms of osteoporosis, and the Chinese traditional medicine has the advantages that the production is convenient, the effect is immediate after taking the medicine, and the medicine has no side effects and bad reactions after using.

The invention discloses a method for serum-free domestication and culture of human mesenchymal stem cells. According to the method, human placenta-derived mesenchymal stem cells are allowed to adapt to an in-vitro serum-free culture system, and cell density and cell viability similar to the cell density and cell viability of serum culture are obtained; and compared with conventional culture methods for human mesenchymal stem cells, the method provided by the invention is more economical and effective. With the method, the expression levels of bFGF, PDGF and VEGF associated with blood vessel growth are increased compared with undomesticated cells. The domesticated cells can be prepared for preparing stem cell preparations for intravenous injection; and active factor components secreted by the domesticated cells can be used as components of a mask, an eye mask and a hair mask.

The invention discloses a culture medium for expanding human mesenchymal stem cells, which comprises a basic medium and an additive added to the medium, and the additive includes the following components at final concentrations: linoleic acid 2‑10 μL / mL, human Source AB type serum 5‑15 μL / mL, human transferrin 5‑15 μg / mL, etc. Using the culture medium to expand human mesenchymal stem cells includes the following steps: (1) isolating human mesenchymal stem cells from umbilical cord or placenta, or isolating mononuclear cells from bone marrow blood, umbilical cord blood or placental blood, and placing them in the culture medium (2) Place the cells separated and purified in step (1) into fresh medium for subculture. The invention adopts the culture medium of specific components and the expansion method, which can effectively speed up the cell expansion speed, shorten the expansion time, maintain the uniform shape of the cells after passaging, and reduce their differentiation, thereby ensuring the safety of human mesenchymal stem cells sex.