The invention discloses a culture medium good in hematopoietic stem cell adherence. The culture medium comprises an RPMI-1640 culture medium, 5-8mug/L of hyaluronic acid, 3.2-3.8g/L of trehalose, 20-30mg/L of ethanolamine, 0.7-1g/L of potassium bicarbonate, 6-9mug/L of methionine chelate selenium, 2-5mug/L of beta-mercaptoethanol, 2-3g/L of transferrin, 200-260mg/L of stem cell factors, 50-80mg/L of placental growth factors, 30-50mg/L of fibroblast growth factors, 80-100mg/L of glutathione, 80-110mg/L of vitamins and 180-220mg/L of plant extracts. The culture medium is good in cell adherence, rapid in proliferation rate, pathogen-resistant microbial action of the cultural medium is improved, components of animal origin are avoided, residues or pollution cannot be caused, and cost of the culture medium is lowered.