Placental growth factor loaded nano-particle, as well as preparation method and application thereof

A technology of placental growth factor and nanoparticle, which is applied in the direction of pharmaceutical formulations, medical preparations containing no active ingredients, and medical preparations containing active ingredients. It can solve the problems of good slow-release effect and high encapsulation rate, and achieve slow-release Good effect, good stability, and not easy to protein denaturation

Active Publication Date: 2014-10-29
SHANGHAI MODERN PHARMA ENG INVESTIGATION CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the defect that the preparation method of placenta-carrying growth factor nanoparticles in the prior art cannot simultaneously realize good slow-release effect and high encapsulation efficiency, and provide a new placenta-carrying growth factor nanoparticle and its Preparation method and application, the placenta growth factor-carrying nanoparticle has good slow-release effect, high encapsulation efficiency and low toxicity, and can stably, continuously and effectively deliver the placenta growth factor to the myocardial infarction area

Method used

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  • Placental growth factor loaded nano-particle, as well as preparation method and application thereof
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  • Placental growth factor loaded nano-particle, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Preparation of the inner water phase: prepare 0.15 mg / mL PLGF aqueous solution, take 100 μL (0.1 mL) as the inner water phase (W1), and the concentration is the mass ratio of the placental growth factor to the volume of the inner water phase.

[0053] (2) Preparation of the oil phase: Dissolve 10 mg of PLGA (viscosity average molecular weight 60,000, lactic acid, glycolic acid block ratio 50:50) in 0.5 mL of ethyl acetate to prepare a 20 mg / mL solution as the oil phase ( O); said percentage is the mass of PLGA and the volume ratio of ethyl acetate.

[0054] (3) Add W1 dropwise to O, and ultrasonically disperse. The power of ultrasonic dispersion is 200w, and the time is 2min, forming a water-in-oil (W1 / O) emulsion.

[0055] (4) Add the (W1 / O) type emulsion in step (3) to 2mL of 19% Pluronic F-68 aqueous solution (i.e. the outer aqueous phase), the percentage is Pluronic F-68 The weight percent of the external water phase; under ice bath conditions, use a dispersing...

Embodiment 2

[0061] (1) Preparation of the inner water phase: prepare 0.15 mg / mL PLGF aqueous solution, take 100 μL as the inner water phase (W1), and the stated concentration is the mass ratio of the placental growth factor to the volume of the inner water phase.

[0062] (2) Preparation of the oil phase: Dissolve 5 mg of PLGA (viscosity average molecular weight 60,000, lactic acid, glycolic acid block ratio 50:50) in 0.5 mL of ethyl acetate, and prepare a 10 mg / mL solution as the oil phase (O); said percentage is the volume ratio of the mass of PLGA to ethyl acetate.

[0063] (3) Slowly add W1 to O, and ultrasonically disperse. The power of ultrasonic dispersion is 200w, and the time is 1min, forming a water-in-oil (W1 / O) emulsion.

[0064] (4) Add the (W1 / O) type emulsion in step (3) to 2mL of 15% Pluronic F-68 aqueous solution (i.e. the outer aqueous phase), the percentage is Pluronic F-68 in the outer The weight percent of the water phase; under ice bath conditions, the high-speed sh...

Embodiment 3

[0069] (1) Preparation of the inner water phase: prepare 0.20 mg / mL PLGF aqueous solution, take 100 μL as the inner water phase (W1), and the concentration is the mass ratio of the placental growth factor to the inner water phase.

[0070] (2) Preparation of the oil phase: Dissolve 10 mg of PLGA (viscosity average molecular weight 60,000, lactic acid, glycolic acid block ratio 50:50) in 0.5 mL of ethyl acetate to prepare a 20 mg / mL solution as the oil phase (O); said percentage is the volume ratio of the mass of PLGA to ethyl acetate.

[0071] (3) Slowly add W1 to O, and ultrasonically disperse. The power of ultrasonic dispersion is 200w, and the time is 1min, forming a water-in-oil (W1 / O) emulsion.

[0072] (4) Add the (W1 / O) type emulsion in step (3) to 3mL of 10% Pluronic F-68 aqueous solution (i.e. the outer aqueous phase), the percentage is Pluronic F-68 in the outer The weight percent of the water phase; under ice bath conditions, the high-speed shear emulsification of ...

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Abstract

The invention discloses a placental growth factor loaded nano-particle, as well as a preparation method and application thereof. The method comprises the following steps: 1, dispersing an internal water phase (a water solution containing placental growth factors) in an oil phase (an organic solution containing poly(lactic-co-glycolic acid) and an organic solvent) to form colostrum, wherein the polarity of the organic solvent is 4.0-6.0, and the ratio of the internal water phase to the oil phase is (1:3)-(1:10); 2, emulsifying the colostrum in an external water phase (a water solution containing a hydrophilic nonionic surfactant with HLB of 8-18) to form multiple emulsion, wherein the ratio of the oil phase and the external water phase is (1:3)-(1:6); 3, mixing the multiple emulsion and an expanded phase (water or a water solution containing a stabilizing agent), removing the organic solvent and the stabilizing agent, and lyophilizing, wherein the ratio of the external water phase to the expanded phase is (1:5)-(1:15). The placental growth factor loaded nano-particle has a good sustained-release effect, the preparation method can be used for reducing protein degeneration and improving encapsulation efficiency and has a good stability, the organic solvent cannot be dichloromethane or other solvents, and the reaction toxicity can be reduced.

Description

technical field [0001] The invention relates to placenta-carrying growth factor nanoparticles, a preparation method and application thereof. Background technique [0002] Acute myocardial infarction (AMI) is a disease in which the coronary artery is partially or completely occluded due to coronary artery disease, and the blood supply is sharply reduced or interrupted, resulting in myocardial necrosis due to persistent and severe ischemia and hypoxia. Acute myocardial infarction is a common cardiovascular disease with sudden onset, many complications and high mortality. The current main treatment methods for acute myocardial infarction include drug therapy, heart transplantation, left ventricular implantable assistance, etc. Due to the limitations of organ donation matching and the expensive and complicated process of implanting left ventricular assist devices, drug therapy has become the main treatment method. A new type of drug therapy is to inject placental growth factor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K47/34A61K9/19A61K9/113A61P9/10
Inventor 何军连锋毛立丽路喆鑫刘洁卞玮潘伟祥
Owner SHANGHAI MODERN PHARMA ENG INVESTIGATION CENT
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