21results about How to "Reduce denaturation" patented technology

The invention relates to the technical field of medical examination, in particular to a method for rapidly detecting a neutrophil extracellular trapping net. The method comprises the following steps: (1) taking a human whole blood sample, adding a hemolytic agent, then adding a mixed fluorescent antibody staining solution, shaking and uniformly mixing, and standing and incubating at room temperature; (2) centrifuging after incubation in the step (1), absorbing and discarding a supernatant, washing with a washing solution, centrifuging, absorbing and discarding the supernatant; (3) adding a washing solution to resuspend the cells; and (4) detecting by adopting a flow cytometer. The detection method is good in stability, high in repeatability and high in fluorescence intensity, and can be used for auxiliary diagnosis of diseases.

The present invention relates to a food-grade cross-linking agent with high permeability and its application. The food-grade cross-linking agent with high permeability is a pre-cross-linked oligosaccharide containing two reducing ends in a single molecule obtained by treating pre-cross-linked starch, and can improve substrate performance by reacting with amino substrates to generate Maillard reaction and acetal or hemiacetal reaction with hydroxyl substrates. The cross-linking agent is food-grade, not only has very high safety, but also has the advantages of simple preparation and strong cross-linking activity; in addition, the cross-linking agent also has high permeability, and can significantly improve the stability of cross-linked substrates, especially bulk substrates, when cross-linked.

The invention provides a tissue slice patch preparation method based on a tissue chip. The tissue slice patch preparation method comprises the following steps: mixing raw materials to prepare a receptor wax block, collecting a tissue core and transferring the tissue core to the receptor wax block to form a fused wax block, cooling the fused wax block, storing the fused wax block, and carrying out operations of pre-cooling correction, slice unfolding and slice baking. According to the tissue chip-based tissue slice patch preparation method, the tissue chip can be better protected, and the integrity of the tissue chip is ensured.

The invention discloses a high-speed vacuum centrifugal concentration drying system and method, and the system comprises a centrifugal unit which is characterized in that a motor drives a centrifugal tube rack to rotate at a rotating speed of at least 1400 rpm, and a sample is fixed in a centrifugal tube through centrifugal force; according to the vacuum system, a vacuum pump is connected to the interior of the centrifugal unit through a vacuum pipeline and used for forming a low-pressure environment in the centrifugal unit and monitoring and adjusting the air pressure in the centrifugal unit in real time through a vacuum control unit; according to the heating unit, a heating piece is tightly attached to the outer surface of an inner container of the centrifugal unit and used for providing heat to make up energy consumed by solvent evaporation, and a temperature control system monitors the temperature of the sample and the heating piece in real time through a temperature sensor and automatically adjusts power output of the heating piece so as to maintain a preset temperature curve. The invention provides an efficient and reliable solution for concentration and drying of samples by comprehensively utilizing high-speed centrifugation, vacuum technology, accurate heating and motor rotating speed control.

This invention discloses a low-temperature desolventizing machine and its usage method, relating to the field of grain and oil deep processing technology. The low-temperature desolventizing machine includes a machine body and a steam-water integrated utilization device. The machine body includes a cylinder and a rotating mechanism. The cylinder includes a front end cover and a rear end cover located at its front and rear ends. The cylinder also includes an inner cylinder, which is equipped with a feed inlet, a front settling tank, a solvent gas inlet, a rear settling tank, and a discharge outlet. An outer jacket is provided outside the inner cylinder, comprising an annular jacket and multiple flow-guiding jackets. The steam-water integrated utilization device is connected to the flow-guiding jackets and the annular jacket via pipelines and can provide hot water circulation and flash steam heating to the inner cylinder. This invention uses hot water and flash steam instead of steam heating, changing the traditional A-cylinder / B-cylinder desolventizing method, and solving the problems of high residual solvent content, low nitrogen solubility index, dark color, high powder content, and large moisture loss in low-temperature meal, greatly reducing solvent and steam consumption.

To make it possible to place a particle analysis device in a limited space around a line and to maintain a short route for transporting the samples from the line, the particle analysis device 100 of the present claimed invention is configured so as to comprise a flow cell (C) through which a sample containing particles q12flows, a light source 10 that irradiates light to the particles in the flow cell (C), a photodetector 20 that detects secondary light from the particles, and an arithmetic circuit 30 that calculates an autocorrelation function from a light intensity signal output by the photodetector 20 and analyzes the particles contained in the sample using the autocorrelation function. The particle analysis device 100 is so configured that an optical system unit U1 including the flow cell (C) and the light source 10, and a control unit U2 including the photodetector 20 and the arithmetic circuit 30 are separately provided from each other and are connected through a light guide member L1 that guides the secondary light to the photodetector 20.

The invention relates to the technical field of medicines, and discloses a preparation method and application of an alcohol extract of root tuber of pilose asiabell. The preparation method comprises the following steps: crushing dried tuberous roots of cayenne pepper, sieving, weighing cayenne pepper powder, adding 95% ethanol, soaking and extracting at room temperature for 2 days, stirring once within 2 hours, once stirring for 30 minutes, performing suction filtration after soaking to obtain ethanol leaching liquor, soaking and extracting filter residues for 2 times, merging the ethanol leaching liquor, transferring into a rotary vacuum concentrator, and concentrating to obtain the cayenne pepper pure extract. Concentrating and drying to obtain a maozhou root tuber alcohol extract; experimental verification shows that the prepared pure extract of the sauced head has the effect of relieving the fatty liver, so that the pure extract of the sauced head can be used for preparing the medicine for relieving the fatty liver.

The invention belongs to the technical field of molecular biology, and particularly relates to a digestion method, a passage method and a cryopreservation method of mouse mononuclear macrophages, and the method comprises the following steps: adding a phosphate buffer solution with the temperature of 1-4 DEG C into a culture for culturing the mouse mononuclear macrophages for digestion; digesting at the temperature of 1 to 4 DEG C for 5 to 10 minutes; the concentration of the phosphate buffer solution is 0.01 M to 0.015 M; the pH value of the phosphate buffer solution is 7.2-7.4. According to the method disclosed by the invention, the success rate of RAW cell passage and cryopreservation is obviously improved, the possibility of cell differentiation, denaturation, rupture and the like caused by pancreatin digestion, cell scraping and the like is reduced, and the efficiency of RAW cell experiments is effectively improved.

A method for improving stability of a black corn anthocyanin-protein compound comprises the following steps: adding a stabilizer which is glucose or maltodextrin into a black corn anthocyanin-protein compound solution; when the stabilizer is glucose, the concentration of the glucose in the solution is 1-5%; when the stabilizer is maltodextrin, the concentration of the maltodextrin in the solution is 1-3%. According to the invention, maltodextrin or glucose is adopted as a stabilizer, so that the stability of the black corn anthocyanin-protein compound is improved. The maltodextrin is combined with the protein part or the anthocyanin part in the compound, so that the structure of the compound is more compact and stable, dissociation and denaturation of the compound in the external environment are reduced, the combination state between the anthocyanin and the protein is kept, the stability of the anthocyanin is indirectly protected, meanwhile, the stability of the protein structure is facilitated, and the stability of the protein structure is improved. Therefore, the protection effect of the protein on the anthocyanin can be better exerted.

The preparation method comprises the following steps: inoculating activated yeast thalli into a liquid seed culture medium, culturing in a shaking table, inoculating into a fermentation culture medium, culturing, and centrifugally washing to obtain the yeast thalli; preparing the saccharomycetes into a suspension solution, and carrying out heat treatment to obtain a precipitate; preparing the precipitate into a suspension, adding 5-50U / mL glucanase and 5-50U / mL cellulase at the temperature of 5.0-7.0 DEG C and the temperature of 30-60 DEG C, and performing enzymolysis to obtain a first enzymatic hydrolysate; adding a nonionic solubilizer into the first enzymatic hydrolysate at a certain pH value, and carrying out heat preservation treatment at a certain temperature to obtain a second enzymatic hydrolysate; and carrying out solid-liquid separation, washing and drying on the second enzymatic hydrolysate to obtain the high-purity yeast protein. According to the preparation method, the possibility of protein denaturation is reduced, and the final yeast protein product is low in dietary fiber content, high in branched chain amino acid content and high in protein content and has good nutritional value and processing characteristics.

The invention relates to the technical field of food processing, in particular to a preparation method and application of hippocampal peptide. The preparation method of the hippocampal peptide comprises the following steps: performing ultrasonic treatment on a degreased hippocampal powder aqueous solution, and performing vacuum cooking to obtain a first solution; adding hydrolase into the first solution for enzymolysis to obtain a second solution; carrying out alcohol precipitation reaction on the second solution to obtain a third solution and a precipitate; purifying the precipitate to obtain a second hippocampal peptide initial solution; and mixing the hippocampal peptide first initial solution and the hippocampal peptide second initial solution to obtain the hippocampal peptide. And ultrasonic treatment is matched with low-temperature vacuum cooking, so that the hydrolysis degree of the degreased hippocampus powder is improved, the enzymolysis efficiency is improved, and the extraction effect of hippocampus peptide is improved.

The invention discloses a bergamot pear polygalacturonase inhibitory protein extraction and purification method, which comprises: 1, crushing bergamot pears to obtain crushed bergamot pears; 2, precipitating the filtered crushed material by using an ammonium sulfate solution; 3, dissolving the precipitate with a buffer solution, and performing dialysis and primary purification to obtain a crude extract; and 4, purifying the crude extract by using a gel filtration chromatography column to obtain a purified extract, and drying to obtain the traditional Chinese medicine composition. The natural bergamot pear fruits are used as raw materials, and the method does not depend on chemical synthesis or genetic engineering technology, and accords with green, environment-friendly and sustainable development concepts; the extraction and purification process comprises multi-step purification processes such as filtration, precipitation, dissolution, dialysis and filter membrane filtration, and conventional laboratory technologies such as filter paper filtration, centrifugation and dialysis are used, so that the whole process is easy to implement and repeat, the feasibility and the reliability of experiments are improved, and the cost is reduced. The purity, the biological activity and the extraction rate of the extract are remarkably improved.

The invention discloses an extraction and activity protection method for functional components in an eggshell membrane. The method comprises the steps of raw material pretreatment and cryogenic superfine grinding, ultrasonic-assisted swelling, compound enzyme step-by-step targeted extraction, separation and purification, multi-source activity protection and the like. The method has the remarkable effects that the method is realized by an integrated technology of copious cooling superfine grinding, ultrasonic assistance, compound enzyme step-by-step targeted extraction and multi-element activity protection, the extraction efficiency of functional components is extremely high, the product activity is excellent, the molecular weight distribution of the extracted collagen is concentrated, and the collagen can be effectively extracted by virtue of a multi-source activity protection measure. The industrial problem that active ingredients are prone to inactivation is systematically solved, the product can be applied to different application scenes such as health care products, cosmetics and biological medicine, and high-value utilization of agricultural waste is achieved.

The invention relates to a detection material for detecting a GABABR antibody and a preparation method of the detection material. The detection material for detecting the GABABR antibody comprises the following components: an antibody detection plate, a working solution, PBS (Phosphate Buffer Solution), a fluorescent secondary antibody and a mounting medium, by introducing the composite material of the modified trehalose, the chitosan and the polyvinyl alcohol to the surface of the glass slide, the adhesive force of the antigen protein is remarkably improved by adopting the technical scheme. The modified trehalose not only provides higher surface activity, but also forms a stable binding layer by optimizing the interface action of the antigen and the carrier, thereby remarkably reducing the loss of the antigen protein caused by washing and operation steps in the detection process. According to the design, the problem of insufficient antigen fixing force in the prior art is solved, the effective concentration of antigen protein is ensured, and the detection sensitivity and the result reliability are improved.

The invention relates to the field of sampling detection, and discloses a sampling device for collecting a dense gas sample, the sampling device comprises a communicating pipe, a sampling pipe, a buffer tank and a sampler, one side of the buffer tank is communicated with the communicating pipe, a pressure valve is arranged in the communicating pipe, and the other side of the buffer tank is communicated with the sampler through the sampling pipe; the sampler comprises a tank body part and a tank opening part arranged on the tank body part, a piston body is arranged in the tank body part in a sliding mode, the bottom of the tank body part is an opening, the sampler further comprises a bottom plate detachably connected with the opening in the bottom of the tank body part, and the two ends of a first spring abut against the piston body and the bottom plate respectively. The sampling device provided by the invention can be used for rapidly and temporarily storing and sampling dense gas.

This invention relates to the field of ice cream processing technology, specifically providing a coated ice cream and its preparation method. The coated ice cream provided by this invention uses mayonnaise as the coating. By controlling the mayonnaise production process and viscosity, this invention ensures that the mayonnaise is evenly dispersed after being coated onto the ice cream surface, reducing protein denaturation caused by uneven mixing. The addition of stabilizers improves the emulsification properties of the coated ice cream product and enhances the hydrogen bond strength between proteins and water. The coated ice cream product obtained by this invention exhibits good melt resistance after being coated with mayonnaise, has a uniform coating thickness without obvious fat particles, does not crack during its shelf life, and is both nutritious and delicious.

The present invention provides a cryopreservation solution, a preparation method and a use method for kidney organoids, and relates to the field of cell and tissue engineering technology. The cryopreservation solution includes the following components by weight: 4.7 to 5.3 parts of cell cryopreservation solution; 3.5 to 4.5 parts of KOSR serum substitute; 0.8 to 1.2 parts of improved culture medium; wherein the improved culture medium includes RPMI 1640 culture medium and L-alanyl-L-glutamine, and the weight of RPMI 1640 culture medium is 3.5 to 4.5 times that of L-alanyl-L-glutamine. KOSR serum substitute and improved culture medium are added to CS10 cell cryopreservation solution to dilute the concentration of dimethyl sulfoxide, so that the dimethyl sulfoxide in the cryopreservation solution reaches the required minimum concentration, which not only reduces the formation of ice crystals, but also avoids the cytotoxicity caused by the excessively high concentration of dimethyl sulfoxide, thereby reducing the damage of kidney organoids.

The invention relates to a preparation method of an isavuconazole sulfate preparation, and belongs to the technical field of medicament preparation, by adding a dissolution-promoting stabilizer prepared from a mixture of water-soluble cellulose and chitosan, the solubility of isavuconazole sulfate in the preparation is improved, and rapid dissolution and absorption of a medicament in a body are facilitated, so that the bioavailability is improved. And mixing and unfreezing treatment are carried out at the low temperature of 0-10 DEG C, so that the stability of active ingredients of the medicine is favorably maintained, degradation or denaturation caused by high temperature is reduced, and the purity and curative effect of the product are ensured. Through pre-freezing and multiple unfreezing treatments and in combination with filter membrane filtration, impurities and particles in the solution can be effectively removed, and the clarity and purity of the preparation are improved. Then moisture is rapidly removed at low temperature, active ingredients and stability of the medicine are reserved, and meanwhile damage of high temperature to the medicine is avoided.

The utility model discloses a fermentation tank for collagen preparation, which comprises a mounting rack and a fermentation tank, the fermentation tank is rotatably connected with the mounting rack through a mounting shaft, the mounting shaft is sleeved with a gear, a rack is arranged in the mounting rack and is slidably connected with the mounting rack through a slider, a crank is rotatably mounted in the mounting rack, and the crank is connected with the mounting rack through a slider. A connecting rod is rotatably mounted on the sliding block, a mounting cover is fixedly mounted above the fermentation tank, the crank rotates and is matched with the connecting rod to convert the rotating motion of the crank into the reciprocating rectilinear motion of the rack driven by the sliding block on the mounting frame, and the rack is driven by the sliding block to move on the mounting frame in the reciprocating rectilinear motion process. The mounting shaft drives the fermentation tank to slowly swing left and right on the mounting frame by matching with the gear meshed with the mounting shaft, so that the fermented collagen raw materials in the fermentation tank are uniformly mixed, and the situation that the fermentation integrity of the raw materials in the fermentation tank is damaged by shearing force generated by traditional stirring, and consequently, the product denaturation or function loss is caused is reduced.

The invention relates to a kit for detecting an AChR antibody and a preparation method thereof. The kit for detecting the AChR antibody comprises an antibody detection strip, a working solution, PBS (Phosphate Buffer Solution), a fluorescent secondary antibody and a mounting medium, wherein the antibody detection plate is a glass slide on which a GABABR antigen and modified chitosan are applied; the modified chitosan is chitosan modified by hyaluronic acid and gamma-polyglutamic acid. By using the modified chitosan, the binding force between the antigen protein and the carrier is obviously enhanced. Chitosan has good biocompatibility, through synergistic modification of hyaluronic acid and gamma-polyglutamic acid, the surface of chitosan shows higher hydrophilicity and stronger charge adsorption capacity, the chitosan can be more stably combined with antigen protein, the falling risk of the antigen protein in the washing and multi-step reaction process is remarkably reduced, and the anti-tumor property of the antigen protein is improved. Therefore, the effective utilization rate and the reaction efficiency of the antigen in detection are improved.

The invention relates to a process for removing histamine and reducing loss of phospholipase A2 (PLA2) and melittin in bee venom at the same time.