47results about How to "Good biological activity" patented technology

The invention discloses a bioactive porous ceramic tubular bar material as well as a preparation method and application thereof. The bioactive porous ceramic tubular bar material comprises a non-biodegradable calcium magnesium silicate porous ceramic tube and a biodegradable calcium magnesium silicate modified layer, wherein the biodegradable calcium magnesium silicate modified layer is modified by calcium magnesium silicate, subjected to sintering treatment and then cover the pore channel wall of the porous ceramic tube. The preparation method comprises the following steps: performing mixing treatment on tetraethoxysilane, calcium salt, magnesium salt, nitric acid and trace element inorganic salt or acid to obtain hydrogel; putting the pre-built calcium magnesium silicate porous ceramic tube into the hydrogel, dipping, sucking, ageing, drying, calcining and cooling to obtain the bioactive porous ceramic tubular bar material. The tubular bar material provided by the invention can decompress a femoral head necrosis medullary core for a long time, can significantly improve vascularization and nutrient transmission of a necrotic area and can promote new bone regeneration of bone injury; the tubular bar material has excellent bioactivity and has application value in femoral head necrosis and defected bone remodeling of a large bone.

The preparation process of sintered porous titanium artificial bone with bioceramic coating belongs to the field of biomedicine engineering technology. The preparation process includes stereo weaving of titanium metal fiber to form bionic structure model simulating human body's bone trabecula, pre-pressing formation and vacuum sintering to prepare porous titanium artificial bone, sol-gel process to form gradient and composite coating on the surface of the porous titanium artificial bone. The present invention can protect titanium metal skeleton, avoid titanium ion dissociation and make the artificial bone possess biological characteristic, and may be used in the clinical repair of large bone defect.

The invention relates to an application of sargassum graminifolium polysaccharide extract in improvement of intestinal flora and prevention and treatment of diabetes. Concretely, the invention provides a sargassum graminifolium polysaccharide extract. A preparation method of the sargassum graminifolium polysaccharide extract comprises the following steps: drying, and crushing, so that sargassum graminifolium dry powder is obtained; carrying out ethanol reflux extraction, so that dry and defatted sargassum graminifolium powder is obtained; extracting the dry and defatted sargassum graminifolium powder by utilizing microwave and taking water as a solvent; separating and purifying extracting solution with a polyethyleneglycol/inorganic salt or ethanol/inorganic salt aqueous two-phase system; and collecting in inorganic salt bottom phase, filtering and concentrating by adopting an ultrafiltration membrane, and drying, so that the sargassum graminifolium polysaccharide extract is obtained. The sargassum graminifolium polysaccharide extract has high purity and good biological activity, and animal experiments verify that the intestinal flora can be obviously improved, and immunity of mouse can be obviously improved, so that the sargassum graminifolium polysaccharide extract can be used for preparing a medicine, health product or food which can be used for improving the intestinal flora and preventing and treating metabolic diseases such as diabetes.

The invention discloses a regulation and control method of mechanical strength of natural biologic material products. A final product is obtained by the treating steps of freezing and drying, compression, crosslinking and secondary drying on natural biologic material solution. The treating method adopts the controllable compression step for a primary product after freezing and drying, thereby controlling the density of a porous material product, reducing the porosity of the porous material product and controlling the mechanical performance of the final product by the step. Meanwhile, the adopted method does not influence the biologic performance of the initial natural biologic material, thereby still keeping better biologic activity of the final product.

The invention relates to oat peptide milk and a preparation method thereof. The oat peptide milk contains oat peptide liquid, fresh milk, cane sugar, emulsifying agent and stabilizing agent, wherein the content of protein in the oat peptide milk is 2.5-4 percent by weight, the content of the cane sugar in the oat peptide milk is 5-7 percent by weight, the content of the emulsifying agent in the oat peptide milk is 0.02-0.10 percent by weight and the content of the stabilizing agent in the oat peptide milk is 0.04-0.12 percent by weight. The preparation method comprises the steps of: baking oats for 10-30min at 100-125 DEG C, adding water and passing through a colloid mill to obtain oat slurry; adding composite hydrolase and alkali protease into the oat slurry for enzymolysis; when the degree of hydrolysis of the oat slurry reaches 6-12 percent, terminating enzymolysis reaction; conducting centrifugal separation to obtain liquid supernatant which is oat peptide liquid; and evenly mixing the oat peptide liquid, the fresh milk, the cane sugar, the emulsifying agent and the stabilizing agent with water, homogenizing, canning and sterilizing to obtain the oat peptide milk. The preparation method disclosed by the invention is simple, the content of protein in the provided oat peptide milk is high and stable and the flavor of the oat peptide milk is good.

The invention discloses a titanium-hydroxylapatite gradient coating and preparation method and application thereof. The coating of the invention takes matrix as basement and comprises the following layers from inside to outside: a titanium powder layer, a titanium powder and hydroxylapatite mixed layer, wherein the volume ratio of the two is 6:4, a titanium power and hydroxylapatite mixed layer, wherein the volume ratio of the two is 4:6, and a hydroxylapatite layer. The coating of the invention is sprayed by low pressure plasma spraying technology and can be used for treating titanium alloy implant surface. The coating of the invention has high stability and favourable retention of bioactivity and can reduce coefficient of thermal expansion and elastic modulus difference between coating material and implant, so that residual stress of coating is reduced and bonding strength with the implant is improved; and the coating of the invention is sprayed by applying plasma technology, thus poriness of the coating can be reduced and bonding strength with the implant can be improved.

The invention relates to a recombinant human type III collagen having a functional structure and a prokaryotic expression method thereof. The nucleotide sequence of an encoding gene of the human typeIII collagen is shown in SEQ ID NO:3, and through coexpression with hydroxylase Hy726 with a gene sequence shown in SEQ ID NO:4, the recombinant human type III collagen with the tertiary helical structure is obtained. The protein has better stability and functionality, and the biological activity is more comparable to biological activity of a natural human type III collagen.

The invention discloses a hyaluronic acid derived maytansine prodrug, a preparation method thereof and application of maytansine prodrug in preparation of tumor target treatment medicines. The maytansine prodrug comprises a hyaluronic acid main chain and a maytansine side chain, wherein maytansine and hyaluronic acid are connected by a disulfide bond, and the hyaluronic acid has a molecular weight of 7-500kDA; and the maytansine content in the hyaluronic acid derived maytansine prodrug is 10-50 percent. The hyaluronic acid derived maytansine prodrug is amphipathic, and can be self-assembled in an aqueous solution to form a nano-drug, the outer hydrophilic layer is formed by hyaluronic acid, and the inner hydrophobic layer is formed by the hydrophobic drug maytansine, so that the maximum tolerated dose of an anti-cancer drug can be improved to a great extent. Furthermore, the hyaluronic acid derived maytansine prodrug has a positive target capability without extra modifying target molecules, has a high enrichment ratio on a tumor part, has high cytotoxicity to tumor cells and canwell inhibit tumor growth in the in-vivo treatment process for a tumor-bearing naked mouse.

The invention discloses a preparing method of a kind of rose collagen peptide powder belonging to the field of food resource development and processing technology, which is characterized in that the coniferous fruit powder and the rose oil are added on the basis of the collagen peptide. The method has following advantages: combination of ingredients of the rose collagen peptide powder is reasonable; synergistic interaction of all ingredients is significant without toxic, side effects and additives; the whitening and anti-wrinkling effect, moisturizing and smoothing effect, anti-acne and dark spots effect, and anti-coarse effect are provided; drinking and carrying are convenient; the production process is simple and safe, operability is strong, the production cycle is short, thereby suitable for industrial production.

The present invention relates to a g-C3N4/ TiO2 coating with light-control and antibacterial functions and a preparation method thereof. The light-control antibacterial coating consists of TiO2 with athree-dimensional network structure and graphite-like phase carbon nitride (g-C3N4) deposited on the surface of the TiO2. The g-C3N4/ TiO2 coating with a three-dimensional network structure is combined with the surface of a titanium or titanium alloy substrate, rod-shaped nano-array sodium titanate is constructed on the surface of the substrate by a hydrothermal synthesis method, the nano-array sodium titanate is converted into titanium dioxide by a chemical vapor deposition technology, and the g-C3N4 is deposited at the same time. The preparation method comprises the following steps of: firstly, acquiring a rod-shaped nano-array sodium titanate biological coating on the surface of the substrate by adopting a hydrothermal synthesis method; and then converting the coating into titanium dioxide by using a high-temperature annealing technology, taking melamine as a raw material, and depositing g-C3N4 on the surface of the titanium dioxide through a chemical vapor deposition technology soas to finally obtain the g-C3N4/ TiO2 coating with the light-control and antibacterial functions.

The invention provides a myricetin lipid microcapsule taking lecithin as a carrier and a preparation method thereof. The myricetin lipid microcapsule is prepared by the following preparation method: weighing myricetin, rape lecithin or soybean lecithin, and stearic acid monoglyceride in proportion, adding adequate anhydrous ethanol for dissolving, evaporating the obtained solution under the conditions of vacuum degree of 0.080-0.085 MPa and temperature of 50-70 EDG C to recover ethanol to be dry, adding adequate tween 40 aqueous solution with the concentration of 0.3-0.4g/L into a residual material, fully dissolving under the ultrasonic condition, and standing for 5-10 minutes after completely dissolving to prepare the myricetin lipid microcapsule. The preparation method utilizes the lecithin of the vegetable oil materials for preparing the myricetin into the lipid microcapsule, with the particle size controlled to be 100nm-300nm; and the stability of the prepared plant flavonoid lipid microcapsule is obviously improved compared with flavonoid powder, especially the in vivo bioavailability is increased. Animal studies show that the absorption rate of the lipid microcapsule is increased by more than 2 times compared with original powder so as to more prominently give play to the biological activities of the plant flavonoid.

The invention discloses a low-toxicity 1,8-naphthalene dimethimide derivative as well as a preparation method and application thereof. The preparation method of the 1,8-naphthalene dimethimide derivative mainly comprises the following steps: dissolving amonafide and 3-fluorobenzoyl chloride into an organic solvent, performing reaction, and removing the solvent after reaction to obtain a target crude product. The experiment of the applicant indicates that the derivative has remarkable bioactivity, particularly has remarkable inhibition activity on a non-small cell lung cancer cell strain HCC-827 and other tumor cell strains, has smaller toxic and side effects on human normal cells, and is expected to be developed into a targeted therapy medicine. The structure of the 1,8-naphthalene dimethimide derivative is as shown in the formula (I): (the formula is as shown in the description).

The invention discloses methods for preparing collagen oleostock and moistening eyedrops. The method for preparing collagen oleostok comprises the following steps of: soaking raw materials with bromogeramine; then soaking with the mixed solution of peracetic acid and ethanol for sterilizing and decontaminating; and cleaning the sterilized and decontaminated raw materials and soaking with acetum to obtain the collagen oleostock. The method for preparing moistening eyedrops comprises the following steps of: performing enzymolysis and purification on the collagen oleostock to obtain collagen liquid; adjusting the concentration and osmotic pressure of the collagen liquid, filling under an aseptic condition and finally sealing to obtain disposable moistening collagen eyedrops. By using the method for preparing the collagen oleostock, various microbes and harmful substances in the raw materials can be killed effectively, and collagen cannot be inactivated. The moistening eyedrops prepared by using the method disclosed by the invention has a good treatment effect on dry eye syndrome, can restore cornea, is applicable to eye ball cleaning, lubricating and moistening, routine nursing care on dry eye syndrome and hemostasis and restoration of cornea injury.

The invention discloses a yogurt capsule and a preparation method thereof. The preparation method comprises the following steps: adding calcium lactate into yogurt, and uniformly stirring; then adding xanthan gum powder mixed with white sugar, and uniformly stirring to obtain a yogurt sample; dripping the yogurt sample into a sodium alga acid solution, and leaving to stand for a while to obtain a yogurt capsule. In every 100 g of yogurt, the dosage of calcium lactate is 2.7 g, the dosage of sodium alga acid is 1.1 g, the dosage of xanthan gum is 0.7 g, the dosage of white sugar is 6.3 g, and the mass ratio of xanthan gum to white sugar is 1:9. The preparation method has the following beneficial effects: the capsule spheroidizing technology is applied to wrap yogurt, and yogurt can be fixed and embedded into other food (such as jelly, ice cream, cake and cotton candy) in a nondestructive manner, so that the taste and the nutritional value of yogurt are not destroyed, the favorable bioactivity of yogurt is maintained, and a human being can enjoy the surprise brought by dual tastes.

The invention relates to a neurosteroids type novel GABA (gamma-aminobutyric acid) receptor modulator, pharmaceutically acceptable salt or ester, and application in treating related CNS diseases.The compound provided by the invention has favorable biological activity and an excellent PK property, and the novel compound has a large potential to be used as an CNS disease treatment drug for oraladministration.

The invention provides a culture medium for artificial propagation expanding of desert moss. The culture medium is characterized by comprising a Hogland culture medium and a plant growth regulator, wherein the plant growth regulator is 0.4-0.6 mg/L of 6-B-A or 0.4-0.6mg/L of 2,4-D. The invention also provides a method for artificial propagation of desert moss. By means of a propagation expanding matrix of desert moss crust and research on environmental conditions, the amplification mechanism of artificial moss crust materials having biological activity and self-propagation ability and the bestenvironmental conditions are determined, artificial moss crust materials having biological activity and self-propagation ability are cultured in a room, the short-term practice demand for sand fixation of human is met, and the purpose of treating land desertification is achieved.

The invention discloses a high-strength medical hydrogel based on humanized collagen and a preparation method thereof. The method comprises the following steps: taking sodium periodate as a selectiveoxidizing agent for performing open-loop oxidation on beta-cyclodextrin to prepare dialdehyde beta-cyclodextrin, using lysyloxidase for crosslinking the humanized collagen to prepare enzymatic self-crosslinking humanized collagen, adding the dialdehyde beta-cyclodextrin in the enzymatic self-crosslinking humanized collagen, and taking molecular self-assembly as a gel construction method to preparethe high-strength medical hydrogel based on humanized collagen. The high-strength medical hydrogel based on humanized collagen has good biological compatibility, biodegradability and biological safety, has good mechanical strength, and can be used as a haemostasis material, a tissue engineering scaffold, a high-grade dressing material, and a drug carrier material.

The invention provides a selenium-enriched biofertilizer and a preparation method of the selenium-enriched biofertilizer by complexing active bacteria and nano-selenium elements. According to the selenium-enriched biofertilizer, by means of chemical and physical mutagenesis and the like, the active bacteria and the nano-selenium elements are complexed through a reasonable coordination mode. A new strain contains the nano-selenium elements which have low toxicity and good biological activity, thereby the strain can be fully absorbed by human body, and the requirement of the human body for the selenium can be met.

The invention discloses an aryl triazole curcumenol derivative shown as a formula (I) and an application of the aryl triazole curcumenol derivative in the preparation of a medicine for treating humancolorectal cancer. According to the invention, a curcumenol 12-site double bond structure is modified; a plurality of curcumenol derivatives containing a triazole structure are obtained; an in-vitro cell experiment shows that the curcumenol derivative containing a triazole structure has good biological activity on a human colon cancer Sw620 cell strain, which can be used for preparing the curcumenol derivative containing a triazole structure and can be used to prepare a medicine for treating human colorectal cancer. The curcumenol derivative can prevent or/and treat human colorectal cancer, and has a huge application prospect in the field of medicine. The synthesis method of the curcumenol derivative containing a triazole structure is simple and convenient, mild in reaction conditions, andeasy to operate, moreover, the raw materials are easily available, the production cost is low, and the curcumenol derivative is suitable for industrial production and application.

The invention belongs to the technical field of biomedical materials, and particularly relates to a bone repair 3D printing material with low barium titanate content and a preparation method and application of the bone repair 3D printing material. The material is prepared by combining hydroxypropyl methyl cellulose, polyethyleneimine, barium titanate, tricalcium phosphate, a dispersing agent and the like according to a certain proportion, on the premise of ensuring the piezoelectric property, the content (smaller than or equal to 40%) of BT in a TCP/BT material system can be greatly reduced, and better biological activity, osteogenesis performance and degradation performance are obtained. Effective stimulation is formed on functions of proliferation, differentiation and the like of cells under the synergistic effect of the LIPUS, and the bone defect repair process is actively intervened. Besides, prepared slurry can also be combined with a 3D printing technology, so that personalized requirements of different patients in clinical use are met, and a personalized TCP/BT bone repair material is prepared.

The invention discloses a method for preparing a mass spectrometry organism complex sample. An organism sample is mixed with sodium alginate, and the obtained mixture is centrifuged and injected to obtain gel beads containing the sample to be detected, used as a mass spectrometry object, so the mass spectrometry sample processing process of the organism sample is simplified, and the use of chemical solvents is reduced, thereby the biological properties of the organism sample during analysis are guaranteed. Analysis of proteins, DNA and cells by using the method proves that the method can be used to perform simple processing in order to obtain effective organism matter information through the mass spectrometry.

The invention discloses a coumarin derivative and a preparation method thereof. The coumarin derivative has a structure represented by the formula (I) or (II). The coumarin derivative has higher bioactivity, better insecticidal effect and better solubility in water than coumarin. The preparation method of the coumarin derivative comprises the following steps: 7-hydroxyl-4-methyl coumarin is adopted as a raw material; under the effects of formaldehyde and organic primary amine or organic secondary amine, an amine methylation reaction is carried out, such that the needed coumarin derivative is obtained. The preparation method is simple, the raw materials are cheap and are easy to obtain, and thus the method is suitable for industrialized production. With the product and the method, a problem of limited coumarin extract source is solved. In the formula (I) or (II), R is n-alkyl with a carbon atom number of 1-4.

The invention discloses a graphene artificial cochlea electrode and a preparing method thereof. The electrode comprises a tip part, a bent part, contact electrodes, an electrode carrier and electrodewires, wherein the tip part is arranged at the foremost portion of the artificial cochlea electrode, the electrode carrier wraps the electrode wires and half wraps the contact electrodes connected with the electrode wires one to one, the bent part is an annular groove formed in the electrode carrier, each contact electrode comprises an inner side contact and an outer side contact, the inner side contact faces a worm shaft and the outer side contact backs to the worm shaft when the contact electrode is used and bent, and the electrode wires are wavy. The surface bioactivity of the contact electrodes is enhanced, the adhesion, proliferation and differentiation of cells are facilitated, meanwhile the formation and integration of peripheral nerves with the graphene artificial cochlea electrodeas the implant material are promoted, and the aim of improving the surface bioactivity and biocompatibility of the artificial cochlea contact electrodes is realized.

The invention discloses a method for preparing hemostatic sponge from oxidized bletilla striata modified collagen fibers. The preparation method comprises the following steps: oxidizing bletilla striata polysaccharide by adopting sodium periodate to prepare dialdehyde bletilla striata polysaccharide containing cross-linked active aldehyde groups, and chemically modifying collagenous fibers by taking the dialdehyde bletilla striata polysaccharide as a functional cross-linking agent. In the cross-linking process, the three-strand spiral structure of collagen is not damaged, and the porous three-dimensional network structure brings excellent filling power and water absorption performance. The blood coagulation activity of bletilla striata polysaccharide in the hemostatic sponge is well maintained, the platelet adhesiveness is remarkably improved, various blood coagulation indexes are superior to those of most of similar products, and the hemostatic performance is excellent. Meanwhile, the hemostatic sponge has excellent stability, the hemolysis rate meets the national safety requirement, the cytocompatibility is good, and the hemostatic sponge has the effects of promoting tissue growth, promoting wound healing and the like, and is an excellent novel hemostatic material.

The invention discloses a 1, 1 '-biphenyl-2, 6-diphenol compound and application thereof, and the 1, 1'-biphenyl-2, 6-diphenol compound is a new compound and has good biological activity. Through an in-vitro tumor cell activity experiment method, it is found that the compound has inhibitory activity on tumor cell proliferation and has the potential of developing anti-tumor drugs.

The invention belongs to the technical field of antibacterial medicines, and particularly relates to a ruthenium polypyridine complex with a triphenylphosphine structure, and a preparation method and application thereof. According to the ruthenium polypyridine complex with the triphenylphosphine structure, due to the fact that the ruthenium polypyridine complex contains metal ions and is charged, compared with traditional small organic molecules, the transmembrane effect and the retention effect are enhanced, and modification of different ligands can be carried out through the multi-coordination configuration of the metal complex, so the effect of better biological activity is achieved. Tests prove that the ruthenium polypyridine complex with the triphenylphosphine structure can effectively inhibit the growth of staphylococcus aureus and the formation of a biological membrane of the staphylococcus aureus when the content of the ruthenium polypyridine complex is 1.0-2.0 [mu]g/mL, and the ruthenium complex does not have the tendency of triggering the drug resistance of bacteria, and improves the sensitivity of the staphylococcus aureus to aminoglycoside antibiotics obviously.

The compounds of glass frame are: (mol%) CaO 24-45, SiO2 34-50, Na2O 0-25, P2O5 5-17, MgO 0-5 and CaF2 0-1. Volume percentage of porosity is 40-80, aperture is 50-600 um. Biological active glas powder produced by melting method is used as raw material, adding organic or high polymer pore-forming agent with different granularities in, cellular material biscuit is formed by dry pressing or gel injection molding after mixing, the biscuit is roasted on proper raising temp. to produce degradated cellular biological active glass frame that mechanics strength, prosity and hole size can be controlled. There is hydroxyl kietyoite generated on surface of said frame after the frame is steeped in human body analog humor, it has good biological activity and can be used as hard tissue defect repairing material and cell frame material for in vitro bone tissue cultivating.

The invention belongs to the field of plant protection, and relates to a pesticide composition and an application field. The invention relates to a combination of ricin insecticides and conventional insecticides, mainly used for the control of harmful arthropods in crops. The invention firstly discloses a method for extracting ricin-containing insecticidal active substance, and secondly, disclosesa ricin insecticidal substance and conventional insecticide composition and application. The ethanol solution is combined with the ultrasonic extraction to obtain the ricin insecticidal substance, and the obtained ricin insecticidal substance has a remarkable synergistic effect on a plurality of insecticides; the composition disclosed has the advantages of simple using method, convenient popularization and application, and good control effect on various target arthropods such as the cotton leaves, the spodoptera litura, the spodoptera litura, the diamondback moth, the worm, the whitefly, thealma and the leaves, and the like; the application amount of the pesticide can be reduced to a great extent, and the resistance development of the target arthropod to the pesticide can be delayed, meeting the requirements of current pesticide reduction and environmental protection.

The invention belongs to the field of extraction of a natural active product tremella polysaccharide in tremella fuciformis, and particularly relates to a method for extracting tremella polysaccharide from tremella fuciformis, which comprises the following steps: pretreating the raw material tremella fuciformis, crushing and sieving to obtain tremella fuciformis powder, selecting an efficient and suitable compound enzyme, reacting with the tremella fuciformis powder, and heating for enzyme deactivation treatment after enzymolysis is completed; then extracting at high temperature; performing sufficient stirring in the extraction process to ensure sufficient dissolution of polysaccharide; and after the extraction is completed, centrifugally separating the extracting solution, collecting the supernate, namely the polysaccharide component obtained by extraction, and determining the polysaccharide content by adopting a sulfuric acid-phenol method. The extraction rate of the tremella polysaccharide is greatly improved by combining compound enzyme catalytic conversion extraction with a high-temperature extraction technology; the purity of the extracted tremella polysaccharide is higher than that of tremella polysaccharide obtained through a traditional method, and biological activity is good; and the formula of the obtained specific compound enzyme is optimized, the extraction and preparation cost of the tremella polysaccharide is reduced, the method is suitable for industrial production, and the tremella polysaccharide can be more widely applied to the fields of cosmetics, food, medicines and the like.

The invention discloses a celecoxib derivative with a structure shown as a formula (I) or a pharmaceutically acceptable salt thereof and a preparation method and application of the derivative. The disclosed compound can be used as a substitute of celecoxib and can exist as a hydrate or a solvent, and the problems are solved that existing celecoxib medicaments are insoluble in water, poor in dissolution performance and low in in-vivo absorption rate. The structure-function relationship of celecoxib is analyzed and studied, and it is confirmed that compared with esterification of nitrogen-containing derivatives, sulfur-containing derivatives, oxy-ethers and alcoholic hydroxyl functional groups and other structural transformation, the disclosed celecoxib sulfonamide derivative has better medicinal bioactivity.