The invention discloses a double-anchoring
enzyme response polypeptide as well as a preparation method and application thereof, and the preparation method comprises the following steps: introducing a
gelatinase response sequence GPLGV between an
antibacterial peptide HHC36 and a
tissue repair promoting
peptide, and carrying out double-end azido modification to obtain the double-anchoring
enzyme response polypeptide. The
peptide for promoting
tissue repair can be
cell adhesion
peptide, osteogenic peptide, neurogenic peptide, angiogenic peptide and other functional polypeptides. The polypeptide is double-anchored on the surface of the
titanium sheet through double-end
azide modification. The activity of the polypeptide is closely related to the degree of freedom of the polypeptide, two ends of the polypeptide are fixed on the surface by
azide groups in a non-infection environment,
cell membranes cannot be destroyed through model antibacterial mechanisms such as annular holes, and at the moment, osteogenic activity is expressed. In a bacterial infection environment,
gelatinase secreted by
bacteria can enable antibacterial polypeptide to be unilaterally released to obtain
chain segment freedom, and the
antibacterial property is improved, so that antibacterial / osteogenesis functional switching of bacterial
gelatinase responsiveness is realized, and a functionalized
titanium-based
implant surface is constructed.