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High-strength medical hydrogel based on humanized collagen and preparation method thereof

A human collagen, high-strength technology, applied in the field of high-strength medical hydrogel and its preparation, can solve the problems of immune rejection of virus hidden dangers, incompatibility of biodegradation performance, and restrictions on medical applications, etc., to achieve the promotion of molecular autonomy Assembly behavior, improved mechanical strength and biodegradability, good water solubility

Inactive Publication Date: 2018-06-29
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In practical applications, it is found that collagen-based hydrogels still have inherent defects such as poor gel strength and unsuitable biodegradation properties, which greatly limit their medical applications. Therefore, it is urgent to develop a class of high-strength, controllable Degraded Collagen Hydrogel
[0003] In summary, in order to solve the hidden dangers of viruses and immune rejection that may exist in animal-derived collagen, the present invention uses human-derived collagen as raw material to ensure the biological safety of the material from the root; at the same time, in order to solve the problem of collagen Hydrogels have defects such as poor gel strength and unsuitable biodegradation performance. Dialdehyde β-cyclodextrin and lysyl oxidase are used as biological cross-linking agents, and molecular self-assembly is used as a gel construction method , a high-strength medical hydrogel based on human collagen was prepared. The hydrogel material has excellent properties such as good biocompatibility, biodegradability and biosafety, and has good Mechanical strength, can be used as hemostatic materials, tissue engineering scaffolds, advanced dressings, drug carrier materials, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Preparation of dialdehyde β-cyclodextrin: Take 1 weight part of β-cyclodextrin and add it to 50 volumes of phosphate buffer solution with a pH of 7.4, stir at room temperature until the solution is completely clear, and prepare β-cyclodextrin aqueous solution; then 0.5 parts by weight of sodium periodate are dissolved in 100 volumes of phosphate buffer solution with a pH of 7.4, stirred at room temperature until fully dissolved, and prepared into sodium periodate solution; then sodium periodate solution is slowly added to β-cyclodextrin aqueous solution, stirred and reacted in the dark at 4°C for 96 hours, after the reaction was completed, 20 volumes of polyethylene glycol was added to terminate the reaction, stirred at room temperature for 30 minutes, and dialyzed in deionized water using a dialysis bag with a cutoff of 300Da 5 days, and finally freeze-dried to prepare dialdehyde β-cyclodextrin;

[0019] (2) Preparation of enzymatically self-crosslinked human colla...

Embodiment 2

[0022] (1) Preparation of dialdehyde β-cyclodextrin: Take 1 weight part of β-cyclodextrin and add it to 100 volumes of phosphate buffer solution with a pH of 7.4, stir at room temperature until the solution is completely clear, and prepare β-cyclodextrin aqueous solution; then 1.0 parts by weight of sodium periodate is also dissolved in 80 volumes of phosphate buffer solution with a pH of 7.4, stirred at room temperature until completely dissolved, and prepared into a sodium periodate solution; then the sodium periodate solution is slowly added to β-cyclodextrin aqueous solution, stirred and reacted in the dark at 6°C for 72 hours, after the reaction was completed, 10 volumes of polyethylene glycol was added to terminate the reaction, stirred at room temperature for 30 minutes, and dialyzed in deionized water using a dialysis bag with a cutoff of 400Da 4 days, and finally freeze-dried to prepare dialdehyde β-cyclodextrin;

[0023] (2) Preparation of enzymatically self-crosslin...

Embodiment 3

[0026] (1) Preparation of dialdehyde β-cyclodextrin: Take 1 weight part of β-cyclodextrin and add it to 200 volumes of phosphate buffer solution with a pH of 7.4, stir at room temperature until the solution is completely clear, and prepare β-cyclodextrin Aqueous solution; then 1.5 parts by weight of sodium periodate are dissolved in 50 volumes of phosphate buffer solution with a pH of 7.4, stirred at room temperature until fully dissolved, and prepared into sodium periodate solution; then sodium periodate solution is slowly added to β-cyclodextrin aqueous solution, stirred and reacted in the dark at 10°C for 48 hours, after the reaction was completed, 4 volumes of polyethylene glycol was added to terminate the reaction, stirred at room temperature for 30 minutes, and dialyzed in deionized water using a dialysis bag with a cutoff of 500Da 3 days, and finally freeze-dried to prepare dialdehyde β-cyclodextrin;

[0027] (2) Preparation of enzymatically self-crosslinked human colla...

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Abstract

The invention discloses a high-strength medical hydrogel based on humanized collagen and a preparation method thereof. The method comprises the following steps: taking sodium periodate as a selectiveoxidizing agent for performing open-loop oxidation on beta-cyclodextrin to prepare dialdehyde beta-cyclodextrin, using lysyloxidase for crosslinking the humanized collagen to prepare enzymatic self-crosslinking humanized collagen, adding the dialdehyde beta-cyclodextrin in the enzymatic self-crosslinking humanized collagen, and taking molecular self-assembly as a gel construction method to preparethe high-strength medical hydrogel based on humanized collagen. The high-strength medical hydrogel based on humanized collagen has good biological compatibility, biodegradability and biological safety, has good mechanical strength, and can be used as a haemostasis material, a tissue engineering scaffold, a high-grade dressing material, and a drug carrier material.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a high-strength medical hydrogel based on human collagen and a preparation method thereof. Background technique [0002] Hydrogel is a kind of polymer material with a three-dimensional spatial network structure composed of hydrophilic polymers or colloidal particles bonded by physical bonds or chemical bonds and a large amount of water. Hydrogel swells but does not dissolve in aqueous solution, making its basic structure and skeleton more stable, and its interior has a dense three-dimensional network void structure, which has good permeability to water vapor and gas, and can support cell growth, growth, and value-added More importantly, hydrogel provides a moist environment for wound healing, which conforms to the theory of wet wound healing, so it is widely used in wound dressings, bioadhesives, tissue engineering scaffold materials, and drug delivery carriers. Mater...

Claims

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Application Information

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IPC IPC(8): A61L24/00A61L24/08A61L24/10A61L26/00A61L27/26A61L27/52A61L27/58C08B37/16
CPCA61L24/0031A61L24/0042A61L24/043A61L26/0052A61L26/008A61L26/009A61L27/26A61L27/52A61L27/58A61L2400/04C08B37/0012C08L5/16C08L89/00
Inventor 刘新华罗晓民郑驰王学川赵金礼
Owner SHAANXI UNIV OF SCI & TECH
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