4results about How to "Ensure biosecurity" patented technology

The utility model relates to a kind of adipose tissue preparation devices, belong to adipose tissue preparation technical field, including outer tube, outer tube upper end sealing connection upper end cover, outer tube lower end sealing connection lower end cover, upper end cover, lower end cover and outer tube are jointly surrounded to form cavity;Outer tube center is set through center shaft assembly;Turbine blade is rotationally set on center shaft assembly, and turbine blade is located in cavity;The utility model only processes adipose tissue by mechanical shearing, avoids the damage of chemical substance to adipose tissue biological activity, guarantees the biological safety of transplant tissue;Rely on manually pushing and pulling center shaft assembly, drive turbine blade to rotate under the resistance of component in cavity, without power or other power source, simple structure, small and exquisite, convenient to carry and operate;Shear stress generated by turbine blade rotation is even controllable, can stably and quickly break adipose tissue, and the broken efficiency is high, and particle size uniformity is good, meet the requirement of clinical transplantation injection.

This invention discloses a traceable hydrogel for cartilage repair and its applications. The hydrogel, composed of chitosan, sericin, and collagen, forms a three-dimensional biomimetic network, within which a magnetic material is uniformly dispersed. The magnetic material is selected from nano-Fe3O4 or γ-Fe2O3. Addressing the shortcomings of existing hydrogels, such as insufficient mechanical strength, inability to perform non-invasive tracking, easy aggregation of magnetic nanoparticles, and safety risks associated with chemical cross-linking, the traceable hydrogel prepared by this invention can improve the compressive modulus of the hydrogel, promote the deposition of cartilage-specific matrix, and simultaneously retain the complete magnetic signal activity while avoiding the toxicity of chemical cross-linking. The hydrogel can rapidly gel at physiological body temperature, making it suitable for minimally invasive clinical injection, providing a new solution for cartilage defect repair that combines functionality and safety.

This invention discloses a semi-quantitative RT-LAMP method and kit for viral nucleic acid detection based on dispensed lyophilized microspheres and a multi-channel microfluidic chip. The method includes: designing two independent LAMP primer sets (P1 and P2) for each of the SARS-CoV-2 Omicron strain N gene, Zika virus NS5 gene, H1N1 influenza virus M1 gene, and H3N2 influenza virus NP gene; preparing two independent types of lyophilized microspheres by independently mixing RT-LAMP premix containing Bst DNA polymerase and other components with LAMP primer mixture and a lyophilization protectant; and using an N-channel microfluidic chip to perform serial dilutions and parallel RT-LAMP amplification of the same sample, determining the concentration of viral nucleic acid in the sample by the positive / negative inflection points of each channel. This invention is simple to operate, requires no equipment such as a real-time PCR instrument, and is suitable for rapid virus detection in various scenarios.

The application provides a high-nitrogen nickel-free austenitic stainless steel bracket and a preparation method thereof, and belongs to the field of austenitic stainless steel. The method comprises the following steps: casting an initial molten steel into a consumable electrode, placing the consumable electrode in an electroslag remelting furnace, remelting and refining by using a mixed slag system of base slag and composite premelted nitride slag, and performing stepwise pressurization during the remelting process to obtain a steel ingot; homogenizing the steel ingot, and then performing multi-directional forging; performing high-temperature solid solution treatment on the forged blank, and then performing quenching; performing cold deformation processing on the plate, and then performing secondary solid solution treatment and quenching; machining the semi-finished product into the shape of a bracket, and then performing surface electrolytic polishing and passivation treatment to obtain the high-nitrogen nickel-free austenitic stainless steel bracket. Through the whole-process collaborative control from the material metallurgical source to the final forming, the harsh requirements of the high-nitrogen nickel-free austenitic stainless steel bracket on biocompatibility, mechanical properties and corrosion resistance as a long-term implanted instrument in the oral cavity are systematically met.