219results about How to "Ensure biosecurity" patented technology

The present invention relates to the field of feed formula design, specifically to a novel green broiler chickens complete feed and technique of preparing the same. A safe, pollution-free novel green broiler chickens complete feed of the invention is composed of the following components by weight parts: 55 -60 parts of corn, 10 -20 parts of dehulled bean pulp, 2 -3 parts of corn protein powder, 2 -3 parts of corn alcohol dreg, 2 -5 parts of cottonseed meal, 2 -8 parts of Chinese herbal compound preparation, 0.3-0.4 parts of multi-vitamin, 0.2 parts of composite trace elements. Thebroiler chickens complete feed develops a novel pollution-free non-drug residue green broiler chickens complete feed by adding Chinese herbal compound preparation. The broiler chickens fed by the feed greatly enhances immune function, greatly enhances the immunity against the disease and substantially improves the survival rate.

The present invention provides a microbial sample processing device, comprising: a machine body provided with a machine chamber and a sealed movable door; and a sealed housing, a stretchable airbag, a sample tray, a mechanical arm, a bar code scanner, a liquefier, culture dishes, a gas-temperature sensor, and a temperature control device which are arranged in the machine chamber, wherein the sample tray is provided with a plurality of sample seats, the sample seats are used for placing the culture dishes, the liquefier is communicated with the culture dishes, each culture dish is provided with culture plates and a streak-inoculation pen used for streak inoculating the sample in the culture dishes from the liquefier on the culture plate. The microbial sample processing device is reasonable in structure, fast and safe, reliable and efficient, can complete the whole process of mixing and liquefying, streak inoculation, dilution and separation, and incubation of microbial community automatically in a one-stop manner, and can replace manual labor and ensure biological safety.

The invention discloses a positive control template for PCR detection on a drug resistance gene, a preparation method and a kit thereof, wherein the drug resistance gene is NDM-1, and the sequence of the NDM-1 gene is the sequence as shown in SEQ ID NO: 1. The method for preparing the positive control template comprises the following steps: a) carrying out point mutation on the NDM-1 gene sequence so as to obtain a mutant gene, wherein the sequence of the mutant gene comprises the sequence as shown in SEQ ID NO: 3; b) synthesizing the mutant gene and then cloning the mutant gene into plasmids; and c) linearizing the plasmids so as to obtain the positive template. By setting the positive template, the NDM-1 detection can be carried out quickly and accurately; and through carrying out the point mutation on the NDM-1 gene, that the positive template can be ensured not to be transcribed and translated correctly and only can be used as a sequence for positive control, thereby guaranteeing biosafety.

The invention provides a preparation method of a cartilage regeneration scaffold material. The preparation method is characterized in that fresh skin at back, face or buttock of a dead animal is taken as a raw material and is subjected to treatment processes such as deep and low-temperature treatment, depilation, inactivation of virus, decellularization, structure remodeling, crosslinkage, freeze-drying, re-modification and sterilization, thus obtaining a finished product. The cartilage regeneration scaffold material prepared by the method disclosed by the invention can be used for promoting adhesion and growth of cartilage cells and promoting healing of articular cartilage injuries. The product belongs to a natural extracellular matrix ingredient which is beneficial to adsorption, proliferation and differentiation of the cells, also has a certain mechanical strength, can exist in the body for a long time in the form of a tissue filler, also has good tissue affinity and compatibility, and can be used for inducing cartilage cells to grow therein so as to rebuild the cartilage, promote the adhesion and growth of the cartilage cells, promote the healing of the articular cartilage injuries and restore articular defects, so the cartilage regeneration scaffold material is a novel regeneration scaffold material for effectively restoring articular cartilage defects.

The invention belongs to the technical field of biomedicine, and provides a bioartificial liver system. The system comprises a plasma separation / retransformation circulation part and a biological liver circulation part. The two parts are connected with each other through a circulation pipeline. The biological liver circulation part comprises a constant-temperature box with a heat preservation effect, a double-cavity blood storage bag, an oxygenation column, a plasma circulating pump, a dissolved oxygen monitoring and temperature instrument and a flow guide type micro capsule suspended fluidized bed bioreactor, wherein the double-cavity blood storage bag, the oxygenation column, the plasma circulating pump, the dissolved oxygen monitoring and temperature instrument and the flow guide type micro capsule suspended fluidized bed bioreactor are sequentially connected to form a circulating system. By means of a turbine flow guide type inlet, flow distribution is carried out on a part of high-speed fluid of the inlet by the bioreactor, lateral shear force borne by micro capsules in a fluid force field is effectively reduced, damage to the micro capsules is reduced, and contact efficiency of cells cultured in the bioreactor and perfusate is improved. The bioreactor is used for bioartificial liver supportive treatment system in order to obtain more ideal effects on the aspects of treating serious hepatitis, hepatic insufficiency and other diseases.

The invention provides hydrogel which comprises the following raw materials in parts by weight: 1-50 parts of chitosan, 0.1-20 parts of a cross-linking agent and 10-100 parts of water, wherein the cross-linking agent comprises the following raw materials in parts by weight: 1-5 parts of a raw material A and 0.1-5 parts of a raw material B; after the raw material A is combined with the raw material B, a raw material C is connected with the final end of the mixture; the raw material A is polyethylene glycol; the raw material B is a degradable polymer; the raw material B is selected from one or more of polyglycollide, polylactide, polyglycollide lactide and polycaprolactone; and the raw material C is selected from one or more of glyoxylic acid, uronic acid and aldehyde benzoic acid. The invention further provides a preparation method of the hydrogel. The hydrogel has the beneficial effects that a reaction product of polyethylene glycol and the degradable polymer is enabled to react with glyoxylic acid, uronic acid and aldehyde benzoic acid, and the obtained cross-linking agent and chitosan form the hydrogel automatically in a solution.

The invention discloses a water-soluble chitosan antibacterial derivative and a preparation method thereof. The water-soluble chitosan antibacterial derivative comprises a chitosan framework and a bifunctional group for modifying amino on the chitosan framework, wherein the bifunctional group comprises guanidino and carbonyl methoxy polyethylene glycol. The side chain of chitosan is modified withguanidino and short-chain polyethylene glycol, and the chitosan achieves good antibacterial property and higher biological safety. According to the preparation method, firstly, the chitosan reacts with the carbonyl methoxy polyethylene glycol, water solubility of the chitosan is sufficiently improved, and biological safety is greatly improved; secondly, the chitosan reacts with thiourea trioxide,participation of the amino in subsequent reaction is effectively avoided if the guanidino is grafted in the first step, rich positive charge of the guanidino is effectively retained, and the positivecharge density of the water-soluble chitosan antibacterial derivative is ensured, so that the antibacterial effect of the water-soluble chitosan antibacterial derivative is better.

The invention provides an establishing method of a recoverable immortalized hepatic cell line. The establishing method comprises the following steps: separating a hepatic progenitor cell from liver in a mouse which is 12.5-14.5 days old in an embryonic period; guiding a retrovirus containing genes SV40T and HSV-TK into the hepatic progenitor cell; screening a monoclonal cell strain having a hepatic progenitor cell marker and having a function of differing to a mature hepatic cell; then guiding a retrovirus containing CD gene into the cell strain so as to obtain recoverable immortalized hepatic cell carrying double suicide genes. The cell strain can multiply in vitro to obtain the phenotype and functions of a normal hepatic cell, the safety of the immortalized cell becomes adjustable through controllable modes such as locus recombination and drug screening; when the cell is used in a human body, the biological safety of the immortalized cell is ensured to the greatest extent and the dangerousness of the immortalized cell is reduced; therefore, a reliable, safe and ideal hepatic cell material is provided for bioartificial liver technology.

The invention discloses an expression process of a coat protein gene of prunus necrotic ring spot virus (PNRSV), an antiserum and a kit. The expression method comprises the following steps of: extracting a ribose nucleic acid (RNA) of the PNRSV in an affected material; adopting a transcription-polymerase chain reaction (RT-PCR) amplification process to clone the coat protein gene of the PNRSV; connecting the obtained coat protein gene to a pGEM-T vector, obtaining a pGEM-T-PNRSV recombinant vector, and using sequence analysis to determine the sequence accuracy of the coat protein gene of the PNRSV; building an eukaryotic expression vector of the coat protein gene of the PNRSV; transforming a pichia pastoris GS115 strain, and inducing a pichia pastoris expression coat protein; and using Western-blot to detect the size accuracy of an expression proteine. The invention aims to provide the process for using the pichia pastoris to express the PNRSV coat protein gene, the antiserum, and a PNRSV ELISA detection kit.

The invention relates to an automatic cell culture box. A refrigeration storage device, a CO2 conservation device, a culture medium box or a midway recycling bin, a central incubator, an activation box and a microscope camera structure are respectively connected with a center control system; the refrigeration storage device is arranged on one side in the box body; the CO2 conservation device is arranged on the other side in the box body; the refrigeration storage device and the CO2 conservation device are arranged at an interval by a clapboard in the box body. Compared with the traditional manual culture box, the automatic cell culture box provided by the invention has the advantages that the efficiency is improved by 30 percent, cell culture can be automatically controlled, completely closed culture is realized, an opening step does not exist any more, and the biological safety of cells is thoroughly guaranteed; uniform cell culture standard is realized, cell culture for more people can be simultaneously realized, the efficiency is improved, the labor intensity is reduced, the using amount of consumed materials is reduced, and the culture cost is reduced; the culture box can be used in an ordinary environment, and GMP (Good Manufacturing Practice) building and equipment purchasing costs are greatly reduced.

The invention belongs to the technical field of biomedicines, and particularly relates to a double-drug loaded thixotropic hydrogel for tumor local treatment and a preparation method thereof. The thixotropic hydrogel can be used for treating tumors by local injection including but not limited to intratumor injection, peritumoral injection and the like), and comprises the following components in percentage by mass: 1.2-18 percent of silk fibroin and 0.2-8 percent of hydroxy propyl cellulose, wherein the mass ratio of silk fibroin to hydroxy propyl cellulose is 1.5-9; and the anti-cancer medicament comprises 60-1900 mu g/mL curcumin and 10-3200 mu g/mL of doxorubicin hydrochloride. The drug loaded hydrogel has thixotropic syringeability and long-time drug sustained-release effect.

The invention discloses a degradable self-locking cervical vertebra fusion cage and a preparation method thereof. The degradable self-locking cervical vertebra fusion cage is made by the high-strength composite materials made of polylactic acid and beta-tricalcium phosphate, and the biosecurity is high. The application of a self-locking insert can provide stronger initial mechanical stability after the implantation of the fusion cage and promote lumbar interbody fusion, thereby being beneficial to the repair of bone defects, and the self-locking insert can be filled into the bone defect part in the orthopaedic wounding, tumor, infection, and congenital malformation operations.

The invention relates to a clean water treatment combined system and a combined technology of water supply plants. The clean water treatment combined system comprises a high-efficient settling pond, an ozone biological active carbon unit and an immersed type ultrafiltration membrane pond which are connected successively. The ozone biological active carbon unit comprises a post ozone contact reactor and an upward flow ozone biological active carbon filter. Water flow direction of the upward flow ozone biological active carbon filter is upward, and active carbon is always in a fluidized state, so that in a process of back wash, water dedicated for back wash is not needed, and excellent wash effect can be achieved only by means of gas back wash. According to the clean water treatment combined system and the combined technology, high-efficient precipitation technology, upward flow ozone biological active carbon technology and immersed type ultrafiltration membrane treatment technology are combined, so that it is effectively ensured that various water quality indexes of discharged water are capable of meeting with or are superior to national Domestic Drinking Water Hygienic Standard (GB 5749).

The invention relates to the field of titanium and titanium alloy, in particular to an anodic oxidation coloring method free of introducing of elements inconsistent with titanium and titanium alloy base materials. The method comprises the following steps that 1, titanium and titanium alloy are ultrasonically cleaned in a Micro-90 cleaning solution; 2, the cleaned titanium and titanium alloy are put in an acid pickling solution to be treated; 3, the titanium and titanium alloy are washed thoroughly with purified water; 4, the cleaned titanium and titanium alloy are clamped on a specific hanging tool, and the hanging tool is put in an anodic oxidation coloring solution to be oxidized; 5, the titanium and titanium alloy subjected to anodic oxidation coloring is washed thoroughly with purified water; and 6, the thoroughly washed titanium and titanium alloy are blow-dried and then put in a clean transfer box to be transferred. According to the anodic oxidation coloring method, the safety of implantation medical instruments is guaranteed to the largest extent, meanwhile, titanium and titanium alloy with various surface states are subjected to anodic oxidation treatment through an improved acid pickling technology, and color consistency is guaranteed.

The invention discloses a building water supply pipeline circulation system and method for secondary water supply quality guarantee and belongs to the field of drinking water treatment. The system comprises a building water supply inlet pipe, a lifting or water storage device, a flow measurement or detection device, a backflow controller, a building water supply vertical pipe, a building indoor pipeline and faucet, a backflow pipe, an ultraviolet/titanium dioxide sterilizer, a backflow electromagnetic valve and a check valve. In the normal water consumption process, municipal water passes through the building water supply inlet pipe, the lifting or water storage device, the flow measurement or detection device and the building water supply vertical pipe to reach the building indoor pipeline or faucet. When the hydraulic retention time of tap water for secondary water supply reaches 2 h or above, water in the building water supply vertical pipe passes through the backflow pipe and the ultraviolet/titanium dioxide sterilizer to flow into the building water supply indoor pipe or the water storage device again, is mixed with fresh tap water and then is used by users again through a secondary water supply system, and therefore the biological safety and chemical safety of the tap water in the secondary water supply system are ensured.

A method of preparing fucoidan having an efficiently customized molecular weight and applications of the fucoidan are disclosed. The method includes subjecting kelp fucoidan that is adopted as a raw material to microwave dissolution and ultrasonic degradation to obtain a fucoidan degraded solution; grading the fucoidan degraded solution by utilizing high-flow-speed agarose gel chromatography; collecting a solution of low-molecular-weight fucoidan having the target molecular weight; subjecting the solution of fucoidan having other molecular weights to ultrasonic degradation again, performing grading by utilizing the high-flow-speed agarose gel chromatography, and collecting a solution of low-molecular-weight fucoidan having the target molecular weight; combining all target fucoidan solutions; filtering the obtained target fucoidan solution by utilizing a nanofiltration membrane; subjecting a filtration product to desalting and concentration; diluting the fucoidan concentrate again; then subjecting the diluted fucoidan concentrate to nanofiltration, concentration and desalting again; precipitating the fucoidan with an ethanol solution, and washing and drying the fucoidan to obtain a fucoidan product having the low molecular weight. The method keeps active components of the fucoidan to the utmost. The purity of a target product that is a fucoidan product having high purity and a uniform low molecular weight is as high as 95% or above.

The invention belongs to the field of biological materials, and relates to an antibacterial polylactic acid master batch, which is mainly composed of carbonization layer modified copper oxide and cuprous oxide nanoparticles and polylactic acid. According to the antibacterial polylactic acid master batch provided by the invention, the antibacterial performance of polylactic acid is greatly improved, the production process is simple, the raw materials are easy to obtain, and the cost is low; and the master batch has good biocompatibility, can be applied to antimicrobial products, and can be applied to the fields of food packaging, medical instruments, building material decoration, aquaculture and the like.