17results about How to "Ensure biosecurity" patented technology

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The invention discloses an enzyme preparation for degrading allergens of pet food and application of the enzyme preparation, the enzyme preparation is a pH-responsive acrylic resin-pectin composite double-response shell embedded enzyme-probiotic composite system, a core layer is heat-resistant neutral protease, and a middle layer is a compound of lactobacillus reuteri and bacillus subtilis. In the processing stage, the protease degrades macromolecular allergens, the composite shell in the gastrointestinal environment firstly triggers outer acrylic resin to be dissolved through weak alkalinity of the intestinal tract, then inner pectin is degraded by pancreatin, and probiotics are accurately released to metabolize residual sensitizing peptides. The enzyme preparation realizes full-chain desensitization, is high in survival rate of probiotics, is suitable for various pet foods, and is stable and reliable in desensitization effect.

The utility model relates to a kind of adipose tissue preparation devices, belong to adipose tissue preparation technical field, including outer tube, outer tube upper end sealing connection upper end cover, outer tube lower end sealing connection lower end cover, upper end cover, lower end cover and outer tube are jointly surrounded to form cavity;Outer tube center is set through center shaft assembly;Turbine blade is rotationally set on center shaft assembly, and turbine blade is located in cavity;The utility model only processes adipose tissue by mechanical shearing, avoids the damage of chemical substance to adipose tissue biological activity, guarantees the biological safety of transplant tissue;Rely on manually pushing and pulling center shaft assembly, drive turbine blade to rotate under the resistance of component in cavity, without power or other power source, simple structure, small and exquisite, convenient to carry and operate;Shear stress generated by turbine blade rotation is even controllable, can stably and quickly break adipose tissue, and the broken efficiency is high, and particle size uniformity is good, meet the requirement of clinical transplantation injection.

The utility model discloses a portable isothermal amplification nucleic acid detection bag based on phase change heat release, which relates to the field of isothermal amplification nucleic acid detection, and comprises a phase change material mounting bag 101, and a heat conduction main reaction cabin, an enzyme-free water mounting bag and a flow measurement chromatography test strip mounting cabin which are arranged on the phase change material mounting bag 101, a first weak sealing strip is arranged on the enzyme-free water installation bag, an outlet of the heat conduction main reaction cabin is communicated with an inlet of the infusion apparatus, an outlet of the infusion apparatus is communicated with a liquid inlet of the flow measurement chromatography test strip installation cabin, and a second weak sealing strip is arranged on the infusion apparatus. The problems that a current isothermal amplification nucleic acid detection device is complex in structure and high in cost are solved, and instrument-free reaction and result visualization functions aiming at portable isothermal amplification reaction can be achieved.

The application belongs to the field of agricultural pest prevention and control and the field of pesticides, and provides a method for preventing and controlling spider mites by using bifenthrin-ethomec and plant essential oil, which comprises the following steps: (1) applying a plant essential oil solution or suspension to crops in need of prevention and control; and (2) applying a bifenthrin-ethomec suspension concentrate to the crops in need of prevention and control 12-36 hours after the application of the plant essential oil solution or suspension. According to the method, a low concentration of plant essential oil is applied in advance before the application of bifenthrin-ethomec, so that the dilution multiple of bifenthrin-ethomec can be greatly increased while the prevention and control effect is maintained.

The application discloses a kind of biocompatible materials and preparation method for bionic porous intervertebral fusion cage, belong to intervertebral fusion cage material technical field.A kind of biocompatible materials for bionic porous intervertebral fusion cage, by weight parts includes the following components: polyether ether ketone 65-80 parts, modified functional filler 15-25 parts, hydroxyapatite 8-12 parts, flow modifier 0.5-1 part;The modified functional filler is graphene oxide coated modified barium titanate functional filler.By introducing surface conductive modified nano piezoelectric ceramic particles, realize the effective conversion and transmission of mechanical stimulation to bioelectric signal, this microstructure design makes the insulating polyether ether ketone matrix possess the electric activity of simulating natural bone stress-electric signal response mechanism, so as to be favorable to activate osteoblast activity and promote bone integration of implant interface.

The invention belongs to the technical field of biology, and relates to a construction method and application of a mouse exogenous gene expression model based on an adenovirus vector. The exogenous gene is delivered through the adenovirus vector, the exogenous gene is subjected to site-specific integration to the specific site of the mouse target cell by using the double recombinase, the efficiency is high, the integration site and copy number are controllable, the safety is good, various function acquired variations can be efficiently modeled, and the method can be applied to establishment of a tumor model and research of precision medical treatment.

The present application relates to the technical field of medical packaging materials, and provides a wet non-woven fabric-based medical sterilization packaging composite material and a preparation method thereof, which comprises a wet non-woven fabric layer subjected to densification and reinforcement treatment, a heat-sealing film layer, and an adhesive layer arranged between the two layers, wherein the adhesive layer is distributed in a non-continuous pattern to locally composite the wet non-woven fabric layer and the heat-sealing film layer. Through the synergistic design of densification reinforcement and local composite in a non-continuous pattern, the present application reduces the shedding rate while retaining the air permeation channels in the un-composite area, solves the contradiction between high density and high air permeability, and effectively disperses stress through discrete adhesive structures, thereby realizing the unity of low shedding, high air permeability and stable peeling strength.

This invention discloses a traceable hydrogel for cartilage repair and its applications. The hydrogel, composed of chitosan, sericin, and collagen, forms a three-dimensional biomimetic network, within which a magnetic material is uniformly dispersed. The magnetic material is selected from nano-Fe3O4 or γ-Fe2O3. Addressing the shortcomings of existing hydrogels, such as insufficient mechanical strength, inability to perform non-invasive tracking, easy aggregation of magnetic nanoparticles, and safety risks associated with chemical cross-linking, the traceable hydrogel prepared by this invention can improve the compressive modulus of the hydrogel, promote the deposition of cartilage-specific matrix, and simultaneously retain the complete magnetic signal activity while avoiding the toxicity of chemical cross-linking. The hydrogel can rapidly gel at physiological body temperature, making it suitable for minimally invasive clinical injection, providing a new solution for cartilage defect repair that combines functionality and safety.

The invention discloses an integrated patch for wound pH detection and nitric oxide gas treatment and a preparation method of the integrated patch, relates to a biomedical engineering device, and is used for pH monitoring and nitric oxide (NO) treatment at a wound. According to the system, the pH value of a wound is monitored in real time by adopting a colorimetric method, and a micro light-emitting diode (LED) is used as a switch to control the release of nitric oxide, so that slow-release treatment of low-concentration nitric oxide can be realized, and sterilization treatment can be performed under a high-concentration condition. The patch specifically comprises a flexible circuit and a PVA film, and wireless gas therapy is realized through light by fixing an NO donor in the PVA film. According to the invention, ultrasonic energy can be collected in a living body and is converted into an electric signal based on a piezoelectric effect, so that wireless energy supply is realized. According to the invention, slow release and rapid release of NO can be realized, and different requirements in medical scenes can be realized.

The utility model provides a bacterial liquid spraying device for manufacturing a mouse skin bacterial infection model, which comprises a fixed cylinder and two support rods, a through groove is arranged at the top of the fixed cylinder, a mounting groove is arranged at the front end of the through groove at the top of the fixed cylinder, a fixing mechanism is arranged in the fixed cylinder, sliding grooves are arranged at the top of the fixed cylinder on both sides of the through groove, and the sliding grooves are communicated with the through groove. Mounting plates are fixedly connected to the two ends of the top of the fixed cylinder, a motor is fixedly mounted on one side of the mounting plate at one end, a moving mechanism is arranged between the two mounting plates, and limiting mechanisms are arranged on the portions, on the two sides of the moving mechanism, between the two mounting plates. And then a moving block is driven to move, so that the moving block drives an adjusting plate below to move from the tail of the nude mouse to the head of the nude mouse, and uniform spraying is performed on the skin of the nude mouse through a through groove by utilizing a bacterial liquid spray head, so that the accuracy and repeatability of a research result are better, and the biological safety is better guaranteed.

This invention discloses a vehicle cleaning device for livestock farms. The device includes a ramp, a cleaning canopy installed on the ramp, a water guide frame installed on the ramp, water guide side barriers on both sides of the ramp, a groove in the center of the ramp, an even number of movable guide rails installed in the groove, a cleaning device installed on the even number of movable guide rails, and a cleaning drive installed at the top of the ramp. The cleaning canopy contains a canopy body, with lifting seats fixed to the outer walls on both sides. This invention, by creating a groove in the center of the ramp, allows vehicles to complete exterior cleaning with the assistance of the cleaning canopy and then stop. Subsequently, the cleaning drive activates, causing the movable guide rails to assist in the displacement of the cleaning device's platform. Simultaneously, fan-shaped sprayers clean the hidden parts of the vehicle's underside, while movable spray components on both sides repeatedly wash the inside of the vehicle and the wastewater generated during cleaning. Ultimately, this method is not limited by the terrain of the ramp and effectively ensures biosecurity in the livestock area.

The invention discloses preparation and application of a hyperbranched polymer with high gene transfection capacity, and belongs to the technical field of chemical synthesis and modification. The preparation method of the hyperbranched polymer with high gene transfection capacity comprises the following steps: adding an acrylate compound and organic amine into an organic solvent, dissolving, carrying out addition reaction, cooling, adding an end-capping reagent, and continuously reacting to obtain the hyperbranched polymer. The hyperbranched polymer prepared by the invention can solve the problem that the transfection efficiency is low after the existing precursor is polymerized, the transfection capacity of nucleic acid drugs in cells is remarkably improved while the biological safety is ensured, and the hyperbranched polymer is expected to replace viral vectors to be used as mainstream nucleic acid drug delivery vectors in the market.

The application provides a drug-loaded nanoparticle Cor@PDA-PEG@CD11b and a preparation method and application thereof, the preparation method comprises the following steps: S1, mixing cordycepin solution and PDA aqueous solution, so that the cordycepin and the PDA form a complex Cor@PDA through π-π stacking effect; S2, the obtained complex Cor@PDA is modified by PEG to prepare a complex Cor@PDA@PEG; S3, the obtained Cor@PDA@PEG is dispersed in a buffer solution, and an anti-CD11b antibody is added; after reacting for 24h, centrifugation, washing, collecting the precipitate, and drying, the Cor@PDA-PEG@CD11b is obtained. The drug-loaded nanoparticle Cor@PDA-PEG@CD11b prepared by the application provides a new idea and direction for the treatment of sepsis-related lung injury; through the characteristics of the PDA nanoparticles, the cordycepin is released in a controlled manner, and the problem that the cordycepin is rapidly oxidized and inactivated after entering the body is avoided, the bioavailability is improved, the specific antibodies on the surface of immune cells and the physiological characteristics are utilized, the immune cells are quickly and chemotactically recruited to the inflammatory injury site in the early stage of acute inflammation, the drug is quickly and accurately targeted, and the sepsis lung injury is reduced.

This invention discloses a semi-quantitative RT-LAMP method and kit for viral nucleic acid detection based on dispensed lyophilized microspheres and a multi-channel microfluidic chip. The method includes: designing two independent LAMP primer sets (P1 and P2) for each of the SARS-CoV-2 Omicron strain N gene, Zika virus NS5 gene, H1N1 influenza virus M1 gene, and H3N2 influenza virus NP gene; preparing two independent types of lyophilized microspheres by independently mixing RT-LAMP premix containing Bst DNA polymerase and other components with LAMP primer mixture and a lyophilization protectant; and using an N-channel microfluidic chip to perform serial dilutions and parallel RT-LAMP amplification of the same sample, determining the concentration of viral nucleic acid in the sample by the positive / negative inflection points of each channel. This invention is simple to operate, requires no equipment such as a real-time PCR instrument, and is suitable for rapid virus detection in various scenarios.

The application discloses a method for preparing special protein control products by using blood product intermediate product components and the special protein control products. The method provided in the application uses discarded intermediate product components in blood products as raw materials for preparing special proteins, which not only develops new raw materials with stable sources and controllable quality for preparing special proteins, but also opens up a new idea for recycling the intermediate products of blood products. The obtained products are guaranteed in biological safety, good in stability and high in cost performance.

The application provides a high-nitrogen nickel-free austenitic stainless steel bracket and a preparation method thereof, and belongs to the field of austenitic stainless steel. The method comprises the following steps: casting an initial molten steel into a consumable electrode, placing the consumable electrode in an electroslag remelting furnace, remelting and refining by using a mixed slag system of base slag and composite premelted nitride slag, and performing stepwise pressurization during the remelting process to obtain a steel ingot; homogenizing the steel ingot, and then performing multi-directional forging; performing high-temperature solid solution treatment on the forged blank, and then performing quenching; performing cold deformation processing on the plate, and then performing secondary solid solution treatment and quenching; machining the semi-finished product into the shape of a bracket, and then performing surface electrolytic polishing and passivation treatment to obtain the high-nitrogen nickel-free austenitic stainless steel bracket. Through the whole-process collaborative control from the material metallurgical source to the final forming, the harsh requirements of the high-nitrogen nickel-free austenitic stainless steel bracket on biocompatibility, mechanical properties and corrosion resistance as a long-term implanted instrument in the oral cavity are systematically met.