422 results about "SILK SERICIN" patented technology

There is provided a novel wound dressing material which has biocompatibility and infection controllability as essential properties required for such a material, especially excellent flexibility and water absorption properties, thereby accelerating smooth regeneration of a skin defect without stripping off the regenerating skin while removing the material from the skin. A healing agent is added to the wound dressing material which comprises an amorphous film of a crystallinity below 10% and contains fibroin and sericin as a main component.

A method for preparing a porous-three-dimensional scaffold good for tissue engineering is described. Sericin forms a three-dimensional scaffold with PVA after freeze-drying having glycerin as a plasticizer and genipin as natural crosslinking agent to help making a strong and stable matrix. Adding glycerin into scaffold gives good uniformity and porosity. Smaller pore sizes and better uniformity are obtained as the concentration of genipin in the scaffold increases. Glycerin retains a high moisture content to allow the presence of water molecule in the matrix structure. Adding genipin results in a higher degree of crosslinking within the scaffold. Crosslinking using genipin is most beneficial in preparing scaffold possesses the best biological and physical properties for wound healing. The present invention describes method for preparing crosslinked matrix whose composition can be appropriately tuned to obtain matrix with desirable characteristics for biological applications.

The invention discloses natural silk facial mask base cloth which is made of a 100% natural silk filament sheet. The silk filament sheet is subjected to deoiling and softening treatment and then subjected to hot rolling by a roller so that sericin left on the surfaces of silk filaments can be fixed in a bonding mode, and then the natural silk facial mask is formed. The natural silk facial mask base material is of a filament structure and is good in transparency, water absorbency and water-retaining performance and soft and is closely attached to the skin without fiber tows; moreover, sericin protein left on the surface of silk has various beautifying functions of whitening, absorbing ultraviolet light and the like, and the natural silk facial mask base cloth can be used in cooperation with beauty fluid and can also be used independently, is an ideal material for making a beauty make and has good market prospects.

The invention discloses liquid shampoo. The liquid shampoo is prepared from, by mass, 50-60 parts of water, 0.5-1.0 part of a phase-A hair conditioner, 0.1-0.2 part of citric acid, 14-18 parts of a phase-B surface active agent, 0.1-0.2 part of a chelating agent, 0.1-1.0 part of a softener, 1-5 parts of a phase-B hair conditioner, 5-15 parts of a phase-C surface active agent, 0.1-0.5 part of an anti-dandruff agent, 2-5 parts of a phase-D surface active agent, 2-5 parts of a phase-E hair conditioner, 3-8 parts of a skin conditioner, 1-4 parts of a wetting agent, 0.5-1 part of a pearling agent, 0.1-0.5 part of an emulsion stabilizer, 0.2-0.4 part of essence, 0.2-0.4 part of a corrosion remover and 0.8-2 parts of sodium chloride. The skin conditioner is a compound of hydrolyzed sericin, olive oil PEG-7 esters, panthenol and a plant extract and has the effect of reducing pore plugging.

The invention relates to enhancer Hr3 adopting the nucleotide sequence shown in the SEQIDNO: 1. The enhancer Hr3 can be used for preparing and recombining heterologous protein by bombyx mori. The invention further relates to a recombination carrier containing the enhancer Hr3, and the recombination carrier is prepared in such a manner that the enhancer Hr3 is connected with a Nco1 restriction site at the upstream of a promoter of a carrier containing no enhancer through the Nco1 restriction site of the enhancer Hr3. In the invention, functional elements such as the first grade enhancer, activating transcription factor IE1, untranslated region sequences (5' UTR and 3' UTR) and the like which are expressed by intensifier are identified in the cellular level, and an efficient and stable sericin I expression system is built by integrating optimum elements on the basis, so that efficient expression recombination heterologous protein can be made of middle silk gland of transgenic bombyx mori.

The invention discloses a sericin post-finishing method for cotton fabrics, which comprises the following steps: (a) configuring settling impregnation liquid: dissolving 3-5% of dissolvable sericin powder counted by mass in hot water, and then adding 1-3% of chitosan quaternary ammonium salt, fully stirring and dissolving, and adding 4-6% of cross-linking agent, and (b) completely steeping the cotton fabrics in the settling impregnation liquid at a solution bath ratio of 1:(30-50), steeping for at least 20 minutes, twice steeping and twice rolling, keeping mangle expression more than 80%, anddrying, thereby obtaining the cotton fabrics. The invention develops the post-finishing method special for the cotton fabrics. The cotton fabrics prepared according to the post-finishing method have better washing fastness and anti-bacteria rate; after the cotton fabrics are washed for five times, a dissolving loss rate is about 0.21% and the anti-bacteria rate is 93.8%; the practical use requirement is fully met; and an extraordinary technical effect is obtained.

The invention relates to the technical field of biomedicines, and in particular relates to a preparation and identification method of a domestic silkworm silk fibroin solution. The preparation method of the domestic silkworm silk fibroin solution sequentially comprises the following steps: (1) degumming, namely degumming domestic silkworm silks to remove silk sericin on the outer layer to obtain degummed silks; (2) dissolving the silks, namely dissolving the degummed silks by using a ternary solution containing calcium chloride to obtain a fibroin solution; (3) performing renaturation, namely sequentially performing dialysis treatment on the fibroin solution according to a concentration gradient from high to low by using a protein denaturant solution, and then performing water dialysis treatment to obtain a silk fibroin solution; and (4) storing, namely storing the silk fibroin solution. The invention also provides an identification method of the domestic silkworm silk fibroin solution. The method can be used for identifying the quality of the silk fibroin solution. The preparation method of the domestic silkworm silk fibroin solution disclosed by the invention is relatively high in economical performance, ensures that the molecular weight of the domestic silkworm silk fibroin solution is close to that of natural fibroin molecules, and is beneficial to the preparation of subsequent silk fibroin based biomaterials.

The invention relates to a silkworm fibroin heavy-chain gene mutation method, which specifically comprises the step of: acting mRNA (Messenger Ribonucleic Acid) of a coded zinc-finger nuclease sequence on loci 1325-1362 of a silkworm fibroin heavy-chain gene shown as SEQ ID NO:1 to form target positions for recognizing zinc-finger nuclease, thereby obtaining a series of silkworm fibroin heavy-chain mutated genes; and the mutated sequence can be applied to preparation of sericin and extrinsic proteins. Mutants provided by the invention have the following advantages that: (1) a posterior division of silkgland of a fibroin heavy-chain gene mutant provided by the invention serious degrades, a cocoon shell only contains the sericin synthesized and secreted by a middle division of silkgland, if the mutated strains are utilized to transgenically express the extrinsic proteins, the expressed amount and the purity of the extrinsic proteins can be greatly increased, and thus, a brand-new useful genetic material is provided for the development of a silkworm fibroin bioreactor; and (2) the cocoon shell of the mutated strain provided by the invention only contains the sericin, and thus, a new source is provided for the large-scale production of the sericin.

The invention discloses a preparation method of a cocoon sericin layer alcohol soluble substance, comprising the following steps: wetting clean cocoon shell, cocoon outer floss, waste silk or raw silk in water and carrying out degumming treatment for 1-5h under the conditions that the temperature is 110-140 DEG C and the pressure is 0.1-0.26Mpa to obtain silk degumming liquid; filtering and concentrating the silk degumming liquid to obtain concentrated sericin liquid and then adding an organic solvent for extraction; collecting supernatant and repeatedly extracting lower precipitate with the organic solvent; carrying out spray drying or vacuum freeze drying after concentrating combined supernatant to prepare powder, namely the cocoon sericin layer alcohol soluble substance; wherein, the volume ratio of the concentrated sericin liquid to the organic solvent is 1:1-9. The non-sericin component obtained by the invention has antioxidant activity, antityrosinase activity and alpha-glucosidase inhibition activity, and has wide application prospects in such aspects as cosmetics, anti-aging health care products, hypoglycemic drugs and the like.

The invention relates to a hydrolyzed sericin peptide preparation method, and belongs to the technical field of protein separation and purification. According to the present invention, tasar silkwormcocoon shell or waste mulberry silk is used as a raw material, the tasar silkworm cocoon shell is washed, a sericin solution is prepared, a hydrolyzed sericin peptide solution is prepared, an activated macroporous resin decolorization system is assembled, a hydrolyzed sericin peptide decolorization solution is prepared, an activated MCI GEL resin system is assembled, a hydrolyzed sericin peptide grading separation solution is prepared, a hydrolyzed sericin peptide grading separation concentration solution is prepared, a hydrolyzed sericin peptide grading separation ethanol removing concentration solution is prepared, and hydrolyzed sericin peptide freeze-drying powder is prepared so as to obtain the product; by using multi-enzyme combined hydrolysis, ultra-filtration, nano-filtration separation, macroporous resin decolorization, MCI GEL resin grading separation, low-temperature concentration, freeze drying, and other modern chemical industry technologies and equipment, such that the preparation method has characteristics of convenient operation, mild condition, high efficiency, energy saving, low production cost and the like; and the product prepared by using the preparation methodcan be widely used in the fields of nutritious daily chemicals, fiber modification, and the like.

The invention discloses silk protein powder and a preparation method thereof. The preparation method comprises the following steps of: (1) degumming silks; (2) performing enzymolysis on silk fibroins and sericin; (3) separating and purifying silk proteins; and (4) pre-concentrating and drying a silk protein liquid. The silk protein powder disclosed by the invention comprises sericin proteins and silk fibroin proteins, has the advantages of high purity, high biological activity, proper molecular weight, white color, zero foreign smell and the like and can be used for making biological and medical materials, health protection food and the like, and the aims of pure nature, high activity, high functionality, rich nutrition and the like are fulfilled.

The object is to provide a method for stabilization of a biological molecule and a composition, specifically a method for stabilization of an enzyme or a labeled antibody for use in a clinical diagnosis and a composition. Thus, disclosed is a method for stabilization of a biological molecule which is characterized by allowing (a) the biological molecule and (b) sericin and / or a hydrolysate or equivalence thereof to coexist with each other. Also disclosed is a composition having a biological molecule stabilized therein, which is characterized in that the components (a) and (b) coexist with each other in the composition. Further disclosed is a composition for stabilizing a biological molecule, which comprises sericin and / or a hydrolysate or equivalence thereof.

The invention discloses a high-quality mulberry silk manufacturing technology. The technology includes the specific steps of cocoon stripping, cocoon assorting, vacuum steaming, ultrasonic cooperative cocoon cooking, silk reeling, re-reeling, post-processing, testing, packaging, storage and the like. By means of the technology, cleanliness of silk is improved, and silk reeling quality is comprehensively improved, so that under the action of ultrasonic waves, the inside and the outside of a cocoon shell achieve the effects that sericin is uniformly swovlen, shell dropping on the inner layer is reduced, the reelability rate is improved, steaming efficiency is improved, work energy consumption is lowered, neatness and cleanliness of raw silk are improved, and fineness continuous dynamic non-contact detection of the raw silk is achieved. Quality of the raw silk is improved, raw silk drying efficiency is improved, and work energy consumption of equipment is lowered.

The invention provides a chitosan-sericin composite biological scaffold as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving chitosan by taking an acetic acid solution as a solvent to obtain a chitosan solution; preparing a sericin aqueous solution from the cocoon of bombyx mori of the fibroin-deleted mutant variety; uniformly mixing the chitosan solution and the sericin aqueous solution and pouring into a mould; adding a crosslinking agent for moulding to obtain a chitosan-sericin composite hydrogel; and freeze-drying the chitosan-sericin hydrogel to obtain the chitosan-sericin composite biological scaffold. The invention also provides a composite biological scaffold carrying a nerve growth factor NGF for peripheral nerve entrapment treatment. The composite biological scaffold provided by the invention has good biocompatibility and relatively high porosity and degradability, and the degradation product also has a neurotropic effect; the composite scaffold also has antibacterial and anti-adhesion functions and the like; and compared with the traditional way of relieving sciatic nerve compression simply by operation, the composite biological scaffold provided by the invention can effectively promote the repairing of peripheral nerve entrapment injury.

The invention provides a three-dimensional parallel collagenous fiber-silk bracket as well as a preparation method thereof. The bracket is formed by a woven silk base frame and a parallel collagenous fiber layer which coats on the upper and lower surfaces of the woven silk base frame, wherein the woven silk base frame adopts a mesh structure which is woven by silk and has a mesh size of 0.25-25 mm<2>; the pore diameter of the parallel collagenous fiber layer is 10-300 microns; the woven silk is sericin-removed woven silk; the bracket can be used as a biological bracket and applied to the repair of muscle tendons / ligaments. The three-dimensional parallel collagenous fiber-silk bracket provided by the invention is simple to prepare, good in biocompatibility and good in mechanical property, has a topological structure similar to muscle tendons / ligaments and can be used for promoting the surface grown stem cells to differentiate toward the muscle tendons / ligaments and promoting the regeneration of the muscle tendons / ligaments.

The invention relates to a preparation method for a carbon-film-coated zinc oxide hollow sphere. Pollen is taken as a template for preparing a ZnO hollow sphere, and sericin is used as a carbon source for performing carbon film coating; the condition does not exist before the application; the preparation method comprises the steps of fully mixing zinc salt with an ethanol solution of pollen, and adding alkali liquid to a water bath in a dropwise manner; performing aging, centrifuging and separating to obtain ZnO precipitate with a pollen template; performing high temperature sintering on dried white powder to obtain the ZnO hollow sphere; immersing the ZnO hollow sphere powder into a sericin solution, and performing centrifuging, separating and drying; and performing vacuum sintering on the dried powder to obtain the carbon-film-coated ZnO hollow sphere taking the sericin as the carbon source. The preparation method is low in cost, simple in process, low-carbon and environment-friendly; and the obtained carbon-film-coated ZnO hollow sphere can be used as a lithium ion battery negative electrode material, a photocatalytic material and the like.

The invention belongs to the technical field of nanomaterials and relates to a preparation method of silk fibroin-based nano MOF (metal-organic framework) materials. The MOFs in different nano shapes can be prepared through adjustment of the ratio of rare-earth ions to silk fibroin macromolecules. The preparation method of the silk fibroin-based nano MOF materials comprises steps as follows: silk fibers are degummed and then dissolved and dialyzed in a ternary solution prepared from calcium chloride, ethanol and water, a silk fibroin solution with certain concentration is prepared, under normal temperature or hydrothermal reaction conditions, the silk fibroin solution is induced by different amounts of the rare-earth ions or mixtures of the rare-earth ions and micromolecular polycarboxylic acid, and protein macromolecule-based MOFs adopting different nano structures are synthesized under the molecular self-assembly action. The preparation method is simple and easy to implement, raw materials are easy to obtain, and the silk fibroin-based MOF materials in various nano shapes can be prepared.

A method for preparing a sericin hydrogel, the method including: 1) weighing a cocoon of a fibroin-deficient mutant silkworm, Bombyx mori, extracting the cocoon by an aqueous solution of LiBr or LiCl, dialyzing an extracted solution to yield a sericin solution having a concentration of a non-degraded sericin of between 0.1 and 4 wt. %; and 2) concentrating the sericin solution to a concentration of between 1.5 and 10 wt. %, adding a crosslinking agent to the concentrated sericin solution at a ratio of between 2 and 500 μL of the crosslinking agent per each milliliter of the sericin solution, fully blending the crosslinking agent with the concentrated sericin solution, and keeping a resulting mixture at the temperature of between 4 and 45° C. for between 5 s and 36 hrs to yield a hydrogel.

The invention discloses a dyeing and finishing process of polyester and cotton blended fiber fabric. The process comprises pretreatment, presetting, modification treatment, dyeing, mordant removal, soaping, color fixing, after finishing, drying, sizing, calendaring and rolling. Snailase and alpha-amylase are adopted for pretreatment, modification treatment is adopted before dyeing, the polyester and cotton blended fabric is modified fundamentally, and the dye-uptake of the fabric is increased; in the dyeing procedure, fewer alkaline substances and inorganic salt are used, a dye leveler, substitution alkali, a migration inhibitor and a diffusant are added, the dye leveling performance is improved, natural dye is used, environmental pollution is avoided, and the environment-friendly requirement is met; self-cleaning treatment is added during aftertreatment, and the self-cleaning capacity, the crease-resistant performance, the wash durability and color fastness are improved; silk fibroin and sericin are adopted for afterfinish, and the anti-pilling performance and the antistatic performance of the fabric are provided; the whole process is environment-friendly and pollution-free.

The invention relates to sericin / oxidized graphene composite hdyrogel as well as a preparation method and application thereof. The preparation method comprises the steps: blending a methacrylic anhydride modified sericin solution and oxidized graphene, then adding a photoinitiator and performing crosslinking under ultraviolet irradiation to prepare sericin / oxidized graphene composite hydrogel withgood mechanical property and osteogenic induction performance. The prepared sericin / oxidized graphene composite hydrogel has the advantages of good biocompatibility and biodegradability, high mechanical strength and osteogenic induction and application to the aspects of medicine and cell carrying, tissue engineering repairing and the like.

The invention relates to a natural sericin encapsulated mesoporous silicon nano-carrier and a preparation method and application thereof to the field of tumor treatment. The preparation method comprises the steps of preparing pure sericin solids; preparing mesoporous silicon nano-particles; preparing aminated, formylated or dual-sulfurated mesoporous silicon nano-particles with regular pore passage; preparing drug-loaded aminated, formylated or dual-sulfurated mesoporous silicon nano-particles; preparing a pH / protease or reduction / protease dual-responsiveness sericin / mesoporous silicon composite multifunctional control system. The natural sericin encapsulated mesoporous silicon nano-carrier and the preparation method and the application thereof have the advantages that the operation is simple to perform, the raw materials are simple and convenient to obtain, the cost is low, the universal applicability is high, no special complex equipment is needed and the like. The nano composite system prepared by adopting the method fully utilizes the characteristics of the sericin on the outer layer and the mesoporous silicon nano-particles on the inner layer, has pH / protease or reduction / protease dual responsiveness, has multiple functions of inhibiting tumor cells, monitoring nano-particle positioning and drug release in real time and the like, is high is drug loading capacity, is good in biocompatibility and has a wide application prospect in the field of tumor diagnosis and treatment, especially in the aspect of solving multi-drug resistance of tumors.

A modified human acidic fibroblast growth factor gene is disclosed. The nucleotide sequence of the gene is shown as the number 25 locus to the number 450 locus of SEQ ID NO.1. The sequence is designed according to codon preferences of bombyx mori, and can be normally expressed and translated in the bombyx mori. A recombinant vector containing the modified human acidic fibroblast growth factor gene is also disclosed. The recombinant vector comprises an enhanser hr3 and a secreting type sericin 1 promoter Ser1, specifically expresses a modified human acidic fibroblast growth factor in a middle silk gland cell of the bombyx mori, and secretes a recombinant protein to a sericin layer of silk. The obtained silk has bioactivity, has a function of promoting cell proliferation when the silk is directly used, and is a good medical material.

The invention belongs to the technical field of textile engineering and provides a refining technology of silkworm oil rotten cocoons. The refining technology of the silkworm oil rotten cocoons is achieved through the following steps: primary refining cocoon boiling, biological decay, washing and dewatering, immersion cleaning, washing and dewatering, scouring and bleaching, washing and dewatering, secondary refining, washing and dewatering, softening, drying and cotton opening. By means of the refining technology, the biological decay enables silk fibers of the oil rotten cocoons to be fully dissociated and then mutually adhered together, accordingly short fibers are not prone to be omitted during later cocoon opening process, needle rollers are convenient to be hung, and use ratio of the silk fibers is improved. By adopting reasonable proportioning of various auxiliaries during different procedures, such as boiling refining, decaying, scouring and bleaching, damage to the silk fibers from chemical auxiliaries is reduced to the largest extent, oil removing effect is improved, sericin protective layers outside fibroins are reasonably protected, strength of the silk fibers is protected, comprehensive use ratio of oil rotten cocoons is improved, and the refining purposes including oil removing, rubber retaining, and fiber tensile force protection are achieved.

The invention relates to a sericin protein microneedle for drug delivery and a preparation method thereof, belonging to the technical field of biomedical engineering. The microneedle is prepared through the following steps: firstly, providing a sericin protein solution, dissolving the sericin protein solution in ultrapure water, carrying out magnetic stirring and dispersion, and mixing a drug required to be delivered in the sericin protein solution according to a doctor's advice, wherein the concentration of the sericin protein solution is 0.1-500 mg / mL; and then, preparing a sericin protein microneedle: filling the prepared sericin protein solution in a microneedle slot of a customized microneedle female mold, and drying and curing; and stripping the sericin protein microneedle from the microneedle female mold, so that the sericin protein microneedle is obtained. Compared with traditional microneedles, the sericin protein microneedle not only has excellent biocompatibility and antibacterial properties, but also has a series of prominent advantages of controllable slow release, high efficiency, low cost, easy preparation, flexibility, natural biodegradability, and the like, can be directly used in organisms, and has good performances under various environmental interferences, therefore, the microneedle has a good application and development prospect.

The invention discloses a preparation method of a green metal absorbing material with cellulose and sericin. The preparation method has the key points that the cellulose and the sericin protein of bamboo are utilized and the green metal absorbing material with high absorbing efficiency and full biodegradation is prepared by molecular design and chemical modification. The preparation method is simple, high-efficiency and no-pollution. The green metal absorbing material has the advantages that the cellulose and the sericin protein of the bamboo are introduced into raw materials for preparing the metal absorbing material, the range of the raw materials for preparing the metal absorbing material is expanded, an innovative idea for recycling the cellulose and the serich protein of the bamboo is provided, the breakthrough of the technical problem at the section of removing metal ions in industrial and domestic waste water is accelerated and great environmental and social benefits are achieved.

The invention relates to a composite protein peptide with skin care efficacy, a hydrogel thereof and a preparation method. The composite protein peptide comprises the following components by weight: 1.0-7.0 parts of silk fibroin peptide, 1.0-7.0 parts of casein peptide, and 0.1-2.0 parts of sericin. The composite protein peptide hydrogel comprises the following components by mass percentage: 0.5-20% of the composite protein peptide, 1.5-2.5% of an additive, 0.3-0.6% of a preservative, and the balance water, wherein the additive is one or more of xanthan gum, Carbomer, sodium alginate, and glycerin. The composite protein peptide and the hydrogel thereof provided by the invention have water supplementing, moisturizing, whitening, oxidation resisting and other skin care efficacy.

The invention relates to a gel material, in particular to an alginate complex gel stent material constructed based on homogeneous phase crosslinking and layer-by-layer self-assembly technology and a preparation method thereof, and belongs to the technical field of tissue engineering. Alginate gel is prepared with an HAP / GDL compound as an endogenous crosslinking agent, wherein the mole ratio of HAP to GDL is 1:10, and meanwhile, it is determined that the mole ratio of the Ca element in the HAP to -COOH in sodium alginate (SA) is 0.18-0.36; afterwards, chitosan (CS) with positive electricity and B-type gelatin (GT) (collagen or sericin) with negative electricity are alternately deposited on the surface of the alginate gel through the electrostatic effect; and finally, the GT (collagen or sericin) on the outermost layer is subjected to covalent crosslinking through an EDC / NHS mixture, and therefore an alginate complex gel stent with homogeneous phase crosslinking is prepared.

The invention relates to a preparation method of a sericin-polyvinyl alcohol blended antibacterial film loaded with nano-silver, and a product and application of the antibacterial film. The preparation method is characterized in that silkworm cocoons are treated at high temperature and high pressure to obtain a sericin solution; then, the sericin solution and a prepared polyvinyl alcohol solution are blended, and are poured into a container; through freezing-unfreezing circulation, sericin-polyvinyl alcohol blended gel is obtained; drying is performed; a sericin-polyvinyl alcohol blended film is obtained; finally, the prepared film is added into an AgNO3 solution; the sericin-polyvinyl alcohol blended antibacterial film loaded with the nano-silver is obtained by an ultraviolet radiation reduction method. The prepared sericin-polyvinyl alcohol blended antibacterial film loaded with the nano-silver has good mechanical performance, water absorption performance and antibacterial performance, and is hopeful to be applied to the field of biomedical materials.

The invention discloses a reticular tissue engineering scaffold, which is characterized in that: it is mainly woven from sericin removed silk, the mesh size is 0.5-20mm<2>, and the composite biomaterial in the meshes is any of collagen, a medical polyester polymer synthetic material, chitosan, hyaluronic acid, and chondroitin sulfate. The reticular structure of the scaffold provided in the invention has both good mechanical properties and enough interlinked cell tissue accommodation space. The employed materials are easy to obtain, and have excellent mechanical and biological properties. The scaffold also has sufficient pores and good mechanical properties, and is suitable for repair and tissue engineering of ligaments, tendons, abdominal walls, pelvic floors and other tensile tissues.

The invention relates to a sericin microsphere as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, extracting sericin from silkwormcocoon according to a high-temperature alkaline extraction method and preparing into a pure sericin solution; secondly, adding the sericin solution into corn oil, stirring, dispersing, and dropwise adding a cross-linking agent to obtain the sericin microsphere; thirdly, adding acetone into mixed emulsion and curing the sericin microsphere; fourthly, washing and drying to obtain a sericin microsphere solid; fifthly, co-incubating the sericin microsphere with medicines to obtain a drug-loaded sericin microsphere; sixthly, adding phenolic compounds and metal ions under neutral or slightly alkaline conditions to obtain the metal organic coordination polymer coated drug-loaded sericin microsphere. The sericin microsphere prepared by the invention has the characteristics of stable structure, uniform particle size, good dispersion in water, and capability of retaining fluorescence characteristics of sericin. The metal organic coordination polymer coated drug-loaded sericin microsphere has thecharacteristics of high drug loading capacity, pH sensitivity and the like.