96 results about "SILK SERICIN" patented technology

The invention discloses preparation and application of bacteriophage-loaded sericin microgel, and belongs to the technical field of food preservation. The sericin microgel capable of loading the bacteriophage is prepared from a sericin solution extracted by using 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) in combination with a mechanical shearing method and a high-temperature and high-pressure method. The sericin protein microgel loaded with bacteriophage, which is obtained by soaking the microgel into bacteriophage proliferation liquid after freeze-drying, can significantly improve the storage stability of the bacteriophage and can effectively prolong the shelf life of beef, and the preparation method is green, simple and safe, has a wide application prospect, and is suitable for industrial production. Meanwhile, a new field is opened up for efficient application of the bacteriophage.

The invention belongs to the technical field of antibacterial materials, and particularly relates to a sericin / tannic acid-silver antibacterial-anti-biofilm nanocomposite as well as a preparation method and application thereof. According to the method, the TA-Ag nano material is prepared by adopting a simple water bath method; and then, dispersing SS in a TA-Ag aqueous solution to realize hydrogen bond combination of SS and TA so as to obtain the SS / TA-Ag nanocomposite with a stable structure. In the preparation process, plant polyphenol tannic acid is taken as a carrier, silver ions and tannic acid are combined by adopting a water bath method, and the antibacterial property of tannic acid is effectively improved; and the sericin is further modified through hydrogen bond crosslinking, so that the obtained composite material not only has antibacterial and anti-biofilm dual functions, but also is simple in preparation process and mild in reaction condition, and conforms to green chemistry and environment-friendly concepts.

The invention belongs to the field of biomedical materials, and particularly relates to a coagulation-promoting embolism microsphere and a preparation method thereof. The sericin-based embolism microspheres are creatively prepared, and the sericin membrane layer on the surface of the microspheres can prevent the risk that a catheter is solidified during an operation due to thrombin burst release during the operation. On the other hand, sericin in the microspheres has excellent water absorption swelling property and lubricity, and is beneficial to embolism and prevention of reflux; moreover, sericin molecules contain a large amount of hydroxyl and carboxyl, so that the chelation of blood coagulation factor calcium ions is greatly improved. In addition, thrombin factors are loaded into the microspheres, calcium ions are chelated on the surfaces of the microspheres, the microspheres are endowed with rapid embolism capacity through the superimposed effect, blood flow blocking of target blood vessels can be effectively accelerated, and reflux is prevented. The prepared procoagulant embolism microspheres are suitable for being applied to treatment of vascular-rich tumors of the brain and can effectively prevent mistaken closure of many complex functional blood vessels of the brain due to reflux.

This invention provides an antimicrobial peptide spray, its preparation method, and its application in the prevention and treatment of peristomal skin infections. The spray comprises, by weight percentage, 0.05%-0.5% of a complex antimicrobial peptide, 0.1%-5% of a moisturizing and repairing film-forming matrix, 0.05%-0.2% of a buffer, 0.8%-1.2% of an isotonic regulator, 0.01%-0.5% of a stabilizer, and 0.005%-0.05% of a metal ion chelating agent, with the balance being water for injection. The complex antimicrobial peptide is composed of bovine lactoferrin antimicrobial peptide, sericin oligopeptide, and buckwheat-derived anti-inflammatory active peptide in a weight ratio of (3-5):(2-3):1. This spray possesses four core functions: broad-spectrum antibacterial activity, inhibition of bacterial biofilm, anti-inflammatory and soothing effects, and skin barrier repair. It is less likely to induce bacterial resistance, does not irritate damaged skin around the stoma, and can effectively prevent and treat peristomal skin infections.

The invention relates to a soft capsule, in particular to a long-acting DHA soft capsule and a preparation method thereof. The long-acting DHA soft capsule is prepared from the following raw materials in parts by weight: 10 to 11 parts of xanthan gum, 3 to 4 parts of locust bean gum, 0.5 to 1 part of sericin, 0.5 part of glycerol and 13.5 to 16 parts of deionized water. The preparation method comprises the following steps: stirring and heating to 75-80 DEG C until all the raw materials are dissolved in water or melted and solid particles visible to naked eyes do not exist, so that the raw materials are glue liquid; spreading the glue solution into a rubber sheet; pressing the capsule skin and the DHA grease composition into capsules through a soft capsule pelleting machine; after shaping, polishing and drying to obtain the long-acting DHA soft capsule. The capsule skin of the long-acting DHA soft capsule is of a single-layer structure, the structure is simple, the physicochemical property is determined, a traditional soft capsule pelleting machine can be used for pressing, an additional secondary coating process is not needed, and the production process is simplified; through cooperation of xanthan gum and locust bean gum, the gum skin keeps certain dimensional stability in a freezing environment.

The invention relates to the technical field of biological medicines, in particular to application of canthaxanthin extract and a feed additive containing the canthaxanthin extract in silkworm breeding, and finds that cocoon shell amount, whole cocoon amount and pupa weight of silkworms can be remarkably increased and egg laying amount of single moths can be increased by adding canthaxanthin extract to silkworms for the first time. Meanwhile, the silkworm variety with functional carotenoid binding protein can also be induced to produce orange red natural colored cocoons. The invention further provides a compound feed additive containing the canthaxanthin extract and the sericin-derived small peptide and / or gamma-aminobutyric acid. Experiments prove that the canthaxanthin extract and the sericin-derived small peptide have a synergistic effect in the aspect of increasing the cocoon shell amount. According to the technical scheme, on the premise that normal growth and development of silkworms are not affected, the multiple purposes of yield increasing, propagation promoting and color increasing are achieved at the same time, and the method has good application prospects and industrial value.

The utility model discloses a dehumidification structure and a dehumidification rotating wheel. The dehumidification structure comprises a moisture absorption medium layer, a sericin layer surrounding the peripheral side of the moisture absorption medium layer, a first connecting assembly and a second connecting assembly with elastic recovery capacity. The first connecting assembly comprises a plurality of fixed connecting columns capable of conducting heat, and the two ends of each fixed connecting column are connected with the moisture absorption medium layer and the sericin layer correspondingly; the second connecting assembly comprises a plurality of springback connecting columns; each springback connecting column penetrates through the moisture absorption medium layer in the thickness direction of the moisture absorption medium layer, and the two ends of each springback connecting column are connected with the corresponding sericin layers on the two sides respectively; according to the dehumidification structure, the moisture absorption performance can be improved by effectively utilizing the expansion characteristic of the moisture absorption medium layer, the expansion of the moisture absorption medium layer can be restrained to avoid excessive expansion, and the dehumidification structure can be used in the dehumidification rotating wheel.

The invention discloses a hydrogel composite scaffold as well as a preparation method and application thereof. The hydrogel composite scaffold comprises a hydrogel matrix and drug-loaded microspheres loaded on the hydrogel matrix, the hydrogel matrix is grafted with a photosensitive group; the drug-loaded microsphere comprises a microsphere matrix and a drug loaded on the microsphere matrix; the hydrogel matrix and the microsphere matrix respectively and independently comprise natural protein and derivatives thereof; the natural protein and the derivative thereof comprise at least one of silk fibroin, sericin, collagen or gelatin. According to the invention, the natural protein and the derivative thereof are grafted and modified by adopting a photosensitive group, so that the injectable property can be realized, and the formed hydrogel matrix has good biological activity, can promote adhesion and proliferation of cells, and is combined with the drug-loaded microspheres to realize controlled release of drugs and promote regeneration and repair of damaged tissues. The hydrogel composite scaffold can be widely applied to preparation of biomedical materials, in particular to preparation of cartilage repair materials.

The application discloses a processing technology and cocoon silk, and processing is performed according to the following steps: S1: swelling dispersion and anti-sticking treatment: placing silk containing sericin in a weak acid buffer prepared from an organic acid, the pH value being 1.0-4.0, and performing swelling treatment by heating, unsaturated fatty acid is added in the weak acid buffer in advance or is added in the initial stage of swelling in step S1, S2: post-treatment: taking out the swelled silk and drying, and performing drying to obtain dispersed single-fiber cocoon silk which does not adhere to each other. Through the two-step cooperation of 'pH-temperature dominant swelling' and 'oleic acid preventing secondary adhesion', the prepared cocoon silk retains 60%-90% of natural sericin and is in a fluffy and dispersed single-fiber state; the cocoon silk replaces the traditional degumming process, reduces pollution, has high spinnability, and the retained sericin has excellent biological activity and biocompatibility, so that the cocoon silk material has higher competitiveness and can become a raw material of biomedical textiles.

The embodiment of the present application provides a preparation method of a composite hydrogel, comprising the following steps: S1, preparing methacrylated gelatin (GelMA); S2, separating a degumming solution of silk to obtain an HMSS solution, wherein HMSS refers to sericin with a molecular weight greater than 30kDa; S3, preparing the HMSS / GelMA composite hydrogel: dissolving the prepared GelMA in deionized water, adding a photoinitiator LAP to obtain a GelMA gel system, then mixing the GelMA gel system with the HMSS solution to obtain a pre-polymer mixture, and irradiating the pre-polymer mixture under ultraviolet light to obtain the HMSS / GelMA composite hydrogel; the HMSS / GelMA composite hydrogel prepared in this way has high porosity and good strength performance, and meets the application in the direction of tissue repair; and the present application further provides the composite hydrogel prepared by using the preparation method.

The utility model discloses a silk sericin purification device, specifically relates to purification device technical field, including purification box, the purification box bottom wall fixed setting has main discharge pipe, two branch discharge pipes are connected to the main discharge pipe bottom, two branch discharge pipes outside wall all are fixedly set up with a connecting block, two connecting blocks bottom all rotatoryly connected with a rotating seat, rotating seat outside wall fixed setting has two connecting plates, two connecting plates are away from each other one end all are fixedly set up with a conversion seat, the filter core is arranged in the conversion seat, and the locking assembly is arranged between the connecting block and the rotating seat, through the setting of two branch discharge pipes, when the filter core replacement in a branch discharge pipe, the silk sericin of finishing purification is discharged through another branch discharge pipe, will not influence the main discharge pipe, branch discharge pipe silk sericin of finishing purification and discharge, improve the practicality of device.

The application belongs to the technical field of genetic engineering, and particularly relates to a gRNA targeting Bombyx mori sericin 1 gene, a method for preparing coarse silk and application. The target sequence of the gRNA is shown as SEQ ID NO:1. The method for preparing coarse silk comprises the following steps: 1) constructing a gRNA expression vector targeting the Bombyx mori BmSer1 gene, and preparing a gRNA transgenic Bombyx mori; 2) crossing the gRNA transgenic Bombyx mori and Cas9 transgenic Bombyx mori, and screening to obtain double transgenic individuals with green fluorescence in eyes and bodies; 3) screening to obtain a heterozygous mutant Bombyx mori strain with the BmSer1 gene knocked out through typing detection; and 4) obtaining coarse silk by feeding the transgenic Bombyx mori. The genetic editing technology is used to change the genetic traits of Bombyx mori, and the obtained silk has a larger diameter, a lower sericin rate, better water resistance and durability.

The present application relates to a kind of judging methods of degumming state of silk, belong to silk fibroin production technical field, the degumming is to cut dry cocoon and put into degumming system and carry out degumming, by obtaining the first degumming liquid data and first silk data in the first degumming stage, obtain the second degumming liquid data in the second degumming stage, derive degumming liquid data change value, obtain second silk data based on degumming change value and first silk data, further judge the degumming state of silk, can only be determined by the conductivity of degumming solution and ultraviolet absorption peak value on-line judge degumming process corresponding degumming point silk fibroin degumming rate and silk fibroin molecular weight, can be used to monitor degumming progress, to control process to accurately control the molecular weight of silk fibroin, and suitable for alkaline degumming system and enzyme degumming system, provide universal judging standard, in the regulation silk fibroin molecular weight, cocoon raw material selection degumming process, screening cocoon raw material has important research significance and application value.

The invention relates to a natural, sustainable, eco-friendly, cost-effective ultraviolet (UV) blocker and tyrosinase enzyme inhibitor cosmetic raw material, obtained from silkworm excrement, which is improved in quality, stabilized to prevent activity loss, free of side effects, stable and long-lasting, and to a preparation method thereof, for use in the cosmetics sector in particular, as well as in various other industrial fields. The inventive ultraviolet (UV) blocker and tyrosinase enzyme inhibitor cosmetic raw material is obtained by using sericin protein, fulvic acid, and optimized silkworm excrement extract.

The present application provides a sericin / guar gum / polyvinyl alcohol (PVA) composite gel and its application as a fever-reducing patch. For the first time, sericin, cationic guar gum and PVA are used as a composite matrix, and sodium periodate and copper sulfate are introduced simultaneously. Through the precise assignment and synergistic effect of functional units, the problems of microbial contamination and insufficient high-temperature stability are systematically solved. The composite gel prepared by the present application has the advantages of initial adhesion (instant fixation), environmental stability (resistant to heat 42℃ / resistant to cold 0℃), good sustained cooling effect and skin safe pH (4.5-7.0), and is very suitable for clinical application.

This invention provides a sericin microsphere based on colored cocoons, its preparation method, and its applications, relating to the fields of biomaterials and microfluidic processing technology. The preparation method includes the following steps: (1) dissolving sericin in formic acid or dimethyl sulfoxide, adding an organic solvent, and adding or not adding a polymer to obtain an organic phase containing sericin; (2) dissolving an oil-water interface stabilizer in deionized water to obtain an aqueous phase; (3) using the organic phase as the dispersed phase and the aqueous phase as the continuous phase, preparing oil-in-water microdroplets using a capillary glass tube microfluidic device; (4) solidifying the microdroplets to obtain sericin microspheres. The process for preparing sericin microspheres using this invention is simple, mild, and bio-friendly. The sericin microspheres have uniform particle size, good monodispersity, and abundant autofluorescence properties. The sericin microspheres of this invention can be used in fields such as bioimaging, tissue engineering, cell culture, adsorption separation, drug delivery, and biosensing.

The invention discloses a preparation method of ZIFs-based sericin protein composite aerogel capable of efficiently hydrating and converting CO2. The method comprises the following steps: firstly, carrying out carboxylation modification on sericin (SS), anchoring with Zn < 2 + >, and then adding 2-methylimidazole (2-MI) to prepare powdery ZIF-mono-Seri nano-enzyme with a lamellar structure; the ZIF-mono-SeriGel composite aerogel is compounded with sodium carboxymethyl cellulose (CMC), and finally the ZIF-mono-SeriGel composite aerogel is obtained. The carboxylated SS is preferentially coordinated with Zn < 2 + >, so that the ZIFs are promoted to be converted from a three-dimensional structure to a two-dimensional lamellar structure, high-dispersion growth of the ZIFs on an SS molecular chain is realized, and the catalytic activity of the ZIFs on a CO2 hydration reaction can be remarkably enhanced through an asymmetric coordination structure between interfaces of the carboxylated SS and Zn < 2 + >. In addition, the SS side chain is easy to form a hydrogen bond network, so that the proton transfer rate in the CO2 hydration reaction can be greatly accelerated, and the step is a speed limiting step of the reaction. The CMC is used as a reinforcing skeleton, so that the structure forming and immobilization of the nano-enzyme are realized, and the problems that a powder material is difficult to recycle and difficult to produce on a large scale in practical application and the like are effectively solved.

The invention discloses a deep eutectic solvent regulated and controlled sericin modified carbon nanotube-acrylamide composite conductive hydrogel as well as a preparation method and application thereof, and relates to the field of flexible electronic devices and biosensing. The preparation method comprises the following steps: firstly, mixing sericin and carbon nanotubes under a water phase condition, carrying out ultrasonic treatment, and constructing an SS-coated CNT composite solution through a non-covalent coating effect; then, a choline chloride-urea deep eutectic solvent is introduced, and a composite interface is reconstructed and stabilized through interaction of multiple hydrogen bonds; and finally, adding an acrylamide monomer, a cross-linking agent and an initiator, and carrying out thermal initiation polymerization to form the three-dimensional network conductive hydrogel. The deep eutectic solvent is used as an interface regulation factor to promote the formation of a continuous conductive network, and the piezoresistive sensitivity and the linear response interval of the hydrogel can be synergistically regulated by adjusting the dosage of the deep eutectic solvent; the process of firstly constructing SS (at) CNT in a water phase and then introducing the deep eutectic solvent can effectively inhibit carbon nanotube agglomeration and sericin chain collapse, and the structural stability, the electrical continuity and the cycle durability of a conductive network are remarkably improved.

The invention discloses a dual-module sericin-based composite microsphere and a preparation method and application thereof.The dual-module sericin-based composite microsphere comprises an inner core and a shell coated on the outer surface of the inner core, the inner core is a sericin template mineralized ZnSr-Se-HA nano-particle, the shell is a tea polyphenol-cerium EGCG-Ce metal phenolic aldehyde coating, and the outer surface of the inner core is coated with the ZnSr-Se-HA nano-particle. The sericin-based composite microspheres (SeHA-EC) with ZnSr-Se-HA nanoparticles as an inner core and an EGCG-Ce metal phenolic aldehyde coating as a shell are prepared through an electrostatic microfluidic technology. The composite microsphere takes natural sericin as a substrate, has good biocompatibility, and can avoid foreign matter reaction caused by traditional synthetic materials; the particle size of the composite microsphere is controllable, and the dissection requirement of the periodontal pocket can be met; according to the composite microsphere, a strontium / zinc multi-ion therapy is combined with metal phenolic aldehyde, so that multi-target intervention of oxidation resistance, immunoregulation and angiogenesis and osteogenesis is realized.

The present application relates to printing and dyeing process technical field, specifically to a kind of silk gauze alkali gel degumming method, comprising the following steps: S1. A small amount of food-grade sodium polyacrylate is added to sodium hydroxide solution at a constant speed, and an electric mixer is used to continuously stir thoroughly, so that sodium polyacrylate is stirred uniformly, and an alkali gel with a certain amount of alkali agent is prepared;S2. The fabric is weighed and fixed on the printing table version, and the prepared alkali gel is single-sidedly scraped with a printing screen to form a large square pattern. Then, the fabric with scraped gel is clamped between two layers of cotton cloth and placed in a hot press flatbed machine for degumming at a set temperature and time. After degumming, repeatedly wash with cold running water until the sizing is washed clean and the washing water is neutral. Then, dry in a 95℃ oven for 2 hours, weigh and store after overnight equilibration, and repeat the operation 3 times to complete degumming. The alkali gel can form a relatively stable alkali environment on the surface of the fabric, allowing the alkali agent to fully contact and react with sericin, thereby improving the degumming effect.

This invention discloses a wound dressing with photothermal antibacterial, hemocoagulant-promoting, and easily removable properties, its preparation method, and applications, belonging to the field of biomaterials technology. The method includes dissolving sericin in water, then sequentially adding hemoglobin, carbodiimide, and N-hydroxysuccinimide to induce a cross-linking reaction; obtaining a precursor for the composite protein dressing through a mechanochemical cross-linking strategy; pre-cooling the precursor and freeze-drying it to obtain a sericin-hemoglobin dressing; dialyzing the sericin-hemoglobin dressing in water for 2-3 days, and freeze-drying it again to obtain a protein dressing containing only hemoglobin and sericin. The antibacterial dressing prepared by this invention, upon contact with blood, promotes the formation of a dense fibrin membrane, promoting hemostasis while making it easy to remove from the wound site. This wound dressing is entirely composed of protein, exhibits good biocompatibility, and has great development potential.

The invention relates to the technical field of environmental protection, in particular to a treatment process of tussah soda boiling wastewater, which comprises the following steps: step 1, burning rice husks, putting the rice husks into an adsorption column after burning, pumping soda boiling wastewater to soak the adsorption column, flowing out of the adsorption column, and enriching in a primary treatment storage tank to obtain a primary recycled water body; measuring the pH and the protein content of the primary recycled water body in the storage tank; step 2, adding phenolphthalein polyarylether sulfone immobilized protease into the primary recycled water body in the step 1 for enzymolysis adsorption, and filtering to obtain polyarylether sulfone particles adsorbed with sericin protein enzymolysis peptide and secondary filtered wastewater; and step 3, adding sodium carbonate or sodium hydroxide into the secondary filtered wastewater in the step 2 to adjust the pH value, pumping the wastewater back to the cocoon cooking pool for secondary tussah alkali cooking degumming, and recovering a solid mixture of rice hull ash and coarse sericin.

This invention provides a method for producing processed silk yarn suitable for forming silk fabrics and knitted silk fabrics that can be used for general clothing. [Solution] The method for manufacturing silk processed yarn according to the present invention comprises a preparation step S1 of preparing two or more types of silk yarns with different degrees of shrinkage in response to salt shrinkage treatment; an entangled yarn generation step S2 of generating entangled yarns having entangled portions arranged at unequal intervals from the two or more types of silk yarns prepared; a salt shrinkage step S4 of applying salt shrinkage treatment to the generated entangled yarns; and a sericin fixing step S2 of fixing sericin contained in the two or more types of silk yarns before they become entangled yarns, or in the entangled yarns before they are subjected to salt shrinkage treatment.

The present application relates to the technical field of high polymer materials, and more particularly to a preparation process of a compatibilizer, an article and application thereof, and provides a preparation process of a compatibilizer, which comprises the following steps: 1) adding polyester material and solvent into a three-necked flask, heating, stirring, introducing nitrogen, adding a reaction monomer, adding an initiator after stirring, and obtaining a product after reaction; 2) after cooling the product, adding an extraction liquid while stirring, vacuum filtering, and obtaining the product after purification. The compatibilizer prepared by the process has high grafting rate, which is beneficial to improving the total grafting rate, and when applied to silk sericin composite material, the mechanical properties and thermal properties of the composite material are improved, the maximum degradation temperature can reach 402 DEG C, and the application prospect is wide.

The application discloses a preparation method of natural rubber composite material and application thereof, and mixes natural rubber with modified carbon nanotube dispersion liquid, stirs, blends, and then dries and dehydrates to obtain the natural rubber composite material. The application has simple equipment and simple process, the natural rubber composite material after silk fibroin modified carbon nanotube is manufactured has electromagnetic shielding performance, the mechanical property is obviously improved, the application of the natural rubber material in the electromagnetic shielding field is expanded, and the application has the prospect of large-scale preparation.

The invention relates to the technical field of wound dressings, in particular to a sericin composite hydrogel dressing, a preparation method and application. The preparation method of the sericin composite hydrogel dressing comprises the following steps: taking 0.05 to 0.25 percent of blue light initiator solution, sequentially adding 14 percent of acrylamide monomer and 0.07 percent of N, N '-methylene bisacrylamide into the blue light initiator solution, and uniformly mixing to obtain a first solution; adding 5%-15% of sericin protein into the first solution, and uniformly mixing to obtain a second solution; adding 0.5%-5% of tannic acid into the second solution, and uniformly mixing to form a PAM-Ser-TA hydrogel precursor solution; and placing the PAM-Ser-at-TA hydrogel precursor solution in a mold, and irradiating the PAM-Ser-at-TA hydrogel precursor solution with blue light for 30-60 seconds to obtain the sericin protein composite hydrogel dressing. The sericin composite hydrogel dressing provided by the invention is used for wound repair, has good moist environment keeping ability, oxidation resistance and biodegradability, and is suitable for acute and chronic wounds or infectious wounds.

The present application relates to a kind of dry eye drops, it includes by mass percentage: chondroitin sulfate 0.1%-0.5%, silk fibroin nanoparticles containing astaxanthin 0.06%-0.3%, sodium hyaluronate 0.1%-0.3%, vitamin E 0.05%-0.1%, sodium chloride 0.9-1.0%, poloxamer 0.05%-0.1%, EDTA 0.01%-0.1%, phosphate buffer and the balance of water;Wherein, the silk fibroin nanoparticles containing astaxanthin particle size is 50-300nm;Phosphate buffer is adjusted to 6.8-7.9 to the pH of eye drop.The eye drop of the present application can effectively relieve dry eye symptoms, provide long time wet and protection, while reducing eye inflammation and oxidative stress, significantly improve dry eye, and high use safety.

The application relates to the field of immunology, and provides an application of silk sericin and silk polypeptide, researches on extraction and purification methods of silk sericin and polypeptide, finds that the protein and polypeptide can promote maturation of dendritic cells, enhance expression of MHCII and CD86 on the cell surface, enhance antigen presentation capacity, and stimulate lymphocyte T cell proliferation in cooperation with the dendritic cells; researches on a preparation method of nanoemulsion vaccine prepared by taking the silk sericin and polypeptide as an adjuvant and antigens, and detection finds that the silk sericin and polypeptide can improve the immune activation efficiency of the vaccine, obviously enhance the antigen-specific acquired immune response level in the body, and effectively inhibit the virus infection of the body and the replication level of the virus, so that the purpose of enhancing the vaccine in preventing or treating diseases is achieved.

The invention discloses an SS / CTGF composite biological material based on carboxyl chitosan as well as a preparation method and application of the SS / CTGF composite biological material. A substance containing active sericin (SS) and CTGF at the same time is extracted from genetic engineering silkworm cocoons over-expressing human connective tissue growth factors (CTGF) by utilizing an alkali extraction-acid protection method. Furthermore, the active substance, carboxyl chitosan and beta-glycerophosphate are used as raw materials, and the CH-SS / CTGF composite hydrogel biological material with the core-shell structure is prepared through thermal induction gelation. The material has the characteristics that the water content is adjustable, the mechanical property is controllable, and active protein can be quickly released. Compared with pure sericin, the active SS / CTGF substance and the composite hydrogel thereof can more remarkably promote cell proliferation and migration, and show good biocompatibility. The biological material prepared by the invention has wide application prospects in the fields of wound healing, drug delivery and regenerative medicine.