165 results about "SILK SERICIN" patented technology

The invention relates to the technical field of plastic bags, in particular to an antibacterial degradable plastic bag and a preparation method thereof. The antibacterial degradable plastic bag is prepared from the following raw materials in parts by weight: 60-80 parts of polyhydroxyalkanoate, 30-40 parts of filler, 2-4 parts of an antioxidant and 0.6-1 part of an active agent. In the preparation process of the antibacterial degradable plastic bag, in a filler system, vesuvianite powder and eggshell powder are used as inorganic reinforcing phases, sericin and mulberry leaf powder are used as bioactive phases, flax and cotton provide a natural fiber structure, and lignin and konjac glucomannan are used as bio-based bonding materials; and the eggshell powder is compact in crystal structure after being subjected to high-temperature calcination treatment, and can be used as a stress transfer medium in a material system, so that the stress concentration when the plastic bag is stressed is reduced, and the strength performance of the plastic bag is improved.

The invention relates to the technical field of biological material preparation, and particularly discloses a method and a system for industrial continuous production of silk fibroin. In order to solve the problems of high energy consumption, heavy pollution, low efficiency, poor product stability and the like in the prior art, efficient removal of sericin is realized through a gradient degumming synergistic treatment technology (high-temperature and high-pressure alkali treatment and directional enzymolysis), and desalination and concentration are synchronously completed in combination with a membrane separation directional purification technology (a hollow fiber ultrafiltration system). The system integrates the gradient degumming module, the dissolution-filtration coupling module, the membrane separation module and the resource circulation subsystem, the technological parameters (degumming rate, viscosity and transmembrane pressure difference) are regulated and controlled in real time through the intelligent control center, the continuous production stability is ensured, the purity of the obtained silk fibroin solution is larger than or equal to 99%, and the standard of beauty makeup materials is met. The method and the system for industrial continuous production of silk fibroin have the advantages of high efficiency, low consumption and environmental protection, and are suitable for large-scale production.

The invention discloses a preparation method and application of an environment-friendly easily-degradable hydrogel coating, and relates to the technical field of hydrogel coatings. The coating is a coating comprising chitosan, acrylic acid, sericin, silk fibroin, silk peptide, tea polyphenol, a photoinitiator, a plasticizer and a leveling agent. The preparation method comprises the following steps: (1) preparing the coating containing the components; (2) coating the surface of food packaging paper with the coating liquid in a slit coating manner; and (3) performing ultraviolet light initiation to obtain the coating. The hydrogel coating has a synergistic effect with the sericin, the silk fibroin, the silk peptide and the tea polyphenol to form a compact and uniform network structure, so that the water-retaining property, the mechanical strength, the flexibility and the antibacterial property are enhanced; the preservative not only has the advantages of environmental protection and degradability, but also has the functions of moisturizing, antibiosis and preservation, and can significantly prolong the shelf life of food and improve the quality. The environment-friendly packaging paper is applied to food packaging paper, not only conforms to the green packaging trend, but also expands the use scenes of environment-friendly materials in the fields of fresh food, baking and the like.

The invention relates to the technical field of textile fabrics, in particular to a moisture-absorption breathable polyester and chinlon composite fiber and a preparation method thereof. The invention relates to a moisture-absorbing breathable polyester and chinlon composite fiber, which is a skin-core composite fiber, takes moisture-conducting polyester fiber as a skin layer, and takes water-absorbing modified chinlon fiber as a core layer, the moisture-conducting polyester fibers are prepared from the following components in parts by weight: 68 to 75 parts of superfine denier polyester filaments, 12 to 16 parts of seaweed extract powder, 4 to 6 parts of lyocell fibers and 5 to 11 parts of a moisture-conducting auxiliary agent; the moisture conducting auxiliary agent comprises cellulose nano fibrils, nano bamboo charcoal powder and sericin. Aiming at the problem that the moisture absorption and air permeability of the existing polyester-nylon composite fiber are difficult to meet higher industrialization requirements, the moisture absorption and air permeability type polyester-nylon composite fiber with high water absorption rate, short drip diffusion time and water evaporation speed is prepared.

The invention discloses a sericin low-hysteresis composite conductive hydrogel as well as a preparation method and application thereof, the hydrogel takes a copolymer of methacrylamide phenylboronic acid and acrylamide as a matrix, and the composite conductive hydrogel is formed by introducing polydopamine modified sericin and aluminum ions. In the hydrogel, a boric acid group on a main chain reacts with a phenolic hydroxyl group into which sericin is introduced, so that the hydrogel has excellent self-repairing capability, and a secondary structure of the sericin is changed from disorder to alpha-helix and beta-folding in the reaction, so that the hydrogel has excellent low hysteresis capability; the introduction of aluminum ions provides good conductivity for the hydrogel. A sensor prepared from the hydrogel can respond to the deformation of human body movement and monitor the resistance change during joint movement, so that the real-time monitoring of the movement state is realized. The preparation process is simple and convenient, the materials are green and environment-friendly, and the method has wide application prospects and is suitable for the fields of design of wearable sensors, sports motion correction and the like.

The invention relates to the technical field of mulberry silk fabric processing, and discloses a mulberry silk fabric precise temperature control degumming system and a method thereof.The method includes the following steps that firstly, silk fabric surface temperature data are collected through distributed temperature sensors, and a temperature distribution thermodynamic diagram is generated and output to a central controller; the method comprises the following steps of 1, obtaining a temperature distribution thermodynamic diagram, 2, constructing a three-dimensional temperature field prediction model based on the temperature distribution thermodynamic diagram obtained in the step 1, and calculating colloid dissolution activation energy difference values of all areas, and 3, dynamically dividing gradient temperature control areas according to the activation energy difference values obtained in the step 2, and generating target temperature curves and time parameters of all the areas. According to the invention, through cooperative regulation and control of the multi-channel execution mechanism, the problem of non-uniform temperature distribution in a traditional process is effectively eliminated, and the heating uniformity of the fabric surface is remarkably improved, so that selective removal of sericin is realized, and high consistency of degumming degree is ensured while fiber damage is avoided; the strict requirements of high-end silk products on the material integrity are met.

The invention discloses preparation and application of bacteriophage-loaded sericin microgel, and belongs to the technical field of food preservation. The sericin microgel capable of loading the bacteriophage is prepared from a sericin solution extracted by using 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) in combination with a mechanical shearing method and a high-temperature and high-pressure method. The sericin protein microgel loaded with bacteriophage, which is obtained by soaking the microgel into bacteriophage proliferation liquid after freeze-drying, can significantly improve the storage stability of the bacteriophage and can effectively prolong the shelf life of beef, and the preparation method is green, simple and safe, has a wide application prospect, and is suitable for industrial production. Meanwhile, a new field is opened up for efficient application of the bacteriophage.

The invention provides a wool fabric anti-pilling finishing agent and a preparation method, and relates to the field of fabric finishing agents, the wool fabric anti-pilling finishing agent comprises a dry material and a wet material, the dry material is obtained by adding a seaweed extract and oleuropein into a mixture of sericin and water and performing microwave treatment, low-temperature drying treatment and crushing treatment on the mixture, and the wet material is obtained by adding the seaweed extract and the oleuropein into the mixture. The wet material is obtained by mixing tea polyphenol, oleanolic acid and water. The anti-pilling finishing agent for the wool fabric, provided by the invention, is prepared from the sericin, the seaweed extract, the oleuropein, the tea polyphenol and the oleanolic acid materials, and the application of the natural materials not only is environment-friendly, but also can enhance the mechanical properties of the wool fabric while improving the anti-pilling property and the antistatic property of the wool fabric; after the anti-pilling finishing agent for the wool fabric is applied to the wool fabric, the wool fabric has the anti-pilling property and the durability.

The invention provides a method for separating sericin from silk degumming wastewater, which comprises the following steps: adding a cross-linking reagent into the silk degumming wastewater, uniformly mixing to obtain a mixed system, adding acid into the mixed system to adjust the pH value of the mixed system to 1.5-4.5, and keeping the mixed system at 30-80 DEG C for 10-90 minutes; adding a bridging reagent into the mixed system, uniformly mixing, standing to obtain a precipitate, and separating the precipitate to obtain the sericin, so that the problems that the sericin in the silk degumming wastewater of enterprises is difficult to recover, the cost is high, the COD value of the wastewater is high, secondary pollution is easily generated in the recovery process and the like at present are solved.

The invention relates to the technical field of food preservation, in particular to a double-layer fiber membrane for food preservation and a preparation method and application of the double-layer fiber membrane. The first layer of the double-layer fiber membrane for food preservation is composed of sericin / pullulan, and the second layer is a polylactic acid-based membrane doped with FA-coated Ag-ZIF-8 nanoparticles. The double-layer fiber membrane realizes high selectivity to oxygen and carbon dioxide, realizes regulation and control of microenvironment gas atmosphere, and inhibits respiration, so that an excellent fresh-keeping effect is achieved. The double-layer fiber membrane has antibacterial and antioxidant functions, and can inhibit oxidative degradation and microbial contamination of perishable fruits. According to the technology, dual mechanisms of gas regulation and control and biological protection are integrated, an innovative scheme is provided for solving the rotting problem of perishable fruits, and the technology shows important potential in the aspects of promoting development of a food fresh-keeping technology and optimizing a sustainable packaging scheme.

The invention belongs to the technical field of antibacterial materials, and particularly relates to a sericin / tannic acid-silver antibacterial-anti-biofilm nanocomposite as well as a preparation method and application thereof. According to the method, the TA-Ag nano material is prepared by adopting a simple water bath method; and then, dispersing SS in a TA-Ag aqueous solution to realize hydrogen bond combination of SS and TA so as to obtain the SS / TA-Ag nanocomposite with a stable structure. In the preparation process, plant polyphenol tannic acid is taken as a carrier, silver ions and tannic acid are combined by adopting a water bath method, and the antibacterial property of tannic acid is effectively improved; and the sericin is further modified through hydrogen bond crosslinking, so that the obtained composite material not only has antibacterial and anti-biofilm dual functions, but also is simple in preparation process and mild in reaction condition, and conforms to green chemistry and environment-friendly concepts.

The invention belongs to the field of biomedical materials, and particularly relates to a coagulation-promoting embolism microsphere and a preparation method thereof. The sericin-based embolism microspheres are creatively prepared, and the sericin membrane layer on the surface of the microspheres can prevent the risk that a catheter is solidified during an operation due to thrombin burst release during the operation. On the other hand, sericin in the microspheres has excellent water absorption swelling property and lubricity, and is beneficial to embolism and prevention of reflux; moreover, sericin molecules contain a large amount of hydroxyl and carboxyl, so that the chelation of blood coagulation factor calcium ions is greatly improved. In addition, thrombin factors are loaded into the microspheres, calcium ions are chelated on the surfaces of the microspheres, the microspheres are endowed with rapid embolism capacity through the superimposed effect, blood flow blocking of target blood vessels can be effectively accelerated, and reflux is prevented. The prepared procoagulant embolism microspheres are suitable for being applied to treatment of vascular-rich tumors of the brain and can effectively prevent mistaken closure of many complex functional blood vessels of the brain due to reflux.

This invention provides an antimicrobial peptide spray, its preparation method, and its application in the prevention and treatment of peristomal skin infections. The spray comprises, by weight percentage, 0.05%-0.5% of a complex antimicrobial peptide, 0.1%-5% of a moisturizing and repairing film-forming matrix, 0.05%-0.2% of a buffer, 0.8%-1.2% of an isotonic regulator, 0.01%-0.5% of a stabilizer, and 0.005%-0.05% of a metal ion chelating agent, with the balance being water for injection. The complex antimicrobial peptide is composed of bovine lactoferrin antimicrobial peptide, sericin oligopeptide, and buckwheat-derived anti-inflammatory active peptide in a weight ratio of (3-5):(2-3):1. This spray possesses four core functions: broad-spectrum antibacterial activity, inhibition of bacterial biofilm, anti-inflammatory and soothing effects, and skin barrier repair. It is less likely to induce bacterial resistance, does not irritate damaged skin around the stoma, and can effectively prevent and treat peristomal skin infections.

The invention discloses a scalp repair composition as well as a preparation method and application thereof. The scalp repair composition comprises sericin, ceramide NP, a purslane extract, polysorbate-20 and a water-phase matrix, the sericin and the ceramide NP are compounded to simulate a natural scalp cuticle structure, and barrier reconstruction is facilitated; the purslane extract is used for relieving pruritus and erythema in the repairing process; the pH value of the composition is 5.0-5.8, so that the composition is more suitable for the weakly acidic microenvironment of the scalp, and the irritation is effectively reduced. The composition can be applied to scalp repair essence, anti-hair loss products and medical auxiliary dressings, and has good safety and repair efficacy.

The present application relates to the technical field of neutral ink, and particularly relates to a sericin micro-nanoparticle neutral ink, a preparation method and application thereof, sericin micro-nanoparticles 0.05% to 20%, colorant 0.05% to 40%, additive 0.05% to 1.0%, antibacterial agent 0.05% to 1.0%, and the balance is water; the sericin micro-nanoparticles are prepared from sericin through glutamine transaminase crosslinking. The present application adopts glutamine transaminase crosslinking sericin protein to form sericin micro-nanoparticles as a film forming agent and a load stable base material, which is added into neutral ink, has a higher specific surface area, more hydrophobic groups and hydrophilic groups, so as to load and disperse biodegradable natural colorant through interface interaction, so that the neutral ink is functionalized, has higher colorant loadability, and meanwhile, the sericin micro-nanoparticles have excellent antioxidant property, can effectively protect biodegradable colorant, and improve the photo-thermal stability thereof.

The invention relates to a soft capsule, in particular to a long-acting DHA soft capsule and a preparation method thereof. The long-acting DHA soft capsule is prepared from the following raw materials in parts by weight: 10 to 11 parts of xanthan gum, 3 to 4 parts of locust bean gum, 0.5 to 1 part of sericin, 0.5 part of glycerol and 13.5 to 16 parts of deionized water. The preparation method comprises the following steps: stirring and heating to 75-80 DEG C until all the raw materials are dissolved in water or melted and solid particles visible to naked eyes do not exist, so that the raw materials are glue liquid; spreading the glue solution into a rubber sheet; pressing the capsule skin and the DHA grease composition into capsules through a soft capsule pelleting machine; after shaping, polishing and drying to obtain the long-acting DHA soft capsule. The capsule skin of the long-acting DHA soft capsule is of a single-layer structure, the structure is simple, the physicochemical property is determined, a traditional soft capsule pelleting machine can be used for pressing, an additional secondary coating process is not needed, and the production process is simplified; through cooperation of xanthan gum and locust bean gum, the gum skin keeps certain dimensional stability in a freezing environment.

The invention relates to the field of biomedical polymers, in particular to an antibacterial, compression-resistant and degradable pure sericin nano-composite gel dressing as well as a preparation method and application thereof. Main raw materials comprise sericin, magnesium lithium silicate, an initiator, a catalyst, an antibacterial agent and deionized water. The preparation method comprises the following steps: extraction of sericin, preparation of an inorganic nano-clay magnesium lithium silicate dispersion liquid, preparation of a gel pre-polymerization liquid, assembly of a sandwich model, injection of a sericin / magnesium lithium silicate gel pre-polymerization liquid, and preparation of the sericin / magnesium lithium silicate nano-composite gel. And carrying out post-treatment and packaging on the sericin / magnesium lithium silicate nano-composite gel dressing. The pure sericin protein hydrogel is prepared through interaction of hydrogen bonds and ionic bonds, the raw materials are non-toxic, and the pure sericin protein hydrogel has biocompatibility, high mechanical property, injectability, dissolvability and no pollution of dissolved products and can be used for wound dressing, drug delivery, biological binders, implantable electronic equipment and 3D biological printing.

The invention relates to the technical field of biological medicines, in particular to application of canthaxanthin extract and a feed additive containing the canthaxanthin extract in silkworm breeding, and finds that cocoon shell amount, whole cocoon amount and pupa weight of silkworms can be remarkably increased and egg laying amount of single moths can be increased by adding canthaxanthin extract to silkworms for the first time. Meanwhile, the silkworm variety with functional carotenoid binding protein can also be induced to produce orange red natural colored cocoons. The invention further provides a compound feed additive containing the canthaxanthin extract and the sericin-derived small peptide and / or gamma-aminobutyric acid. Experiments prove that the canthaxanthin extract and the sericin-derived small peptide have a synergistic effect in the aspect of increasing the cocoon shell amount. According to the technical scheme, on the premise that normal growth and development of silkworms are not affected, the multiple purposes of yield increasing, propagation promoting and color increasing are achieved at the same time, and the method has good application prospects and industrial value.

The utility model discloses a dehumidification structure and a dehumidification rotating wheel. The dehumidification structure comprises a moisture absorption medium layer, a sericin layer surrounding the peripheral side of the moisture absorption medium layer, a first connecting assembly and a second connecting assembly with elastic recovery capacity. The first connecting assembly comprises a plurality of fixed connecting columns capable of conducting heat, and the two ends of each fixed connecting column are connected with the moisture absorption medium layer and the sericin layer correspondingly; the second connecting assembly comprises a plurality of springback connecting columns; each springback connecting column penetrates through the moisture absorption medium layer in the thickness direction of the moisture absorption medium layer, and the two ends of each springback connecting column are connected with the corresponding sericin layers on the two sides respectively; according to the dehumidification structure, the moisture absorption performance can be improved by effectively utilizing the expansion characteristic of the moisture absorption medium layer, the expansion of the moisture absorption medium layer can be restrained to avoid excessive expansion, and the dehumidification structure can be used in the dehumidification rotating wheel.

The invention belongs to the technical field of wastewater treatment agents, and particularly relates to a preparation method and application of a tussah silk glue protein modified magnesium oxide adsorbent, and the preparation method is characterized by comprising the following steps: loading, separating, cleaning and drying, adding MgO into cocoon cooking wastewater, reacting in a gas bath constant-temperature oscillator under a reciprocating condition, uniformly dispersing magnesium oxide in the wastewater, and drying to obtain the tussah silk glue protein modified magnesium oxide adsorbent. Sericin is used as a modifier to be loaded on magnesium oxide; and drying the reactant precipitate, and grinding into fine powder to obtain the S-MgO adsorbent. The method has the beneficial effects that the viscous sericin in the cocoon cooking wastewater'wraps' and'bonds' magnesium oxide particles to form a composite aggregate, and the sericin and magnesium oxide functional groups are complementary, so that the pore structure of magnesium oxide is optimized, the methylene blue adsorption rate is as high as 90.30% which is far higher than the adsorption efficiency of only 11% of MgO before modification, and the adsorption efficiency of the magnesium oxide is greatly improved. More importantly, the sericin in the cocoon cooking wastewater is recycled, the purpose of treating waste with waste is achieved, and the application prospect is wide.

The invention discloses a hydrogel composite scaffold as well as a preparation method and application thereof. The hydrogel composite scaffold comprises a hydrogel matrix and drug-loaded microspheres loaded on the hydrogel matrix, the hydrogel matrix is grafted with a photosensitive group; the drug-loaded microsphere comprises a microsphere matrix and a drug loaded on the microsphere matrix; the hydrogel matrix and the microsphere matrix respectively and independently comprise natural protein and derivatives thereof; the natural protein and the derivative thereof comprise at least one of silk fibroin, sericin, collagen or gelatin. According to the invention, the natural protein and the derivative thereof are grafted and modified by adopting a photosensitive group, so that the injectable property can be realized, and the formed hydrogel matrix has good biological activity, can promote adhesion and proliferation of cells, and is combined with the drug-loaded microspheres to realize controlled release of drugs and promote regeneration and repair of damaged tissues. The hydrogel composite scaffold can be widely applied to preparation of biomedical materials, in particular to preparation of cartilage repair materials.

The application discloses a processing technology and cocoon silk, and processing is performed according to the following steps: S1: swelling dispersion and anti-sticking treatment: placing silk containing sericin in a weak acid buffer prepared from an organic acid, the pH value being 1.0-4.0, and performing swelling treatment by heating, unsaturated fatty acid is added in the weak acid buffer in advance or is added in the initial stage of swelling in step S1, S2: post-treatment: taking out the swelled silk and drying, and performing drying to obtain dispersed single-fiber cocoon silk which does not adhere to each other. Through the two-step cooperation of 'pH-temperature dominant swelling' and 'oleic acid preventing secondary adhesion', the prepared cocoon silk retains 60%-90% of natural sericin and is in a fluffy and dispersed single-fiber state; the cocoon silk replaces the traditional degumming process, reduces pollution, has high spinnability, and the retained sericin has excellent biological activity and biocompatibility, so that the cocoon silk material has higher competitiveness and can become a raw material of biomedical textiles.

The embodiment of the present application provides a preparation method of a composite hydrogel, comprising the following steps: S1, preparing methacrylated gelatin (GelMA); S2, separating a degumming solution of silk to obtain an HMSS solution, wherein HMSS refers to sericin with a molecular weight greater than 30kDa; S3, preparing the HMSS / GelMA composite hydrogel: dissolving the prepared GelMA in deionized water, adding a photoinitiator LAP to obtain a GelMA gel system, then mixing the GelMA gel system with the HMSS solution to obtain a pre-polymer mixture, and irradiating the pre-polymer mixture under ultraviolet light to obtain the HMSS / GelMA composite hydrogel; the HMSS / GelMA composite hydrogel prepared in this way has high porosity and good strength performance, and meets the application in the direction of tissue repair; and the present application further provides the composite hydrogel prepared by using the preparation method.

The application relates to a device for separating and extracting fibroin and sericin by high-pressure water vapor and an extraction method thereof. The device comprises a high-pressure outer cylinder, an outer cylinder sealing cover and an extrusion driving mechanism. An inner cylinder and a heater are arranged in the high-pressure outer cylinder. An inner cylinder sealing cover is arranged above the inner cylinder. The inner cylinder sealing cover is provided with a safety valve. The safety valve is used for automatically opening when the inner cavity of the high-pressure outer cylinder reaches a preset pressure, so as to connect the high-pressure outer cylinder and the inner cylinder. The heater is used for heating water to form water vapor. The inner cylinder is provided with a cocoon treatment cavity and a sericin storage cavity. The cocoon treatment cavity and the sericin storage cavity are connected through a solid-liquid separation channel. The sericin storage cavity is connected to the outside of the high-pressure outer cylinder through a liquid discharge pipe. The liquid discharge pipe is provided with an on-off valve. The cocoon treatment cavity is provided with an extrusion plate. The extrusion plate is provided with arrayed air holes. The extrusion driving mechanism drives the extrusion plate to extrude the cocoon treated by water vapor in the cocoon treatment cavity. The extruded liquid flows to the sericin storage cavity. The application improves the extraction rate of sericin.

The utility model discloses a silk sericin purification device, specifically relates to purification device technical field, including purification box, the purification box bottom wall fixed setting has main discharge pipe, two branch discharge pipes are connected to the main discharge pipe bottom, two branch discharge pipes outside wall all are fixedly set up with a connecting block, two connecting blocks bottom all rotatoryly connected with a rotating seat, rotating seat outside wall fixed setting has two connecting plates, two connecting plates are away from each other one end all are fixedly set up with a conversion seat, the filter core is arranged in the conversion seat, and the locking assembly is arranged between the connecting block and the rotating seat, through the setting of two branch discharge pipes, when the filter core replacement in a branch discharge pipe, the silk sericin of finishing purification is discharged through another branch discharge pipe, will not influence the main discharge pipe, branch discharge pipe silk sericin of finishing purification and discharge, improve the practicality of device.

The application belongs to the technical field of genetic engineering, and particularly relates to a gRNA targeting Bombyx mori sericin 1 gene, a method for preparing coarse silk and application. The target sequence of the gRNA is shown as SEQ ID NO:1. The method for preparing coarse silk comprises the following steps: 1) constructing a gRNA expression vector targeting the Bombyx mori BmSer1 gene, and preparing a gRNA transgenic Bombyx mori; 2) crossing the gRNA transgenic Bombyx mori and Cas9 transgenic Bombyx mori, and screening to obtain double transgenic individuals with green fluorescence in eyes and bodies; 3) screening to obtain a heterozygous mutant Bombyx mori strain with the BmSer1 gene knocked out through typing detection; and 4) obtaining coarse silk by feeding the transgenic Bombyx mori. The genetic editing technology is used to change the genetic traits of Bombyx mori, and the obtained silk has a larger diameter, a lower sericin rate, better water resistance and durability.

The utility model discloses a reeling end collecting device which comprises a mounting frame, and an end collecting assembly is arranged on the mounting frame. The filament collecting assembly comprises a cylinder fixed to the inner side of the mounting frame, the cylinder is of a C-shaped structure, the cylinder and an intercepting cover are spliced to form a circular structure, an opening is formed in one side of the cylinder, an access cover is rotatably connected to the inner side of the opening, water outlet holes are formed in the inner sides of the cylinder and the intercepting cover, and the water outlet holes are communicated with the cylinder. The water outlet holes are annularly distributed at equal intervals relative to the cylinder and the inner side of the intercepting cover, the intercepting cover is movably connected with the cylinder, a penetrating hole is formed in the cylinder, the intercepting cover is spliced to the top of the cylinder, the intercepting cover and the cylinder are each of a hollow structure, and one end of the intercepting cover and one end of the cylinder are connected with a first water inlet pipe and a second water inlet pipe respectively. According to the device, sericin on the surfaces of silk threads can be washed in the thread collecting process, the sericin remaining on the inner walls of the barrel and the intercepting cover is reduced, and the barrel and the intercepting cover are prevented from being blocked.

The invention relates to the technical field of vascular therapy, in particular to a nano-drug and a preparation method thereof.The nano-drug comprises a nano-carrier, a therapeutic drug and cross-linked protein are loaded on the nano-carrier, the cross-linked protein is at least partially loaded on the surface of the nano-carrier, and the cross-linked protein is at least one of silk fibroin, sericin and silk fibroin peptide; the therapeutic drug is at least partially loaded in the nano-carrier. On one hand, cross-linked protein on the surface of the nano-carrier is promoted to be cross-linked with protein in a blood vessel by initiating the photosensitive cross-linking agent, and the nano-carrier is anchored in the blood vessel, so that the risk that nano-drug particles are eluted from the blood vessel wall is greatly reduced; on the other hand, cross-linking of cross-linked protein on the surface of the nano-carrier can be promoted by initiating the photosensitive cross-linking agent, so that the surface of the nano-carrier is gelatinized, the slow release speed of the nano-drug anchored in the blood vessel is more lasting, and further effective drug concentration can be maintained for a long time, restenosis of the blood vessel is inhibited, and the treatment effect is improved.

The present application relates to a kind of judging methods of degumming state of silk, belong to silk fibroin production technical field, the degumming is to cut dry cocoon and put into degumming system and carry out degumming, by obtaining the first degumming liquid data and first silk data in the first degumming stage, obtain the second degumming liquid data in the second degumming stage, derive degumming liquid data change value, obtain second silk data based on degumming change value and first silk data, further judge the degumming state of silk, can only be determined by the conductivity of degumming solution and ultraviolet absorption peak value on-line judge degumming process corresponding degumming point silk fibroin degumming rate and silk fibroin molecular weight, can be used to monitor degumming progress, to control process to accurately control the molecular weight of silk fibroin, and suitable for alkaline degumming system and enzyme degumming system, provide universal judging standard, in the regulation silk fibroin molecular weight, cocoon raw material selection degumming process, screening cocoon raw material has important research significance and application value.

The invention relates to a stable sericin biopolymer composition comprising a sericin, a cross-linking agent and optionally a catalyst. The stable sericin biopolymer composition is prepared by freezing-thawing process. The process of cross-linking sericin using the freeze- thaw cycle does not require curing at high temperature or under strong pH thus preventing the damage to the sericin proteins. The stable sericin biopolymer is used for preparation of cost effective sericin substrate platform such as gels, films, scaffolds and electrospun films for 3D cell culture and other biomedical applications.