405 results about "SILK SERICIN" patented technology

The invention discloses natural silk facial mask base cloth which is made of a 100% natural silk filament sheet. The silk filament sheet is subjected to deoiling and softening treatment and then subjected to hot rolling by a roller so that sericin left on the surfaces of silk filaments can be fixed in a bonding mode, and then the natural silk facial mask is formed. The natural silk facial mask base material is of a filament structure and is good in transparency, water absorbency and water-retaining performance and soft and is closely attached to the skin without fiber tows; moreover, sericin protein left on the surface of silk has various beautifying functions of whitening, absorbing ultraviolet light and the like, and the natural silk facial mask base cloth can be used in cooperation with beauty fluid and can also be used independently, is an ideal material for making a beauty make and has good market prospects.

The invention discloses liquid shampoo. The liquid shampoo is prepared from, by mass, 50-60 parts of water, 0.5-1.0 part of a phase-A hair conditioner, 0.1-0.2 part of citric acid, 14-18 parts of a phase-B surface active agent, 0.1-0.2 part of a chelating agent, 0.1-1.0 part of a softener, 1-5 parts of a phase-B hair conditioner, 5-15 parts of a phase-C surface active agent, 0.1-0.5 part of an anti-dandruff agent, 2-5 parts of a phase-D surface active agent, 2-5 parts of a phase-E hair conditioner, 3-8 parts of a skin conditioner, 1-4 parts of a wetting agent, 0.5-1 part of a pearling agent, 0.1-0.5 part of an emulsion stabilizer, 0.2-0.4 part of essence, 0.2-0.4 part of a corrosion remover and 0.8-2 parts of sodium chloride. The skin conditioner is a compound of hydrolyzed sericin, olive oil PEG-7 esters, panthenol and a plant extract and has the effect of reducing pore plugging.

The invention relates to enhancer Hr3 adopting the nucleotide sequence shown in the SEQIDNO: 1. The enhancer Hr3 can be used for preparing and recombining heterologous protein by bombyx mori. The invention further relates to a recombination carrier containing the enhancer Hr3, and the recombination carrier is prepared in such a manner that the enhancer Hr3 is connected with a Nco1 restriction site at the upstream of a promoter of a carrier containing no enhancer through the Nco1 restriction site of the enhancer Hr3. In the invention, functional elements such as the first grade enhancer, activating transcription factor IE1, untranslated region sequences (5' UTR and 3' UTR) and the like which are expressed by intensifier are identified in the cellular level, and an efficient and stable sericin I expression system is built by integrating optimum elements on the basis, so that efficient expression recombination heterologous protein can be made of middle silk gland of transgenic bombyx mori.

The invention relates to the technical field of biomedicines, and in particular relates to a preparation and identification method of a domestic silkworm silk fibroin solution. The preparation method of the domestic silkworm silk fibroin solution sequentially comprises the following steps: (1) degumming, namely degumming domestic silkworm silks to remove silk sericin on the outer layer to obtain degummed silks; (2) dissolving the silks, namely dissolving the degummed silks by using a ternary solution containing calcium chloride to obtain a fibroin solution; (3) performing renaturation, namely sequentially performing dialysis treatment on the fibroin solution according to a concentration gradient from high to low by using a protein denaturant solution, and then performing water dialysis treatment to obtain a silk fibroin solution; and (4) storing, namely storing the silk fibroin solution. The invention also provides an identification method of the domestic silkworm silk fibroin solution. The method can be used for identifying the quality of the silk fibroin solution. The preparation method of the domestic silkworm silk fibroin solution disclosed by the invention is relatively high in economical performance, ensures that the molecular weight of the domestic silkworm silk fibroin solution is close to that of natural fibroin molecules, and is beneficial to the preparation of subsequent silk fibroin based biomaterials.

The invention discloses silk protein powder and a preparation method thereof. The preparation method comprises the following steps of: (1) degumming silks; (2) performing enzymolysis on silk fibroins and sericin; (3) separating and purifying silk proteins; and (4) pre-concentrating and drying a silk protein liquid. The silk protein powder disclosed by the invention comprises sericin proteins and silk fibroin proteins, has the advantages of high purity, high biological activity, proper molecular weight, white color, zero foreign smell and the like and can be used for making biological and medical materials, health protection food and the like, and the aims of pure nature, high activity, high functionality, rich nutrition and the like are fulfilled.

The invention discloses a high-quality mulberry silk manufacturing technology. The technology includes the specific steps of cocoon stripping, cocoon assorting, vacuum steaming, ultrasonic cooperative cocoon cooking, silk reeling, re-reeling, post-processing, testing, packaging, storage and the like. By means of the technology, cleanliness of silk is improved, and silk reeling quality is comprehensively improved, so that under the action of ultrasonic waves, the inside and the outside of a cocoon shell achieve the effects that sericin is uniformly swovlen, shell dropping on the inner layer is reduced, the reelability rate is improved, steaming efficiency is improved, work energy consumption is lowered, neatness and cleanliness of raw silk are improved, and fineness continuous dynamic non-contact detection of the raw silk is achieved. Quality of the raw silk is improved, raw silk drying efficiency is improved, and work energy consumption of equipment is lowered.

The invention relates to a preparation method for a carbon-film-coated zinc oxide hollow sphere. Pollen is taken as a template for preparing a ZnO hollow sphere, and sericin is used as a carbon source for performing carbon film coating; the condition does not exist before the application; the preparation method comprises the steps of fully mixing zinc salt with an ethanol solution of pollen, and adding alkali liquid to a water bath in a dropwise manner; performing aging, centrifuging and separating to obtain ZnO precipitate with a pollen template; performing high temperature sintering on dried white powder to obtain the ZnO hollow sphere; immersing the ZnO hollow sphere powder into a sericin solution, and performing centrifuging, separating and drying; and performing vacuum sintering on the dried powder to obtain the carbon-film-coated ZnO hollow sphere taking the sericin as the carbon source. The preparation method is low in cost, simple in process, low-carbon and environment-friendly; and the obtained carbon-film-coated ZnO hollow sphere can be used as a lithium ion battery negative electrode material, a photocatalytic material and the like.

The invention belongs to the technical field of nanomaterials and relates to a preparation method of silk fibroin-based nano MOF (metal-organic framework) materials. The MOFs in different nano shapes can be prepared through adjustment of the ratio of rare-earth ions to silk fibroin macromolecules. The preparation method of the silk fibroin-based nano MOF materials comprises steps as follows: silk fibers are degummed and then dissolved and dialyzed in a ternary solution prepared from calcium chloride, ethanol and water, a silk fibroin solution with certain concentration is prepared, under normal temperature or hydrothermal reaction conditions, the silk fibroin solution is induced by different amounts of the rare-earth ions or mixtures of the rare-earth ions and micromolecular polycarboxylic acid, and protein macromolecule-based MOFs adopting different nano structures are synthesized under the molecular self-assembly action. The preparation method is simple and easy to implement, raw materials are easy to obtain, and the silk fibroin-based MOF materials in various nano shapes can be prepared.

A method for preparing a sericin hydrogel, the method including: 1) weighing a cocoon of a fibroin-deficient mutant silkworm, Bombyx mori, extracting the cocoon by an aqueous solution of LiBr or LiCl, dialyzing an extracted solution to yield a sericin solution having a concentration of a non-degraded sericin of between 0.1 and 4 wt. %; and 2) concentrating the sericin solution to a concentration of between 1.5 and 10 wt. %, adding a crosslinking agent to the concentrated sericin solution at a ratio of between 2 and 500 μL of the crosslinking agent per each milliliter of the sericin solution, fully blending the crosslinking agent with the concentrated sericin solution, and keeping a resulting mixture at the temperature of between 4 and 45° C. for between 5 s and 36 hrs to yield a hydrogel.

The invention discloses a dyeing and finishing process of polyester and cotton blended fiber fabric. The process comprises pretreatment, presetting, modification treatment, dyeing, mordant removal, soaping, color fixing, after finishing, drying, sizing, calendaring and rolling. Snailase and alpha-amylase are adopted for pretreatment, modification treatment is adopted before dyeing, the polyester and cotton blended fabric is modified fundamentally, and the dye-uptake of the fabric is increased; in the dyeing procedure, fewer alkaline substances and inorganic salt are used, a dye leveler, substitution alkali, a migration inhibitor and a diffusant are added, the dye leveling performance is improved, natural dye is used, environmental pollution is avoided, and the environment-friendly requirement is met; self-cleaning treatment is added during aftertreatment, and the self-cleaning capacity, the crease-resistant performance, the wash durability and color fastness are improved; silk fibroin and sericin are adopted for afterfinish, and the anti-pilling performance and the antistatic performance of the fabric are provided; the whole process is environment-friendly and pollution-free.

The invention relates to sericin/oxidized graphene composite hdyrogel as well as a preparation method and application thereof. The preparation method comprises the steps: blending a methacrylic anhydride modified sericin solution and oxidized graphene, then adding a photoinitiator and performing crosslinking under ultraviolet irradiation to prepare sericin/oxidized graphene composite hydrogel withgood mechanical property and osteogenic induction performance. The prepared sericin/oxidized graphene composite hydrogel has the advantages of good biocompatibility and biodegradability, high mechanical strength and osteogenic induction and application to the aspects of medicine and cell carrying, tissue engineering repairing and the like.

The invention belongs to the technical field of textile engineering and provides a refining technology of silkworm oil rotten cocoons. The refining technology of the silkworm oil rotten cocoons is achieved through the following steps: primary refining cocoon boiling, biological decay, washing and dewatering, immersion cleaning, washing and dewatering, scouring and bleaching, washing and dewatering, secondary refining, washing and dewatering, softening, drying and cotton opening. By means of the refining technology, the biological decay enables silk fibers of the oil rotten cocoons to be fully dissociated and then mutually adhered together, accordingly short fibers are not prone to be omitted during later cocoon opening process, needle rollers are convenient to be hung, and use ratio of the silk fibers is improved. By adopting reasonable proportioning of various auxiliaries during different procedures, such as boiling refining, decaying, scouring and bleaching, damage to the silk fibers from chemical auxiliaries is reduced to the largest extent, oil removing effect is improved, sericin protective layers outside fibroins are reasonably protected, strength of the silk fibers is protected, comprehensive use ratio of oil rotten cocoons is improved, and the refining purposes including oil removing, rubber retaining, and fiber tensile force protection are achieved.

The invention discloses a preparation method of a green metal absorbing material with cellulose and sericin. The preparation method has the key points that the cellulose and the sericin protein of bamboo are utilized and the green metal absorbing material with high absorbing efficiency and full biodegradation is prepared by molecular design and chemical modification. The preparation method is simple, high-efficiency and no-pollution. The green metal absorbing material has the advantages that the cellulose and the sericin protein of the bamboo are introduced into raw materials for preparing the metal absorbing material, the range of the raw materials for preparing the metal absorbing material is expanded, an innovative idea for recycling the cellulose and the serich protein of the bamboo is provided, the breakthrough of the technical problem at the section of removing metal ions in industrial and domestic waste water is accelerated and great environmental and social benefits are achieved.

The invention relates to a composite protein peptide with skin care efficacy, a hydrogel thereof and a preparation method. The composite protein peptide comprises the following components by weight: 1.0-7.0 parts of silk fibroin peptide, 1.0-7.0 parts of casein peptide, and 0.1-2.0 parts of sericin. The composite protein peptide hydrogel comprises the following components by mass percentage: 0.5-20% of the composite protein peptide, 1.5-2.5% of an additive, 0.3-0.6% of a preservative, and the balance water, wherein the additive is one or more of xanthan gum, Carbomer, sodium alginate, and glycerin. The composite protein peptide and the hydrogel thereof provided by the invention have water supplementing, moisturizing, whitening, oxidation resisting and other skin care efficacy.

The invention discloses a reticular tissue engineering scaffold, which is characterized in that: it is mainly woven from sericin removed silk, the mesh size is 0.5-20mm<2>, and the composite biomaterial in the meshes is any of collagen, a medical polyester polymer synthetic material, chitosan, hyaluronic acid, and chondroitin sulfate. The reticular structure of the scaffold provided in the invention has both good mechanical properties and enough interlinked cell tissue accommodation space. The employed materials are easy to obtain, and have excellent mechanical and biological properties. The scaffold also has sufficient pores and good mechanical properties, and is suitable for repair and tissue engineering of ligaments, tendons, abdominal walls, pelvic floors and other tensile tissues.

The invention discloses a preparation method of fusiform nanomicelles, and solves the technical problems that at present, no self-assemblies with various morphologic characteristics and different properties are assembled from different proteins. The preparation method comprises the following steps: 1, preparing a sericin solution and an acid-process glutin liquid: adding sericin into deionized water to obtain the sericin solution with the mass concentration of 0.5-1.5%, and preparing the acid-process glutin solution with the concentration of 0.5-1.5% by using deionized water; 2, mixing the sericin solution and the acid-process glutin solution which are prepared in the step 1 to obtain a mixed solution, and adjusting the pH value of the mixed solution to be between isoelectric points of thetwo proteins; 3, assembling the fusiform nanocomposite micelles: uniformly stirring the mixed solution prepared in the step 2, and assembling at constant temperature for 12-18 hours to obtain the fusiform nanocomposite micelles. The composite micelles have good electrochemical stability, dilution stability and storage stability.

The invention discloses a silk fabric cold pad-batch pretreatment auxiliary. The silk fabric cold pad-batch pretreatment auxiliary comprises the following components in parts by weight: 10-25 parts of 8.5% hydrogen peroxide, 10-25 parts of sodium carbonate, 7.5-15 parts of sodium silicate, 40-46 parts of sodium dodecyl benzene sulfonate, 3-5 parts of polyoxyethylene lauryl ether sodium sulfate and 5-10 parts of sodium dodecyl sulfonate. Aiming at the particularity of the silk fabric, the invention provides a cold pad-batch pretreatment auxiliary which is more suitable for the silk fabric. By utilizing the silk fabric cold pad-batch pretreatment auxiliary, the silk fiber damage rate can be reduced to less than 0.6% simultaneously when various sericin impurities are effectively removed.

The invention provides a sericin nerve guidance conduit as well as a preparation method and application thereof. The sericin nerve guidance conduit contains sericin, and the preparation method comprises the following steps: grinding cocoons of fibroin-deleted mutant silkworms which serve as a raw material, sterilizing the ground cocoons by virtue of ultraviolet ray or ethanol, then extracting sericin by virtue of LiBr or LiCl water solution and preparing 0.5-20wt% of a sericin water solution from an extract; adding a covalent cross-linking agent to the sericin water solution, uniformly mixing and molding in a mold so as to obtain a semi-finished sericin nerve guidance conduit product, wherein the dosage of the covalent cross-linking agent is such determined that 2-500ul of the covalent cross-linking agent which is 0.1-50wt% in concentration is added to every 1ml of the sericin water solution; and freeze-drying the semi-finished sericin nerve guidance conduit product, so that the finished sericin nerve guidance conduit is obtained. The sericin nerve guidance conduit provided by the invention is good in bioactivity and biocompatibility; and the sericin nerve guidance conduit is naturally degradable in vivo and is capable of promoting the regeneration of peripheral nerves of long distant defect.

The invention discloses a method for environment-friendly in-situ synthesis of sericin-nano-silver by means of sericin and the application thereof. According to the method, a silver nitrate solution is added to a sericin solution for reaction under natural illumination till the mixed solution becomes yellow, so that the sericin-nano-silver is obtained. The sericin-nano-silver is synthesized in an in-situ mode under illumination based on the fact that the phenolic hydroxyl group in tyrosine has reducibility and can achieve silver ion reduction in a solution. Operation is easy, the condition is mild, and the method is environment-friendly. The prepared sericin-nano-silver has high dispersity and stability, has a good sterilization effect on staphylococcus aureus, and is low in cytotoxicity and high in biocompatibility. Therefore, the sericin-nano-silver prepared with the method has broad application prospects.