23results about How to "Ingredient safety" patented technology

The invention discloses a method for extracting a high-concentration seafood-flavor raw material from kelp and clam, which comprises the following steps of: crushing the kelp and clam, adding a proper amount of distilled water and protein compound enzyme into the kelp and clam, and performing enzymolysis under a certain enzymolysis condition; the preparation method comprises the following steps: taking papain, pancreatin, neutral protease and flavourzyme as enzymolysis combined liquid, performing enzymolysis and enzyme deactivation on the raw material homogenate, inoculating saccharomyces cerevisiae suspension into the enzymolysis liquid according to the inoculation amount of 5%, performing fermentation and fishy smell removal to obtain homogenate fermentation liquid, performing centrifugal filtration on the homogenate fermentation liquid, and performing ultrafiltration on the homogenate fermentation liquid. The extraction rate of the seafood flavor raw materials in the kelp and the clam is further improved through the excellent enzymolysis capacity of the enzymolysis combined liquid, so that the more thorough raw material utilization rate is achieved, the enzymolysis process of the compound enzyme is simple and easy to achieve, and the raw materials do not need to be subjected to other pretreatment in the using process.

The present invention provides a salmon mask with anti-inflammatory, anti-aging and repairing effects and a preparation method thereof, belonging to the field of cosmetic technology. The salmon mask includes the following components by weight: Phase A: 2-5 parts of aloe vera gel, 1-3 parts of sodium carrageenan, 2-4 parts of sodium alginate, 1-5 parts of konjac gum, 3-5 parts of glycerol and 85-95 parts of high-purity water; Phase B: 5-10 parts of polydeoxyribonucleotides, 10-25 parts of Centella asiatica extract, 10-25 parts of Portulaca oleracea extract, 5-15 parts of Calendula officinalis extract, 10-20 parts of hyaluronic acid, 2-5 parts of acetyl tetrapeptide-15, 1-3 parts of palmitoyl tetrapeptide-7, 1-3 parts of acetyl hexapeptide-8, 3-8 parts of conopeptide, 1-3 parts of snake venom tripeptide and 2-5 parts of pentapeptide-3. The present invention makes the obtained mask have better anti-inflammatory, moisturizing and repairing effects by selecting specific ingredients.

The invention provides a traditional Chinese medicine composition for treating pancreatic fibrosis and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition disclosed by the invention takes isoflavone as a main component, and the isoflavone not only can reverse the fibrosis degree of pancreatic fibrosis cells by retarding the proliferation and migration ability of fibrosis cells, influencing the cell activity and reducing the generation of various fibrosis proteins, but also can inhibit the illness state of mice with chronic pancreatic fibrosis; the disease progression is slow or blocked. According to the traditional Chinese medicine composition, the pancreatic fibrosis process is effectively reversed through retardation of isoflavone on pancreatic stellate cell fibrosis induced by TGF-beta1, the inhibition effect of the traditional Chinese medicine composition on pancreatic fibrosis can be improved in combination with other traditional Chinese medicine ingredients, and the ingredients are safe.

The invention discloses pseudomonas chlorophenolate with a carmine degrading function and application of the pseudomonas chlorophenolate, and belongs to the technical field of microorganisms. The preservation number of the pseudomonas chlorophenol is GDMCC (China General Microbiological Culture Collection Center) NO: 65476. The viable bacteria and cell-free extracts of the strain can degrade carmine acid, and have wide application prospects. The strain has the characteristics of broad-spectrum pH adaptability and no harm to the environment, has the advantages of being low in cost, safe in component, natural, energy-saving, environment-friendly and the like when being used for industrially preparing a microbial preparation, can be used as a potential microbial resource in treatment of waste water containing carmine acid dye, and provides a new way for treating the waste water through a microbiological method.

The invention belongs to the field of medical biology, and relates to a medical preparation, in particular to a nano supramolecular drug-loaded liposome and a preparation method thereof. The nano supramolecular drug-loaded liposome is prepared from the following raw materials in percentage by weight: 1 to 20 percent of active ingredient, 5 to 15 percent of lecithin, 20 to 40 percent of eutectic solvent, 0.1 to 10 percent of grease, 0.1 to 0.5 percent of cholesterol, 0.1 to 5 percent of emulsifier, 0.1 to 1.5 percent of stabilizer and the balance of deionized water, and the active ingredient is water-soluble VC (prototype VC). According to the invention, the active ingredients and the deep eutectic solvent are subjected to ultrasonic treatment to form a ternary supramolecular VC structure, and then the ternary supramolecular VC structure is wrapped, so that the stability of VC molecules in a solution state is greatly improved by virtue of dual clamping of supramolecules and lipidosome, the problem of oxidation discoloration of the VC molecules in a long-term storage process is improved, and the effect of VC in the cosmetic industry is better played.

The present application relates to a kind of salvianolic acid C freeze-dried preparation and preparation method and application, the salvianolic acid C freeze-dried preparation includes salvianolic acid C, pH adjusting agent, stabilizer and excipient.The salvianolic acid C freeze-dried preparation provided in the present application is traditional Chinese medicine extract, and each additive is suitable for administration route, component is safe, and good stability can be achieved, suitable for long-term storage, process is simple, reproducibility is good, easy to large-scale industrial production.The salvianolic acid C freeze-dried preparation of the present application can be injected to administration, also can be atomized to give human respiratory tract, provide a kind of new treatment means for anti respiratory tract influenza virus such as influenza virus A H1N1, influenza virus A H3N2, influenza virus B, parainfluenza virus, respiratory syncytial virus, adenovirus, etc., and have good curative effect.

The present invention discloses a bioactive peptide with antioxidant and hypoglycemic functions, a composition and an application, and belongs to the technical field of bioactive peptides. The amino acid sequence of the bioactive peptide with antioxidant and hypoglycemic functions of the present invention is shown in one of SEQ ID NO:1 to SEQ ID NO:4. The present invention combines a plurality of enzymes to hydrolyze defatted peanut meal, thereby improving the diversity of peptide segments, and obtaining highly active enzymatic hydrolysis products by optimizing conditions. The enzymatic hydrolysis products are separated and purified by ultrafiltration and high performance liquid chromatography-mass spectrometry, thereby ensuring the purity and structural clarity of the peptide segments. Screening is performed in combination with bioinformatics technology, thereby improving the activity and effect of the peptide sequence. In vitro and in vivo experiments have shown that the separated bioactive peptides have good antioxidant and hypoglycemic effects, and have broad application prospects in the preparation of antioxidant and hypoglycemic products.

The invention belongs to the technical field of polypeptide products, and discloses a method for preparing milt active peptide by combining compound enzyme treatment with supercritical carbon dioxide extraction, which comprises the following steps: respectively combining salmon seminal vesicle homogenate with protease to carry out restrictive enzymolysis reaction; carrying out enzyme deactivation on the obtained enzymatic hydrolysate, centrifuging, taking supernate, and drying the supernate; and performing deodorization treatment on the dry powder by adopting supercritical carbon dioxide extraction to obtain the milt active peptide. Compared with the prior art, the method has the advantages of mild extraction reaction conditions, few side reactions, high extraction efficiency, no damage to amino acid and easiness in control of hydrolysis degree. In addition, flavor optimization is carried out, nutritional ingredients of the protamine active peptide cannot be damaged, the extraction rate is high, the protamine active peptide has excellent rehydration performance and good water solubility, high functional characteristics are reserved, fishy smell and bitter taste are low, oxidation resistance is high, the protamine active peptide is more easily absorbed by the human body, the industrial application field of the protamine is widened, and the protamine active peptide is worthy of popularization and application. A new thought is provided for efficient development of protamine, and the method is suitable for industrial production.

The invention provides a water-based fire extinguishing agent and a preparation method thereof, and belongs to the technical field of fire extinguishing agents. The water-based fire extinguishing agent comprises a component A and a component B, the component A comprises potassium bicarbonate, potassium carbonate, potassium chloride and sodium acetate, and the component B comprises glycerin and water; the water-based fire extinguishing agent disclosed by the invention is clean in components, safe and pollution-free, can exert a remarkable fire extinguishing effect on general fire disasters, oil fire disasters, edible oil fire disasters and other fire disasters occurring in a home environment, and has very good practicability.

The invention relates to the technical field of external traditional Chinese medicine preparations, and provides plant anti-hair-loss hair-care hair-growing liquid and a preparation method thereof. The plant anti-hair-loss hair-care hair-growing liquid is prepared from the following raw materials in parts by weight: 15 to 20 parts of rhizoma zingiberis, 30 to 45 parts of radix polygoni multiflori, 5 to 15 parts of mulberry fruit, 25 to 40 parts of radix angelicae sinensis, 20 to 35 parts of folium mori, 5 to 10 parts of radix rehmanniae praeparata, 15 to 30 parts of cacumen biotae, 5 to 10 parts of fructus chaenomelis lagenariae, 18 to 25 parts of fructus ligustri lucidi and 100 to 120 parts of grain liquor. Various effective traditional Chinese medicine components have a synergistic effect, directly act on scalp hair follicles through transdermal use, help to improve scalp blood circulation, effectively moisten scalp and activate the hair follicles, play a role in consolidating hair roots and strengthening hair, can achieve the effect of treating both symptoms and root causes, are natural and safe in components, are suitable for various alopecia people to use, and have a good application prospect. The use requirements of people with alopecia are effectively met.

The invention relates to the technical field of food medicines and health care products, in particular to preparation and application of nanoparticles for effectively treating ulcerative colitis, which comprises the following steps: A, taking a diselenide compound as a raw material, and carrying out amidation reaction on the diselenide compound and N-alpha-fluorenylmethoxycarbonyl-N-epsilone-t-butyloxycarboryl-D-lysine to obtain Se-NHBoc; b, the Se-NHBoc is subjected to a deprotection reaction, Boc groups are removed, and Se-NH2 is obtained; and C, carrying out condensation reaction on the Se-NH2 and a carrier, and purifying to obtain the nanoparticles. The ROS-responsive nano-carrier has the beneficial effects that the constructed ROS-responsive nano-carrier is safe in component, has good drug controlled release ability, and can effectively resist gastrointestinal digestion, pH change and thermal degradation.

The invention provides a fresh-keeping solution for fresh-cut fruits and vegetables as well as a preparation method and application of the fresh-keeping solution. The fresh-keeping liquid comprises the following components in parts by weight: 0.06-1 part of limonene, 0.3-1 part of an emulsifier, 0.5-1 part of egg yolk lecithin, 10-21 parts of a chitosan aqueous solution, 4-16 parts of absolute ethyl alcohol and 160-185 parts of a weakly acidic buffer solution. When the fresh-keeping liquid provided by the invention is applied to the fresh-cut fruits and vegetables, an edible fresh-keeping film can be formed on the surfaces of the fresh-cut fruits and vegetables, evaporation of moisture on the surfaces of the fresh-cut fruits and vegetables can be reduced, respiration in tissues of the fresh-cut fruits and vegetables can be inhibited, the decay process of the fresh-cut fruits and vegetables can be delayed, and the components are safe and reliable.

The invention discloses a stable composition containing epigallocatechin gallate as well as a preparation method and application of the stable composition. The stable composition is prepared from glycerol, vitamin C, chlorogenic acid, ethyoxyl diethylene glycol and epigallocatechin gallate. The preparation method comprises the following steps: mixing glycerol and epigallocatechin gallate at room temperature, stirring and dispersing uniformly, heating to 75-80 DEG C, and stirring until complete dissolution to obtain a transparent solution which is a phase A; uniformly stirring vitamin C, chlorogenic acid and ethyoxyl diethylene glycol at room temperature to obtain a transparent solution which is a phase B; and uniformly mixing the phase A and the phase B to obtain the stable composition. The composition has the characteristics of high temperature resistance, illumination resistance and high stability, and the preparation method of the composition is simple and low in cost.

This invention discloses a pH-responsive silk fibroin-doxacin prodrug nanoparticle, its preparation method, and its application. Silkworm cocoons are degummed, dissolved in a lithium bromide aqueous solution, and freeze-dried to obtain regenerated silk fibroin powder. The regenerated silk fibroin powder is then combined with succinic anhydride, a condensing agent, adipic dihydrazide, and doxorubicin hydrochloride, and treated multiple times with a composite solvent to prepare an aqueous solution of the silk fibroin-doxacin covalently bound. This solution is then added dropwise to an antisolvent under stirring to induce self-assembly into nanoparticles. After centrifugation purification and resuspension in water, the product is obtained. This invention completely overcomes the shortcomings of traditional physical encapsulation methods of silk fibroin, which are prone to burst release. The nanoparticles are extremely stable in physiological blood environments and can rapidly break hydrazone bonds for precise drug release in the slightly acidic environment of tumor cell endosomes. The antisolvent granulation mechanism promotes the flipping of unreacted polar groups to the surface, giving the nanoparticles a moderately negative potential and rich active sites, resulting in excellent colloidal stability and deep functionalization potential.

The present invention provides a stomach-nourishing, digestion-aiding and anti-allergic medicinal tea and a preparation method thereof. The medicinal tea comprises the following raw materials according to weight: 10-15 parts of crape myrtle flowers, 15-20 parts of jasmine flowers, 10-15 parts of pomegranate flowers, 3-7 parts of white tea polysaccharides, 6-10 parts of coix seeds, 3-5 parts of hericium erinaceus, 1-3 parts of dried orange peel, 2-4 parts of yam, 2-6 parts of natto dry powder, and 1-3 parts of sodium citrate. The medicinal tea prepared by the present invention has stomach-nourishing, digestion-aiding and anti-allergic effects, and can comprehensively enhance the functionality of the medicinal tea and provide nutritional ingredients. The present invention utilizes crape myrtle flowers, jasmine flowers and pomegranate flowers as main active ingredients, and compounding other raw material components, the ingredients are safe, there is no additive, synergistic synergy, the prepared medicinal tea has no bitter taste, the flavor is enhanced, it is more popular with consumers, and has good market value.

The present invention discloses a plant extract with skin barrier repair efficacy, a preparation method and an application thereof. The plant extract is obtained by extracting Sargassum, Cyperus rotundus and Lycium barbarum with a supramolecular low eutectic solvent. The plant extract first replenishes moisture to the skin, instantly relieving the skin redness, itching, tingling and discomfort symptoms; then inhibits the expression of inflammatory factors, reduces the occurrence of inflammatory reactions, and can remove free radicals and some oxidation products, protect the skin from the attack of oxidative free radicals, and relieve skin discomfort; finally, rebuilds and strengthens the skin's natural protective layer, enhances the skin's own resistance and tolerance, and better copes with external stimuli and skin discomfort, thereby achieving a healthy state of the skin barrier from short-term relief to long-term maintenance, and preventing the recurrence of allergic reactions and damage to the skin barrier.

The present invention belongs to the field of cell culture technology, and specifically relates to a cryopreservation protection solution and a cryopreservation method for adipose mesenchymal stem cells. The cryopreservation protection solution for adipose mesenchymal stem cells includes a basal liquid, a nutrient agent and an antifreeze agent; the basal liquid is: a culture medium; the nutrient agent is: transferrin, linoleic acid, cholesterol; the antifreeze agent is: hydroxyethyl starch, bergenia lactone, geraniol. The cryopreservation protection solution provided by the present invention adds a nutrient agent and an antifreeze agent to the basal liquid culture medium, especially the added antifreeze agents hydroxyethyl starch, bergenia lactone, and geraniol, which can effectively reduce the damage caused to cells during the cryopreservation process, improve the activity of frozen cells after recovery, and effectively ensure that the adipose mesenchymal stem cells after cryopreservation can still be used in scientific research or clinical practice. The present invention also provides a cryopreservation method for adipose mesenchymal stem cells, which is simple in process and easy to operate.

The invention discloses application of the composition. The composition comprises egg oil, semen raphani oil, perilla seed oil and the like, and can remarkably improve behavioral performance of an Alzheimer's disease model, hippocampal injury of the Alzheimer's disease model, inflammation and oxidative stress, hippocampal acetylcholinergic transfer of the Alzheimer's disease model and intestinal inflammation of the Alzheimer's disease model. According to the present invention, the egg yolk oil composition has the characteristics that the egg yolk oil composition has the characteristic that the egg yolk oil composition is close to the existing chemical drug donepezil for treating the Alzheimer's disease in a plurality of detection indexes, the egg yolk oil composition is significantly superior to the donepezil in the cognitive index index, and the egg yolk oil composition has the cognitive disorder treatment effect, and is superior to the existing drug donepezil to a certain extent.

The invention relates to a method for extracting elastin peptide from jellyfish. The method comprises the following steps: S1, washing fresh jellyfish with clear water to remove impurities or soaking and salting the jellyfish with cold water containing citric acid / acetic acid to desalt; cutting into small blocks for later use; s2, mixing the jellyfish with a sodium hydroxide solution with the pH value of more than or equal to 11.5, carrying out pressure maintaining and shearing treatment by using a high-pressure shearing machine, centrifuging, and retaining precipitates; s3, mixing the precipitate with pure water, adjusting the pH value to be less than or equal to 1.5 by using hydrochloric acid, reacting for 1-4 hours, and centrifuging to reserve the precipitate; s4, the precipitate is mixed with sodium chloride saline water, the concentration of the sodium chloride saline water is 0.8-1.5 M, a reaction is conducted for 1-4 h, centrifugation is conducted, and the precipitate is reserved; s5, mixing the precipitate with pure water, adjusting the pH value to 6.8-7.4, adding elastinase for enzymolysis, performing high-speed centrifugation, and retaining the precipitate; and S6, collecting the precipitate to obtain the jellyfish elastin peptide. The jellyfish is used as a raw material and has the advantages in the aspects of raw material cost, availability, pretreatment and the like.

The invention provides an autolysis protective film triggered by double conditions in a wading environment and a preparation method of the autolysis protective film. The autolysis type protective film comprises an inner film layer and a composite hydrophobic barrier layer, wherein the inner film layer comprises a dynamic cross-linked network and a mechanical sensitive microcapsule; the composite hydrophobic barrier layer is composed of a hydrophobic agent sprayed or impregnated on the surface of the membrane; the preparation method comprises the following steps: firstly preparing the inner membrane layer and then carrying out hydrophobic barrier treatment; the protective film triggers autolysis under the double conditions of a wading environment and mechanical vibration with the frequency of 50-200 Hz and the amplitude larger than or equal to 0.2 mm, false triggering of the natural wading environment is avoided, and the protection reliability is improved. After being triggered, the microcapsule releases a decrosslinking agent, so that the inner membrane layer is promoted to be quickly dissolved, manual cleaning is not needed, the coating is suitable for underwater temporary protection and other scenes, and the barrier layer is safe, easy to degrade and environmentally friendly; the preparation method is simple in process, easy to operate, beneficial to industrial production, capable of improving efficiency and capable of reducing cost.

The invention relates to the technical field of biological medicines, in particular to a novel compound with efficient mosquito-repelling and itching-relieving effects, the compound is composed of bioactive peptide and N, N-diethyl-2-hydroxy-2-phenylacetamide, and the bioactive peptide and the N, N-diethyl-2-hydroxy-2-phenylacetamide are combined to form the compound. Wherein the bioactive peptide comprises peptide fragments of which the sequences are as follows: SEQ NO: 1: Cys-Arg-Val-Leu-Pro (CRVLP), SEQ NO: 2: Gly-Asn-Phe-Thr-Ser (GNPTS) and SEQ NO: 3: Lys-Met-His-Glu-Tyr (KMHEY). The invention also discloses a preparation method of the bioactive peptide. The used components are relatively high in safety, so that the potential skin adverse reaction risk is reduced. The bioactive peptide is composed of natural amino acids, and has good biocompatibility and low toxicity. Safety evaluation on the N, N-diethyl-2-hydroxy-2-phenylacetamide shows that the N, N-diethyl-2-hydroxy-2-phenylacetamide has small harm to human skin and body under normal use concentration, and has a remarkable effect on relieving itching.

The application discloses a detection reagent and a detection method for fermentation power and acid production capacity of Daqu, and relates to the technical field of analysis and detection. The detection reagent comprises a sugar composition and an acid. The sugar composition is composed of glucose, maltose and sucrose, and the mass ratio of the glucose, the maltose and the sucrose is (4-18):(1-5):(0.1-1). The acid is at least one selected from acetic acid and lactic acid. The detection reagent of the application comprises glucose, maltose, sucrose and acid, and the acid is at least one selected from acetic acid and lactic acid. The application simplifies the components of the detection reagent, has the advantages of safe components, controllable safety, strong applicability and wide coverage, and makes the evaluation of the fermentation power and the acid production capacity of Daqu more accurate and efficient.

The invention provides application of a ficus pumila fruit extract in improvement of allergic asthma, and belongs to the technical field of traditional Chinese medicines. According to the present invention, the expression levels of mitogen-activated protein kinase (MAPK) and NF-[kappa] B pathway related proteins are adjusted by using the ficus pumila fruit extract so as to alleviate or improve the allergic asthma symptom. The ficus pumila fruit extract is a ficus pumila fruit aqueous extract or ficus pumila fruit polysaccharide, and can reverse abnormality of organ indexes of mice with allergic asthma, recover integrity of weight and intestinal structures of the mice and slow down oxidative stress reactions in intestinal tracts of the mice, including reduction of SOD in the intestinal tracts, improvement of MDA level and reduction of inflammatory factor level in alveolar lavage fluid. Comprise the concentration of TNF-alpha and the levels of interleukin-5 and interleukin-13, and the curative effect on allergic asthma is remarkable.