Cell preservation solution and preparation method and applications thereof

A preservation solution and cell technology, applied in the field of clinical medicine, can solve the problems of unsatisfactory preservation efficiency of mesenchymal stem cells, complex components, cumbersome preparation process, etc., to achieve excellent maintenance effect, maintain biological characteristics, improve The effect of the treatment effect

Active Publication Date: 2015-04-29
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition of the preservation solution is complicated, the preparation process is cumbersome, and it is only suitable for the preservation of mesenchymal stem cells, and cannot be used to maintain the vitality of immune cells. At the same time, the preservation efficiency of mesenchymal stem cells has not achieved satisfactory results.

Method used

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  • Cell preservation solution and preparation method and applications thereof
  • Cell preservation solution and preparation method and applications thereof
  • Cell preservation solution and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Preparation of Example 1 Cell Preservation Solution

[0071] Take the compound electrolyte injection and the compound amino acid injection and mix them in a mass ratio of 1:1 to form a preservation base solution.

[0072] Add albumin, glucose and vitamin C to the preservation base solution prepared above, the mass percentage of albumin is 1%, the mass percentage of glucose is 5%, and the mass percentage of vitamin C is 0.5% , and mix well to make a cell preservation solution.

[0073] Put the cell preservation solution prepared above into a sterile empty box, transfer the empty box filled with the cell preservation solution to a storage box with an ice pack, and transport it at low temperature (0-4°C); or put The cell preservation solution prepared above was put into a sterile container and stored in a refrigerator at 0-4°C.

Embodiment 2

[0074] Preparation of Example 2 Cell Preservation Solution

[0075] The compound electrolyte injection and the compound amino acid injection were mixed at a mass ratio of 1:1.5 to form a preservation base solution.

[0076] Add albumin, glucose and vitamin C to the preservation base solution prepared above, the mass percentage of albumin is 3%, the mass percentage of glucose is 8%, and the mass percentage of vitamin C is 0.1% , and mix well to make a cell preservation solution.

[0077] Put the cell preservation solution prepared above into a sterile empty box, transfer the empty box filled with the cell preservation solution to a storage box with an ice pack, and transport it at low temperature (0-4°C); or put The cell preservation solution prepared above was put into a sterile container and stored in a refrigerator at 0-4°C.

Embodiment 3

[0078] The preparation of embodiment 3 cell preservation solution

[0079] Take the compound electrolyte injection and the compound amino acid injection and mix them at a mass ratio of 1:2 to form a preservation base solution.

[0080] Add albumin, glucose and vitamin C to the preservation base solution prepared above, the mass percentage of albumin is 5%, the mass percentage of glucose is 3%, and the mass percentage of vitamin C is 0.3% , and mix well to make a cell preservation solution.

[0081] Put the cell preservation solution prepared above into a sterile empty box, transfer the empty box filled with the cell preservation solution to a storage box with an ice pack, and transport it at low temperature (0-4°C); or put The cell preservation solution prepared above was put into a sterile container and stored in a refrigerator at 0-4°C.

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Abstract

The invention relates to the technical field of clinical medicines, and particularly relates to a cell preservation solution and a preparation method and applications thereof. The cell preservation solution comprises albumin, glucose, vitamin C and basic preservation liquid, wherein the basic preservation liquid is a mixture of compound electrolyte injection and compound amino acid injection. The cell preservation solution disclosed by the invention achieves a good maintaining effect on the viability and morphology of more than two kinds of seed cells, and can preserve the seed cells for a long time, and maintain the biological characteristics of the seed cells, thereby significantly improving the therapeutic effect of the seed cells.

Description

technical field [0001] The invention relates to the technical field of clinical medicine, in particular to a cell preservation solution and its preparation method and application. Background technique [0002] In recent years, cell therapy has gradually replaced traditional drug therapy and played an important role in various diseases. Cell therapy refers to using the characteristics of certain cells with specific functions, using bioengineering methods to obtain and / or through in vitro expansion, special culture, etc., to make these cells have the ability to enhance immunity, kill pathogens and tumor cells, and promote tissue regeneration. Therapeutic effects such as organ regeneration and body rehabilitation, so as to achieve the purpose of treating diseases. Among them, mesenchymal stem cells and immune cells have become the seed cells for disease treatment. [0003] Mesenchymal stem cells (MSCs) are a group of mesoderm-derived pluripotent stem cells with self-renewal a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 葛啸虎陈海佳王一飞卢瑞珊王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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