47results about How to "Maintain biological characteristics" patented technology

The invention relates to the technical field of clinical medicines, and particularly relates to a cell preservation solution and a preparation method and applications thereof. The cell preservation solution comprises albumin, glucose, vitamin C and basic preservation liquid, wherein the basic preservation liquid is a mixture of compound electrolyte injection and compound amino acid injection. The cell preservation solution disclosed by the invention achieves a good maintaining effect on the viability and morphology of more than two kinds of seed cells, and can preserve the seed cells for a long time, and maintain the biological characteristics of the seed cells, thereby significantly improving the therapeutic effect of the seed cells.

The invention discloses an extraction method of filament eucommia rubber from eucommia leaves and peel; the method uses petrol ether as solvent to extract the eucommia rubber and has the steps: first, the eucommia leaves or the peel is made into small pieces and added with NaOH solution to dissolve cuticle; then cellulase is added to hydrolyze cell wall; the petroleum ether solvent at 60 DEG C to 90 DEG C of boiling range is added; then the obtained solution is extracted through recirculation at the temperature of 85 DEG C and filtrated when being hot, then cooled and frozen; the filament eucommia rubber is produced after filtration. Concentration of the NaOH solution is 0.5 percent to 1.5 percent and the dissolution time is 6 hours; pH value of the cellulase used for hydrolyzing the cell wall is 4 at temperature of 50 DEG C; enzymatic hydrolysis time is 1 hour to 2 hours and the time for reflux extraction is 2 hours; the frozen time is 30 minutes to 60 minutes at temperature of minus 20 DEG C to 0 DEG C. The method can effectively and completely extract the eucommia rubber to make original eucommia rubber and keep the original biological characteristics and physical properties and status and the original molecular structure and polymerization of the eucommia rubber; the method uses the cellulose to extract pretreated eucommia leaves raw materials, and gets the eucommia rubber of high quality when getting high yield.

The invention discloses a mesenchymal stem cell serum-free culture medium. The mesenchymal stem cell serum-free culture medium comprises a basic culture medium and added ingredients added in the basic culture medium, and the added ingredients comprise L-glutamine, nonessential amino acid, L-ascorbic acid, sodium selenite, fibronectin, ethanolamine, hydrocortisone, a trypsin inhibitor, human transferrin, human insulin, bFGF, TGF-beta1 and PDGF-BB. By means of the culture medium, the problems that in the prior art, the serum-free culture medium is poor in cell adherence, components are relatively complex, and primary cell culture is not supported are solved.

The invention relates to the field of medicines, in particular to a composition and an application thereof, a placenta preservative and a preparation method of the placenta preservative. Umbilical cord plasma is adopted as a nutrient of a placenta-derived mesenchymal stem cell preservation solution for the first time, so that multi-element nutrient substances are provided for seed cells; the cell viability is maintained; and the original cell morphology and biological characteristics of placenta-derived mesenchymal stem cells can be well kept. Due to the addition of reducing agents such as N-acetylcysteine and vitamin C, and an antioxidant, the resistivity of the placenta-derived mesenchymal stem cells is greatly improved; and the vitality of the placenta-derived mesenchymal stem cells can still be well kept within 24 hours.

The invention relates to the technical field of cell culture, and particularly relates to a dental pulp mesenchymal stem cell cryopreservation solution and a cryopreservation method thereof. The cryopreservation solution is a conditioned medium containing DMSO, FBS and dental pulp mesenchymal stem cells; a preparation method of the dental pulp mesenchymal stem cell conditioned medium includes the steps of conducting cell culture of dental pulp mesenchymal stem cells by using a cell culture medium, and after cell confluence degree reaches 80%-90%, collecting supernatant in the cell culture medium to obtain the dental pulp mesenchymal stem cell conditioned medium. The cryopreservation solution is adopted for cryopreservation of the dental pulp mesenchymal stem cells, the damage effect on the dental pulp mesenchymal stem cells by the cryopreservation solution is thus greatly reduced, cell viability of the dental pulp mesenchymal stem cells in cryopreservation process and thawing and waking process are improved, and biological characteristics of the dental pulp mesenchymal stem cells can be better maintained.

The present invention discloses a vacinium bracteatum leaf freeze-dried powder, its preparation method and application. Said preparation method includes the following steps: collecting vaccinium bracteatum leaves, sorting, cleaning, breaking, pressing to obtain vaccinium bracteatum leaf juice, filtering, removing impurity, making centrifugal purification, concentrating, vacuum freeze-drying to obtain dried solid, pulverizing to obtain the invented vaccinium bracteatum leaf freeze-dried powder. It can be directly made into various health-care foods of vaccinium bracteatum wine and vaccinium bracteatum beverage, etc.

The invention discloses an environment-friendly microalgae collecting method. According to the method, microalgae is cultured into the concentration of 0.5 g/L-1.5g/L in a microalgae bioreactor, 80% of a microalgae culture solution is transferred into a flocculation reaction tank through a pipeline or a pump, a cationic starch solution with a substitution degree of 0.15-0.45 is added into the microalgae culture solution and is taken as a flocculating agent, the wet to dry weight ratio (w/w) of a cationic starch mass to the microalgae in the solution ranges from 0.01 to 0.20, the microalgae culture solution is stirred for 5-10 minutes at the rotation speed of 200 r/min to promote the microalgae to flocculate, the microalgae culture solution is subjected to static settlement for 10-30 minutes, a sedimentary microalgae concentrated solution is collected, and a liquid supernatant is circularly used for re-cultivating the microalgae. According to the environment-friendly microalgae collecting method, the cationic starch solution is taken as the flocculating agent, so that the method has the characteristics of non-toxicity, economy, environmental protection, high flocculation efficiency and the like, flocculated microalgae is dense, the enrichment factors are high, original biological characteristics of the microalgae can be better kept, and the microalgae can be directly used in the fields of food, feed, biodiesel and the like.

The invention discloses a preparation technology of Gu Chisan for treating periodontitis. Gu Chisan comprises tortoise shell, halitum, Ligusticum wallichii, Cyperus Rotundus L, lotus leaf, Chinese prickly ash, hibiscus syriacus linn and dahurian angelica root. The process includes: A. pretreating raw medicinal materials; B. pulverizing the medicinal materials, i.e. pulverizing the dried net medicinal materials that are cooled to room temperature respectively in a rough grinding machine into rough powder, and sieving the powder through a sieve of 80 meshes; C. disinfecting the rough powder of all medicinal materials with oxirane; D. due to a high water content of the disinfected medicinal rough powder, drying the rough powder of all medicinal materials in a baking oven to a water content of 5.0-6.0%; E. pulverizing and sieving, i.e. pulverizing the dried rough powder of all medicinal materials respectively into finest powder in a fine grinding machine, and sieving through a sieve of 120 meshes so as to obtain finest powder of each medicinal materials; F. weighing, proportioning and packaging: weighing and charging the finest powder of each medicinal material based on a mass ratio of the components, mixing the finest powder overall; charging separately and packaging hermetically the mixed finest powder, thus obtaining a finished product. The preparation technology is characterized by high degree of mechanical automation, further guaranteed quality, low labor intensity and high production efficiency.

The invention discloses a method for extracting and separating eucommia seed oil and eucommia gum from eucommia samaras. The method comprises the following steps: processing the eucommia samaras to separate shells from kernels; performing steam explosion, enzymolysis, extracting and purifying on the shells to obtain the eucommia gum; crushing, extracting and separating the kernels to obtain the eucommia seed oil. In the method, the eucommia samaras are taken as raw materials for extracting the eucommia gum and the eucommia seed oil, and a membrane technology is introduced for implementing separation, so that the product quality and the yield can be improved, impurities can be fully removed through filtering, the needed time is short, and the extraction and separation temperature is low. The steam explosion is performed on the shells of the eucommia samaras for extracting and separating the eucommia gum, the eucommia seed oil is extracted by a supercritical CO2 technology, and residues obtained after extraction of the eucommia seed oil are used as raw materials of a bio-organic fertilizer, so that comprehensive development and utilization of the eucommia samaras are effectively realized, the cost during the development process of the eucommia gum is reduced and a high-value utilization way of the eucommia samaras is developed.

The invention discloses a production method for processing red wine by using lotus roots, which relates to a production process for red wine. The method comprises the following steps of: preparing the clean lotus roots into lotus root slurry, washing and filtrating, and taking starch slurry; and mixing the lotus root slurry, the starch slurry and high-temperature amylase and heating the mixture till the starch slurry is fully dissolved, then cooling the mixture to between 30 and 35 DEG C, adding saccharifying enzyme into the mixture, putting the sugar solution into a fermentation vat, adding pure yeast wine into the fermentation vat, stirring the solution uniformly and then sealing the solution, standing the solution for 8 to 10 hours at the environmental temperature of between 26 and 32 DEG C, keeping the temperature in the fermentation vat between 28 and 35 DEG C, and pumping the fermented solution into a post-fermentation tank after 9 to 11 days for storage and ageing. Without special additive processing such as color protection and the like, the product has peculiar aroma and smell of the lotus roots, bright luster, unique flavor and rich nutrition, most important, keeps all water-soluble substances and nutritional components of the lotus roots, and can assist digestion, strengthen spleen and stomach, reduce fat, enrich blood, nourish blood promote tissue regeneration and keep healthy after drinking all the year round.

The invention discloses an oral rhizoma polygonati edible-fungus liquid for resisting fatigue and improving the immunity and a preparation method thereof. The oral liquid adopts pure natural food materials including myrtle fruits, hericium erinaceus, cordyceps sinensis, tremella, agaric, lentinus edodes, cordyceps militaris, poria cocos, polyporus umbellatus and phellinus linteus as raw materialsfor extraction and concentration to obtain an extractive solution, and then the extractive solution is mixed with rhizoma polygonati nano-powder and subjected to homogenization, sterilizing, encapsulation and the like to obtain the oral rhizoma polygonati edible-fungus liquid with the functions of improving the immunity and resisting fatigue. The oral liquid also has the effects of replenishing blood, invigorating qi, nourishing yin, invigorating the kidney, moistening the lung, promoting salivation and the like and has a wide application range. People of all ages and sexes can drink the oralliquid. The preparation method is simple, easy to operate and environmentally friendly and can be used for industrial production.

The invention relates to the medical field, particularly to a composition and its use, an umbilical cord preservation preparation and a preparation method thereof. According to the invention, physiological saline is taken as the basic ingredient of umbilical cord preservation liquid, umbilical cord blood plasma is used as the nutritional ingredient source, as a natural substance, umbilical cord blood plasma not only provides multiple nutritional ingredients, but also can maximumly maintain the umbilical cord vitality in order to maintain a microenvironment similar to that in vivo. Lactobionic acid, hydroxyethyl starch and other macromolecular substances are added to avoid swelling death of the umbilical cord in the preservation process. The preservation liquid can be used for transportation and preservation of the preparation at low temperature. Thus, adequate nutrients are supplied to in vitro umbilical cord, and also the seed cell vitality, the original cell morphology and biological characteristics are well maintained.

The invention provides a process method and equipment for preparing activated sludge-based granular biochar based on a one-step method, and relates to the technical field of sludge recycling. A separating and discharging component is mounted on the feeding device; the feeding device is fixedly connected with a reciprocating driving part; a scattering device is mounted on the feeding device; the bottom of the feeding device is fixedly connected with a deodorization inorganic separation part; a dehydration device is mounted at the bottom of the deodorization inorganic separation part; a diversion mounting part is fixedly connected to the side of the dewatering device; the flow guide mounting part is fixedly connected to the bottom of the deodorization inorganic separation part; a processing unit is mounted in the flow guide mounting part, inorganic substances in sludge can be effectively removed, meanwhile, the rapid dehydration performance is achieved, discharging is uniform, the deodorization effect is better, and the problems that existing preparation equipment is poor in deodorization effect, inorganic substance separation and sludge pre-dehydration effects cannot be rapidly integrated, consequently, the dehydration efficiency is reduced, and the sludge pre-dehydration effect is poor are solved. And the overall application range is small. The stable and high-efficiency preparation of the activated sludge-based granular biochar is realized.

The invention discloses a process for extracting coenzyme Q10 from tobacco leaves. The process comprises the following steps: 1) pretreatment, to be specific, extracting abandoned inferior tobacco leaves to obtain a tobacco leaf extract, adding purified water by equal amount, putting into a high pressure tank, pressurizing to 4kg/cm<2> such that the pressure is uniform, maintaining for 5 to 10min, then recovering to normal pressure within 1 to 3s to obtain a material, and then carrying out saponification treatment on the material, and crystallizing and filtering to obtain a solanesol-containing extract; 2) molecular distillation, to be specific, carrying out molecular distillation on the solanesol-containing extract obtained in the step 1) to remove impurities to obtain light-phase solanesol and heavy-phase solanesol; 3) column chromatography separation, to be specific, dissolving and filtering the heavy-phase solanesol obtained in the step 2) with acetone, carrying out column chromatography separation, collecting eluant and concentrating the eluant to obtain solanesol subjected to molecular distillation and purification; 4) preparation of methyl dimethoxy benzoquinone, to be specific, carrying out brominated etherification and oxidation on methylphenol to obtain the methyl dimethoxy benzoquinone; 5) preparation of the coenzyme Q10 by mixing the light-phase solanesol obtained in the step 2), the solanesol subjected to molecular distillation and purification and obtained in the step 3) and the methyl dimethoxy benzoquinone obtained in the step 4), and reacting.

The invention discloses a human cell culture medium which comprises amino acids, vitamins, inorganic salts, hypoxanthine, thymidine, glucose, sodium pyruvate, linoleic acid, lipoic acid, putrescine and 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid. Due to improvement on components of the culture medium, according to the growth and metabolism characteristics of a human cell line, components such as pyridoxine hydrochloride, copper sulfate, ferrous sulfate, zinc sulfate, magnesium chloride, disodium hydrogen phosphate, sodium hypoxanthine, linoleic acid, lipoic acid, putrescine, thymidine and the like are increased, a microenvironment for growth of cells in the human body is met, and synthesis of human cell bio-enzymes can be promoted. The concentration of each component in the culture medium is optimized, the instability brought by a novel component is reduced, biological characteristics of the cultured cells are well maintained, and usage of serum during cell culture can be reduced or avoided. According to the test, the culture medium can be suitable for adherent culture and suspension culture and is applicable to the culturing of most of the human cells.

The invention provides a 3D suspension culture method for in-vitro amplification of spermatogonial stem cells. The method comprises the following steps: inoculating a spermatogonial stem cell suspension on RGD polypeptide modified porous polylactic acid microspheres in a rotary biological reaction container, adding an improved RPMI-1640 culture medium, carrying out three-dimensional static cultureat 30-34 DEG C, starting a rotary system after the spermatogonial stem cells adhere to the RGD polypeptide modified porous polylactic acid microspheres, and carrying out three-dimensional dynamic suspension culture at 30-34 DEG C. By using the method disclosed by the invention, in-vitro large-scale amplification of the mouse SSCs can be realized on the premise of not adding feeder layer cells, and good stem cell proliferation activity and undifferentiated activity are obtained.

The invention provides a method for processing fresh dendrobium devonianum stem powder. The method comprises the following steps: washing and peeling fresh dendrobium devonianum stems, cutting into sections, precooling, freeze-drying, crushing with liquid nitrogen, and drying at low temperature to prepare light yellow green dendrobium devonianum powder, wherein the fresh dendrobium devonianum stems are precooled by adopting a cryogenically quick-freeze method after being cut into sections to freeze the fresh dendrobium devonianum stems; the frozen dendrobium devonianum sections are put into a cold trap for vacuum freeze-drying and are ground into powder by virtue of a liquid nitrogen grinding machine, are dried in a thermostat at low temperature of 60-80 DEG C for 1-3 hours; and the dried powder is sealed and packaged.

The invention mainly discloses a Chinese yam high-yield and orientation cultivation method. According to Chinese yam growth characteristics and expansion growth characteristics of yam underground tubers, U-shaped groove supergene orientation cultivation is implemented, a series of improved cultivation techniques of early-stage preparation, timely planting, field management, timely harvesting and the like are implemented, seed nature degeneration is relieved, original high-yield biological natures of Chinese yams are furthest kept, the Chinese yams rapidly and strongly grow, planting density can be increased, the planting quantity per mu can reach 1500 plants, the yield per mu can reach 3000 kilograms, and the method has the advantages of high yield, less disease, simplicity and conveniencein harvesting and the like. By the method, holes cannot be deeply dug when the Chinese yams are planted, the Chinese yams are planted in pre-buried U-shaped supergene orientation grooves, the Chineseyams are harvested by the aid of simple tools, operation is simple, production cost is reduced, and the yield is high. According to the method, water is rapidly discharged, moisture can be preserved,planting and harvesting are facilitated, planting efficiency is improved, the nutrition quality of Chinese yam products is high, and planting economic benefits are remarkable.