47results about How to "Maintain biological characteristics" patented technology

The invention discloses an extraction method of filament eucommia rubber from eucommia leaves and peel; the method uses petrol ether as solvent to extract the eucommia rubber and has the steps: first, the eucommia leaves or the peel is made into small pieces and added with NaOH solution to dissolve cuticle; then cellulase is added to hydrolyze cell wall; the petroleum ether solvent at 60 DEG C to 90 DEG C of boiling range is added; then the obtained solution is extracted through recirculation at the temperature of 85 DEG C and filtrated when being hot, then cooled and frozen; the filament eucommia rubber is produced after filtration. Concentration of the NaOH solution is 0.5 percent to 1.5 percent and the dissolution time is 6 hours; pH value of the cellulase used for hydrolyzing the cell wall is 4 at temperature of 50 DEG C; enzymatic hydrolysis time is 1 hour to 2 hours and the time for reflux extraction is 2 hours; the frozen time is 30 minutes to 60 minutes at temperature of minus 20 DEG C to 0 DEG C. The method can effectively and completely extract the eucommia rubber to make original eucommia rubber and keep the original biological characteristics and physical properties and status and the original molecular structure and polymerization of the eucommia rubber; the method uses the cellulose to extract pretreated eucommia leaves raw materials, and gets the eucommia rubber of high quality when getting high yield.

The invention relates to the technical field of clinical medicines, and particularly relates to a cell preservation solution and a preparation method and applications thereof. The cell preservation solution comprises albumin, glucose, vitamin C and basic preservation liquid, wherein the basic preservation liquid is a mixture of compound electrolyte injection and compound amino acid injection. The cell preservation solution disclosed by the invention achieves a good maintaining effect on the viability and morphology of more than two kinds of seed cells, and can preserve the seed cells for a long time, and maintain the biological characteristics of the seed cells, thereby significantly improving the therapeutic effect of the seed cells.

A production process of eggshell packaging container, which adopts the steps of cleaning and sterilizing eggs, opening a hole at the end of an air chamber, removing an eggshell film at the end of the air chamber, suctioning the contents of the eggshell, rinsing and drying in the eggshell. and sterilization as well as filling of contents and sealing of openings. On the one hand, the prepared eggshell container can replace plastic packaging to a certain extent, reducing the use of plastic packaging, and on the other hand, it can be used as waste, and utilizes the own characteristics of eggshells, and has the advantage of multi-layer antibacterial protection.