51 results about "Dendrobium devonianum" patented technology

The invention discloses a strain for promoting germination and seedling formation of a Dendrobium devonianum seed and application thereof. The classification designation of the strain is Tulasnella sp.CY collected to China General Microbiological Culture Collection Center, wherein the collection number is CGMCC NO.9551. The strain can obviously promote germination and seedling formation of the Dendrobium devonianum seed, and has higher specificity. The strain has very import practical meanings for acquiring the symbiotic seed and seedling of Dendrobium devonianum through seed, can be used for nursery seedling raising or outdoor trunk seed direct sowing, has the advantages of low cost, high outdoor survival rate, no tissue degradation and the like as compared with tissue culture, has the application value of acquiring the symbiotic seed and seedling instead of tissue culture, and provides reliable technical guarantees for solving the bottleneck problem of seed and seedling sources in the Dendrobium devonianum culture industry.

The invention belongs to a health-care medicinal wine, and discloses a dendrobium devonianum flower wine and a preparation method thereof. According to a proportion, fresh dendrobium devonianum flowers and dried Chinese caterpillar fungus flowers are steeped into aged glutinous rice wine, the aged glutinous rice wine is then cellared for one to three years, and thereby the dendrobium devonianum flower wine is prepared. The wine assumes light amaranth, is vivid and bright, and has both the fragrance of the dendrobium devonianum flowers and the aroma of the glutinous rice wine; and the dendrobium devonianum flower wine is mellow, and has the effect of strengthening the body when being often drunk.

The invention provides instant dendrobe powder as well as a preparation method and an application thereof. The preparation method of the instant dendrobe powder adopts wet granulation twice and concretely comprises the following steps: mixing fresh dendrobe with water, pulping and homogenizing to obtain slurry; adding auxiliary materials into the slurry, and carrying out spray draying to obtain dendrobe powder; mixing the dendrobe powder with ethyl alcohol, preparing a soft material and then carrying out granulation to obtain dendrobe granules; and drying the dendrobe granules to obtain the instant dendrobe powder, wherein the dendrobe comprises dendrobium officinale, dendrobium fimbriatum, nobile dendrobium, dendrobium thysiflorum, dendrobium devonianum paxt and the like. The preparation method of the instant dendrobe powder is simple, the obtained instant dendrobe powder is high in content of active ingredients, can be easily dissolved and can be widely applied to the field of medicines or health foods, so that the instant dendrobe powder has a good market prospect.

The invention discloses a preparation method of freeze-dried instant powder containing dried tubers of dendrobium devonianum or dendrobium officinale kimura. The preparation method comprises the following technological processes of pretreating raw materials namely fresh tubers of the dendrobium devonianum or the dendrobium officinale kimura, performing freezing, performing freeze drying, performing crushing, performing blending, performing mixing, performing pelletization, performing subpackaging, performing packaging, obtaining finished products and the like. The processed freeze-dried products are good in rehydration performance, high in dissolving speed, convenient to carry and drink, and long in quality guarantee period, and have a broad market prospect.

The invention discloses a dendrobium devonianum planting fertilizing method which comprises the following steps of first, fertilizing period selecting; second, fertilizing frequency selecting; third, fertilizing manure selecting; and fourth, fertilizing concentration control. In the third step, selected fertilizing manure needs to be rich in nitrogen, phosphorus and potassium and comprises microelements of calcium, ferrum, zinc, sulphur, boron, manganese, molybdenum and copper. In a planting process, main nutrient elements rich in nitrogen, phosphorus and potassium and microelements such as calcium, ferrum, zinc, sulphur, boron, manganese, molybdenum and copper are applied, matching is reasonable, the phenomenon that in the prior art, improper fertilizing raw materials are used, so that dendrobe rooting and bad growing happen is avoided, the whole technical scheme is easy to carry out, and promotion and application can be achieved.

The invention discloses a method for improving the survival rate of transplanted test tube dendrobium devonianum seedlings by resistance processing, which comprises the steps of early-stage cultivation, resistance processing, late-stage cultivation and seedling transplant and has the advantages that: the plants are placed under an extreme temperature condition to reduce the growth and metabolism speed of the plants to promote the fibration of stems and strengthen the environmental adaptability of the plants; and in an artificial adverse environment, the stems and twigs of the plants expand quickly, leaves fall and resistance increases, and thus the transplant survival rate is improved. The method is a simple resistance processing process with high maneuverability and low cost.

The invention discloses a method for separating and purifying dendrobium devonianum homogeneous polysaccharides and aims to solve the problem that the separation, purification and structural analysis of dendrobium devonianum polysaccharides have certain difficulty due to the fact that the dendrobium devonianum polysaccharides are high in viscosity and large in high-activity polysaccharide segment molecular weight. The method includes: separating with a step-by-step ethanol precipitation method, and purifying with a gel column to obtain pure the dendrobium devonianum homogeneous polysaccharides. In addition, the invention further uses multispectral analysis to identify the skeletal structure of the dendrobium devonianum homogeneous polysaccharides, the primary structure of the dendrobium devonianum homogeneous polysaccharides can be identified accurately, and the main polysaccharides in dendrobium devonianum can be determined as acetyl glucomannan.

The invention discloses a quality detection method for Dendrobium devonianum Paxton. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety of a to-be-detected Dendrobium devonianum Paxton herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and/ornaringenin as a reference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium devonianum Paxton; and 4) establishing an analysis model by using the content value of the schaftoside and/or naringenin in the chromatographic data as the response variable and using the peak area value of other components in the fingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component. According to the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium devonianum Paxton is precisely identified and controlled.

The invention relates to a dendrobium-devonianum-paxt-dried-stem polysaccharide with the effects of immunological enhancement and the hypoglycemic activity and a preparing method. The preparing method of the dendrobium-devonianum-paxt-dried-stem polysaccharide includes the steps of water extraction, alcohol precipitation, redissolving, drying or water extraction, ultra-filtration, drying and the like; the dendrobium-devonianum-paxt-dried-stem polysaccharide is characterized in that development does not occur when an iodine-potassium iodide reagent is added, the molecular weight is 3*10<5> Da-5*10<5> Da, a monosaccharide composition mainly comprises mannose, Acetyl-Man an a small amount of glucose (Glc), and the composition ratio of the mannose to the Glc is larger than 20:1; a glycosidic bond is mainly beta-1,4-Manp and beta-1,4-Glcp. The dendrobium-devonianum-paxt-dried-stem polysaccharide has the effects of immunological enhancement and the hypoglycemic activity, and can be used for developing relative products for treating immunosuppression and hyperglycemia.

The invention discloses a method for processing golden dendrobium devonianum bars. The method comprises the steps of (1) pretreatment, (2) microwave oven baking and (3) insolation under the sun. The pretreatment comprises classification, tunica vaginalis removal and stem subsection. According to the stem subsection, a stem is cut into stem sections at joints of the stem; according to the microwave oven baking, baking treatment is carried out intermittently six times, the stem sections are taken out to be stirred evenly after being baked every time, and the stem sections are placed in a microwave oven to be baked again after moisture is volatilized. According to the insolation under the sun, all the stem sections of dendrobium devonianum are hardened, and the stem sections become golden. The stem sections can be continuously insolated until the dry-wet ratio of the golden dendrobium devonianum bars is 1:6, and products are obtained. Infrared baking can also be adopted, product obtaining is accelerated, the temperature is controlled to be 100 DEG C, baking is carried out until the dry-wet ratio of the golden dendrobium devonianum bars is 1:6, and the products are obtained. The processed products are golden and in a bar shape, the quality the same as that of dendrobium candidum is kept, the operation is easy and convenient, the method is easy to master and suitable for industrialized production, processing time is shortened, and production cost is greatly lowered.

Ganoderma lucidum tea with Dendrobium and a production process thereof are provided. The Ganoderma lucidum tea with Dendrobium comprises 10-12 parts of Dendrobium devonianum, 40-50 parts of Ganoderma lucidum, 25-30 parts of brown rice tea, and 15-20 parts of orange seed. The Ganoderma lucidum tea with Dendrobium can retain original sweet and bitter tastes of Ganoderma lucidum and natural fragrance of Dendrobium devonianum, can eliminate evil to nourish yin and tonify without converging evil and has the unique effect of supplementing with light dishes to maintain health; in addition, the Ganoderma lucidum tea with Dendrobium has delicate fragrance of brown rice tea, fragrance of rice and faint orange sour of orange seeds, has unique taste, and has refreshing effect and skin nourishing effect; the Ganoderma lucidum tea with Dendrobium can be consumed often to nourish yin and clear heat, improve sleep, lower blood pressure, adjust blood lipid, lower blood glucose and the like. Besides these effects, as Dendrobium devonianum has low price, the Ganoderma lucidum tea with Dendrobium prepared herein has significantly lower cost than that of traditional Ganoderma lucidum tea with Dendrobium; therefore, market acceptance of the Ganoderma lucidum tea with Dendrobium can be increased greatly, the competitiveness of the Ganoderma lucidum tea with Dendrobium is improved, and the Ganoderma lucidum tea with Dendrobium may earn a place in the tea market that is gradually saturated nowadays.

The invention provides dendrobium devonianum rapid propagation seedling survival culture medium series and a tissue culture method. The dendrobium devonianum rapid propagation seedling survival culture medium series comprise a sowing culture medium, a multiplying culture medium, a seedling strengthening culture medium, a differentiated seedling culture medium and a rooting culture medium. The tissue culture method comprises the steps that seeds are cultivated in a sprouting culture medium firstly, light treatment is directly conducted without darkness cultivation treatment, after the seeds spout and form protocorms, the protocorms are subjected to induction in a protocorms multiplying inducing culture medium to produce callus tissues, and the callus tissues further form protocorm-like bodies; the protocorm-like bodies are subjected to differentiation to form young seedlings; the young seedlings are transplanted into the seedling strengthening culture medium, after the young seedlings grow up to be big seedlings, the big seedlings are cultivated in the rooting culture medium to be dendrobium devonianum mature seedlings. By means of the dendrobium devonianum rapid propagation seedling survival culture medium series and the tissue culture method, the cultivating time of dendrobium devonianum can be shortened, not only is the operation simple, but also the method is not limited by the time and the place, and the continuous division growth of the protocorm-like bodies can guarantee anniversary production, and the large-scale industrial production and application are facilitated.

The invention discloses a preparation method of herba dendrobium devonianum paste. The preparation method comprises the following steps: (1) selecting fresh herba dendrobium devonianum, washing the fresh herba dendrobium devonianum to be clean, peeling the fresh herba dendrobium devonianum, and cutting the peeled fresh herba dendrobium devonianum into segments; (2) selecting high-quality fructus ziziphi jujubae, washing the high-quality fructus ziziphi jujubae to be clean, and removing the stones of the washed high-quality fructus ziziphi jujubae; (3) adding the fresh herba dendrobium devonianum and fructus ziziphi jujubae into a juicer at the weight ratio of 2 to 1, and adding 25-35-fold amount of water into the juicer; (4) carrying out juicing for 1-3 min; (5) carrying out filtering; (6) heating the filtered liquid under moderate heat till the filtered liquid reaches the boiling state; (7) boiling the liquid under gentle heat for 15-25 min, thus concentrating the liquid to form paste; and (8) placing the paste till the paste reaches the cool state, packaging the paste into a bottle, and carrying out sealed storage. According to the preparation method, the juicing method is adopted, herba dendrobium devonianum and fructus ziziphi jujubae are juiced after being mixed, so that the palatability of the herba dendrobium devonianum paste is increased, meanwhile, the preparation time and cost of the herba dendrobium devonianum paste are also reduced, after the two medicine-food homologous materials, namely, herba dendrobium devonianum and fructus ziziphi jujubae are adopted for preparing the paste, the obvious effect of enhancing the immunity is achieved.

The invention discloses an artificial breeding method for symbiotic germchit of Dendrobium devonianum. The method comprises the steps of subjecting Tulasnella sp., of which the collection number is CGMCC No. 9551, to amplified fermenting culture, filtering mycelia, adding a proper volume of sterile water into the mycelia, carrying out crushing by a refiner so as to prepare a fungus suspension, adding Dendrobium devonianum seeds into the fungus suspension so as to prepare a seed-fungus suspension, preparing a seedling raising matrix from coco coir, tree leaves and tree barks, sprinkling 50-500ml of the seed-fungus suspension to the matrix per square meter, and carrying out breeding for 45 days while maintaining the moisture content of the seedling raising matrix to 50-90%, thereby obtaining a large number of symbiotic young seedlings. According to the method, the seedling forming time of the seeds can be remarkably shortened, the seedling raising cost can be reduced, a relatively large number of Dendrobium devonianum young seedlings can be obtained in one time, and the growth speed and natural-environment-transplanted survival rate of the symbiotic young seedlings are improved and are higher than those of asymbiotic-germinated young seedlings. The method achieves a new way for obtaining the seed seedlings from the Dendrobium devonianum seeds and has important practical popularization and application values.

The invention discloses a dendrobium devonianum clone seedling reproduction method and relates to the technical field of bioengineering. In the technical scheme of the reproduction method, a high-quality single dendrobium devonianum plant is used as raw material; a stalk section is used as an explant; induction culture, propagation culture and rooting culture are carried out after disinfection; and in every culture phase, temperature is controlled to be 25+/-2 DEG C; illumination intensity is controlled to be 1500-2000 lx; illumination time is 12h/day; and environmental noise is less than 40db. The method not only enables a seedling to maintain excellent shape and properties of a parent plant, but also improves seedling reproduction coefficient, thereby laying a foundation for industrialized production of dendrobium devonianum.

The invention discloses a method for identifying Dendrobium aphyllum (Roxb.)C. E. Fisch., Dendrobium officinale Kimura et Migo and Dendrobium devonianum Paxt. The method comprises the following steps of: firstly, sample processing, secondly, spectrum acquisition, thirdly, spectral data preprocessing, fourthly, establishing an identifying model, and fifthly, predicting and identifying the Dendrobium aphyllum (Roxb.)C. E. Fisch., Dendrobium officinale Kimura et Migo and Dendrobium devonianum Paxt. on the market. The method is a simple and effective method for identifying the Dendrobium aphyllum (Roxb.)C. E. Fisch., Dendrobium officinale Kimura et Migo and Dendrobium devonianum Paxt., and the identification accuracy rate reaches up to 100 percent.

The invention provides a method for processing fresh dendrobium devonianum stem powder. The method comprises the following steps: washing and peeling fresh dendrobium devonianum stems, cutting into sections, precooling, freeze-drying, crushing with liquid nitrogen, and drying at low temperature to prepare light yellow green dendrobium devonianum powder, wherein the fresh dendrobium devonianum stems are precooled by adopting a cryogenically quick-freeze method after being cut into sections to freeze the fresh dendrobium devonianum stems; the frozen dendrobium devonianum sections are put into a cold trap for vacuum freeze-drying and are ground into powder by virtue of a liquid nitrogen grinding machine, are dried in a thermostat at low temperature of 60-80 DEG C for 1-3 hours; and the dried powder is sealed and packaged.

The invention discloses a Dendrobium devonianum Paxt fermented beverage with a health-care function and a preparation method of the Dendrobium devonianum Paxt fermented beverage, and belongs to the technical field of beverage preparation. The Dendrobium devonianum Paxt residues are used as raw materials, and the finished beverage is prepared through preparation, fermentation and filtration. The invention aims to provide the Dendrobium devonianum Paxt fermented beverage which takes Dendrobium devonianum Paxt residues as raw materials and has a stomach-thickening health-care function and the preparation method thereof.

The invention discloses a method for building a relevant characteristic fingerprint spectrum of dendrobium devonianum chemical micromolecular compositions, the method comprises the following steps that (1) samples with the sample sizes of n are subjected to chromatographic detection respectively, and the chemical micromolecular composition schaftoside and/or naringenin are/is taken as detection data of reference ingredients; (2) the samples are subjected to fingerprint spectrum detection, and the fingerprint spectrum peak area value of the whole chemical composition of dendrobium devonianum isobtained; (3) the composition content of the schaftoside and/or naringenin in the spectrum data are/is taken as response variables, the peak area values of other compositions in the fingerprint spectrum are taken as independent variables to build an analysis model, and a micromolecular composition and fingerprint spectrum linear regression model is built; (4) the relevant characteristic fingerprint spectrum of the micromolecular compositions is built for the contribution degree of the regression model through the variable correlation coefficient index representation independent variables. According to the method for building the relevant characteristic fingerprint spectrum of the dendrobium devonianum chemical micromolecular compositions, the correlation analysis of the chemical micromolecular composition contents is realized, and the quality of the dendrobium devonianum medical material can be effectively identified, controlled and evaluated.

The invention provides dendrobium nobile and ganoderma lucidum tea with health care effects and a production process thereof. The dendrobium nobile and ganoderma lucidum tea is prepared from 10-12 parts of dendrobium devonianum paxt, 45-55 parts of ganoderma lucidum, 20-30 parts of buckwheat tea and 20-25 parts of orange seeds. The prepared dendrobium nobile and ganoderma lucidum tea can preserve the bitter and sweet primary taste of the ganoderma lucidum and the natural dendrobium fragrance of the dendrobium devonianum paxt, has the effects of eliminating the pathogenic factor, nourishing yin and conducting tonifying without converging evil, has the secret of moistening tonification and preserving health, and also has various health care and health preserving effects of the buckwheat tea and the orange seeds. The dendrobium nobile and ganoderma lucidum tea also has the effects of nourishing yin, resolving heat, improving sleep, reducing blood pressure, regulating blood fat, reducing blood sugar, clearing throat, protecting liver, improving eyesight, beautifying face, delaying aging and preventing cancer by long-term drinking. Other than the effects, owing to low price of the dendrobium devonianum paxt, the cost of the prepared dendrobium nobile and ganoderma lucidum tea is obviously reduced in comparison to the conventional dendrobium nobile and ganoderma lucidum tea, so the market acceptability of the dendrobium nobile and ganoderma lucidum tea can be greatly improved, the product competitive power is improved, and the dendrobium nobile and ganoderma lucidum tea can also have a share in the present gradually saturated tea market.

The invention discloses a novel organic barley powder compound beverage and a preparation process thereof. The beverage is prepared from the following raw materials in percentage by weight: 40-50% ofgrapefruit juice, 15-25% of barley leaf powder juice, 15-25% of dendrobium devonianum flower juice, 10-15% of water and carbon dioxide of which the volume is 6-10 times of that of the juice. The preparation method comprises the following steps of preparing the grapefruit juice, preparing the barley leaf powder juice, preparing the dendrobium devonianum flower juice, performing blending, performingcooling, adding gas, performing canning and performing sterilizing. According to the preparation process, a small amount of the carbon dioxide is uniformly added into the juice, so that the beveragehas refreshing and pleasant mouth feel, the preservative and sterilization effects can be achieved, and the juice has the characteristics of long shelf life and difficulty in production of microorganisms. The beverage is free from additives, rich in polyphenols, rich in nutrients and unique in mouth feel. The preparation process is simple, industrial production is facilitated, the additional valueof agricultural products of grapefruits and dendrobium devonianum can be increased, and development and utilization of new barley leaf products are expanded.