Anti-inflammatory and anti-aging composition containing crocus sativus extract and application of anti-inflammatory and anti-aging composition

Abstract

The invention discloses an anti-inflammatory and anti-aging composition containing a crocus sativus extract and application of the anti-inflammatory and anti-aging composition, and relates to the technical field of cosmetics, the anti-inflammatory and anti-aging composition comprises the crocus sativus extract, a kangaroo claw extract, a dendrobium devonianum stem extract and adenosine; the anti-inflammatory and anti-aging composition can promote human dermal fibroblasts to secrete III-type collagen, can promote human dermal fibroblasts to proliferate and improve the anti-oxidation and anti-inflammatory capacity of the human dermal fibroblasts, has certain anti-inflammatory and anti-aging effects, and can be widely applied to cosmetics with the anti-inflammatory and anti-aging effects.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to an anti-inflammatory and anti-aging composition containing saffron extract and application thereof. BACKGROUND

[0002] With the in-depth study of skin health and aging mechanism, anti-inflammatory and anti-aging has become the core direction of cosmetic research. Skin aging is a complex biological process involving intrinsic physiological aging and multi-dimensional damage such as oxidative stress, chronic inflammation and extracellular matrix (ECM) degradation induced by external environmental factors (such as ultraviolet radiation, pollutants). Studies have shown that there is a close relationship between inflammation and aging, and chronic inflammation can accelerate the skin aging process through pathways such as activation of matrix metalloproteinases (MMPs) and inhibition of collagen synthesis, forming a vicious cycle of "inflammation-aging". The anti-inflammatory and anti-aging cosmetics on the market mainly have the following technical bottlenecks:

[0003] Single component limitation: Most products rely on a single active ingredient (such as vitamin C, hyaluronic acid), which has antioxidant or moisturizing functions, but it is difficult to achieve multi-dimensional regulation of anti-inflammatory, collagen synthesis and barrier repair;

[0004] Insufficient synergistic effect: Lack of scientific compatibility between components, leading to scattered efficacy, and even reduced bioavailability due to conflicting physicochemical properties;

[0005] Risk of irritation: Some high-efficiency components (such as high-concentration retinol) are prone to cause skin sensitivity, making it difficult to meet the needs of sensitive skin groups;

[0006] Single target: Existing solutions focus on ECM reconstruction or free radical scavenging, ignoring the regulation of cellular energy metabolism and the repair of microecological balance.

[0007] Studies have shown that dermal fibroblasts, as the core cells of dermal matrix synthesis, their functional decline (such as reduced secretion of type III collagen and decreased antioxidant enzyme activity) is an important marker of skin aging. At the same time, overactivation of the NF-κB pathway under chronic inflammatory conditions can exacerbate collagen degradation and cell apoptosis. Therefore, there is an urgent need to develop a new composition that can systematically regulate the inflammation-aging axis and enhance the cell's own repair ability. SUMMARY

[0008] The purpose of the present application is to overcome the deficiencies of the prior art and provide an anti-inflammatory and anti-aging composition containing saffron extract which can effectively resist inflammation, aging, improve skin elasticity, and improve the antioxidant and anti-inflammatory ability of human dermal fibroblasts without irritation.

[0009] To achieve the above purpose, the technical scheme adopted by the present application is as follows:

[0010] The first aspect: the present application provides an anti-inflammatory and anti-aging composition, the composition comprises the following components by mass fraction:

[0011] Saffron extract: 0.2-0.7 parts;

[0012] Banksia extract: 0.2-0.5 parts;

[0013] Dendrobium stem extract: 0.2-1 parts;

[0014] Adenosine: 1-5 parts.

[0015] The saffron extract, banksia extract and dendrobium stem extract are all water-soluble.

[0016] Further, the composition comprises the following components by mass fraction:

[0017] Saffron extract: 0.3-0.6 parts;

[0018] Banksia extract: 0.3-0.4 parts;

[0019] Dendrobium stem extract: 0.5-0.8 parts;

[0020] Adenosine: 2-4 parts.

[0021] Further, the composition comprises the following components by mass fraction;

[0022] Saffron extract: 0.5 parts;

[0023] Banksia extract: 0.4 parts;

[0024] Dendrobium stem extract: 0.7 parts;

[0025] Adenosine: 3 parts;

[0026] Wherein:

[0027] The saffron extract has antioxidant and anti-inflammatory effects, can scavenge free radicals and block oxidative stress, inhibit peroxidation, and inhibit the release of inflammatory factors to reduce the damage of chronic inflammation to the skin.

[0028] The banksia extract can stimulate collagen synthesis and expression of tendon protein X to enhance skin elasticity.

[0029] The dendrobium stem extract can activate endogenous antioxidant enzymes, repair photoaging damage, regulate aquaporin, increase the water content of the stratum corneum, promote the balance of epidermal microecology, and repair the skin barrier.

[0030] Adenosine can improve mitochondrial function, alleviate epidermal atrophy, inhibit MMP-1 activity, reduce collagen fiber breakage, delay skin relaxation, promote keratinocyte differentiation, and enhance the skin's self-repairing ability.

[0031] The present study found that the combination of the above components has synergistic effects and can achieve excellent anti-inflammatory, anti-aging, skin elasticity improvement, and skin cell anti-inflammatory and anti-aging capabilities, and is mild and non-irritating.

[0032] In a second aspect, the present application provides a serum with anti-inflammatory and anti-aging effects, which comprises the anti-inflammatory and anti-aging composition of the first aspect.

[0033] Further, the serum with anti-inflammatory and anti-aging effects further comprises a solvent, a pH adjusting agent, and a preservative.

[0034] Further, the solvent is deionized water; the pH adjusting agent is at least one of arginine and disodium hydrogen phosphate; and the preservative is at least one of p-hydroxyacetophenone, 1,2-hexanediol, and 1,2-pentanediol.

[0035] Compared with the prior art, the present application has the following advantages:

[0036] The present application provides an anti-inflammatory and anti-aging composition, in which the four raw materials can cooperate with each other and have synergistic effects to improve skin elasticity, enhance the anti-inflammatory and anti-aging capabilities of skin cells, and are overall mild and non-irritating.

[0037] Specifically, the present application innovatively selects and uses saffron extract, kangaroo paw extract, dendrobium stem extract, and adenosine in specific mass fractions to obtain an anti-inflammatory and anti-aging composition, in which:

[0038] Crocins in saffron extract block oxidative stress by directly scavenging free radicals, while polysaccharide components in dendrobium stem extract activate endogenous antioxidant enzyme systems, both of which synergistically inhibit lipid peroxidation and release of inflammatory factors, significantly reducing collagen degradation caused by photoaging and chronic inflammation.

[0039] Kangaroo paw extract stimulates the synthesis of type I collagen and tenascin X to improve skin elasticity; dendrobium stem extract regulates aquaporin AQP3 through polysaccharide components to enhance the water content of the stratum corneum, which is complementary to the ATP energy supply of adenosine, promotes the synthesis of ceramides, and realizes the dual repair of barrier function and epidermal microecology.

[0040] Adenosine improves cell energy metabolism by activating A2A receptors, relieves epidermal atrophy caused by mitochondrial dysfunction, and inhibits MMP-1 activity to reduce collagen fiber rupture. The combination of the above three effects forms an "energy-structure-function" closed loop with the antioxidant and collagen-promoting effects, thereby delaying the formation of wrinkles and the process of skin relaxation.

[0041] The synergistic effect of the four components significantly improves the anti-aging and anti-inflammatory effects compared to single components, and there is no toxicity of component stacking, which is suitable for the development of high-performance skincare products. DETAILED DESCRIPTION

[0042] In order to better understand the present application, the following further describes the present application in conjunction with specific examples, wherein the "composition" and "application examples" used are for the purpose of describing specific embodiments and do not constitute a limitation on the scope of protection of the present application.

[0043] The experimental methods in the following examples not specified in the specific conditions are usually carried out according to the conventional conditions or according to the conditions recommended by the manufacturer. Unless otherwise specified, the percentages and mass parts are calculated.

[0044] Some raw materials and sources are as follows:

[0045] Saffron extract was purchased from Yunnan Inge Biotechnology Co., Ltd.; trade name: Zangyun TM ;

[0046] Kangaroo paw extract was purchased from Guangzhou Yihewo Trade Co., Ltd.; trade name: SKINectura TM ;

[0047] Dendrobium stem extract was purchased from Yunnan Inge Biotechnology Co., Ltd.; trade name: Dendrobium stem extract

[0048] Adenosine was purchased from Guangdong Dingchun Biomedicine Technology Co., Ltd.; trade name: Adenosine

[0049] HaCaT cells, human dermal fibroblasts, HaCaT cell special medium, and human dermal fibroblast complete medium were purchased from Beina Biological Technology Co., Ltd.

[0050] The remaining raw materials are commercially available.

[0051] The raw material composition and preparation method of the anti-inflammatory and anti-aging composition are as follows:

[0052] Composition 1:

[0053] Saffron extract: 0.5 parts

[0054] Kangaroo paw extract: 0.4 parts

[0055] Dendrobium stem extract: 0.7 parts

[0056] Adenosine: 3 parts

[0057] Preparation method: take the above mass parts of raw materials, mix evenly at 25°C, seal and store away from light.

[0058] Composition 2:

[0059] Crocosia extract: 0.2 parts;

[0060] Banksia extract: 0.2 parts;

[0061] Dendrobium stem extract: 0.2 parts;

[0062] Adenosine 1 part;

[0063] Preparation method same as composition 1.

[0064] Composition 3:

[0065] Crocosia extract: 0.7 parts;

[0066] Banksia extract: 0.5 parts

[0067] Dendrobium stem extract: 1 part

[0068] Adenosine: 5 parts

[0069] Preparation method same as composition 1.

[0070] Composition ①: different from composition 1, lack of Crocosia extract, lack of mass parts of Banksia extract, Dendrobium stem extract and Adenosine with mass ratio of 0.4:0.7:3, and its preparation method is the same as composition 1.

[0071] Composition ②: different from composition 1, lack of Banksia extract, lack of mass parts of Crocosia extract, Dendrobium stem extract and Adenosine with mass ratio of 0.5:0.7:3, and its preparation method is the same as composition 1.

[0072] Composition ③: different from composition 1, lack of Dendrobium stem extract, lack of mass parts of Crocosia extract, Banksia extract and Adenosine with mass ratio of 0.5:0.4:3, and its preparation method is the same as composition 1.

[0073] Composition ④: different from composition 1, lack of Adenosine, lack of mass parts of Crocosia extract, Banksia extract and Dendrobium stem extract with mass ratio of 0.5:0.4:0.7, and its preparation method is the same as composition 1.

[0074] Composition ⑤:

[0075] Different from composition 1, the Dendrobium stem extract is replaced by Dendrobium candidum Wall ex Lindl stem extract with the same quality, and the rest of the raw materials and preparation method are the same as composition 1.

[0076] Composition ⑥:

[0077] Crocus sativus extract: 3 parts

[0078] Acacia extract: 0.7 parts

[0079] Dendrobium stem extract: 0.1 parts

[0080] Adenosine: 0.8 parts

[0081] The preparation method is the same as composition 1.

[0082] A raw material composition of an essence with anti-inflammatory and anti-aging effects and its preparation method are as follows:

[0083] Essence 1:

[0084] Composition 1: 3wt%;

[0085] Preservative: 0.5wt%;

[0086] pH regulator: 0.1wt%:

[0087] Solvent: supplement to 100wt%;

[0088] Preparation method: mix the composition with solvent, preservative and pH regulator uniformly at room temperature, and then fill it in;

[0089] The preservative is p-hydroxyacetophenone, the pH regulator is arginine, and the solvent is deionized water.

[0090] Essence 2:

[0091] Different from essence 1, composition 2 is used to replace composition 1, and the rest of the raw materials and preparation method are the same as essence 1.

[0092] Essence 3:

[0093] Different from essence 1, composition 3 is used to replace composition 1, and the rest of the raw materials and preparation method are the same as essence 1.

[0094] Essence ①:

[0095] Different from essence 1, composition ① is used to replace composition 1, and the rest of the raw materials and preparation method are the same as essence 1.

[0096] Essence ②:

[0097] Different from essence 1, composition ② is used to replace composition 1, and the rest of the raw materials and preparation method are the same as essence 1.

[0098] Serum 3:

[0099] The difference from serum 1 is that composition 3 is used to replace composition 1, and the rest of the raw materials and preparation method are the same as serum 1.

[0100] Serum 4:

[0101] The difference from serum 1 is that composition 4 is used to replace composition 1, and the rest of the raw materials and preparation method are the same as serum 1.

[0102] Serum 5:

[0103] The difference from serum 1 is that composition 5 is used to replace composition 1, and the rest of the raw materials and preparation method are the same as serum 1.

[0104] Serum 6:

[0105] The difference from serum 1 is that composition 6 is used to replace composition 1, and the rest of the raw materials and preparation method are the same as serum 1.

[0106] Serum 7:

[0107] Preservative: 0.5wt%;

[0108] pH adjuster: 0.1wt%:

[0109] Solvent: supplement to 100wt%;

[0110] Preparation method: mix the solvent, preservative, and pH adjuster uniformly at room temperature, and then fill.

[0111] The preservative is p-hydroxyphenylacetone, the pH adjuster is arginine, and the solvent is deionized water.

[0112] Efficacy test:

[0113] Test 1: Inflammatory factor inhibition test of composition

[0114] Test sample: composition 1-3, composition ①-⑥.

[0115] Test sample preparation: use HaCaT cell culture medium to prepare the test sample with a concentration of 1mg / mL.

[0116] Test cell: HaCaT cell.

[0117] Test medium: Hacat cell special medium.

[0118] Culture HaCaT cells in a 6-well plate, 1x10^5 cells per well. Use the test sample to treat the cells for 24 hours.

[0119] After treatment, the cells were stimulated by adding LPS (1 μg / mL) and incubated for 24 hours to induce inflammatory response.

[0120] Total RNA was extracted from the cells and reverse-transcribed into cDNA. The gene expression of inflammatory factor IL-6 was detected by qPCR, and the relative expression of the gene was analyzed by the method of ΔCt. The calculation method is as follows:

[0121] Relative expression of inflammatory factor (%) = Inflammatory factor level of treatment group / Inflammatory factor level of LPS group * 100%

[0122] The test results are shown in Table 1.

[0123] Table 1

[0124]

[0125]

[0126] Based on the results in Table 1, it can be seen that compositions 1-3 and compositions ①-⑥ can effectively reduce the relative expression of inflammatory factors of HaCaT cells. By comparing the results of composition 1 and compositions ①-④, it can be seen that the crocus sativus extract, the kangaroo paw extract, the dendrobium stem extract, and the adenosine in the composition defined in the present application have a certain synergistic effect, and the use of the three together can reduce the relative expression of inflammatory factors of HaCaT cells. By comparing the results of composition 1 and composition ⑤, it can be seen that compared with the dendrobium stem extract, the synergistic effect between the dendrobium stem extract, the crocus sativus extract, the kangaroo paw extract, and the adenosine is better. By comparing the results of composition 1 and composition ⑥, it can be seen that the crocus sativus extract, the kangaroo paw extract, the dendrobium stem extract, and the adenosine compounded in the mass ratio defined in the present application have a better effect on reducing the relative expression of inflammatory factors of HaCaT cells.

[0127] Test 2: Effect of the composition on the proliferation ability of human dermal fibroblasts (HDmF)

[0128] Test sample: compositions 1-3, compositions ①-⑥.

[0129] Test sample preparation: the composition is prepared into a 1 mg / mL concentration mixture using the test medium, and it is prepared as needed.

[0130] Test cell: human dermal fibroblasts (HDmF).

[0131] Test medium: human dermal fibroblast complete culture medium.

[0132] MTT colorimetric method: human dermal fibroblasts were cultured in advance in the test medium at 37℃ in a 5% CO2 incubator. At the time of the experiment, the logarithmic growth phase human dermal fibroblasts were digested with trypsin and inoculated into a 96-well plate at a density of 5000 cells per well, with a volume of 200 μL per well. After 24 h in the incubator, the old culture medium was carefully aspirated after the cells adhered. 100 μL of the test sample was added to each well, with 5 replicates per group. A control group was also set up, with only 100 μL of the test medium added. After 48 h of continuous culture, 20 μL of MTT solution (5 mg / mL, prepared with PBS buffer) was added to each well, and incubated at 37℃ in the dark for 4 h. After incubation, the supernatant was carefully aspirated, 150 μL of DMSO was added to each well, and the crystalline material was fully dissolved by shaking at low speed on a shaker for 10 min. Finally, the absorbance value (OD value) at 490 nm was measured on a microplate reader. Cell proliferation rate = (experimental group OD value - control group OD value) / control group OD value x 100%. The detection results were averaged, as shown in Table 2 below.

[0133] Table 2

[0134]

[0135]

[0136] Based on the results in Table 2, compositions 1-3 and compositions ①-⑥ can effectively improve the proliferation rate of human dermal fibroblasts. Comparing the results of composition 1 and compositions ①-④, it can be seen that the crocus sativa extract, kangaroo paw extract, dendrobium stem extract, and adenosine in the composition defined in the present application have a certain synergistic effect, and their joint use can promote the proliferation rate of human dermal fibroblasts. Comparing the results of composition 1 and composition ⑤, it can be seen that the synergistic effect between the dendrobium stem extract and the crocus sativa extract, kangaroo paw extract, and adenosine is better than that of the dendrobium stem extract. Comparing the results of composition 1 and composition ⑥, it can be seen that the crocus sativa extract, kangaroo paw extract, dendrobium stem extract, and adenosine compounded at the mass ratio defined in the present application have the best effect on improving the proliferation rate of human dermal fibroblasts.

[0137] Test 3: Effect of the composition on the expression of collagen type III gene secreted by human dermal fibroblasts

[0138] Test sample: compositions 1-3, compositions ①-⑥.

[0139] Test sample preparation: the composition was prepared into a 1 mg / mL concentration mixture using the test medium, and was prepared as needed.

[0140] Test cell: human dermal fibroblasts (HDmF).

[0141] Test medium: human dermal fibroblast complete culture medium.

[0142] Fibroblasts were subcultured in the test medium in advance at 37℃ in a 5% CO2 incubator. During the experiment, logarithmically growing fibroblasts were digested with trypsin and inoculated into a 96-well plate at a density of 5000 cells / well in a volume of 200 μL per well. After 24 h in the incubator, the old culture medium was carefully aspirated after the cells adhered. The culture wells were divided into a test group, a blank control group and a model control group, wherein 150 μL of a H2O2 solution (150 μM, the test medium as a solvent) was used to treat the test group and the model control group for 1.5 h, and the blank control group was added with an equal amount of the test medium. After the treatment was completed, the liquid in each well was discarded, and then 150 μL of the test sample was added to the test group, and an equal amount of the test medium was added to the blank control group and the model control group. After 24 h of culture at 37℃ in a 5% CO2 incubator, the supernatant was discarded, and the cells were washed twice with PBS. The cells were collected, and the RNA was extracted. After reverse transcription into cDNA, RT-qPCR was performed to detect the relative expression of collagen type III gene (the expression of collagen type III gene in the blank control group was taken as 100%).

[0143] Table 3

[0144]

[0145]

[0146] Based on the results in Table 3, it can be known that, compared with the model control group and compositions 1-3 and compositions ①-⑥, the compositions can effectively improve the relative expression of collagen type III gene. Compared with the results of composition 1 and compositions ①-④, it can be known that the crocus sativus extract, the kangaroo paw extract, the dendrobium stem extract and the adenosine in the composition defined in the application have a certain synergistic effect, and the use of the three together can improve the relative expression of collagen type III gene. Compared with the results of composition 1 and composition ⑤, it can be known that, compared with the dendrobium stem extract, the synergistic effect of the dendrobium stem extract, the crocus sativus extract, the kangaroo paw extract and the adenosine is better. Compared with the results of composition 1 and composition ⑥, it can be known that, when the crocus sativus extract, the kangaroo paw extract, the dendrobium stem extract and the adenosine are compounded in the mass ratio defined in the application, the composition obtained has the best effect on improving the relative expression of collagen type III gene.

[0147] Test 4: Effect of the composition on the antioxidant capacity of human dermal fibroblasts

[0148] Test sample: compositions 1-3, compositions ①-⑥.

[0149] Test sample preparation: the composition is prepared into a 1 mg / mL concentration mixture solution using the test medium, and prepared immediately before use.

[0150] Test cells: human dermal fibroblasts (HDmF).

[0151] Test medium: human dermal fibroblast complete medium.

[0152] The fibroblasts are subcultured in the test medium in advance in a 37℃, 5% CO2 incubator. During the experiment, the logarithmically growing fibroblasts are digested with trypsin and inoculated into a 96-well plate at a density of 5000 cells / well in a volume of 200 μL per well. After 24 h in the incubator, the old medium is carefully aspirated after the cells are attached. 150 μL of the test sample is added to each well of the test group, and the same amount of test medium is added to the blank group and the model group. After 1 h of culture, an appropriate amount of H2O2 is added to the test group and the model group to make the H2O2 concentration in the wells 800 μMOL / L, and the same amount of test medium is added to the blank group. After 24 h of culture, the liquid in the wells is discarded, and the wells are washed 3 times with the test medium for 4 min each time. After washing, 100 μL of the fluorescent reagent DCFH-DA (10 μmol / L) is added to each well, and the cells are shaken to ensure full contact. The mixture is incubated in a 37℃, 5% CO2 incubator for 20 min in the dark. Then, the liquid in the wells is discarded, and the wells are washed 3 times with PBS for 4 min each time. After washing, the liquid in the wells is discarded, and the fluorescence of the cells is observed under a fluorescence inverted microscope. The fluorescence staining result of the blank group is taken as 100%, and the fluorescence staining results of the other groups are calculated. The lower the fluorescence value, the stronger the antioxidant capacity of the human dermal fibroblasts.

[0153] Table 4

[0154] Group Relative fluorescence value Model control group 672.44 Composition 1 138.21 Composition 2 147.52 Composition 3 142.39 Composition 1 276.32 Composition 2 304.51 Composition 3 298.46 Composition 4 264.37 Composition 5 187.61 Composition 6 205.49

[0155] Based on the results in Table 4, it can be seen that, compared with the model control group and compositions 1-3 and compositions ①-⑤, the compositions can effectively improve the antioxidant capacity of human dermal fibroblasts. Comparing the results of composition 1 and compositions ①-④, it can be seen that the crocus sativus extract, kangaroo paw extract, dendrobium stem extract, and adenosine in the composition defined in the present application have a certain synergistic effect, and their joint use can improve the antioxidant capacity of human dermal fibroblasts. Comparing the results of composition 1 and composition ⑤, it can be seen that, compared with the dendrobium stem extract, the synergistic effect between the dendrobium stem extract, crocus sativus extract, kangaroo paw extract, and adenosine is better. Comparing the results of composition 1 and composition ⑥, it can be seen that, when the crocus sativus extract, kangaroo paw extract, dendrobium stem extract, and adenosine are compounded in the mass ratio defined in the present application, the composition obtained has the best effect on improving the antioxidant capacity of human dermal fibroblasts.

[0156] Test 5: safety test of the serum

[0157] Test samples: serum 1-3 and serum ①-⑦.

[0158] The 2015 Cosmetic Safety Technical Specification was used as a reference standard for cosmetic irritation evaluation. The test method was a skin patch test, and 100 people aged 18-45 years old were randomly distributed for the test.

[0159] Test method: the test substance was placed in the patch tester, and the amount was 0.020-0.025 g. The patch tester with the test substance was covered with a non-irritating cloth-based adhesive tape on the back or forearm of the subject, and the palm was pressed to make it evenly attached to the skin surface. After 24 hours, 30 minutes after the test patch was removed, the skin reaction was observed after the indentation disappeared. If the result is negative, observe again at 24 hours and 48 hours after the patch test.

[0160] Evaluation criteria:

[0161] 0: negative reaction;

[0162] 1: suspicious reaction, only weak erythema;

[0163] 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0164] 3: strong positive reaction, erythema, infiltration, edema, and papules may be present;

[0165] 4: very strong positive reaction, obvious erythema, severe infiltration, edema, and confluent blisters, and the reaction exceeds the test area.

[0166] Test results: the skin reactions of all subjects were negative.

[0167] Test 6: effect of the serum on skin elasticity

[0168] Test samples: serum 1-3 and serum ①-⑦.

[0169] The eye cream prepared in the application was subjected to an 8-week anti-aging test according to the method recorded in the literature (Zhu Liping et al., Ginseng Anti-aging Mask Clinical Efficacy Test and Analysis [J], Chinese Cosmetology Medicine, 2016, 25(02): 33-36).

[0170] Each essence selects 10 volunteers. Inclusion criteria: ① men and women, aged 30-65 years old; have certain facial / ocular skin relaxation or fine wrinkles on the face / eyes; ② physically healthy, serious attitude, have certain expression ability, can truly reflect the feeling after use; ③ voluntarily participate, and sign the informed consent, can strictly follow the requirements of the research scheme, use the product according to the research scheme and complete the follow-up. Exclusion criteria: ① pregnant or lactating women; ② sensitive skin; ③ facial skin damage or skin disease affecting the observation of facial state; ④ facial skin treatment, cosmetic and other tests that may affect the results; ⑤ sunburn or use of hormone drugs and immunosuppressants within the past 1 month; ⑥ current or within the past 3 months, participated in other facial clinical studies or received dermatologist treatment. Termination and exclusion criteria: ① the subject requires termination of the trial; ② the subject's compliance is poor; ③ due to adverse reactions or special physiological and pathological changes, etc., cannot continue the trial; ④ during the test, use other skin care products that have an impact on this experiment, receive excessive ultraviolet radiation, etc.

[0171] Product usage method: volunteers use 500-600mg of the test sample evenly on the face after cleansing every night, massage and absorb without cleansing and applying any other cosmetics, for 8 consecutive weeks: at 0 and 8 weeks, measure the relevant data on the same part.

[0172] Facial skin elasticity measurement: use a skin elasticity tester to measure the skin elasticity R2 of the left and right eye tails and under-eyes; R2=Ua / Uf; Ua is the recovery value of the skin from canceling negative pressure to the next continuous test of the skin surface and adding negative pressure; Uf is the maximum stretching amount of the skin under negative pressure.

[0173] Skin elasticity improvement rate (%)=(R2 after use-R2 before use) / R2 before use x 100%

[0174] Table 5

[0175] Group Skin elasticity improvement rate (%) Serum 1 27.42 Serum 2 25.39 Serum 3 25.97 Serum 1 16.44 Serum 2 12.57 Serum 3 15.31 Serum 4 14.22 Serum 5 19.07 Serum 6 21.94 Serum 7 3.15

[0176] Based on the results in Table 5, it can be seen that compared with essence 1-3 and essence ⑦, the composition can effectively improve the skin elasticity, compared with essence 1 and essence ①-④, it can be seen that the crocus sativus extract, wallaby paw extract, dendrobium stem extract and adenosine in the composition defined in the application have a certain synergistic effect, and the combination can effectively improve the skin elasticity; compared with essence 1 and essence ⑤, it can be seen that compared with the dendrobium stem extract, the synergistic effect between the dendrobium stem extract, the crocus sativus extract, the wallaby paw extract and the adenosine is better; compared with essence 1 and essence ⑥, it can be seen that the crocus sativus extract, the wallaby paw extract, the dendrobium stem extract and the adenosine are compounded in the mass ratio defined in the application, and the composition obtained has a better effect on improving the skin elasticity.

[0177] The embodiments described above are only part of the embodiments of the present application, rather than all the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application claimed, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative labor are within the scope of protection of the present application.

Claims

1. An anti-inflammatory and anti-aging composition, characterized in that, Includes the following quantities of raw materials: Saffron extract: 0.2-0.7 parts; Kangaroo paw extract: 0.2-0.5 parts; Dendrobium nobile stem extract: 0.2-1 part; Adenosine: 1-5 parts; The saffron extract, kangaroo paw extract, and Dendrobium nobile stem extract are all water-soluble.

2. The composition according to claim 1, characterized in that, Includes the following quantities of raw materials; Saffron extract: 0.3-0.6 parts; Kangaroo paw extract: 0.3-0.4 parts; Dendrobium nobile stem extract: 0.5-0.8 parts; Adenosine: 2-4 parts.

3. The composition according to claim 1, characterized in that, Includes the following quantities of raw materials; Saffron extract: 0.5 parts; Kangaroo paw extract: 0.4 parts; Dendrobium nobile stem extract: 0.7 parts; Adenosine: 3 parts.

4. The use of an anti-inflammatory and anti-aging composition as described in any one of claims 1-3 in the preparation of skin care products with anti-inflammatory and anti-aging effects.

5. The application according to claim 4, characterized in that, The skincare product formulation is any one of serum, cream, emulsion, or mask.

6. An essence with anti-inflammatory and anti-aging effects, characterized in that, It comprises the anti-inflammatory and anti-aging composition according to any one of claims 1-3.

7. The essence according to claim 6, characterized in that, The essence also includes solvents, pH adjusters, and preservatives.

8. The essence according to claim 7, characterized in that, The solvent is deionized water; the pH adjuster is at least one of arginine or disodium hydrogen phosphate; the preservative is at least one of p-hydroxyacetophenone, 1,2-hexanediol, and 1,2-pentanediol.

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