Method for extracting coenzyme Q 10 from tobacco

A technology for coenzyme and tobacco leaves, which is applied in chemical instruments and methods, preparation of organic compounds, separation/purification of hydroxyl compounds, etc., can solve the problems of high price, high cost, unsuitable for large-scale promotion, etc. The effect of improving capacity, improving yield and maintaining color

Inactive Publication Date: 2016-05-11
广西北部湾制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the cost is high, the price is expensive, and it is not suitable for large-scale industrialization

Method used

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  • Method for extracting coenzyme Q 10 from tobacco
  • Method for extracting coenzyme Q 10 from tobacco
  • Method for extracting coenzyme Q 10 from tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for extracting coenzyme Q10 from tobacco leaves specifically comprises the following steps:

[0027] 1) Pretreatment: add the same weight of pure water to the tobacco leaf extract obtained by extracting the waste tobacco leaves, and process it through high-pressure micro-jet ultrafine pulverization, and use a high-speed shear emulsifier for 3 minutes at a speed of 12000r / min, and then use The high-pressure micro-jet ultrafine pulverization equipment is used for processing at 25°C, the processing pressure is 80MPa, and the processing times are 3 times to obtain a paste-like slurry, and then use NaOH ethanol solution and petroleum ether system to dynamically saponify the bound solanesol, crystallize and filter , to obtain an extract containing solanesol;

[0028] 2) Molecular distillation: the extract containing solanesol obtained in step 1) was removed by molecular distillation with a vacuum degree of 0.01Pa, a distillation temperature of 190°C, a scraper spee...

Embodiment 2

[0033] The method for extracting coenzyme Q10 from tobacco leaves specifically comprises the following steps:

[0034]1) Pretreatment: add the same weight of pure water to the tobacco leaf extract obtained by extracting the waste tobacco leaves, and process it through high-pressure micro-jet ultrafine pulverization, and process it with a high-speed shear emulsifier for 5 minutes at a speed of 8000r / min, and then use The high-pressure micro-jet ultrafine pulverization equipment is used for treatment at 25°C, the treatment pressure is 120MPa, and the treatment times are 2 times to obtain a paste-like slurry, and then use NaOH ethanol solution and petroleum ether system to dynamically saponify the bound solanesol, crystallize and filter , to obtain an extract containing solanesol;

[0035] 2) Molecular distillation: the extract containing solanesol obtained in step 1) was removed by molecular distillation with a vacuum degree of 0.01Pa, a distillation temperature of 190°C, a scra...

Embodiment 3

[0040] The method for extracting coenzyme Q10 from tobacco leaves specifically comprises the following steps:

[0041] 1) Pre-treatment: add the same weight of pure water to the tobacco leaf extract obtained by extracting the waste tobacco leaves, and process it through high-pressure micro-jet ultrafine pulverization, and use a high-speed shear emulsifier for 4 minutes at a speed of 10000r / min, and then use The high-pressure micro-jet ultrafine pulverization equipment is used for processing at 25°C, the processing pressure is 100MPa, and the processing times are 3 times to obtain a paste-like slurry, and then use NaOH ethanol solution and petroleum ether system to dynamically saponify the bound solanesol, crystallize and filter , to obtain an extract containing solanesol;

[0042] 2) Molecular distillation: the extract containing solanesol obtained in step 1) was removed by molecular distillation with a vacuum degree of 0.01Pa, a distillation temperature of 190°C, a scraper sp...

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Abstract

The invention discloses a method for extracting coenzyme Q 10 from tobacco, which comprises the following steps: (1) pretreatment: adding pure water into tobacco extract extracted from waste inferior tobacco according to same weight, obtaining pasty size by high pressure microjet superfine grinding treatment, then by saponification, crystallizing and filtering, obtaining extract containing solanesol; (2) molecular distillation: purifying the extract containing solanesol in step 1 by molecular distillation to obtain light phase solanesol and heavy phase solanesol; (3) column chromatography separation: dissolving and filtering the heavy phase solanesol in step 2 with acetone, collecting eluant by column chromatography separation, and concentrating to obtain the molecular distilled and purified solanesol; (4) preparation of methyl dimethoxy benzoquinone: performing bromo-etherification and oxidation on methylphenol to obtain the methyl dimethoxy benzoquinone; (5) mixing the light phase solanesol in step 2, the molecular distilled and purified solanesol in step 3 and the methyl dimethoxy benzoquinone in step 4 and reacting to obtain the coenzyme Q 10.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting coenzyme Q10 from tobacco leaves. Background technique [0002] Coenzyme Q10, English name: COENZYMEQ10, CASNo.303-98-0, molecular formula C 59 h 90 o 4 , molecular weight 863.36, yellow to orange crystalline powder, odorless and tasteless, easy to decompose when exposed to light, slightly soluble in ethanol, insoluble in water. Ubiquinone 10, also known as ubiquinone 10, is a fat-soluble quinone whose structure is similar to vitamin K. ring compounds. Coenzyme Q10 is one of the indispensable and important elements of human life. It can activate the nutrition of human cells and cell energy. It has the functions of improving human immunity, enhancing anti-oxidation, delaying aging and enhancing human vitality. It is widely used in the cardiovascular system in medicine. diseases, it is widely used in nutritional health products and f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C46/00C07C50/28C07C29/76C07C33/02C07C29/80
CPCC07C46/00C07C29/74C07C29/76C07C29/80C07C50/28C07C33/02
Inventor 劳雪红
Owner 广西北部湾制药股份有限公司
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