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Method for extracting polyoses from bagasse

A bagasse and polysaccharide technology, applied in the field of preparation of polysaccharide compounds, can solve problems such as difficult to obtain good results, and achieve the effects of less solvent consumption, high extraction rate and mild reaction conditions

Inactive Publication Date: 2008-05-07
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Ganoderma lucidum is a fungus, and the polysaccharides of Ganoderma lucidum are mostly stored in the cell wall of the fruiting body. The main components of the fruiting body are cellulose, hemicellulose and lignin, while sugarcane is a plant, and bagasse is mainly composed of whole cellulose, lignin, polysaccharides, etc. It is composed of pentosan, etc., and its fibers mainly include true fiber and sugarcane pith. True fiber has a relatively tough hard wall tissue, and sugarcane pith is like a spongy structure, which is quite different from Ganoderma lucidum. It is difficult to obtain it by applying the above method. Good results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Add 8 liters of pure ethanol to 1 kg of bagasse coarse powder, reflux for extraction for 1 hour, and filter to collect the filter residue. Add 10 times of water, add 15 grams of cellulase, adjust the pH value to 5.5, mix with the filter residue, keep warm at 60°C for 60 minutes, ultrasonically treat for 60 minutes, filter, the filtrate is the polysaccharide extract, add ethanol to adjust the alcohol after the extract is concentrated The concentration is 90%, left overnight, the precipitate is vacuum-dried at 60° C., and pulverized to obtain 85 g of crude bagasse polysaccharide.

Embodiment 2

[0019] Add 10 liters of 90% ethanol to 1 Kg of bagasse coarse powder, reflux and extract for 2 hours, and filter to collect the filter residue. Add 12 times of water, add 5 grams of cellulase, adjust the pH value to 4.0, mix with the filter residue, incubate at 80°C for 30 minutes, ultrasonically treat for 90 minutes, filter, the filtrate is polysaccharide extract, add ethanol to adjust the alcohol after the extract is concentrated The concentration was 85%, left overnight, the precipitate was vacuum-dried at 70° C., and pulverized to obtain 65 g of crude bagasse polysaccharide.

Embodiment 3

[0021] Add 10 liters of 90% ethanol to 1 Kg of bagasse coarse powder, reflux and extract for 2 hours, and filter to collect the filter residue. Add 12 times of water, add 10 grams of cellulase, adjust the pH value to 6.0, mix with the filter residue, incubate at 40°C for 90 minutes, ultrasonicate for 30 minutes, filter, the filtrate is the polysaccharide extract, add ethanol to adjust the alcohol after the extract is concentrated The concentration was 85%, left overnight, the precipitate was vacuum-dried at 70° C., and pulverized to obtain 64 g of crude bagasse polysaccharide.

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PUM

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Abstract

The invention provides a method for extracting polysaccharides from bagasse, the method is: after degreasing the bagasse, using one or more of cellulase, papain and pectinase at pH 4-6, Hydrolyze at 40-80°C for 0.5-1.5 hours, then treat with ultrasonic waves with a frequency of 59KHz for 0.5-1.5 hours, filter, and finally use ethanol to precipitate polysaccharides in the filtrate. The method of the invention adjusts the type and amount of enzymes and the technological conditions of enzyme treatment and ultrasonic extraction on the basis of the process of extracting fungal polysaccharides, and finds a low energy consumption and high-efficiency method for extracting bagasse polysaccharides.

Description

technical field [0001] The invention relates to the preparation of polysaccharide compounds, in particular to the extraction of bagasse polysaccharides. Background technique [0002] Bagasse is the main by-product of the sugar industry. It is the main part left after sugar cane is mechanically pressed. It is a kind of agricultural solid waste. Its composition is similar to that of wood materials and can be used as a raw material to replace part of wood. Hainan, Guangdong, Guangxi, Fujian, Yunnan and other provinces in China can grow sugarcane, and are also important sugar-producing provinces. Every sugar factory has a large amount of bagasse in stock, so it is of great significance to find a suitable way to comprehensively utilize bagasse. [0003] Bagasse contains polysaccharides, mainly composed of five-carbon sugars and six-carbon sugars. Studies have shown that the polysaccharides extracted from bagasse have immune enhancement, anti-tumor, anti-virus, hypoglycemic, hypo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/09
Inventor 刘强刘莉
Owner SOUTHERN MEDICAL UNIVERSITY
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