34results about How to "Extract fit" patented technology

The invention provides a method for extracting polysaccharides from bagasse, the method is: after degreasing the bagasse, using one or more of cellulase, papain and pectinase at pH 4-6, Hydrolyze at 40-80°C for 0.5-1.5 hours, then treat with ultrasonic waves with a frequency of 59KHz for 0.5-1.5 hours, filter, and finally use ethanol to precipitate polysaccharides in the filtrate. The method of the invention adjusts the type and amount of enzymes and the technological conditions of enzyme treatment and ultrasonic extraction on the basis of the process of extracting fungal polysaccharides, and finds a low energy consumption and high-efficiency method for extracting bagasse polysaccharides.

The invention discloses a pure natural plant skin-care product. The pure natural plant skin-care product disclosed by the invention is prepared from watery stoste directly extracted from natural mesona chinensis benth. A preparation process of the pure natural plant skin-care product comprises the following steps: rinsing fresh mesona chinensis benth leaves by using deionized water and airing; immersing by using clear water for 24 h under the conditions as follows: the TDS (Total Dissolved Solid) is less than 10 mg / L, the pH is 6.6-7.1, and the nuclear magnetic resonance half-width amplitude is 80-100 Hz; adding the immersed mesona chinensis benth together with immersing solution into a distillation boiler, putting the immersing solution at the bottom of the distillation boiler, and paving mesona chinensis benth above the immersing solution; and starting a high-pressure distiller, and distilling, condensing, decolouring and filtering so as to obtain the watery stoste of mesona chinensis benth. The watery stoste of natural mesona chinensis benth is used as the raw material of the pure natural plant skin-care product; the pure natural plant skin-care product disclosed by the invention integrates the effects of preserving moisture, resisting wrinkle, inhibiting stain and pox, whitening skin, softening cutin and moisturizing and protecting hair; if the pure natural plant skin-care product is used for a long time, skin lesion or property change cannot be caused; and in addition, the pure natural plant skin-care product also does not have irritation or negative effects even being dropped in eyes or taken orally.

The invention relates to an extractant used for extracting extracellular vesicle in body fluid and an extracting method thereof. The extractant is prepared from inorganic salt and a water-soluble polymer, the inorganic salt is selected from one or more of ammonium sulfate, calcium chloride, sodium chloride, magnesium chloride or sodium citrate, and the water-soluble polymer is selected from one ofpolyethylene glycol PEG, methylcellulose MC and polyethyleneimine PEI. The extracting method comprises the steps that the extractant is mixed with the body fluid to form a to-be-extracted system, andafter salting-out and centrifugation, the extracellular vesicle can be extracted out. The adopted salting-out method for extracting the extracellular vesicle has the three advantages that 1), the yield is high and is about 70%; 2) the operation is quick and convenient, the extracellular vesicle can be extracted out within 30-45 minutes; 3) the method is not limited by the size of a sample and issuitable for extracting of samples with large-sizes.

The invention provides an opopanax extract. The extract is prepared by extracting opopanax by a polar solvent, adsorbing the crude extract by virtue of adsorption resin, and eluting by eluent composed of water and ethanol. The opopanax extract is prepared by using a single raw material opopanax, is simple and effective, is capable of inhibiting the activity of tyrosinase, can be applied to cosmetics, and can be used for producing foods, animal feeds, medicines, quasi-drugs and the like.

The invention particularly relates to a kit for quickly extracting double-stranded RNA of a mycovirus and application of the kit. The kit comprises a cell lysis buffer, a nucleic acid adsorption solution, a nucleic acid cleaning solution and a double-stranded RNA agarose gel electrophoresis reagent. According to the invention, a new buffer solution system is adopted, a silicon film is used to replace cellulose powder to be used as an adsorption medium, organic reagents such as phenol are not required, through the reactions of the cell lysis buffer and the nucleic acid adsorption solution, impurities such as proteins, most DNA and polyose are removed in a chemical precipitation manner, and finally, by adjusting adsorption conditions through alcohol, the double-stranded RNA is effectively combined with the silicon film and the purified double-stranded RNA is obtained through elution. Through the adoption of the kit, high-quality double-stranded RNA can be prepared from a hypha sample with the weight as low as 0.3 g, and the whole extraction process is conducted within one hour; steps of organic reagent extraction and supernatant suction in a conventional method are eliminated, and all operations related to column-passing and the like are completed through centrifugation. The kit is suitable for high flux treatment of a large number of samples.

The invention discloses a method for extracting marine animal phospholipids by ultrasonic reflux. The method comprises the following steps: (1) sample pretreatment: homogenating and freeze-drying visceral organs of marine animals, and screening the visceral organs by a screen of 60-80 meshes to prepare freeze-dry powder; (2) ultrasonic reflux extraction: adding a solvent into a liquid storage tank of ultrasonic reflux extraction equipment, heating to be 40-45 DEG C, feeding baits from a material inlet according to a material-to-liquid ratio of 1: 6-1: 10, performing reflux extraction by a spiral propeller, switching on ultrasonic waves, and opening a liquid outlet to enable extracting solution to enter the liquid storage tank; (3) concentration: performing rotary evaporation on the extracting solution to concentrate to a thick material; (4) deoiling: adding acetone into the concentrated thick material for digestion until the solvent is colorless, and performing suction filtration; (5) drying: performing vacuum drying on a filtering cake to obtain the marine animal phospholipids. Compared with the conventional phospholipids extraction method, the method disclosed by the invention has the advantages that the extraction rate and the extraction efficiency are higher, the use amount of ethyl alcohol can be reduced, and energy can be saved; the extraction temperature is reduced, and the active function of a product cannot be lost.

The invention relates to a detection method for the perfuming uniformity of cigarette shreds. The method sequentially comprises the following steps: A. confirming a label of characterization uniformity; B. sampling from an exit of a cigarette shred perfuming roller; C. balancing the cigarette shred sample obtained by sampling; D. pre-treating the cigarette shred sample by extraction with a rapid solvent extraction instrument, and preparing a filtering medium; E. carrying out gas chromatography and mass spectrum combined analysis on the filtering medium, taking the label as the analysis object, and adopting a selected ion scanning pattern; and F. calculating the content of the label in the cigarette shred sample, obtaining a standard deviation (SD), and reflecting the perfuming uniformity degree of the cigarette shred by the SD value. The detection method adopts the gas chromatography / mass spectrum instrument as the separation and analysis means, and quantifying-researches the uniformity of the perfuming procedure of the cigarette shreds, thereby confirming the uniformity of cigarette shred perfuming. The detection method is applied to the perfuming technological process in the cigarette production, thereby providing a practical and scientific method for the detection of the cigarette perfuming precision.

The invention provides a polyhexose extraction method from the sugar cane bagasse. The method is realized by that after the degreasing processing is performed to the sugar cane bagasse, one or more of cellulolytic enzyme, papayotin and pectolytic enzyme can be hysrolyzed for 0.5 to 1.5 hours under pH value of 4 to 6 at the temperature of 40 to 80 DEG C, treated for 0.5 to 1.5 hours under the supersonic wave with the frequency of 59KHz, and filtered, and finally the polyhexose in the filtrate water is precipitated through ethanol. The method of the invention adjusts the types and the dose of the enzyme on the technology base of eumycete polyhexose extraction, and finds a low energy consumption and efficient sugar cane bagasse polyhexose extraction method under the enzyme processing and supersonic wave extraction technology.

The embodiment of the invention provides a document information extraction method and a device. The method comprises the steps of determining an area serving as an anchor point area in a to-be-extracted document image, adjusting an image with a to-be-extracted contour to be in the size of a preset template image, obtaining a target image, adjusting the area to be in the size of a preset template anchor point, and obtaining a target anchor point; determining the position of the target anchor point in the target image, and obtaining the position of the region of interest in the target image by combining the pre-defined region of interest of the template image with the relative position of the template anchor point; according to the size of a pre-defined region of interest in the template image, in combination with the position of the region of interest, extracting the region of interest from the target image; wherein the template image is defined according to a document image to be extracted. The embodiment of the invention is particularly suitable for extracting document information in a large number of document images with fixed formats.

The invention relates to the technical field of biology, and particularly discloses a method for extracting Chinese eaglewood essential oil. The method comprises the steps of crushing a Chinese eaglewood raw material, adding water, and stirring to obtain slurry; processing the slurry by a colloid mill; performing ultrasonic treatment on the slurry and hydrolyzing by using cellulase; performing enzyme inactivation and suction filtration after hydrolysis, extracting the prepared first filtrate by using 95% ethanol, performing suction filtration again on the organic phase, distilling prepared second filtrate for removing ethanol, thus obtaining the Chinese eaglewood essential oil. According to the method disclosed by the invention, the Chinese eaglewood essential oil is subjected to double-effect extraction by using ultrasonic waves and an enzymolysis method, so that the extraction rate of the Chinese eaglewood essential oil is effectively increased to ensure that the Chinese eaglewood essential oil extraction rate of the Chinese eaglewood raw material containing a whitewood layer is increased up to be more than 0.4%, and meanwhile, the extraction time is shortened; the method is suitable for extracting the Chinese eaglewood essential oil of natural Chinese eaglewood and artificial Chinese eaglewood.

The invention particularly relates to a kit for quickly extracting double-stranded RNA of a mycovirus and application of the kit. The kit comprises a cell lysis buffer, a nucleic acid adsorption solution, a nucleic acid cleaning solution and a double-stranded RNA agarose gel electrophoresis reagent. According to the invention, a new buffer solution system is adopted, a silicon film is used to replace cellulose powder to be used as an adsorption medium, organic reagents such as phenol are not required, through the reactions of the cell lysis buffer and the nucleic acid adsorption solution, impurities such as proteins, most DNA and polyose are removed in a chemical precipitation manner, and finally, by adjusting adsorption conditions through alcohol, the double-stranded RNA is effectively combined with the silicon film and the purified double-stranded RNA is obtained through elution. Through the adoption of the kit, high-quality double-stranded RNA can be prepared from a hypha sample with the weight as low as 0.3 g, and the whole extraction process is conducted within one hour; steps of organic reagent extraction and supernatant suction in a conventional method are eliminated, and all operations related to column-passing and the like are completed through centrifugation. The kit is suitable for high flux treatment of a large number of samples.

The present invention provides a method and system for extracting a building area based on a SAR image. The method for extracting a building area based on a SAR image includes: acquiring a first image based on a SAR image; generating a local spatial index image and a variogram based on the first image image; based on the local space index image, variogram image, and the first image, respectively filter the sampling points to obtain the corresponding sets; process the sampling points in each set according to the preset growth criteria to construct the images of the building areas to be selected, and divide each Select the image of the built-up area to superimpose to obtain the final image of the built-up area. The method or system provided by the invention can quickly and accurately extract building areas from Sentinel-1 SAR images, realize automatic extraction of building land in large areas, and lay a foundation for global building area mapping.

The invention relates to a technique for extracting effective ingredients from a natural product, particularly a technique for extracting resveratrol and pigment from grape skin, which comprises the following steps: drying the grape skin, pulverizing, extracting resveratrol by ultrasonic waves, extracting pigment by ultrasonic waves, and purifying to obtain the resveratrol product and the pigment product. In the technical scheme provided by the invention, double solvents and ultrasonic extraction are integrated; the technical scheme provided by the invention has the advantages of high extraction efficiency, low extraction cost and low energy consumption by using the ultrasonic extraction technique according to the principle that the resveratrol and pigment in the grape skin have different solubilities in solvents with different polarities; the solvents used in the extraction process can be utilized cyclically, so that the solvent consumption is low, and the environmental influence is low, thereby avoiding destroying the bioactivities of the resveratrol and the pigment in the extraction process; and thus, the invention is suitable for extracting bioactive substances.

The invention provides a method for extracting high-purity omega-3 polyunsaturated fatty acid phospholipids from shrimp heads. The method comprises the steps of homogenizing the shrimp heads, and carrying out enzymolysis by complex enzymes; carrying out solid-liquid separation, adding the solid part into an alkaline ethanol solution, carrying out ultrasonic-assisted extraction, filtering and concentrating to obtain an extract; and dissolving the extract, then carrying out polystyrene gel column chromatography, carrying out thin-layer chromatography detection analysis in combination with an eluent, carrying out reduced pressure condensation, washing by an ice ethanol solution, and then drying to obtain high-purity phospholipids. According to the method, the operation is simple, the extraction process is simple and easy to operate, the fixed adsorption amount of column chromatography fillers is few, repeated use can be achieved, the temperature is not more than 50 DEG C, the physiological activity of phospholipids cannot be damaged, and the purity of the extracted phospholipids is more than 95%. Meanwhile, the invention opens up a new approach for high-valued comprehensive development and utilization of marine product processing wastes, and raw materials are not limited in a certain source and can derive from multiple marine animal processing wastes.

The invention discloses a method for extracting uranidin from physalis pubescens fruit, and relates to a method for extracting uranidin, solving the problems that the conventional physalis pubescens uranidin extraction method is low in extraction rate and large in organic solvent consumption, a long time is needed, the uranidin is easy to oxidize and decompose in the extraction process, and industrial production cannot be achieved by means of supercritical extraction. The method disclosed by the invention comprises the following steps: firstly, juicing the physalis pubescens fruit; secondly, adding an organic solvent to perform ultrasonic-assisted extraction; thirdly, recycling and concentrating; fourthly, drying so as to obtain the uranidin. The method is high in extraction rate of the physalis pubescens uranidin in an ultrasonic extraction mode, the extraction rate is increased by 0.5-1.5% when being compared with an ordinary conventional ethanol leaching method, and moreover the extraction time is short, a small amount of the organic solvent is consumed, the condition is gentle, the extracted uranidin is not damaged, and theoretic basis is provided for industrial production of natural uranidin. The method is applied to extraction of uranidin in physalis pubescens fruit.

The response surface optimization method for extracting barley polyphenols belongs to the technical field of barley polyphenol extraction. The invention adopts an ultrasonic method to extract coix seed polyphenols, uses gallic acid as a standard product and folinol as a color developing agent, and measures the polyphenol content by a colorimetric method. The experiment investigated the effects of seven factors including solvent type, solid-liquid ratio, solvent volume fraction, extraction temperature, extraction time, extraction times, and sample particle size on the extraction rate of barley polyphenols. On the basis of the single factor experiment, the experimental scheme was designed by using the Box-Behnken response surface analysis method, and the material-liquid ratio, solvent volume fraction, extraction time, and extraction temperature were used as the experimental factors. The effectiveness of the regression model and the influence of each single factor were analyzed, and the factors in the extraction of polyphenols from the seed of Job's tears were optimized to obtain the optimized process conditions for the extraction of polyphenols from the seed of Job's tears. There is a good fit between the measured value and the theoretical value, which is suitable for the extraction of polyphenols from barley.

The invention discloses a method for quickly extracting low molecular weight RNA of plant. After cells are disrupted, genomic DNA and high molecular weight RNA form a DNA-protein complex and a RNA-protein complex with protein respectively; and the DNA-protein complex and the RNA-protein complex are insoluble in acid and low-salt environment, and can be removed and extracted through acid and phenol. The low molecular weight RNA stays in supernatant solution, and can be recovered through the precipitation of ethanol or isopropanol. The method is quick and efficient, and can recover the low molecular weight RNA only by one-step precipitation after phenol extraction. The low molecular weight RNA extracted by the method has high purity, and can be used for the cloning of the low molecular weight RNA and the detection of Northern blot hybridization. The method has simple and quick experimental process, and is suitable for the extraction of the low molecular weight RNA of various plant tissues.

The invention provides a method for extracting high-purity marine phospholipids from Euphausia superba; the method comprises the steps of homogenizing Euphausia superba, and enzymatically hydrolyzing with enzyme complex; carrying out solid-liquid separation, adding the solid portion to alkaline ethanol solution, and carrying out ultrasonic-assisted extraction; water-precipitating and deoiling, and carrying out polystyrene gel column chromatography; washing with glacial acetic acid solution to obtain high-purity phospholipids. The materials herein are enzymatically hydrolyzed with the enzyme complex of bromelain and chitinase, efficient extraction is carried out by using the alkaline ethanol solution, phospholipids extraction efficiency is improved, whole-course temperature is not higher than 50 DEG C, and biophysical activity of phospholipids is not higher than 95% which is significantly higher than that of the prior art.