34results about How to "Extract fit" patented technology

The invention belongs to the field of biological chemistry and specifically relates to extraction method of chloroplast protein of xylophyta ginkgo leaf. The method comprises the following steps of: firstly, preparing coarse chloroplast particles from the ginkgo leaves, performing gradient centrifugation through Percoll, so as to obtain complete chloroplast by a specific centrifugal force; then, dissolving protein by using a protein extraction buffer solution, adding the same volume of Tris-balanced phenol for extraction, and precipitating protein by using a ammonium acetate methanol solution. The extraction method is capable of effectively increase extraction efficiency and purity of chloroplast protein. The ginkgo leaf chloroplast protein obtained by the method can be used for proteomics analysis of ginkgo leaf and other xylophyta materials rich in interfering substances, so that solubility and purity of protein are improved; a 2-DE map of the protein has a clear background and many protein spots are distinguishable.

The invention belongs to the technical field of biology and discloses an extraction method of plant RNA (ribonucleic acid). The method comprises the following steps: sufficiently grinding the plant material in liquid nitrogen to powder, adding an extraction buffer A, sufficiently reacting, adding an extraction buffer B, evenly mixing to react, precipitating with isopropanol, separating out the RNA, washing with ethanol, and dissolving in RNase-free water. The method does not need to use DEPC or the expensive extraction kit, and does not need to use phenol, chloroform and other toxic irritating reagents; and the method is simple to operate and easy to implement, has short extraction time (not more than 30 minutes for the whole process) and has the advantages of favorable completeness and higher concentration as compared with the extracted RNA and kit. The method is more suitable for extracting RNA from polyphenol plants from which TRIZOL can not be extracted generally. The purity and concentration of the extracted RNA are sufficient for RNA reverse transcription, RT-PCR (reverse transcription-polymerase chain reaction) and Nothern blot hybrid experiments.

The invention discloses a method for quickly extracting low molecular weight RNA of plant. After cells are disrupted, genomic DNA and high molecular weight RNA form a DNA-protein complex and a RNA-protein complex with protein respectively; and the DNA-protein complex and the RNA-protein complex are insoluble in acid and low-salt environment, and can be removed and extracted through acid and phenol. The low molecular weight RNA stays in supernatant solution, and can be recovered through the precipitation of ethanol or isopropanol. The method is quick and efficient, and can recover the low molecular weight RNA only by one-step precipitation after phenol extraction. The low molecular weight RNA extracted by the method has high purity, and can be used for the cloning of the low molecular weight RNA and the detection of Northern blot hybridization. The method has simple and quick experimental process, and is suitable for the extraction of the low molecular weight RNA of various plant tissues.

The invention provides a building area extraction method and system based on an SAR image. The building area extraction method based on the SAR image includes: acquiring a first image on the basis ofthe SAR image; generating a local space index image and a variogram image on the basis of the first image; respectively screening sampling points on the basis of the local space index image, the variogram image and the first image to obtain corresponding sets; and processing sampling points in each set according to preset growth criteria to respectively construct to-be-selected building area images, superimposing all the to-be-selected building area images to obtain a final building area image. Through the method or the system provided by the invention, a building area can be quickly and accurately extracted from the Sentinel-1 SAR image, automatic extraction of construction land of a large region is realized, and a foundation is laid for global building area mapping.

The invention discloses oil-marinated food and a preparation method thereof. The oil-marinated food comprises the following raw materials in parts by weight: 1500-2000 parts of raw material meat, 250-420 parts of marinating oil, 50-100 parts of white granulated sugar, 50-100 parts of a chicken essence, 20-40 parts of monosodium glutamate, 50-100 parts of table salt and 100-150 parts of secret spices. By the preparation method, the freshness of a food material can be better locked, and water is continuously evaporated in the marinating process, so that juice separated out from the food materialcan be in a continuous concentrating reaction process, and the taste is richer and thicker.

The invention discloses a response surface optimized coix seed polyphenol extraction method, and belongs to the technical field of coix seed polyphenol extraction. According to the response surface optimized coix seed polyphenol extraction method, an ultrasonic method is adopted to extract coix seed polyphenol, gallic acid is taken as a standard substance, foline-phenol is taken as a color developing agent, and the content of polyphenol is tested by a colorimetric method. The effects of the seven factors of a solvent type, a solid-liquid ratio, a solvent volume fraction, extraction temperature, extraction time, extraction times and particle size of a sample on the extraction rate of the coix seed polyphenol are investigated by an experiment. On the basis of a single factor experiment, a Box-Behnken response surface analysis method is adopted to design an experiment scheme, the solid-liquid ratio, the solvent volume fraction, the extraction time and the extraction temperature are takenas experimental factors, the extraction rate of the coix seed polyphenol is a response value, a regression model is established, the validity of the regression model and the influence degree of various single factors are analyzed, various factors during the coix seed polyphenols extraction are optimized, and optimized process conditions of the coix seed polyphenol extraction are acquired. A good fitting degree exists between an actually tested value and a theoretical value so that the method is suitable for the coix seed polyphenols extraction.

The invention provides a method for separation and determination of arsenic in PM2.5. According to the method, nitric acid with a concentration of 1% is used as an extraction solvent, and high performance liquid chromatography and inductively coupled plasma mass spectrometer are used in combination for extraction and determination of arsenic forms in PM2.5. The method can completely separate arsenic of four different forms within 10 min, wherein the arsenic forms include trivalent arsenic [As(III)], pentavalent arsenic [As(IV)] monomethyl arsenic (MMA) and dimethyl arsenic (DMA); the detection limit of the method is in a range of 0.6 to 1 [mu]g/L; the precision degrees of As(III), DMA, MMA and As(IV) are 1.26%, 1.24%, 1.00% and 0.79%, respectively; and obvious conversion among the four arsenic forms does not occur during determination.

The invention provides Chufeng ointment and an application thereof in treatment of maternal nipple cracking. The Chufeng ointment comprises, by weight, 1 parts of an egg yolk oil extract, 0.5-1.5 parts of sesame oil and 1.5-2.5 parts of pure natural lanolin. The preparation method of the egg yolk oil includes: boiling an egg, peeling the egg to take the yolk, crushing the yolk, performing cold leaching or ultrasonic extraction with food-grade ethyl acetate, and depressing an obtained extracting solution to have ethyl acetate recovered to obtain the egg yolk extract. The Chufeng ointment prepared with the method is applicable to treatment of maternal nipple cracking, and an indigenous method having a long history of medication in traditional Chinese medicine books is displayed in a modern way; the preparation method of the Chufeng ointment is simple in process, the Chufeng ointment is suitable for the special group of mothers and babies at the particular period of the suckling period and free from side effects for the mothers and babies, breast-feeding is unaffected, and safety and effectiveness are achieved.

The invention provides a method for extracting a food natural pigment from green plum pericarps. The method comprises the following steps: selecting and washing fresh green plums; heating the fresh green plums by adopting microwaves and dissolving into oversaturated table salt; adding a complex enzyme composed of cellulase and pectinase and carrying out enzymolysis; crystallizing and separating out at low temperature by adopting the dissolved table salt; then extracting the pigment by adopting an ultrasonic extraction manner. According to the method provided by the invention, the extraction efficiency and the extraction rate of the pigment are effectively improved and the pigment has extremely high food safety. The problems that a product produced by a traditional natural pigment extraction method has poor quality, low concentration, undesirable odor and residual solvent are overcome.

The invention provides a method for rapidly obtaining drug-resistant genes of probiotics in a sterilized dairy product based on a high-throughput sequencing technology, which comprises the following steps of: extracting DNA (Deoxyribose Nucleic Acid) of the sterilized dairy product, and then analyzing the drug-resistant genes carried by the probiotics in the sterilized dairy product by combining the high-throughput sequencing technology with a bioinformatics method. According to the method for rapidly obtaining the drug-resistant genes of the probiotics in the sterilized dairy product based on the high-throughput sequencing technology, the drug-resistant bacteria genes of the lactic acid bacteria in the sterilized dairy product can be efficiently and rapidly screened, and the method has a good application prospect.

The invention relates to a technology for extracting effective ingredients from natural products and extracting resveratrol from mulberries. The technology comprises the steps that the mulberries are dried and smashed, and resveratrol is extracted through ultrasonic waves and purified to obtain the resveratrol. According to the technical scheme of the method, dual solvent and ultrasonic extraction and integration are adopted, the characteristic that the solubleness of the resveratrol in the mulberries is higher in weak polar solvent is utilized, and an ultrasonic extraction technology is utilized, so that the technology has the advantages of being high in extraction efficiency and product purity and low in extraction cost and energy consumption; the solvent used in the extraction process can be recycled and little in usage amount, so that the influence to the environment is lower, and the biological activity of the resveratrol is prevented from being destroyed in the extraction process.

The invention discloses a resume information extraction method and system based on deep learning. The method comprises the following steps: collecting Chinese corpora of resumes, and taking the Chinese corpora as training data; constructing a neural network model according to information of the Chinese corpora; training the constructed neural network model through the training data until convergence; and inputting resumes into the neural network for information extraction. The method and the system have the beneficial effects that complex semi-structured data in resumes can be well dealt with when the method provided by the invention is used for extracting resume information; due to the fact that deep learning has strong characterization capabilities, features can be well extracted; and meanwhile, the method also has good generalization ability and is suitable for information extraction.