34results about How to "Extract fit" patented technology

The invention provides a method for extracting polysaccharides from bagasse, the method is: after degreasing the bagasse, using one or more of cellulase, papain and pectinase at pH 4-6, Hydrolyze at 40-80°C for 0.5-1.5 hours, then treat with ultrasonic waves with a frequency of 59KHz for 0.5-1.5 hours, filter, and finally use ethanol to precipitate polysaccharides in the filtrate. The method of the invention adjusts the type and amount of enzymes and the technological conditions of enzyme treatment and ultrasonic extraction on the basis of the process of extracting fungal polysaccharides, and finds a low energy consumption and high-efficiency method for extracting bagasse polysaccharides.

The invention belongs to the field of biological chemistry and specifically relates to extraction method of chloroplast protein of xylophyta ginkgo leaf. The method comprises the following steps of: firstly, preparing coarse chloroplast particles from the ginkgo leaves, performing gradient centrifugation through Percoll, so as to obtain complete chloroplast by a specific centrifugal force; then, dissolving protein by using a protein extraction buffer solution, adding the same volume of Tris-balanced phenol for extraction, and precipitating protein by using a ammonium acetate methanol solution. The extraction method is capable of effectively increase extraction efficiency and purity of chloroplast protein. The ginkgo leaf chloroplast protein obtained by the method can be used for proteomics analysis of ginkgo leaf and other xylophyta materials rich in interfering substances, so that solubility and purity of protein are improved; a 2-DE map of the protein has a clear background and many protein spots are distinguishable.

The invention belongs to the technical field of biology and discloses an extraction method of plant RNA (ribonucleic acid). The method comprises the following steps: sufficiently grinding the plant material in liquid nitrogen to powder, adding an extraction buffer A, sufficiently reacting, adding an extraction buffer B, evenly mixing to react, precipitating with isopropanol, separating out the RNA, washing with ethanol, and dissolving in RNase-free water. The method does not need to use DEPC or the expensive extraction kit, and does not need to use phenol, chloroform and other toxic irritating reagents; and the method is simple to operate and easy to implement, has short extraction time (not more than 30 minutes for the whole process) and has the advantages of favorable completeness and higher concentration as compared with the extracted RNA and kit. The method is more suitable for extracting RNA from polyphenol plants from which TRIZOL can not be extracted generally. The purity and concentration of the extracted RNA are sufficient for RNA reverse transcription, RT-PCR (reverse transcription-polymerase chain reaction) and Nothern blot hybrid experiments.

The invention discloses a method for quickly extracting low molecular weight RNA of plant. After cells are disrupted, genomic DNA and high molecular weight RNA form a DNA-protein complex and a RNA-protein complex with protein respectively; and the DNA-protein complex and the RNA-protein complex are insoluble in acid and low-salt environment, and can be removed and extracted through acid and phenol. The low molecular weight RNA stays in supernatant solution, and can be recovered through the precipitation of ethanol or isopropanol. The method is quick and efficient, and can recover the low molecular weight RNA only by one-step precipitation after phenol extraction. The low molecular weight RNA extracted by the method has high purity, and can be used for the cloning of the low molecular weight RNA and the detection of Northern blot hybridization. The method has simple and quick experimental process, and is suitable for the extraction of the low molecular weight RNA of various plant tissues.

The invention discloses a pure natural plant skin-care product. The pure natural plant skin-care product disclosed by the invention is prepared from watery stoste directly extracted from natural mesona chinensis benth. A preparation process of the pure natural plant skin-care product comprises the following steps: rinsing fresh mesona chinensis benth leaves by using deionized water and airing; immersing by using clear water for 24 h under the conditions as follows: the TDS (Total Dissolved Solid) is less than 10 mg / L, the pH is 6.6-7.1, and the nuclear magnetic resonance half-width amplitude is 80-100 Hz; adding the immersed mesona chinensis benth together with immersing solution into a distillation boiler, putting the immersing solution at the bottom of the distillation boiler, and paving mesona chinensis benth above the immersing solution; and starting a high-pressure distiller, and distilling, condensing, decolouring and filtering so as to obtain the watery stoste of mesona chinensis benth. The watery stoste of natural mesona chinensis benth is used as the raw material of the pure natural plant skin-care product; the pure natural plant skin-care product disclosed by the invention integrates the effects of preserving moisture, resisting wrinkle, inhibiting stain and pox, whitening skin, softening cutin and moisturizing and protecting hair; if the pure natural plant skin-care product is used for a long time, skin lesion or property change cannot be caused; and in addition, the pure natural plant skin-care product also does not have irritation or negative effects even being dropped in eyes or taken orally.

The invention relates to an extractant used for extracting extracellular vesicle in body fluid and an extracting method thereof. The extractant is prepared from inorganic salt and a water-soluble polymer, the inorganic salt is selected from one or more of ammonium sulfate, calcium chloride, sodium chloride, magnesium chloride or sodium citrate, and the water-soluble polymer is selected from one ofpolyethylene glycol PEG, methylcellulose MC and polyethyleneimine PEI. The extracting method comprises the steps that the extractant is mixed with the body fluid to form a to-be-extracted system, andafter salting-out and centrifugation, the extracellular vesicle can be extracted out. The adopted salting-out method for extracting the extracellular vesicle has the three advantages that 1), the yield is high and is about 70%; 2) the operation is quick and convenient, the extracellular vesicle can be extracted out within 30-45 minutes; 3) the method is not limited by the size of a sample and issuitable for extracting of samples with large-sizes.

The invention provides a building area extraction method and system based on an SAR image. The building area extraction method based on the SAR image includes: acquiring a first image on the basis ofthe SAR image; generating a local space index image and a variogram image on the basis of the first image; respectively screening sampling points on the basis of the local space index image, the variogram image and the first image to obtain corresponding sets; and processing sampling points in each set according to preset growth criteria to respectively construct to-be-selected building area images, superimposing all the to-be-selected building area images to obtain a final building area image. Through the method or the system provided by the invention, a building area can be quickly and accurately extracted from the Sentinel-1 SAR image, automatic extraction of construction land of a large region is realized, and a foundation is laid for global building area mapping.

The invention discloses oil-marinated food and a preparation method thereof. The oil-marinated food comprises the following raw materials in parts by weight: 1500-2000 parts of raw material meat, 250-420 parts of marinating oil, 50-100 parts of white granulated sugar, 50-100 parts of a chicken essence, 20-40 parts of monosodium glutamate, 50-100 parts of table salt and 100-150 parts of secret spices. By the preparation method, the freshness of a food material can be better locked, and water is continuously evaporated in the marinating process, so that juice separated out from the food materialcan be in a continuous concentrating reaction process, and the taste is richer and thicker.

The invention provides an opopanax extract. The extract is prepared by extracting opopanax by a polar solvent, adsorbing the crude extract by virtue of adsorption resin, and eluting by eluent composed of water and ethanol. The opopanax extract is prepared by using a single raw material opopanax, is simple and effective, is capable of inhibiting the activity of tyrosinase, can be applied to cosmetics, and can be used for producing foods, animal feeds, medicines, quasi-drugs and the like.

The invention discloses a response surface optimized coix seed polyphenol extraction method, and belongs to the technical field of coix seed polyphenol extraction. According to the response surface optimized coix seed polyphenol extraction method, an ultrasonic method is adopted to extract coix seed polyphenol, gallic acid is taken as a standard substance, foline-phenol is taken as a color developing agent, and the content of polyphenol is tested by a colorimetric method. The effects of the seven factors of a solvent type, a solid-liquid ratio, a solvent volume fraction, extraction temperature, extraction time, extraction times and particle size of a sample on the extraction rate of the coix seed polyphenol are investigated by an experiment. On the basis of a single factor experiment, a Box-Behnken response surface analysis method is adopted to design an experiment scheme, the solid-liquid ratio, the solvent volume fraction, the extraction time and the extraction temperature are takenas experimental factors, the extraction rate of the coix seed polyphenol is a response value, a regression model is established, the validity of the regression model and the influence degree of various single factors are analyzed, various factors during the coix seed polyphenols extraction are optimized, and optimized process conditions of the coix seed polyphenol extraction are acquired. A good fitting degree exists between an actually tested value and a theoretical value so that the method is suitable for the coix seed polyphenols extraction.

The invention provides a method for separation and determination of arsenic in PM2.5. According to the method, nitric acid with a concentration of 1% is used as an extraction solvent, and high performance liquid chromatography and inductively coupled plasma mass spectrometer are used in combination for extraction and determination of arsenic forms in PM2.5. The method can completely separate arsenic of four different forms within 10 min, wherein the arsenic forms include trivalent arsenic [As(III)], pentavalent arsenic [As(IV)] monomethyl arsenic (MMA) and dimethyl arsenic (DMA); the detection limit of the method is in a range of 0.6 to 1 [mu]g / L; the precision degrees of As(III), DMA, MMA and As(IV) are 1.26%, 1.24%, 1.00% and 0.79%, respectively; and obvious conversion among the four arsenic forms does not occur during determination.

The invention particularly relates to a kit for quickly extracting double-stranded RNA of a mycovirus and application of the kit. The kit comprises a cell lysis buffer, a nucleic acid adsorption solution, a nucleic acid cleaning solution and a double-stranded RNA agarose gel electrophoresis reagent. According to the invention, a new buffer solution system is adopted, a silicon film is used to replace cellulose powder to be used as an adsorption medium, organic reagents such as phenol are not required, through the reactions of the cell lysis buffer and the nucleic acid adsorption solution, impurities such as proteins, most DNA and polyose are removed in a chemical precipitation manner, and finally, by adjusting adsorption conditions through alcohol, the double-stranded RNA is effectively combined with the silicon film and the purified double-stranded RNA is obtained through elution. Through the adoption of the kit, high-quality double-stranded RNA can be prepared from a hypha sample with the weight as low as 0.3 g, and the whole extraction process is conducted within one hour; steps of organic reagent extraction and supernatant suction in a conventional method are eliminated, and all operations related to column-passing and the like are completed through centrifugation. The kit is suitable for high flux treatment of a large number of samples.

The invention discloses a method for extracting marine animal phospholipids by ultrasonic reflux. The method comprises the following steps: (1) sample pretreatment: homogenating and freeze-drying visceral organs of marine animals, and screening the visceral organs by a screen of 60-80 meshes to prepare freeze-dry powder; (2) ultrasonic reflux extraction: adding a solvent into a liquid storage tank of ultrasonic reflux extraction equipment, heating to be 40-45 DEG C, feeding baits from a material inlet according to a material-to-liquid ratio of 1: 6-1: 10, performing reflux extraction by a spiral propeller, switching on ultrasonic waves, and opening a liquid outlet to enable extracting solution to enter the liquid storage tank; (3) concentration: performing rotary evaporation on the extracting solution to concentrate to a thick material; (4) deoiling: adding acetone into the concentrated thick material for digestion until the solvent is colorless, and performing suction filtration; (5) drying: performing vacuum drying on a filtering cake to obtain the marine animal phospholipids. Compared with the conventional phospholipids extraction method, the method disclosed by the invention has the advantages that the extraction rate and the extraction efficiency are higher, the use amount of ethyl alcohol can be reduced, and energy can be saved; the extraction temperature is reduced, and the active function of a product cannot be lost.

The invention relates to a detection method for the perfuming uniformity of cigarette shreds. The method sequentially comprises the following steps: A. confirming a label of characterization uniformity; B. sampling from an exit of a cigarette shred perfuming roller; C. balancing the cigarette shred sample obtained by sampling; D. pre-treating the cigarette shred sample by extraction with a rapid solvent extraction instrument, and preparing a filtering medium; E. carrying out gas chromatography and mass spectrum combined analysis on the filtering medium, taking the label as the analysis object, and adopting a selected ion scanning pattern; and F. calculating the content of the label in the cigarette shred sample, obtaining a standard deviation (SD), and reflecting the perfuming uniformity degree of the cigarette shred by the SD value. The detection method adopts the gas chromatography / mass spectrum instrument as the separation and analysis means, and quantifying-researches the uniformity of the perfuming procedure of the cigarette shreds, thereby confirming the uniformity of cigarette shred perfuming. The detection method is applied to the perfuming technological process in the cigarette production, thereby providing a practical and scientific method for the detection of the cigarette perfuming precision.

The invention provides Chufeng ointment and an application thereof in treatment of maternal nipple cracking. The Chufeng ointment comprises, by weight, 1 parts of an egg yolk oil extract, 0.5-1.5 parts of sesame oil and 1.5-2.5 parts of pure natural lanolin. The preparation method of the egg yolk oil includes: boiling an egg, peeling the egg to take the yolk, crushing the yolk, performing cold leaching or ultrasonic extraction with food-grade ethyl acetate, and depressing an obtained extracting solution to have ethyl acetate recovered to obtain the egg yolk extract. The Chufeng ointment prepared with the method is applicable to treatment of maternal nipple cracking, and an indigenous method having a long history of medication in traditional Chinese medicine books is displayed in a modern way; the preparation method of the Chufeng ointment is simple in process, the Chufeng ointment is suitable for the special group of mothers and babies at the particular period of the suckling period and free from side effects for the mothers and babies, breast-feeding is unaffected, and safety and effectiveness are achieved.

The embodiment of the invention provides a document information extraction method and a device. The method comprises the steps of determining an area serving as an anchor point area in a to-be-extracted document image, adjusting an image with a to-be-extracted contour to be in the size of a preset template image, obtaining a target image, adjusting the area to be in the size of a preset template anchor point, and obtaining a target anchor point; determining the position of the target anchor point in the target image, and obtaining the position of the region of interest in the target image by combining the pre-defined region of interest of the template image with the relative position of the template anchor point; according to the size of a pre-defined region of interest in the template image, in combination with the position of the region of interest, extracting the region of interest from the target image; wherein the template image is defined according to a document image to be extracted. The embodiment of the invention is particularly suitable for extracting document information in a large number of document images with fixed formats.

The invention provides a method for extracting a food natural pigment from green plum pericarps. The method comprises the following steps: selecting and washing fresh green plums; heating the fresh green plums by adopting microwaves and dissolving into oversaturated table salt; adding a complex enzyme composed of cellulase and pectinase and carrying out enzymolysis; crystallizing and separating out at low temperature by adopting the dissolved table salt; then extracting the pigment by adopting an ultrasonic extraction manner. According to the method provided by the invention, the extraction efficiency and the extraction rate of the pigment are effectively improved and the pigment has extremely high food safety. The problems that a product produced by a traditional natural pigment extraction method has poor quality, low concentration, undesirable odor and residual solvent are overcome.

The invention provides a method for rapidly obtaining drug-resistant genes of probiotics in a sterilized dairy product based on a high-throughput sequencing technology, which comprises the following steps of: extracting DNA (Deoxyribose Nucleic Acid) of the sterilized dairy product, and then analyzing the drug-resistant genes carried by the probiotics in the sterilized dairy product by combining the high-throughput sequencing technology with a bioinformatics method. According to the method for rapidly obtaining the drug-resistant genes of the probiotics in the sterilized dairy product based on the high-throughput sequencing technology, the drug-resistant bacteria genes of the lactic acid bacteria in the sterilized dairy product can be efficiently and rapidly screened, and the method has a good application prospect.

The invention relates to the technical field of biology, and particularly discloses a method for extracting Chinese eaglewood essential oil. The method comprises the steps of crushing a Chinese eaglewood raw material, adding water, and stirring to obtain slurry; processing the slurry by a colloid mill; performing ultrasonic treatment on the slurry and hydrolyzing by using cellulase; performing enzyme inactivation and suction filtration after hydrolysis, extracting the prepared first filtrate by using 95% ethanol, performing suction filtration again on the organic phase, distilling prepared second filtrate for removing ethanol, thus obtaining the Chinese eaglewood essential oil. According to the method disclosed by the invention, the Chinese eaglewood essential oil is subjected to double-effect extraction by using ultrasonic waves and an enzymolysis method, so that the extraction rate of the Chinese eaglewood essential oil is effectively increased to ensure that the Chinese eaglewood essential oil extraction rate of the Chinese eaglewood raw material containing a whitewood layer is increased up to be more than 0.4%, and meanwhile, the extraction time is shortened; the method is suitable for extracting the Chinese eaglewood essential oil of natural Chinese eaglewood and artificial Chinese eaglewood.

The invention particularly relates to a kit for quickly extracting double-stranded RNA of a mycovirus and application of the kit. The kit comprises a cell lysis buffer, a nucleic acid adsorption solution, a nucleic acid cleaning solution and a double-stranded RNA agarose gel electrophoresis reagent. According to the invention, a new buffer solution system is adopted, a silicon film is used to replace cellulose powder to be used as an adsorption medium, organic reagents such as phenol are not required, through the reactions of the cell lysis buffer and the nucleic acid adsorption solution, impurities such as proteins, most DNA and polyose are removed in a chemical precipitation manner, and finally, by adjusting adsorption conditions through alcohol, the double-stranded RNA is effectively combined with the silicon film and the purified double-stranded RNA is obtained through elution. Through the adoption of the kit, high-quality double-stranded RNA can be prepared from a hypha sample with the weight as low as 0.3 g, and the whole extraction process is conducted within one hour; steps of organic reagent extraction and supernatant suction in a conventional method are eliminated, and all operations related to column-passing and the like are completed through centrifugation. The kit is suitable for high flux treatment of a large number of samples.

The invention relates to a technology for extracting effective ingredients from natural products and extracting resveratrol from mulberries. The technology comprises the steps that the mulberries are dried and smashed, and resveratrol is extracted through ultrasonic waves and purified to obtain the resveratrol. According to the technical scheme of the method, dual solvent and ultrasonic extraction and integration are adopted, the characteristic that the solubleness of the resveratrol in the mulberries is higher in weak polar solvent is utilized, and an ultrasonic extraction technology is utilized, so that the technology has the advantages of being high in extraction efficiency and product purity and low in extraction cost and energy consumption; the solvent used in the extraction process can be recycled and little in usage amount, so that the influence to the environment is lower, and the biological activity of the resveratrol is prevented from being destroyed in the extraction process.

The invention relates to a technique for extracting effective ingredients from a natural product, particularly a technique for extracting resveratrol and pigment from grape skin, which comprises the following steps: drying the grape skin, pulverizing, extracting resveratrol by ultrasonic waves, extracting pigment by ultrasonic waves, and purifying to obtain the resveratrol product and the pigment product. In the technical scheme provided by the invention, double solvents and ultrasonic extraction are integrated; the technical scheme provided by the invention has the advantages of high extraction efficiency, low extraction cost and low energy consumption by using the ultrasonic extraction technique according to the principle that the resveratrol and pigment in the grape skin have different solubilities in solvents with different polarities; the solvents used in the extraction process can be utilized cyclically, so that the solvent consumption is low, and the environmental influence is low, thereby avoiding destroying the bioactivities of the resveratrol and the pigment in the extraction process; and thus, the invention is suitable for extracting bioactive substances.

The invention discloses a resume information extraction method and system based on deep learning. The method comprises the following steps: collecting Chinese corpora of resumes, and taking the Chinese corpora as training data; constructing a neural network model according to information of the Chinese corpora; training the constructed neural network model through the training data until convergence; and inputting resumes into the neural network for information extraction. The method and the system have the beneficial effects that complex semi-structured data in resumes can be well dealt with when the method provided by the invention is used for extracting resume information; due to the fact that deep learning has strong characterization capabilities, features can be well extracted; and meanwhile, the method also has good generalization ability and is suitable for information extraction.

The response surface optimization method for extracting barley polyphenols belongs to the technical field of barley polyphenol extraction. The invention adopts an ultrasonic method to extract coix seed polyphenols, uses gallic acid as a standard product and folinol as a color developing agent, and measures the polyphenol content by a colorimetric method. The experiment investigated the effects of seven factors including solvent type, solid-liquid ratio, solvent volume fraction, extraction temperature, extraction time, extraction times, and sample particle size on the extraction rate of barley polyphenols. On the basis of the single factor experiment, the experimental scheme was designed by using the Box-Behnken response surface analysis method, and the material-liquid ratio, solvent volume fraction, extraction time, and extraction temperature were used as the experimental factors. The effectiveness of the regression model and the influence of each single factor were analyzed, and the factors in the extraction of polyphenols from the seed of Job's tears were optimized to obtain the optimized process conditions for the extraction of polyphenols from the seed of Job's tears. There is a good fit between the measured value and the theoretical value, which is suitable for the extraction of polyphenols from barley.

The invention discloses a method for quickly extracting low molecular weight RNA of plant. After cells are disrupted, genomic DNA and high molecular weight RNA form a DNA-protein complex and a RNA-protein complex with protein respectively; and the DNA-protein complex and the RNA-protein complex are insoluble in acid and low-salt environment, and can be removed and extracted through acid and phenol. The low molecular weight RNA stays in supernatant solution, and can be recovered through the precipitation of ethanol or isopropanol. The method is quick and efficient, and can recover the low molecular weight RNA only by one-step precipitation after phenol extraction. The low molecular weight RNA extracted by the method has high purity, and can be used for the cloning of the low molecular weight RNA and the detection of Northern blot hybridization. The method has simple and quick experimental process, and is suitable for the extraction of the low molecular weight RNA of various plant tissues.

The invention provides a method for extracting high-purity marine phospholipids from Euphausia superba; the method comprises the steps of homogenizing Euphausia superba, and enzymatically hydrolyzing with enzyme complex; carrying out solid-liquid separation, adding the solid portion to alkaline ethanol solution, and carrying out ultrasonic-assisted extraction; water-precipitating and deoiling, and carrying out polystyrene gel column chromatography; washing with glacial acetic acid solution to obtain high-purity phospholipids. The materials herein are enzymatically hydrolyzed with the enzyme complex of bromelain and chitinase, efficient extraction is carried out by using the alkaline ethanol solution, phospholipids extraction efficiency is improved, whole-course temperature is not higher than 50 DEG C, and biophysical activity of phospholipids is not higher than 95% which is significantly higher than that of the prior art.