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A method for extracting high-purity marine phospholipids from Antarctic krill

An Antarctic krill, high-purity technology, applied in the direction of edible phospholipid composition, chemical instruments and methods, compounds of group 5/15 elements of the periodic table, etc., can solve the problems of robbing food resources, affecting the quality of phospholipids, and high operating costs , to achieve the effects of good mechanical strength of the filler, easy operation and high extraction efficiency

Active Publication Date: 2018-06-29
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are many technical defects in these methods: raw materials are mainly extracted from soybeans and egg yolks, thereby robbing humans of food resources; supercritical extraction methods, membrane separation methods and other processes are relatively complicated, and the operation cost is high; inorganic salt precipitation method introduces metal Ions (Zn 2+ 、Cd 2+ etc.), have certain toxicity, affect the quality of phospholipids, etc.

Method used

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  • A method for extracting high-purity marine phospholipids from Antarctic krill
  • A method for extracting high-purity marine phospholipids from Antarctic krill
  • A method for extracting high-purity marine phospholipids from Antarctic krill

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Weigh 100g of cryopreserved Antarctic krill, add 50mL of water, pulverize and homogenize for 18min, adjust the pH to 6.5 with acetic acid-sodium acetate buffer solution, heat to 40°C, add 0.5g of compound enzyme, and hydrolyze for about 3h; Centrifuge the solid part through 1000mL of 93% ethanol solution (sodium acetate adjusts its pH to 9.0), ultrasonically extract twice at 30°C, each time for 30min, and the ultrasonic frequency is 30KHz, combine the extracts, and filter under reduced pressure. Concentrate under reduced pressure at 45°C to obtain the extract concentrate; stir the extract concentrate at a speed of 60 revolutions per minute, heat the temperature to 35°C, reduce the stirring speed to 25 revolutions per minute, slowly and evenly add distilled water, the amount of water is 3% of oil weight, water temperature 30°C, water mixing time is 15min, after mixing water, the stirring speed is restored to 60 rpm, stop stirring when the flocs precipitate, let stand for ...

Embodiment 2

[0040] Weigh 100g of fresh Antarctic krill, add 100mL of water, pulverize and homogenize for 12min, adjust the pH to 6.5 with acetic acid-sodium acetate buffer solution, heat to 45°C, add 0.7g of compound enzyme, and hydrolyze for about 4h; centrifuge the enzymatic solution, The solid part was subjected to 1200mL of 95% ethanol solution (sodium bicarbonate to adjust its pH to 9.0), ultrasonically extracted twice at room temperature, each time for 15min, and the ultrasonic frequency was 30KHz, the combined extracts were filtered under reduced pressure, and the filtrate was reduced by 45°C. Concentrate under pressure to obtain the extract concentrate; stir the extract concentrate at a speed of 60 revolutions per minute, heat the temperature to 35°C, reduce the stirring speed to 25 revolutions per minute, and slowly and evenly mix in distilled water, the amount of water added is the weight of the oil 3%, the water temperature is 30°C, and the water mixing time is 15 minutes. After...

Embodiment 3

[0042] Weigh 100g of fresh Antarctic krill, add 200mL of water, pulverize and homogenize for 8 minutes, adjust the pH to 6.5 with acetic acid-sodium acetate buffer solution, heat to 43°C, add 0.8g of compound enzyme, and hydrolyze for about 2 hours; the enzymatic solution is separated from solid and liquid , the solid part was subjected to 600mL of 90% ethanol solution (sodium bicarbonate adjusted its pH to 10.0), ultrasonically extracted twice at 35°C, each time for 20min, and the ultrasonic frequency was 40KHz, the combined extracts were filtered under reduced pressure, and the filtrate was subjected to 43 Concentrate under reduced pressure at ℃ to obtain the extract concentrate; stir the extract concentrate at a speed of 60 rpm, heat the temperature to 35°C, reduce the stirring speed to 25 rpm, slowly and evenly mix in distilled water, the amount of water mixed is oil 3% by weight, water temperature 30°C, water mixing time 15min, after mixing water, the stirring speed is res...

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Abstract

The invention provides a method for extracting high-purity marine phospholipids from Euphausia superba; the method comprises the steps of homogenizing Euphausia superba, and enzymatically hydrolyzing with enzyme complex; carrying out solid-liquid separation, adding the solid portion to alkaline ethanol solution, and carrying out ultrasonic-assisted extraction; water-precipitating and deoiling, and carrying out polystyrene gel column chromatography; washing with glacial acetic acid solution to obtain high-purity phospholipids. The materials herein are enzymatically hydrolyzed with the enzyme complex of bromelain and chitinase, efficient extraction is carried out by using the alkaline ethanol solution, phospholipids extraction efficiency is improved, whole-course temperature is not higher than 50 DEG C, and biophysical activity of phospholipids is not higher than 95% which is significantly higher than that of the prior art.

Description

technical field [0001] The invention relates to a method for extracting marine phospholipids from Antarctic krill, and belongs to the technical field of marine active substances. Background technique [0002] Antarctic krill is a kind of crustacean zooplankton living in the Southern Ocean. It is one of the largest single species of biological resources on the earth. The catchable amount can reach 130 million tons, making it a huge potential fishery resource. At present, my country has listed Antarctic krill as one of the main development species for the development of distant water fishery in the future. Antarctic krill is rich in phospholipids, and the fatty acid composition of phospholipids is mainly EPA and DHA, which account for more than 40% of the total fatty acids in phospholipids. Therefore, the preparation of high-purity phospholipids in Antarctic krill must be one of the most critical technologies in the deep processing of Antarctic krill. [0003] Phospholipids...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10
CPCC07F9/103
Inventor 刘可春李晓彬韩利文何秋霞楚杰王荣春陈锡强张轩铭王莹靳梦
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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