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Method for extracting marine animal phospholipids by ultrasonic reflux

A countercurrent extraction and marine animal technology, applied in the direction of edible phospholipid composition, application, food science, etc., can solve the problems of unfavorable preparation of phospholipids, high investment cost, reagent residue, etc., achieve high extraction efficiency, save energy, and low extraction temperature Effect

Inactive Publication Date: 2015-04-29
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction methods of phospholipids mainly include: solvent method, supercritical CO 2 Extraction, column chromatography, membrane separation, etc., but there are many problems in the above methods, such as reagent residue, high investment cost, low production capacity, etc., which are not conducive to the preparation of phospholipids with active functions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting marine animal phospholipids by ultrasonic countercurrent, the specific steps are as follows:

[0024] (1) Homogenize 10 kg of marine animal viscera, freeze-dry, and pass through a 60-mesh sieve to obtain freeze-dried powder;

[0025] (2) Ultrasonic countercurrent extraction: add ethanol to the liquid storage tank of the TGCXN energy-concentrating ultrasonic countercurrent extraction machine, and heat it to 40°C; start feeding from the feeding port, and feed the material according to the ratio of material to liquid at 1:6, and carry out countercurrent flow through the screw propeller Extract and turn on the ultrasonic wave (parameters: frequency 20 kHz, power 1000 W, time 40 min); open the liquid outlet to allow the extraction liquid to enter the storage tank;

[0026] (3) Concentration: collect the extract and concentrate it to a thick substance by rotary evaporation;

[0027] (4) Deoiling: The concentrated thick matter is extracted with cold ac...

Embodiment 2

[0030] A method for extracting marine animal phospholipids by ultrasonic countercurrent, the specific steps are as follows:

[0031] (1) Sample pretreatment: Take 8kg of marine animal organs and homogenate, freeze-dry, and pass through a 80-mesh sieve to obtain freeze-dried powder;

[0032] (2) Ultrasonic countercurrent extraction: add ethanol to the liquid storage tank of the TGCXN energy-concentrating ultrasonic countercurrent extraction machine, and heat it to 45°C; start feeding from the feeding port, and feed according to the ratio of material to liquid at 1:9, and carry out countercurrent flow through the screw propeller Extract and turn on the ultrasonic wave (parameters: frequency 25KHz, power 1300W, time 45min); open the liquid outlet to allow the extraction liquid to enter the storage tank;

[0033] (3) Concentration: collect the extract and concentrate it to a thick substance by rotary evaporation;

[0034] (4) Deoiling: The concentrated thick matter is extracted w...

Embodiment 3

[0037] A method for extracting marine animal phospholipids by ultrasonic countercurrent, the specific steps are as follows:

[0038] (1) Sample pretreatment: 12kg of marine animal viscera was homogenized, freeze-dried, and passed through a 70-mesh sieve to obtain a freeze-dried powder;

[0039] (2) Ultrasonic countercurrent extraction: add ethanol to the liquid storage tank of the TGCXN energy-concentrating circulating ultrasonic extraction machine, and heat it to 42°C; start feeding from the feeding port, and feed according to the material-to-liquid ratio of 1:9, and carry out countercurrent flow through the screw propeller Extract and turn on the ultrasonic wave (parameters: frequency 30KHz, power 1500W, time 50min); open the liquid outlet to allow the extraction liquid to enter the storage tank;

[0040] (3) Concentration: collect the extract and concentrate it to a thick substance by rotary evaporation;

[0041] (4) Deoiling: The concentrated thick matter is extracted wit...

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Abstract

The invention discloses a method for extracting marine animal phospholipids by ultrasonic reflux. The method comprises the following steps: (1) sample pretreatment: homogenating and freeze-drying visceral organs of marine animals, and screening the visceral organs by a screen of 60-80 meshes to prepare freeze-dry powder; (2) ultrasonic reflux extraction: adding a solvent into a liquid storage tank of ultrasonic reflux extraction equipment, heating to be 40-45 DEG C, feeding baits from a material inlet according to a material-to-liquid ratio of 1: 6-1: 10, performing reflux extraction by a spiral propeller, switching on ultrasonic waves, and opening a liquid outlet to enable extracting solution to enter the liquid storage tank; (3) concentration: performing rotary evaporation on the extracting solution to concentrate to a thick material; (4) deoiling: adding acetone into the concentrated thick material for digestion until the solvent is colorless, and performing suction filtration; (5) drying: performing vacuum drying on a filtering cake to obtain the marine animal phospholipids. Compared with the conventional phospholipids extraction method, the method disclosed by the invention has the advantages that the extraction rate and the extraction efficiency are higher, the use amount of ethyl alcohol can be reduced, and energy can be saved; the extraction temperature is reduced, and the active function of a product cannot be lost.

Description

technical field [0001] The invention belongs to a method for extracting phospholipids from marine animal tissues, in particular to a method for extracting phospholipids from marine animals by means of ultrasonic countercurrent. Background technique [0002] Phospholipids refer to polar lipid compounds containing phosphate radicals, including glycerophospholipids and sphingomyelins, which are the lipid bilayers that constitute biological membranes and are also important components of biological cells. They can maintain cell stability and protect biological cells. It plays an important role in the normal performance of various physiological functions of the body. [0003] Phospholipids exist in various organs such as the liver, brain and heart of animals, as well as in various microorganisms, poultry eggs and seeds of most plants. Currently commercially available phospholipids are mainly derived from soybeans and egg yolks. Studies have found that when phospholipids contain ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10
Inventor 梁鹏许艳萍陈丽娇程文健罗联钰柯范生程新伟
Owner FUJIAN AGRI & FORESTRY UNIV
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