60 results about "Glycerophospholipids" patented technology

Disclosed herein are alternative, enhanced, and cheaper methods of improving cognitive functions in a subject using a lipid composition conjugated with omega-3 and omega-6 fatty acids, with specific amounts and specific conjugation patterns of LA, linolenic acid (alpha-linolenic acid, gamma-linolenic acid) DHA and eicosapentaenoyl (EPA), e.g. utilizing different sources of lipids.

Disclosed herein is a lipid preparation, said preparation comprising a phosphatidylserine moiety, and poly-unsaturated fatty acid (PUFA) acyl groups, particularly long-chain poly-unsaturated fatty acid (LC-PUFA) acyl groups such as omega-3 and/or omega-6 acyl groups, wherein said PUFA is covalently bound to said glycerophospholipid. Said lipid preparations are particularly useful in the treatment of mental and cognitive disorders, e.g. ADHD (attention deficit hyperactivity disorder) and Alzheimer's disease.

The disclosed preparations present improved bioactivity, and are useful in the treatment of various cognitive and mental conditions and disorders, as well as for maintenance of normal functions of brain-related systems and processes.

A stable polymer micella carrier system for medicines is composed of an amphipathic block copolymer including diblock copolymer and triblock copolymer and a phosphatide chosen from glyceryl phosphatide, sphingomyelin, and their derivatives.

The present invention features glycerophospholipids having a single fatty acid moiety and the use thereof in darkening the skin.

A new enzymatic process for preparing 1,2-diacylated phospholipids using an enzyme preparation possessing phospholipase activity towards acylation at the sn-1 and sn-2 sites in a microaqueous reaction system. More particularly, the 1,2-diacyl-phospholipids produced according to the esterification/transesterification process are obtainable in high yield and purity and carry identical desired carboxylic acid, preferably fatty acid, acyl groups at the sn-1 and sn-2 positions. The process involves esterification/transesterification (acylation) of a glycerophospholipid, preferably glycerophosphoryl choline (GPC) with a desired carboxylic acid, preferably fatty acid, or their derivatives in the presence of the above mentioned appropriate enzyme preparation. The process of the invention further relates to a process for the production of 1-acyl-2-lyso-glycerophospholipid, preferably 2-lyso-PC by reacting glycerophospholipid, preferably glycerophosphoryl choline (GPC) with a desired carboxylic acid, preferably fatty acid, or their derivatives in the presence of a sn-1 specific phospholipase (PLA₁ or PLA_1,2) and a solvent, in a microaqueous medium.

The invention discloses a method for preparing natural L-alpha-glyceryl phosphatide dipalmitoyl phosphatidyl choline. The method comprises the following steps of: dissolving natural lecithin having the known content of phosphatidylcholine into methyl tertiary butyl ether; filtering out undissolved substances and regulating the temperature to 5-55 DEG C; adding anhydrous low carbon alcohol and hydroxide choline into mixing liquid in a molar rate of anhydrous low carbon alcohol to phosphatidylcholine of (3:1)-(600:1)_to stir and react for 0.25-4 hours; pouring out supernate; dissolving oily precipitates with absolute ethyl alcohol and precipitating the oily precipitates by methyl tertiary butyl ether; removing the supernate after the precipitation is totally finished; repeatedly dissolving the precipitates by the absolute ethyl alcohol and precipitating the precipitates by the methyl tertiary butyl ether for 2-3 times; vacuumizing and drying obtained oily products. By the method, transesterification between the phosphatidylcholine and the anhydrous low carbon alcohol is carried out by using hydroxide choline as a catalyst and the methyl tertiary butyl ether as a solvent so as to obtain glycerol phosphatidylcholine (GPC) or natural lecithin deacylation substances using the GPC as a main part; and moreover, the method is simple in process, mild and safe in reaction conditions, and can guarantee the safety of the obtained products used as functional food addictives or medical health-care products.

Disclosed is a lipid preparation comprising a glycerophospholipid or salt, conjugate and derivatives thereof, particularly phosphatidylserine (PS), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidyl-inositol (PI), phosphatidylglycerol (PG) and phosphatidic acid (PA), and poly-unsaturated fatty acid (PUFA) acyl groups, particularly long-chain poly-unsaturated fatty acid (LC-PUFA) acyl groups such as omega-3 and/or omega-6 acyl groups, wherein said PUFA is covalently bound to said glycerophospholipid. The disclosed preparations possess an improved bioactivity, and are useful in the treatment of various cognitive and mental conditions and disorders and for maintenance of normal functions of brain-related systems and processes, for example ADHD.

Disclosed is a process for producing sphingomyelin and plasmalogen-form glycerophospholipid, which comprises the step (A) of extracting a total lipids from a chicken skin powder and drying the extract, the step (B) of subjecting the dried total lipids obtained in said step (A), to extraction treatment with a solvent mixture of an aliphatic hydrocarbon solvent and a water-soluble ketone solvent to separate an insoluble portion composed mainly of sphingomyelin and a soluble portion, the step (C) subjecting the insoluble portion composed mainly of sphingomyelin, obtained in said step (B), to extraction treatment with a solvent mixture of water and a water-soluble ketone solvent to remove a non-lipid component contained in the soluble portion, and the step (D) of drying the soluble portion obtained in said step (B), and subjecting the thus-obtained dried product to extraction treatment with a water-soluble ketone solvent to separate and recover an insoluble portion composed mainly of plasmalogen-form giycerophospholiuld.

According to the above production process, high-purity sphingomyelin, in particular, human-form sphingomyelin and plasmalogen-form sphingomyelin can be produced from chicken skin at high yields with simple procedures.

Peptide-lipid constructs of the structure L-S—F are disclosed, where F is a peptide, S is a spacer covalently linking F to L via an oligomer of ethylene glycol, and L is a diacyl- or dialkyl-glycerolipid (including glycerophospholipids). The spacer ideally has 6 to 14 ethylene glycol repeats, corresponding to PEG with a molecular weight of approximately 250 to 600. Also disclosed is a method of detecting reactive antibodies in serum by contacting serum with cells modified to incorporate a peptide-lipid construct, where the peptide is an epitope of the antibody, and determining the degree of aggluthination of the cells.

Systems, devices, and methods for delivering one or more active ingredients to intradermal tissues, deep regions of pores, and intradermal tissues in the vicinity of pores. In some embodiments, a composition is provided including a plurality of liposomes including a cationic lipid, and an amphiphilic glycerophospholipid having a saturated fatty acid moiety and an unsaturated fatty acid moiety, and at least one insulin, insulin analog, or insulin derivative.

This invention provides a process for producing a high-purity sphingomyelin and a high-purity plasmalogen-form glycerophospholipid from a biological material by simple procedures at high yields. The process comprises the steps of: (A) subjecting dried total lipids extracted from a biological material to extraction treatment with a specific mixture solution to separate an insoluble portion composed mainly of sphingomyelin and a soluble portion; (B) subjecting the insoluble portion, obtained in said Step (A), to washing treatment with a specific mixture solution to obtain crude sphingomyelin; (C) subjecting the soluble portion, obtained in said Step (A), to washing treatment with a water-soluble ketone solvent to obtain crude plasmalogen-form glycerophospholipid; (D) causing an enzyme to act on the crude sphingomyelin, obtained in said Step (B), to obtain sphingomyelin having a purity of 90% or more; and (E) causing an enzyme to act on the crude plasmalogen-form glycerophospholipid, obtained in said Step (C), to obtain plasmalogen-form glycerophospholipid having a purity of 40% or more.

The invention discloses a preparation method of a porphyrin/carbon nitride layer-by-layer composite structure photocatalytic nano-composite material. The preparation method comprises the following steps: taking and dissolving melamine and L-alpha glycerylphosphorylcholine in deionized water, carrying out a hydrothermal reaction in a hydrothermal kettle, centrifuging the obtained solution, and washing and drying the obtained reaction product to obtain a solid; adding KOH and a carbon fiber into the solid, performing mixing, putting the obtained mixture into a crucible, putting the crucible intothe resonant cavity of a microwave oven, and performing vacuum heating to obtain a heated product; cooling the heated product to room temperature, and washing, drying and grinding the cooled heated product to obtain a carbon nitride powder; dissolving the carbon nitride powder into anhydrous ethanol to obtain a solution A; adding TCPP into anhydrous ethanol to prepare a solution B; adding the solution B into the solution A to obtain a mixed solution C; and continuously stirring the mixed solution C until the solvent is completely volatilized, and performing drying to obtain the porphyrin/carbon nitride layer-by-layer composite structure photocatalytic nano-composite material. The specific surface area of carbon nitride can be increased, the charge transmission and separation efficiency ofcarbon nitride is improved, and absorption and utilization of carbon nitride on visible light are improved.

A method for analyzing a sample that contains a plurality of lipid isomers is described that involves forming one or more lipid metal ion adducts and transporting the one or more lipid metal ion adducts through a Mix of POPC+OPPC differential mobility spectrometer to cause separation of the one or more lipid metal ion adducts from each other. The lipid isomers can be chosen, for example, from fatty acids, glycerolipids, glycerophospholipids, sphingolipids, saccharolipids, polyketides, sterol lipids, and prenol lipids. Particular examples include phosphatidylcholine regioisomers such as 1-palmatoyl-2-oleoyl-sn-phosphatidylcholine (POPC) and 1-oleoyl-2-palmatoyl-sn-phosphatidylcholine and triacylglycerols containg palmetic and oleic acid groups. The metal chosen can include a cationization reagent that contains sodium, potassium, silver or lithium.

The invention discloses a synthetic method of a nitrogen mustard-glycerol phosphatidyl choline compound. The synthetic method comprises the following step of reacting at room temperature by taking a nitrogen mustard-type compound and glycerol phosphatidyl choline as raw materials, taking N, N'-carbonyldimidazole and 1, 8-diazabicyclo C11-7-alkene and taking an aprotic solvent as a reaction solvent, thereby preparing the nitrogen mustard-glycerol phosphatidyl choline compound. The method disclosed by the invention is simple to operate, is mild in reaction condition, and is high in yield.

[PROBLEM TO BE SOLVED] To provide a method for producing ether phospholipids of high purity by an easy process. [SOLUTION] This production method comprises: (A) a step of extracting total lipids from organisms with non-polar organic solvent and branched alcohol mixture; and (B) a step of reacting the total lipids obtained by the step (A) with phospholipase A1 to decompose mixed diacyl-glycerophospholipids. This makes it possible to produce plasmalogen-type glycerophospholipids or ether phospholipids of high purity by an easy process.

An object of the present invention is to provide a technique for masking the bitterness of collagen peptide containing compositions.

By incorporating glycerophospholipids comprising neutral glycerophospholipids and acidic glycerophospholipids in specified proportions or incorporating sphingoglycolipids in a specified proportion relative to collagen peptides, there can be provided methods for masking the bitterness of collagen peptide containing compositions.

The invention relates to the use of phospholipases, v.gr. sPLA₂, as an active agent in the production of pharmaceutical and/or cosmetic compositions for the treatment of diseases caused by micro-organisms, the envelope or membrane of which comprises phospholipids, particularly glycerophospholipids. In particular, said invention relates to the use of sPLA₂ from Crotalus durissus terrificus venom as a biocide component for the preparation of compositions for the treatment of viral infections, particularly acquired immunodeficiency states VIH-1 and VIH-2, bacterial and parasitic infections, such as infections caused by plasmodium and leishmania-type parasites.

The invention provides a method of preparing L-alpha-glycerol phosphatidylcholine. The method includes the steps of: 1) performing a reaction to halogenated glycerol and ester under catalysis by an acid to produce a halogenated glycerol double-hydroxyl-protected substance I; 2) performing a reaction to phosphatidylcholine salt with the halogenated glycerol double-hydroxyl-protected substance I toobtain an L-alpha-glycerol phosphatidylcholine double-hydroxyl-protected substance II crude product; 3) separating the L-alpha-glycerol phosphatidylcholine double-hydroxyl-protected substance II fromthe non-reacted halogenated glycerol double-hydroxyl-protected substance I to obtain pure L-alpha-glycerol phosphatidylcholine double-hydroxyl-protected substance II; 4) performing deprotective reaction under catalysis by acid in catalytic amount to the pure L-alpha-glycerol phosphatidylcholine double-hydroxyl-protected substance II to obtain a water solution of the L-alpha-glycerol phosphatidylcholine, and performing pressure-reduced evaporation to remove water and performing crystallization with an alcohol solvent to obtain the L-alpha-glycerol phosphatidylcholine. The method, compared withthe prior art, is high in purity and yield and is suitable for industrial production.

The invention discloses a preparation method of carbon nitride with a two-dimensional thin-layer structure. The preparation method comprises the following steps: dissolving melamine and glycerophosphatidylcholine in deionized water and carrying out hydrothermal reaction in a hydrothermal kettle to obtain a mixture solution; centrifuging, washing and drying the mixture solution to obtain a solid; adding KOH into the solid to obtain a solid mixture; taking carbon fibers according to the mass ratio (0.01-0.1): 1 of the carbon fibers to the solid matter, mixing the carbon fibers with the solid mixture to be put into a crucible, putting the crucible into the resonant cavity of a microwave oven for vacuum heating and cooling to room temperature to obtain a heating product; and washing and dryingthe heated product to obtain the carbon nitride with the two-dimensional thin-layer structure. The stripping yield is high, the consumed time is short and the pollution problem in the stripping process can be solved.