32 results about "Lyso-" patented technology

The invention discloses a preserving fluid for stably preserving sample DNA at normal temperature and a preparation method thereof, and belongs to the technical field of biological sample preservation. The preserving fluid comprises a lysis system, a nucleic acid protection system and a buffering and stabilizing system, the cracking system comprises a composite surfactant and an enzymolysis auxiliary agent; the composite surface active agent is prepared from polyether polyol fatty acid ester and cocamidopropyl hydroxy sulfobetaine; the enzymolysis auxiliary agent comprises lysozyme Lyso-V and protease K; the nucleic acid protection system comprises a nitrogen heterocyclic polyamine-carboxylic acid derivative and dextran sulfate; the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N' ', N ''tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N''-triacetic acid, disodium ethylene diamine tetraacetate-nitrogen heterocyclic derivative, and diethylenetriamine pentaacetic acid-piperazine derivative, and the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N '', N'' tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N ''-triacetic acid. The buffering and stabilizing system comprises an amphoteric buffering agent and a polymer stabilizer; the amphoteric buffering agent comprises 2-(N-morpholino) ethanesulfonic acid and N-tri (hydroxymethyl) methylglycine.

The invention discloses a method for determining related substances in a liposome adjuvant and application of the method, and relates to the field of medicine detection. The method is a high performance liquid chromatography, and a mobile phase A comprises an aqueous solution containing trifluoroacetic acid; the mobile phase A comprises a mixed solution of methyl alcohol containing trifluoroacetic acid and acetonitrile, the mobile phase B comprises a mixed solution of methyl alcohol containing trifluoroacetic acid and acetonitrile, simultaneous detection of multiple target substances including oleic acid, ketocholesterol, two isomers of Lyso-DOTAP and two isomers of Lyso-DOPC in the liposome adjuvant is achieved through a specific elution procedure, and the method is good in specificity, high in sensitivity, good in separation degree and high in detection sensitivity. And a new way is provided for evaluating the effectiveness, safety and stability of the liposome adjuvant.

The invention discloses a tellurium-doped carbon quantum dot and a preparation method and application thereof, the tellurium-doped carbon quantum dot is obtained by using 2, 2 '-diaminodiphenyl telluride as a precursor, the average particle size is 1.9 nm, the thickness is about 2.1 nm, and the surface is rich in amino groups. And fixing (4-carboxybutyl) triphenylphosphonium bromide, 3-morpholinopropionic acid or 3-((4-methylphenyl) sulfonamide) propionic acid on the surfaces of the tellurium-doped carbon quantum dots to prepare Mito-Te-CDs, Lyso-Te-CDs and ER-Te-CDs with an organelle targeting function. The Mito-Te-CDs, the Lyso-Te-CDs and the ER-Te-CDs are good in biocompatibility, can be used as fluorescent probes for targeted imaging of organelles, can be used for recognition and removal of superoxide anions in specific organelles, have a protection effect on cells and can be used for effectively treating acute kidney injury.

The invention provides an L-lysine production strain as well as a strain construction method and application. A hom (V59A) is introduced on a genome by utilizing a homologous recombination gene editing technology to weaken a competitive approach of lysine; the lysC (T311I) which is not inhibited by L-lysine is overexpressed; the method comprises the following steps of: overexpressing lysE, lysA, ddh, dapB and asd, knocking out a lysI gene and multi-copying the lysE, carrying out synchronous multi-copying on the dapA and EcdapB genes through a manual operon P1676-EcdapB-Pa12-dapA, and carrying out synchronous multi-copying on the dapB, ddh, lysA and lysO genes through a manual operon Psod-dapB-ddh-Ptuf-lysO-lysA; the constructed strain does not contain plasmids, has no defects, does not need induction, has the advantages of good genetic stability, high fermentation yield and the like, and can be used for stably producing L-lysine.

The invention belongs to the field of biomedicine, and discloses a method and a kit for monitoring a lysosome acidification process mediated by mitochondrial-lysosome interaction. According to the method, an LYSO-PZ probe with a specific structure is used for marking cell lysosome contents, a pH rodo dye and a lysosome membrane marker sfGFP-LAMP1-mCherry are combined for monitoring the pH value and membrane positioning of the lysosome respectively, then a three-channel fluorescence image is obtained through a structural illumination microscope (SIM), and the fluorescence intensity ratio is quantitatively analyzed. In addition, the method further comprises a step of forming mitochondrial-lysosome contact (MLC) through optogenetics regulation, related protein binding is induced through blue light, fluorescence intensity changes before and after MLC formation are compared, and MLC-mediated proton transport is verified. A complete monitoring system is established for the first time, lysosome acidification can be accurately monitored, information can be obtained in multiple dimensions, and the method has important clinical application value and wide market prospects.

The application belongs to the field of biomedicine, and discloses a method and a kit for monitoring a process of mitochondrion-lysosome interaction-mediated lysosome acidification. The method is characterized in that a cell lysosome content is first labeled with a LYSO-PZ probe with a specific structure, a pHrodo dye and a lysosome membrane marker sfGFP-LAMP1-mCherry are combined to monitor the pH value and membrane localization of the lysosome, respectively, then three-channel fluorescence images are acquired through a structured illumination microscope (SIM), and the fluorescence intensity ratio is quantitatively analyzed. In addition, the method further comprises a step of optogenetic regulation of mitochondrion-lysosome contact (MLC) formation, the fluorescence intensity change before and after the formation of MLC is compared through blue light induction of related protein binding, and the proton transport mediated by MLC is verified. The application first establishes a complete monitoring system, can accurately monitor lysosome acidification, and has important clinical application value and broad market prospect.

The invention discloses a high-load transparent rare earth complex organic glass scintillator as well as a preparation method and application thereof. The scintillator comprises a polymer matrix and an ionic rare earth complex dispersed in the polymer matrix, the rare earth complex comprises coordination anions and ionic liquid cations, the coordination anions comprise rare earth ions and organic ligands, and the loading capacity of the rare earth complex in the polymer matrix can reach 80-90 wt%. According to the scintillator, an ionic liquid is introduced to construct an ionic supramolecular structure, effective isolation of a luminescence center is realized by utilizing steric hindrance and a charge shielding effect, concentration quenching is inhibited, and compatibility with a matrix is enhanced, so that the material still keeps transparency of 90% or above under an ultrahigh load. The maximum radiation luminous intensity of the scintillator is 23.9 times that of commercial LYSO, the lower detection limit is as low as 56nGy / s, and the scintillator is suitable for the fields of high-energy particle detection, X-ray imaging and the like.

The invention discloses a synthesis method of a DOTAP (3, 4-Dimethyl-1, 3-pentanedione) dissolved blood lipid. Specifically, the invention provides a synthetic method of a compound as shown in a formula I. The synthetic method is characterized by comprising the following steps: 1, in an organic solvent and in the presence of alkali and a fluorine source, carrying out deprotection reaction on a compound as shown in a formula A4, carrying out quenching reaction by using solid alkali, and recrystallizing to obtain the compound as shown in the formula I; the fluorine source is hydrogen fluoride or hydrogen fluoride salt; the recrystallization solvent is a ketone solvent and an alcohol solvent. The synthetic method disclosed by the invention has relatively high yield and purity, and the conversion from the Lyso-DOTAP-1 to the Lyso-DOTAP-2 in the synthetic process is avoided to a great extent.

Provided are compositions and methods for reducing pre-existing antibodies against viral vector or gene-editing related proteins. The compositions showed reduction in various antibody titers by administration of a liposome composition complexed with a protein or fragment thereof of a viral vector. The liposomes comprise phosphatidylcholine and phosphatidylserine, wherein some or all of the PS is present as lyso-PS. The compositions and methods can be used in conjunction with gene therapy and nucleic acid based vector based vaccinations and therapeutics.

The invention relates to a processing method of a lutetium yttrium silicate crystal. The processing method comprises the following steps: selecting a polishing solution; adding a dispersing agent and a pH regulator into the polishing solution; fixing the cut yttrium lutetium silicate crystal on processing equipment; through the steps of the rough polishing stage, the fine polishing stage and the post-treatment stage, the nanoscale surface shape precision RMS can be better than 0.5 nm, the sub-nanoscale surface roughness Ra is smaller than or equal to 0.55 nm, the surface quality of the LYSO crystal is remarkably improved, and the problem that at present, the surface roughness Ra is smaller than or equal to 0.55 nm is solved. The polishing technology for the LYSO crystal mainly comprises traditional mechanical polishing, chemical mechanical polishing is difficult to meet the requirements of the LYSO crystal for high-precision machining and difficult control of technological parameters, the use of chemical reagents may cause pollution to the environment, and the adaptability is poor when the LYSO crystal is in a complex shape. And high-precision deterministic processing cannot be realized.

The invention relates to the non-therapeutical use of phosphatidylcholine and / or lyso- phosphatidylcholine for increasing the nutrient retention of at least one Long-Chain Fatty Acid (LCFA) in an animal or a human, or to a use of phosphatidylcholine and / or lyso- phosphatidylcholine for increasing the thermal stability of at least one Long-Chain 5 Unsaturated Fatty Acid (LC-UFA). The invention further relates to an ingestible composition, and a method of preparation of such a composition.

This invention discloses a preservation solution for preserving sample DNA at room temperature and its preparation method, belonging to the field of biological sample preservation technology. The preservation solution comprises: a lysis system, a nucleic acid protection system, and a buffering and stabilizing system; the lysis system comprises a complex surfactant and an enzymatic hydrolysis aid; the complex surfactant comprises: polyether polyol fatty acid ester and cocamidopropyl hydroxysulfonate betaine; the enzymatic hydrolysis aid comprises lysozyme Lyso-V and proteinase K; the nucleic acid protection system comprises: nitrogen-heterocyclic polyamine-carboxylic acid derivatives and dextran sulfate; the nitrogen-heterocyclic polyamine-carboxylic acid derivatives comprise: 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''tetraacetic acid, 1,4,7-triazacyclononane-N,N',N''-triacetic acid, disodium ethylenediaminetetraacetate-nitrocyclic derivative, and diethylenetriaminepentaacetic acid-piperazine derivative; the buffering and stabilizing system comprises: an amphoteric buffer and a polymeric stabilizer; the amphoteric buffer comprises: 2-(N-morpholino)ethanesulfonic acid and N-tris(hydroxymethyl)methylglycine.

The invention belongs to the field of inspection, and particularly relates to a composite marker for detecting biomarkers related to lysosomal storage diseases based on UPLC-MS / MS. The composite marker comprises a marker and an isotope internal standard substance, wherein the marker comprises glucosyl sphingosine, spherical glycosyl sphingosine, hemolytic sphingomyelin, N-palmitoyl-O-phosphorylcholine-serine, 7-ketocholesterol, cholestane-3, 5, 6-triol, galactosyl sphingosine, C26-ceramide, hemolytic GM1 ganglioside and hemolytic GM2 ganglioside, and the molecular weight of the marker is 8000-9000. The isotope internal standard substance is prepared from < 13 > C < 6 >-glucosyl sphingosine, D7-spherical glycosyl sphingosine, D9-lysosphingomyelin, D9-N-palmitoyl-O-phosphorylcholine-serine, D7-7-ketocholesterol, D7-cholestane-3, 5, 6-triol and D5-galactosyl sphingosine. The invention also comprises a UPLC-MS / MS method for detecting the composite marker. According to the invention, simultaneous detection of multiple biomarkers related to lysosomal storage diseases is realized, and the method is simple and convenient to operate, high in flux and low in cost.

A method for quantifying Hex4, lyso-GM1, Fuc-GlcNAc-Asn, or lyso-sulfatide included in cerebrospinal fluid, the method including adding an internal standard substance to a solution including the cerebrospinal fluid, submitting the solution including the cerebrospinal fluid, to which the internal standard substance has been added, to liquid chromatography to obtain an eluate, and subjecting the eluate to mass analysis.

Compositions and methods for inducing immune tolerance to proteins and subsequence administration of the proteins are disclosed. Compositions comprise proteins complexed with liposomes comprising PS and PC, wherein at least part of the PS is present as lyso-PS.

The present invention relates to an anti-inflammatory extract of soil microorganisms or a fraction thereof, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria. The invention further relates to compositions comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS. The invention further relates to pharmaceutical compositions comprising the anti-inflammatory extract of soil microorganisms or a fraction thereof or a composition comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS as an active ingredient. The invention further relates to an anti-inflammatory extract of soil microorganisms or a fraction thereof or a pharmaceutical composition comprising an anti-inflammatory extract of soil microorganisms or a fraction thereof or a composition comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS as an active ingredient for use as a medicament, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria. The invention further relates to compositions comprising an anti-inflammatory extract of soil microorganisms or a fraction thereof, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria.

The invention discloses a liposome nanoparticle in-vitro release medium and an in-vitro release test method. The liposome nanoparticle in-vitro release medium comprises the following components: a buffer solution and lipid, wherein the lipid is one or more of DOPE and at least one of DOPS, POPS, DOPC, DSPC, Egg PC, Lyso PC, DPPC, DPPE, DMPE, POPE, DOPG and DGPG. The liposome nanoparticle in-vitro release medium simulates an in-vivo environment in vitro, and an in-vitro release curve is drawn by detecting the contents of nucleic acid, polypeptide and antibody, so that the liposome nanoparticle in-vitro release medium has important significance on prescription development and quality research of liposome nanoparticles.

This invention discloses a method for determining related substances in liposome adjuvants and its applications, relating to the field of pharmaceutical testing. The method is a high-performance liquid chromatography (HPLC) method. Mobile phase A comprises an aqueous solution containing trifluoroacetic acid; mobile phase B comprises a mixed solution of methanol and acetonitrile containing trifluoroacetic acid. Through a specific elution procedure, the simultaneous detection of multiple target substances in liposome adjuvants is achieved, including oleic acid, ketocholesterol, two isomers of Lyso-DOTAP, and two isomers of Lyso-DOPC. This method exhibits good specificity, high sensitivity, and good resolution, providing a new approach for evaluating the efficacy, safety, and stability of liposome adjuvants.

The present invention relates to: a biomarker composition for diagnosing multiple cancers, the biomarker composition comprising metabolites; and an artificial intelligence-based method for providing information for diagnosing multiple cancers and, more specifically, to a biomarker composition for diagnosing multiple cancers and an artificial intelligence-based method for providing information for diagnosing multiple cancers, the biomarker composition comprising at least five biomarkers selected from the group consisting of valine (Val), glutamate (Glu), N-methyl-2-pyridone-5-carboxamide (2PY), tryptophan (Trp), kynurenine (KN), acetylcarnitine (AC), hexanoylcarnitine (HC), octanoylcarnitine (OC), decanoylcarnitine (DC), lauroylcarnitine (LAC), myristoylcarnitine (MC), palmitoylcarnitine (PC), 16:0 Lyso PC (LPC16), 18:0 Lyso PC (LPC18), 15:0-18:1 PC (15PC), 16:0 SM (16SM), 18:1 SM (18SM), and 24:1 SM (24SM).

The invention discloses an excimer proportional fluorescent probe capable of realizing high-selectivity lysosome polarity imaging in cells as well as a preparation method and application thereof, the fluorescent probe is marked as Lyso-PRG and is prepared only through one-step reaction, and the fluorescent probe is easy to obtain synthetic raw materials, simple to operate and easy to popularize. The fluorescent probe is applied to preparation of a reagent or a detection element for detecting polarity change in proportional lysosome. According to the fluorescent probe disclosed by the invention, a monomer-excimmer imaging mechanism is creatively applied to the design of the polar imaging probe for the lysosome, so that the obtained fluorescent probe shows extremely low background noise, ultrahigh Stocks displacement (130nm) and double-color proportional fluorescent properties; on the basis of the fluorescent probe, a quaternary amine positive charge structure is introduced, so that the quaternary amine positive charge structure can be prevented from being combined with a lipid membrane and lipid droplets, excellent lysosome selectivity is shown, and further remarkable advantages in sensitivity, resolution and accuracy of lysosome polarity detection are shown; the method has a wide application prospect in the fields of lysosome polarity-related pathological research, efficacy evaluation and new drug research and development.

The present invention provides methods for detecting multiple biomarkers indicative for lysosomal storage diseases from a dried blood spot. In particular, the present invention provides a method that is suited for detecting multiple biomarkers, each indicative for the presence of a distinct lysosomal storage disease in a subject, based on a single sample preparation procedure. In particular, the method allows simultaneous extraction of different biomarkers such as Lyso-Gb1 (GlcSph). Lyso-Gb3, and others from a dried blood spot sample. The invention further provides a kit of parts comprising means for conducting the methods subject of the invention. Finally, the invention provides a set of reference ranges for Lyso-Gb1 (GlcSph) and Lyso-Gb3 in healthy subjects starting from dried blot spot samples.

Provided are a composition containing a lipid nanoparticle (LNP) and an mRNA, and use thereof in the treatment of Gaucher disease. The composition contains an LNP and an mRNA, and the mRNA is encapsulated in the LNP or associated with the LNP, wherein the mRNA contains a nucleotide sequence encoding GBA1. The composition can effectively increase the expression and activity of β-glucocerebrosidase (β-GCase) in the serum and multiple target organs of a subject, and reduce the level of glucosylsphingosine (Lyso-GL1) therein. In addition, the composition possesses a relatively long half-life and has application prospects in the treatment of Gaucher disease.

The invention discloses an application of lysolecithin in preparation of a product for protecting immune stress of pigs, the body health of the pigs in an immune stress scene can be protected by feeding the pigs with the product containing the lysolecithin, and the damage of intestinal tracts and livers of the pigs caused by lipopolysaccharide stimulation can be relieved, so that the immune stress of the pigs can be protected. The purpose of preventing the pig intestinal injury and / or liver injury can be achieved, the purpose of treating the pig intestinal injury and / or liver injury can also be achieved, and the pig intestinal injury and / or liver can be protected.

The present invention discloses a kind of excimer ratio fluorescent probe, preparation method and application that can be used for high-selectivity lysosomal polarity imaging in cells, and the fluorescent probe is recorded as Lyso-PRG, and only one-step reaction is required to prepare it, and its synthetic raw materials are easy to obtain, simple to operate, and easy to promote. The fluorescent probe is applied to prepare ratio-type lysosomal intracellular polarity change detection reagent or detection element. The fluorescent probe of the present invention pioneered the use of monomer-excimer imaging mechanism for the design of lysosomal polarity imaging probe, and then the obtained fluorescent probe shows extremely low background noise, ultra-high Stocks shift (130nm) and dual-color ratio fluorescence properties; and based on the basis of the fluorescent probe, by introducing quaternary amine positive charge structure, it can be avoided to be combined with lipid membrane and fat droplet, showing excellent lysosomal selectivity, and then in the sensitivity, resolution and accuracy of lysosomal polarity detection, it shows significant advantages, and it has broad application prospects in lysosomal polarity-related pathological research, drug efficacy evaluation and new drug research and development fields.

The present relates to lipases having galactolipase activity and no / low lyso-phospholipase activity. More particularly, the present invention relates to the use of such lipases for generation of emulsifying components from endogenous flour lipids.

Disclosed are methods of preparing liposomes without size exclusion steps resulting in small sized liposomes exhibiting narrow size distribution and compositions of same. The methods utilize liposome formation from lyso-PS, PS, and PC as the only phospholipids.

The application provides a lysophosphatidylcholine (LPC) kit with anti-coccidiosis effect, and discloses a diagnosis marker for Eimeria tenella infected chicken, which is characteristic phospholipid and sphingolipid molecules, and specifically phosphatidylcholine (PC), lysophosphatidylcholine (LPC) and sphingomyelin (SM). Meanwhile, the application provides a medicine composition for improving the survival rate of chicks infected with Eimeria tenella, which is characterized by containing effective amounts of 18:2 LPA, 18:1 LPC, 22:0 LPC, 16:0 18:1 PC, 16:0 18:2 PC and d18:1 16:0 SM. The application screens characteristic phospholipid and sphingolipid molecules in the cecum tissue of chicken infected with Eimeria tenella for the first time, and verifies the curative effect of phosphatidylcholine (PC), lysophosphatidylcholine (LPC) and sphingomyelin (SM) on acute infection of chicks with Eimeria tenella, which has great market development prospect.

The process of reuse lysogoma, applied in the pre-treatment of already degummed oil for subsequent PLA treatment and transesterification of biodiesel object of the present invention comprises a pre-treatment step through the reuse of lysogoma originating from the PLA enzymatic treatment in the oil already degummed. The use of lysogoma containing active enzymes resulting from the enzymatic treatment (11) on the already degummed oil (40) aims to reduce the levels of phosphorus, calcium and magnesium. In short, the use of lysogoma (10) is presented as a pre-treatment as it aims to reduce the contents of phosphorus, calcium and magnesium present in the degummed oil. Furthermore, this new process impacts the reuse of a byproduct with no commercial value that helps to significantly reduce the contents of phosphorus, calcium and magnesium, which negatively interfere in the processes, especially in the PLA enzymatic treatment and transesterification steps for biodiesel production.

Irinotecan phospholipid liposomes with improved storage stability are provided. with related methods of treatment and manufacture. The irinotecan liposomes can have reduced formation of lyso-phosphatidylcholine (lyso-PC) during storage. and prior to administration to a patient.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a double-color fluorescent probe for monitoring polarity and viscosity fluctuation of lysosome in a cell apoptosis process as well as a preparation method and application of the double-color fluorescent probe. The novel two-color fluorescent probe Lyso-DCPV is successfully synthesized, and the probe can respond to dynamic changes of polarity and viscosity at the same time. In the probe, N, N-diethyl coumarin serves as a polarity sensitive module (emitting green fluorescence), and N, N-diethyl coumarin and N-propyl quinoline are connected through a twisted double bond to serve as a viscosity sensitive module (emitting red fluorescence). The green fluorescence of the Lyso-DCPV is gradually weakened along with the increase of the polarity, and the red fluorescence of the Lyso-DCPV is remarkably enhanced along with the increase of the viscosity, so that the Lyso-DCPV can simultaneously track the change of the polarity and the viscosity. In addition, the Lyso-DCPV has remarkable lysosome targeting capacity in living cells, and the capacity of tracking polarity and viscosity changes in the living cell lysosome in real time in the drug-induced apoptosis process is achieved for the first time.