40 results about "Lyso-" patented technology

A method for enhancing the light yield of a single crystal of cerium doped lutetium yttrium orthosilicate (LYSO) in response to irradiation with high energy radiation includes diffusing oxygen into the crystal by heating the crystal for a period of time in an ambient containing oxygen. This process of thermal oxygenation of the crystal effectively supplies oxygen to fill at least some of the oxygen vacancies in the body of monocrystalline LYSO. A scintillation detector comprises a monocrystalline body of LYSO enhanced by oxygen diffusion into the crystal.

A new enzymatic process for preparing 1,2-diacylated phospholipids using an enzyme preparation possessing phospholipase activity towards acylation at the sn-1 and sn-2 sites in a microaqueous reaction system. More particularly, the 1,2-diacyl-phospholipids produced according to the esterification / transesterification process are obtainable in high yield and purity and carry identical desired carboxylic acid, preferably fatty acid, acyl groups at the sn-1 and sn-2 positions. The process involves esterification / transesterification (acylation) of a glycerophospholipid, preferably glycerophosphoryl choline (GPC) with a desired carboxylic acid, preferably fatty acid, or their derivatives in the presence of the above mentioned appropriate enzyme preparation. The process of the invention further relates to a process for the production of 1-acyl-2-lyso-glycerophospholipid, preferably 2-lyso-PC by reacting glycerophospholipid, preferably glycerophosphoryl choline (GPC) with a desired carboxylic acid, preferably fatty acid, or their derivatives in the presence of a sn-1 specific phospholipase (PLA1 or PLA1,2) and a solvent, in a microaqueous medium.

The invention discloses a process for growing cerium-doped LYSO scintillating crystals by using a molybdenum crucible, which belongs to the technical field of scintillating crystal preparation. The process for growing scintillating crystals comprises the following steps: (a) putting a solid-phase reaction material into the molybdenum crucible; (b) putting the crucible into a vacuum furnace, and vacuumizing the vacuum furnace until the pressure of the inside of the furnace is less than or equal to 10 Pa; (c) filling flowing weak reductive protective gas in the furnace; (d) heating the inside of the furnace, and carrying out at least primary air exchange in the process of heating, wherein the air exchange includes vacuumizing the inside of the furnace until the pressure is less than or equal to 10 Pa, and then filling flowing weak reductive protective gas in the furnace again; and (e) further heating the inside of the furnace until the solid-phase reaction material is melted, and carrying out crystal growth. The process for growing LYSO scintillating crystals overcomes technical problems; and by using the process, the molybdenum crucible both can resist oxidation and erosion of molten LYSO raw materials, then high-quality LYSO scintillating crystals grow, and therefore, the production cost is greatly reduced, and the process is relatively simple and easy to control.

A method is disclosed for the generation of triacylglycerols from gums that have been separated from an oil product. The gums are treated with an enzyme having PLC activity, which results in the formation of diacylglycerols and phosphates, and treated with an enzyme having PLA activity, which results in the formation of lyso-phospholipids and free fatty acids. The diacylglycerols and the free fatty acids from these two separate reactions then combine in the presence of the enzymes to generate new triacylglycerol molecules.

The invention discloses a two-photon fluorescence dye, a preparation method thereof and an application. The two-photon fluorescence dye takes carbazole as a parent body, and the structure of the two-photon fluorescence dye is as shown in the description. According to the two-photon fluorescence dye, in the wide concentration range, one-photon and two-photon fluorescence signals and the concentration of the fluorescence dye have good linear relationships. Two-photon confocal fluorescence micro-imaging experiments show that the fluorescence dyes Lyso-MCO and Lyso-NCO have good permeability for MCF (macrophage chemotactic factor) cells, lysosomes in the cells can be effectively positioned (positioning coefficients are 0.9475 and 0.9398), and the two-photon fluorescence dye is applicable to two-photon fluorescence imaging of the lysosomes in the cells. Compared with a commercial lysosome dye (Lysosome Tracker Red), the two-photon fluorescence dyes Lyso-MCO and Lyso-NCO are better in photo-stability.

The invention relates to the technical field of crystal growth, in particular to a calcium fluoride crystal containing europium, a preparation method and application. The calcium fluoride crystal containing europium, provided by the invention, can be irradiate with Co60; the light yield of the crystal is measured with an RCA8850 photomultiplier; the result shows that the calcium fluoride crystal containing europium, provided by the invention, has the excellent scintillating crystal property of emitting twinkling fluorescent light of a certain wave length after absorbing the energy of high-energy particles on the condition that the content of the crystal is low. Especially, the relative light output of the crystal is equal to that of an LYSO scintillating crystal. Besides, as the content is low, the calcium fluoride crystal containing europium is beneficial to growth of high optical mass crystals. The invention further provides the preparation method and the application of the calcium fluoride crystal containing europium.

Compositions and methods for inducing immune tolerance to proteins and subsequence administration of the proteins are disclosed. Compositions comprise proteins complexed with liposomes comprising PS and PC, wherein at least part of the PS is present as lyso-PS.

The invention discloses a multifunctional scintillation crystal testing platform comprising a pedestal, a replaceable testing stand, and a hidden box. The pedestal is mainly used for providing power for the replaceable testing stand, obtaining data collected by the replaceable testing stand, processing the data and then sending the data to a computer through a USB data interface. The replaceable testing stand is formed by cooperation of a silicon photomultiplier array with different light guides by an optical coupling agent. Tested crystals are arranged above the light guides by on air coupling. A large LYSO support is embedded into the hidden box and is used for placing an LYSO crystal block needed by testing. On the basis of a new-generation solid photoelectric sensor SiPM technology, no radioactive source is needed and performances of crystals with different sizes and forms can be measured rapidly and precisely by replacing different light guides, wherein the crystals include a single crystal bar, single crystal arrays with different sizes, a plurality of crystal arrays with standard dimensions, and a plurality of crystal line arrays and the like. With the platform, the crystal product yield of crystal manufacturers can be improved substantially and the production cost and crystal products can be lowered.

The invention discloses an organelle targeting photosensitizer capable of activating pyroptosis of tumor cells and a preparation method and application thereof. The Mito-ZS photosensitizer is a mitochondrial targeting photosensitizer; the Lyso-ZS photosensitizer is a lysosome targeting photosensitizer; the ER-ZS photosensitizer is an endoplasmic reticulum targeting photosensitizer. The photosensitizer activates a pyroptosis pathway by performing precise targeting therapy on organelles in tumor cells so that photodynamic therapy and immunotherapy strategies are perfectly combined, and the photosensitizer has a wide application prospect in the field of cancer therapy.

The invention belongs to the field of fluorescence detection, and relates to a double-targeting fluorescent probe capable of simultaneously visualizing plasma membranes and lysosomes and application of the double-targeting fluorescent probe, the probe is superior to the application of the conventional probe which can only display one observation substance, and the probe can simultaneously mark the plasma membranes and the lysosomes and enable the plasma membranes and the lysosomes to display different colors. Mem-Lyso can be used for marking plasma membranes and lysosomes with red and yellow respectively, so that the relative positions of two organelles of the zebra fish are displayed through real color imaging. More importantly, the Mem-Lyso has the capability of spatially distinguishing the content of cytomembrane cholesterol (CL) due to the double-targeting characteristic, and a potential detection tool is provided for biological research and diagnosis of related diseases, so that research on the relationship between the plasma membrane and the lysosome has important significance for related biological research.

The invention provides a flickering layered photography and ray measuring system and a union image and ray measuring system. A flickering layered photographic camera comprises a series of detectors and multiple radiometers for detecting target bodies, wherein BGO crystals or LYSO crystals, gamma-ray measuring software or SPECT, and X-ray determinators or CT are adopted in the series of detectors, and the series of detectors are used for detecting positive electrons, negative electrons and X-rays in the target bodies. The multiple radiometers can achieve the PET positive electron detection function and can detect and count residual gamma-rays and X-rays in a multi-probe and multilayer mode, wherein the residual X-rays are in the environment, and therefore the static and / or dynamic positive electron developing and SPECT function or the gamma-ray detection function or the X-ray quantitative analysis function or the CT function is achieved.

The invention discloses a preparation method of human hair melanosomes derivatives (HHMs). The preparation method comprises the following steps of step 1, pretreatment: crushing, cleaning and drying human hair; step 2, hydrothermal reaction: carrying out hydrothermal reaction on the pretreated human hair in a NaOH solution; and 3, separation: carrying out centrifugal separation and vacuum drying on a solid sample material obtained after the hydrothermal reaction to obtain HHMs. The invention relates to a preparation method of a human hair melanosomes derivative-lysozyme (HHMs-Lyso) composite material. The preparation method comprises the following steps of uniformly dispersing prepared HHMs into deionized water to obtain a first feed liquid; dispersing lysozyme into deionized water to obtain second feed liquid; and mixing the first feed liquid and the second feed liquid in an isopyknic manner to obtain the HHMs-Lyso. According to the HHMs prepared through the method, MRSA can be efficiently killed by loading Lyso, and meanwhile tissue repair can be promoted through the HHMs.

The invention discloses an experimental device for shortening flicker decay time of a LYSO crystal, and the experimental device comprises a vertically arranged thermal insulation sleeve, wherein the thermal insulation sleeve comprises a vertically arranged sample placing cylinder, the inner area of the sample placing cylinder is a heating cavity, the sample placing cylinder is made of a thermallyconductive insulating material, a crystal fixing mechanism is provided in the middle of the inner surface of the sample placing cylinder, and heating wires are evenly arranged on the outer surface ofthe sample placing cylinder to form a heating layer. The invention also discloses an experimental method of the above experimental device. The invention detects the flicker decay time of the heated sample crystal by setting a photodetector directly above the sample placing cylinder, and finds that the flicker decay time has changed from the original 36-42ns to 8-12ns, thereby overcoming the technical bias that the persons skilled in the art believe that LYSO crystal flicker cannot be used for high-repetition experiments.

Compositions and methods for inducing immune tolerance to proteins and subsequence administration of the proteins are disclosed. Compositions comprise proteins complexed with liposomes comprising PS and PC, wherein at least part of the PS is present as lyso-PS.

The present invention provides a medicine and an apparatus which can detect the sites and spreading of in vivo lipids by a two-dimensional image, thereby allowing diagnosis of diseases due to accumulation of lipids. According to the present invention, provided are a medicine for detecting in vivo lipids, comprising one or more components such as oxLDL and lyso PC, as an effective component, selected from a sulfonic acid blue dye having a radical —SO3−, a homidium, Metanyl yellow and Lissamin fast yellow, and an angioscopic system for obtaining a fluorescent image, which comprises a mercury-xenon lamp light source, a quartz fiberscope wherein the light guide part has 100 to 200 fibers and the image guide part has 7000 to 9000 fibers, a rotational exciting filter, an emitting filter, and a high-sensitive digital camera for picking up the fluorescence as a two-dimensional color image.

The invention relates to a method for measuring the temperature change of an organelle. The method comprises the steps of preparing a temperature-sensitive water-soluble CdTe / CdS / ZnS quantum dot solution, and coating silicon dioxide on the temperature-sensitive water-soluble CdTe / CdS / ZnS quantum dot solution by using a reverse microemulsion method to obtain a CdTe / CdS / ZnS@SiO2 particle solution; then preparing a positioning probe CdTe / CdS / ZnS@SiO2-Cyto, a lysosome targeting positioning probe CdTe / CdS / ZnS@ SiO2-Lyso or a mitochondria targeting positioning probe CdTe / CdS / ZnS@SiO2-Mito for ingesting cytoplasm in cells; respectively taking positioning probes into cells through a liposome transfection method, carrying out targeted positioning in intracellular cytoplasm or on different organelles through different targeted modifications, combining with the temperature-sensitive characteristics of CdTe / CdS / ZnS quantum dots, and realizing acquisition of temperature-sensitive fluorescence information of quantum dots of a targeted organelle through the action of combining an inverted fluorescence microscope with slit locking of a spectrograph, so that temperature change information in cytoplasm or temperature change information of the targeted localized organelle is obtained. According to the invention, targeting cytoplasm and specific organelles (such as lysosome and mitochondria) and monitoring the temperature change of the cytoplasm and specific organelles are realized.

The invention relates to mRNA therapy for the treatment of Fabry disease. mRNAs for use in the invention, when administered in vivo, encode human the α-galactosidase A (GLA), isoforms thereof, functional fragments thereof, and fusion proteins comprising GLA. mRNAs of the invention are preferably encapsulated in lipid nanoparticles (LNPs) to effect efficient delivery to cells and / or tissues in subjects, when administered thereto. mRNA therapies of the invention increase and / or restore deficient levels of GLA expression and / or activity in subjects. mRNA therapies of the invention further decrease levels of toxic metabolites associated with deficient GLA activity in subjects, namely Gb3 and lyso-Gb3.

The invention discloses a device capable of converting a high-energy X ray image into a visible light image, which relates to a high-energy physical technique. The device comprises a plurality of parallel LYSO crystal columns to form an array, the incidence plane is parallel with the outgoing plane, and the incidence plane and the outgoing plane are vertical to the vertical direction of crystal columns. The device has the advantages of converting invisible high-energy X ray into visible light for the convenience of testing and achieving the spatial resolving power of sub-millimeter and the time resolving power of tens of nanoseconds.

Compositions and methods for inducing immune tolerance to proteins and subsequence administration of the proteins are disclosed. Compositions comprise proteins complexed with liposomes comprising PS and PC, wherein at least part of the PS is present as lyso-PS.

The invention discloses a lysosome fluorescent probe of chalcone as well as a preparation method and application of the lysosome fluorescent probe, and belongs to the technical field of organic molecular fluorescent probes. The preparation method comprises the following steps: adding 2 '-hydroxychalcone HCA and 1, 3-dibromopropane into an organic solvent for reaction, carrying out silica gel column chromatography separation to obtain an intermediate Br-HCA, adding the intermediate Br-HCA and morpholine into the organic solvent for reaction, and carrying out silica gel column chromatography separation to obtain the lysosome fluorescent probe Lyso-HCA. The preparation method of the fluorescent probe is simple, rapid and low in cost, the prepared fluorescent probe is low in background interference, high in detection sensitivity and high in excitation capacity when being applied to cell fluorescence imaging, and the fluorescent probe is good in stability to an acid-base environment and good in specificity to lysosome fluorescence imaging. The probe provides a tool with a wide application prospect for cell lysosome fluorescence imaging research.

The invention provides a fluorescent dye for lysosome labeling and its synthesis method and application. The fluorescent dye uses naphthalimide as a fluorophore and introduces an N-(2-aminoethyl) at its N position. Phyloline substituents, two azetidine substituents were introduced at the 4,5-position, and a new type of lysosome fluorescent dye—Lyso-DAze was designed and synthesized. Its structural formula is shown in (1). The fluorescence of the dye The emission wavelength is 498nm, and it has very excellent fluorescence stability and high fluorescence intensity, and can realize the specific labeling of lysosomes in living cells under the condition of relatively low concentration (500nM). The Lyso-DAze dye satisfies the high stability and high brightness requirements of the current super-resolution technology for fluorescent dyes, making lysosome visualization have a very broad application prospect in basic research on the occurrence and development of diseases.

The invention relates to a preparation method of yttrium lutetium cerium oxide for scintillation crystals, and belongs to the technical field of rare earth extraction and separation. The preparation method of the present invention comprises the following steps: Step 1: According to the stoichiometric ratio of the three rare earth elements of yttrium, lutetium and cerium, the stripping solutions of the three rare earth ions are evenly mixed, and the pH value of the solution is adjusted to 2 to 3 with ammonia water, and heated. Obtain a solution; Step 2: Prepare the oxalic acid solution according to the molar ratio of the total rare earth and the precipitant oxalic acid as 1:1.8-2.2, and heat the oxalic acid solution to obtain the b solution; Step 3: Slowly add the a solution under the condition of stirring into the solution b, stirred, left to age for 3-6 hours, and filtered to obtain the precipitate of yttrium-lutetium oxalate; step 4: the precipitate of yttrium-lutetium oxalate was burned in a muffle furnace at 800-1200°C for 2-4 hours to obtain the oxidized Yttrium lutetium cerium powder. The yttrium lutetium cerium oxide powder prepared by the invention has the advantages of uniform distribution of rare earth elements, precise and adjustable element content, and can provide raw materials for the preparation of scintillation crystals such as LYSO and LuYAP.

The invention provides an intelligent daily quality control method for LYSO crystal PET, and relates to the technical field of PET quality control. The method comprises the steps: intelligent data collection: collecting LYSO background data according to preset data collection parameter information, and storing the LYSO background data; data intelligent analysis and processing: performing statistics on information in the data to obtain a three-dimensional heat statistical graph, sending the three-dimensional heat statistical graph to a pre-trained deep learning neural network for diagnosis, obtaining an abnormal diagnosis result, and generating a DailliyQC report; and data decision and early warning: classifying the diagnosed abnormal conditions, judging whether the abnormal conditions influence the overall performance and reach the danger level or not, giving a danger alarm for the abnormal conditions reaching the danger level, and recording the abnormal conditions which do not reach the danger level and do not influence the overall performance. According to the method, an extra radioactive source is not needed, on-site operation of an operator is not needed, the data acquisition,processing, analysis and automatic identification of the DailliyQC are automatically realized, and the decisions and early warning are made.

The invention relates to a silicon-based rhodamine nitric oxide fluorescent probe, the structure of which is as follows: the Lyso-SiRB-NO (formula II) has the function of lysosome localization. The advantages of the present invention: the excitation and emission wavelengths of the silicon-based rhodamine nitric oxide probe of the present invention are located in the near-infrared region, which can reduce the interference of the autofluorescent background of the organism when applied to biological imaging, and have strong penetrating ability. There is less damage to living tissues or cells; another advantage of the probe is that it can specifically detect nitric oxide molecules in lysosomes, providing a reliable research tool for studying the physiological effects of nitric oxide molecules.

The present invention relates to lyso-diacylglyceryltrimethylhomoserine (lyso- DGTS) or derivatives thereof for use in the treatment of atherosclerotic cardiovascular disease, and further provides particular such lyso-DGTS derivatives.

The present invention relates to lyso-diacylglyceryltrimethylhomoserine (lyso-DGTS) or derivatives thereof for use in the treatment of atherosclerotic cardiovascular disease, and further provides particular such lyso-DGTS derivatives.

The invention discloses a two-photon fluorescence dye, a preparation method thereof and an application. The two-photon fluorescence dye takes carbazole as a parent body, and the structure of the two-photon fluorescence dye is as shown in the description. According to the two-photon fluorescence dye, in the wide concentration range, one-photon and two-photon fluorescence signals and the concentration of the fluorescence dye have good linear relationships. Two-photon confocal fluorescence micro-imaging experiments show that the fluorescence dyes Lyso-MCO and Lyso-NCO have good permeability for MCF (macrophage chemotactic factor) cells, lysosomes in the cells can be effectively positioned (positioning coefficients are 0.9475 and 0.9398), and the two-photon fluorescence dye is applicable to two-photon fluorescence imaging of the lysosomes in the cells. Compared with a commercial lysosome dye (Lysosome Tracker Red), the two-photon fluorescence dyes Lyso-MCO and Lyso-NCO are better in photo-stability.

Compositions and methods for inducing immune tolerance to proteins and subsequence administration of the proteins are disclosed. Compositions comprise proteins complexed with liposomes comprising PS and PC, wherein at least part of the PS is present as lyso-PS.