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Method for measuring temperature change of organelle

A technology of temperature change and measurement method, which is applied in the field of measurement of cell organelle temperature change, can solve problems such as the failure to realize the characterization of cell organelle temperature, and achieve the effects of easy mastery of the process, simple experimental equipment, and simple preparation of raw materials

Active Publication Date: 2021-04-20
XI AN JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The Chinese patent with the publication number CN201810015252.X "Preparation method and application method of a core-shell nanocrystalline cell temperature sensor" uses Nd in rare earth luminescence 3+ The temperature-sensitive characteristic relationship of ion spectrum realizes the measurement of temperature. Firstly, rare earth luminescent particles doped with ions with temperature-sensitive characteristics are prepared, and then the temperature-sensitive luminescent particles are taken into cells and the corresponding relationship between luminous intensity and temperature is established. , monitor the temperature change in the cell by testing the luminous intensity change of the ingested particles; this method monitors the temperature of the cell after the temperature-sensitive rare earth fluorescent particles are ingested into the cell, and does not realize the characterization of the organelle temperature on the subcellular scale

Method used

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  • Method for measuring temperature change of organelle
  • Method for measuring temperature change of organelle
  • Method for measuring temperature change of organelle

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Effect test

Embodiment 1

[0021] Embodiment 1, a method for measuring the temperature change of organelles, comprising the following steps:

[0022] The first step is to prepare a temperature-sensitive water-soluble CdTe / CdS / ZnS quantum dot solution, and prepare a CdTe / CdS / ZnS quantum dot solution located in the visible light emission by layer-by-layer coating by the aqueous phase method;

[0023] In the second step, use the reverse microemulsion method to coat the thermosensitive water-soluble CdTe / CdS / ZnS quantum dot solution prepared in the first step with silica: 7.5mL of cyclohexane, 1.77mL of triton 100 and 1.8 mL of n-hexanol were mixed, and 200 μL of the temperature-sensitive water-soluble CdTe / CdS / ZnS quantum dot solution prepared in the first step was added to the mixed solution, followed by 240 μL of 25 wt% ammonia water and stirred at a medium speed After a period of time, 100 μL of tetraethyl orthosilicate was finally added to the above solution. At this point, the reaction flask was place...

Embodiment 2

[0028] Embodiment 2, a method for measuring the temperature change of organelles, comprising the following steps:

[0029] The first step is to prepare a temperature-sensitive water-soluble CdTe / CdS / ZnS quantum dot solution, and prepare a CdTe / CdS / ZnS quantum dot solution located in the visible light emission by layer-by-layer coating by the aqueous phase method;

[0030] In the second step, use the reverse microemulsion method to coat the thermosensitive water-soluble CdTe / CdS / ZnS quantum dot solution prepared in the first step with silica: 7.5mL of cyclohexane, 1.77mL of triton 100 and 1.8 mL of n-hexanol were mixed, and 200 μL of the temperature-sensitive water-soluble CdTe / CdS / ZnS quantum dot solution prepared in the first step was added to the mixed solution, followed by 240 μL of 25 wt% ammonia water and stirred at a medium speed After a period of time, 100 μL of tetraethyl orthosilicate was finally added to the above solution. At this point, the reaction flask was place...

Embodiment 3

[0036] Embodiment 3, a method for measuring the temperature change of organelles, comprising the following steps:

[0037] The first step is to prepare a temperature-sensitive water-soluble CdTe / CdS / ZnS quantum dot solution, and prepare a CdTe / CdS / ZnS quantum dot solution located in the visible light emission by layer-by-layer coating by the aqueous phase method;

[0038] In the second step, use the reverse microemulsion method to coat the thermosensitive water-soluble CdTe / CdS / ZnS quantum dot solution prepared in the first step with silica: 7.5mL of cyclohexane, 1.77mL of triton 100 and 1.8 mL of n-hexanol were mixed, and 200 μL of the temperature-sensitive water-soluble CdTe / CdS / ZnS quantum dot solution prepared in the first step was added to the mixed solution, followed by 240 μL of 25 wt% ammonia water and stirred at a medium speed After a period of time, 100 μL of tetraethyl orthosilicate was finally added to the above solution. At this point, the reaction flask was place...

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Abstract

The invention relates to a method for measuring the temperature change of an organelle. The method comprises the steps of preparing a temperature-sensitive water-soluble CdTe / CdS / ZnS quantum dot solution, and coating silicon dioxide on the temperature-sensitive water-soluble CdTe / CdS / ZnS quantum dot solution by using a reverse microemulsion method to obtain a CdTe / CdS / ZnS@SiO2 particle solution; then preparing a positioning probe CdTe / CdS / ZnS@SiO2-Cyto, a lysosome targeting positioning probe CdTe / CdS / ZnS@ SiO2-Lyso or a mitochondria targeting positioning probe CdTe / CdS / ZnS@SiO2-Mito for ingesting cytoplasm in cells; respectively taking positioning probes into cells through a liposome transfection method, carrying out targeted positioning in intracellular cytoplasm or on different organelles through different targeted modifications, combining with the temperature-sensitive characteristics of CdTe / CdS / ZnS quantum dots, and realizing acquisition of temperature-sensitive fluorescence information of quantum dots of a targeted organelle through the action of combining an inverted fluorescence microscope with slit locking of a spectrograph, so that temperature change information in cytoplasm or temperature change information of the targeted localized organelle is obtained. According to the invention, targeting cytoplasm and specific organelles (such as lysosome and mitochondria) and monitoring the temperature change of the cytoplasm and specific organelles are realized.

Description

technical field [0001] The invention belongs to the technical field of intracellular temperature measurement, in particular to a method for measuring the temperature change of organelles. Background technique [0002] The cognition of cell temperature is the basis for people to understand the influence of normal life thermal activities of organisms, and it is also the basis for people to understand life and diseases from the perspective of cells. Many studies have also begun to detect cell temperature, such as the Chinese patent "An Improved Cell Temperature Sensor and Its Manufacturing Method" with the publication number CN201610241330.9, which is formed by a heterogeneous structure made of metal materials and chemical polymers Thermocouple, the prepared 0.5-50μm needle tip probe is penetrated into the surface of living cells in the cell, and used to measure the temperature inside the cell or the temperature distribution on the cell surface; the method is to absorb the prep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01K11/3213C09K11/02C09K11/88B82Y20/00B82Y30/00B82Y40/00
Inventor 江新兵张占琴丁书江李本强
Owner XI AN JIAOTONG UNIV
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