212 results about "Organelle" patented technology

The current invention provides a biocompatible energy recharge system to normalize body functions for a mammal. Both stationary and circulatory scalar wave energy was produced and utilized to enable the entire body of a mammal to increase the energy potential of any cell in the body, to promote cell growth, to facilitate cell repair, and to reduce cell destruction, hence the cells, organs, systems and the entire body of a mammal become healthy, and to treat a disease or disorder. The invention is a combination of the generators of the three-dimensional bio-waves in the stationary and in the circulationary formats for empowering the cells in all parts of a mammal, and a method of using the 3D bio-waves uniformly to empower each organ of a mammal to restore and maintain the normal body health and function.

Methods of labelling one or more subcellular components (e.g., an organelle and / or subcellular region) in vivo are provided. Methods of labelling a protein in vivo are provided. Methods of determining a nucleic acid sequence in situ are also provided.

The invention discloses an organelle-targeted fluorescent probe for specific detection of tumor cells as well as preparation and application of the organelle-targeted fluorescent probe, and the specific structural formula of the fluorescent probe is as follows: BCY-DP shows obvious specific targeting ability of tumor cell mitochondria and has been applied to ONOO-selective imaging in living cell mitochondria; results show that the BCY-DP has high selectivity on ONOO <-> in tumor cells, can successfully distinguish the ovarian cancer cells from normal ovarian cells, and can be used for diagnosis of cancer cells.

The invention relates to the technical field of antitumor drugs, in particular to a Ru (II) complex and a preparation method and application thereof, and the Ru (II) complex has a structure as shown in a formula I. The Ru (II) complex synthesized by the method disclosed by the invention not only has relatively high cytotoxicity, but also has good photodynamic antitumor activity. After the complex is illuminated, the efficiency of the complex entering cells in an active transportation mode can be accelerated, mitochondria and endoplasmic reticulum are targeted at the same time, mitochondrial membrane potential decline and endoplasmic reticulum stress are caused to form a dual organelle damage effect, immunogenic cell death is caused, the tumor microenvironment is adjusted, and the tumor cell immunogenicity is improved. The enrichment of dendritic cells (DCs) in tumor cells is realized, the chemotactic activity of effector T cells is improved, the proportion of CD4 < + > and Foxp3 < + > cell populations is reduced, and finally the anti-tumor immune response is activated. Meanwhile, the complex provided by the invention can also induce the cell to generate pan apoptosis, retard the cell cycle in the G2 / M period and enhance the effect of the complex in inhibiting tumor proliferation.

The invention discloses a method for evaluating the environmental risk of perfluorooctane sulfonic acid by using euglena gracilis, and belongs to the field of environmental pollutant biological monitoring and ecotoxicology evaluation. The method comprises the following steps: detecting the photosynthetic activity and the movement ability of Euglena gracilis exposed to perfluorooctane sulfonic acid with environmental concentration and the organelle distribution condition of the perfluorooctane sulfonic acid in euglena gracilis cells in situ; the photosynthetic activity comprises in-situ monitoring of chlorophyll fluorescence parameters and maximum non-photochemical quenching coefficient of euglena gracilis, and quantitative analysis of chloroplast pigment content; the movement ability comprises horizontal light-driving movement speed and vertical light-driving movement speed measurement of the euglena gracilis and flagellum shedding rate calculation. The method breaks through the limitation of a traditional single autotrophic mode, reveals a regulation and control mechanism of nutritional conditions on toxic effects, provides a scientific basis for water environment safety monitoring and optimization, and has the advantages of being convenient to operate and low in cost.

The invention discloses a tellurium-doped carbon quantum dot and a preparation method and application thereof, the tellurium-doped carbon quantum dot is obtained by using 2, 2 '-diaminodiphenyl telluride as a precursor, the average particle size is 1.9 nm, the thickness is about 2.1 nm, and the surface is rich in amino groups. And fixing (4-carboxybutyl) triphenylphosphonium bromide, 3-morpholinopropionic acid or 3-((4-methylphenyl) sulfonamide) propionic acid on the surfaces of the tellurium-doped carbon quantum dots to prepare Mito-Te-CDs, Lyso-Te-CDs and ER-Te-CDs with an organelle targeting function. The Mito-Te-CDs, the Lyso-Te-CDs and the ER-Te-CDs are good in biocompatibility, can be used as fluorescent probes for targeted imaging of organelles, can be used for recognition and removal of superoxide anions in specific organelles, have a protection effect on cells and can be used for effectively treating acute kidney injury.

The present invention concerns systems, materials, and methods of cell, cell organelle and / or particle separation and / or concentration utilizing magnetic levitation to affect separation. In some embodiments, the separation and / or concentration is performed in the wells of a multi-well plate.

The invention discloses a preparation method and application of a mitochondrial targeting polypeptide modified carbon quantum dot probe, and relates to the technical field of nano biological materials and fluorescence imaging.The preparation method comprises the steps that citric acid and urea are dissolved in a dimethyl sulfoxide solution, mixed, heated, filtered, added with ethyl alcohol, centrifuged and freeze-dried, and carbon quantum dots are obtained; adding an MES buffer solution and an activating agent, reacting the polypeptide at room temperature, heating, reacting, centrifuging, dialyzing and freeze-drying to obtain polypeptide modified carbon quantum dots; the carbon quantum dots are synthesized from bottom to top, and the preparation method is simple. After polypeptide modification, the product has an obvious mitochondrial targeting effect, and the product is good in biocompatibility, so that the product can be used for imaging and dynamic tracking of living organelles. The probe developed by the invention shows obvious application potential in key cell events such as mitochondrial dynamic monitoring and the like.

The invention discloses a terahertz near-field system sample nanometer repeated positioning method, and belongs to the technical field of terahertz near-field imaging. According to the method, repeated positioning with nanoscale precision is realized through two key technical means: displacement compensation: after first scanning of a sample area is completed and reference coordinates are recorded, second scanning is carried out, and the offset of the center coordinates of a probe relative to the reference coordinates in the X-axis and / or Y-axis direction is monitored; before follow-up scanning, the reverse value of the offset is used as the compensation amount to be input into a control system so as to correct the reference coordinate; and voltage control: monitoring and controlling the voltage of the system in real time when the probe withdrawing operation is intermittently executed in the current scanning and the next scanning, and stopping probe withdrawing within one second when the voltage value rises to 180V so as to control the withdrawing distance of the probe. According to the method, the repeated positioning accuracy can be controlled within 5 nanometers, and the method is suitable for multiple times of accurate imaging of biological organelles and nano structures in semiconductors.

The invention discloses a high-activity BODIPY type photosensitizer targeting double organelles as well as a preparation method and application of the high-activity BODIPY type photosensitizer, and relates to the technical field of medical photosensitizers. The high-activity BODIPY type photosensitizer is prepared by the following steps: reacting 2, 4-dimethyl pyrrole with 4-bromobutyryl chloride to obtain a compound Br-BY containing carboxyl, then reacting with morpholine to obtain a morpholine substituted compound Mor-BY, and finally reacting with N-iodosuccinimide (NIS) to obtain a single atom I substituted BODIPY compound Mor-IBY, namely the target photosensitizer. The photosensitizer prepared by the invention can target mitochondria and lysosome in cancer cells at the same time, and can be excited by visible light; the compound has good water solubility, can generate a large amount of reactive oxygen species (ROS) under irradiation of green light (520 nm), shows excellent photodynamic activity to tumor cells, and has low toxicity to normal cells.

An imaging system uses a multi-view reflector (MVR) to simultaneously measure the three-dimensional (3D) position and orientation of a light emitter. The MVR is positioned at a back focal plane (BFP) of a light collecting optical system, and uses pyramid reflectors and / or conical reflectors to reflect light in at least two regions of the BFP to at least two detection channels of a detector. The at least two detection channels produce images that are used to determine the 3D position and orientation of a light emitter. The system may be used, for example, to image the structure of molecules or organelles in a cell, track the movement of molecules in a cell, or to study the interaction of molecules within a cell.

The invention discloses engineering mitochondria enriched with alpha2delta2 protein, the expression level of the alpha2delta2 protein in the mitochondria is higher than that of natural mitochondria, the expression quantity of the alpha2delta2 protein is 15-40 times that of the natural mitochondria, and the alpha2delta2 protein is enriched in the outer membrane of the mitochondria. The invention also discloses a preparation method of the engineered mitochondria enriched with the alpha2delta2 protein. The invention also discloses an application of the engineered mitochondria enriched with the alpha2delta2 protein in preparation of medicines for preventing and / or treating fibrosis diseases. In-vitro experiments prove that the engineered mitochondria can significantly enhance the ability of inhibiting fibroblast activation and collagen secretion, and the effect of the engineered mitochondria is far better than that of common mitochondria. The invention provides a brand-new and effective organelle level treatment scheme for solving the clinical problem of fibrosis.

The invention discloses an Apopsin3 mutant and a method for regulating an environment in a plant cell to be acidic, and belongs to the technical field of pH regulation in the plant cell. The regulation and control method comprises the following steps: mutating 83rd and 120th amino acids of the light-operated hydrogen ion pump protein Apopsin3, converting into hydrogen ion channels, expressing the hydrogen ion channels on a cell membrane in a targeting manner, and stimulating with green light of 40-500 [mu] W / mm < 2 > and 520-560 nm so as to regulate the pH in the cell to be acidic. By adopting the cell specific promoter and performing site-specific mutagenesis modification on the specific hydrogen ion pump, accurate pH regulation and control on specific cells or organelles can be realized. An Apopsin3 (FARH) gene is fused with a cell membrane targeting sequence, and under the stimulation of specific light, only the pH of cytoplasm is obviously changed, and the pH of other cell regions is not influenced.

The invention relates to the technical field of organelle separation, and provides a method for rapid purification of peroxisome and oxidation regulation analysis of ultra-long chain fatty acid. The method comprises the following steps: introducing a nucleic acid sequence for coding a fusion protein into a target cell to obtain a cell material, the fusion protein comprising a truncated sequence derived from a peroxisome membrane protein and a tag sequence for affinity purification; taking the cell material as a sample, and separating the peroxisome with the fusion protein from the cell by utilizing an affinity ligand on the basis of a high affinity binding principle. Therefore, according to the method disclosed by the invention, the peroxisome can be rapidly and efficiently enriched, and the whole purification process can be completed within 30 minutes, so that the experimental period is greatly shortened; core protein components of target organelles can be efficiently reserved, and the metabolic activity of peroxisome can be maintained through a complete membrane structure and an internal environment; meanwhile, the same purified sample can synchronously support proteome and metabolome analysis, and direct association of'protein-metabolism 'data is realized.

1. The name of the design product: the main body of a biological particle separator with a transparent membrane layer. 2. The use of the design product: the design product is the main body of a biological particle separator with a transparent membrane layer, which is a device or system for separating specific biological particles (such as cells, organelles, proteins, nucleic acids, viruses, etc.) from complex mixtures. 3. The design points of the design product: the shape shown by solid lines in the view. 4. The picture or photo that best indicates the design points: Design 1 perspective view 1. 5. Design 1 is designated as the basic design. 6. Other circumstances that need to be explained: the part represented by solid lines in the view is the design for which protection is sought, and the part represented by dashed lines does not constitute the design content for which protection is sought. The circular membrane marked by letters A-F in the view is transparent.

The present invention discloses a method for constructing giant organelles in Escherichia coli to enhance the oxygen resistance of [Fe-Fe] hydrogenase, and belongs to the field of enzyme catalysis technology. The method comprises the following steps: constructing two plasmids capable of expressing [Fe-Fe] hydrogenase and guide protein and [Fe-Fe] hydrogenase maturation-promoting protein in Escherichia coli; transforming the two plasmids into Escherichia coli BL21 (DE3) competent cells and performing pre-culture; transferring the pre-culture to a new culture medium, performing aerobic culture, and after culturing for a period of time, determining its OD600 value, transferring the culture to an anaerobic glove box, anaerobically culturing, centrifuging, and obtaining Escherichia coli containing giant organelles. The present invention provides a method for constructing giant organelles in Escherichia coli to enhance the oxygen resistance of [Fe-Fe] hydrogenase, which effectively enhances the oxygen resistance and stability of [Fe-Fe] hydrogenase by constructing giant organelles in Escherichia coli, and provides a new sustainable method for the production of hydrogen.

The utility model relates to a kind of cell vitrification freezing automatic systems, including workbench;Including several injection pumps, the injection pump is installed on workbench, movable piston rod is provided in the injection pump, and the piston rod upper end is connected with driving assembly;Including fluid collecting valve, the fluid collecting valve is installed on workbench, and the fluid collecting valve is connected with the liquid outlet end of injection pump;Including frozen macaroni, the frozen macaroni and fluid collecting valve are connected;Including temperature control board, the temperature control board is installed on workbench, the utility model is advantageous in that system automatically controls the loading and unloading of cryoprotectant, to controllable mode adjustment exchange in oocyte water and cryoprotectant, reduce the pressure or organelle and intracellular membrane system damage of cell structure of osmotic pressure shock to cell, improve the survival rate of cell.

Preparations and formulations capable of crossing and incorporating into a membrane of a cell or an organelle or an exosome are described. Methods of treatments utilizing the preparations and formulations are also described.

The present application relates to the technical field of molecular biology experiment, especially to a kind of plant organelle RNA end analysis method and its application.The present application provides a kind of plant organelle RNA end analysis method, comprising the following steps: RNA is treated with 5' polyphosphatase, in vitro cyclization, then double-end sequencing is carried out, filtering, to obtain clean read length;MeCi algorithm is used to identify clean read length;According to the identification result and the number of clean read length, the 5' and 3' end position of RNA, the end type, the end position of mRNA and ncRNA and the 5' end type of mRNA and ncRNA are judged.The method not only analyzes organelle RNA end modification and organelle RNA poly(A) length and source, but also can be used for screening the substrate RNA of RNase and RBP involved in plant organelle RNA end processing and stability.

This invention relates to the field of organic small molecule fluorescent probe technology, specifically to a neuron-specific dual-targeting fluorescent probe targeting the AChR of cholinergic neurons and its applications. The fluorescent probe, whose structure follows general formula I, is characterized by its dual-targeting structure, making it particularly suitable for the identification and labeling of cholinergic neurons. Furthermore, this fluorescent probe exhibits significant advantages in multiple application scenarios, such as cell imaging, protein and organelle labeling, tumor-specific recognition, and photodynamic therapy, with its potential application value in neuroimaging being particularly prominent. This fluorescent probe is expected to advance the development of neurobiology, disease diagnosis, and treatment methods, and has promising application prospects.

The invention discloses a preparation method and application of near-infrared carbon dots capable of efficiently generating active oxygen and having targeting capacity, and belongs to the technical field of carbon nanomaterials. Compared with conventional blue-green light carbon dots, the near-infrared carbon dot provided by the invention has the advantages of large tissue penetration depth, small self-luminous interference of organisms, small damage to tissues and the like. The carbon dots provided by the invention can efficiently generate singlet oxygen, and have the capability of photodynamically killing tumor cells. Besides, abundant functional groups on the surfaces of the carbon dots are utilized, molecules capable of targeting organelles are modified on the surfaces of the carbon dots, so that the carbon dots can accurately position and target mitochondria, and compared with carbon dots without targeting capability, the carbon dots have stronger killing power on cancer cells.

The invention belongs to the technical field of bioelectricity medicine and cell therapy, and particularly relates to a system for carrying out intracellular electric treatment based on ultrahigh-frequency ultrashort pulses and application of the system. The system comprises a high-voltage ultrashort pulse generation module, a control module and an adherent cell processing module, wherein the high-voltage ultrashort pulse generation module is respectively connected with the control module and the adherent cell processing module; the control module presets a plurality of pulse parameter combinations, the high-voltage ultrashort pulse generation module outputs a pulse electric field with preset parameters according to an instruction sent by the control module, and the pulse electric field acts on an organelle through the adherent cell processing module. High-frequency ultrashort pulses are adopted, the unique electric field distribution characteristic of the high-frequency ultrashort pulses can penetrate through cell membranes without causing permanent damage, and the high-frequency ultrashort pulses directly act on specific organelles such as mitochondria, endoplasmic reticulum and lysosome. And the system can flexibly induce four main programmed cell death modes, is suitable for different treatment requirements, and is beneficial to precise medical treatment.

According to the selective organ targeting endoplasmic omentum fusion nano delivery platform and the application thereof, the platform realizes bidirectional regulation and control of organ targeting selectivity and cell membrane fusion efficiency through reasonable design of components and proportions on the basis of the synergistic effect of functional lipid combination, and the delivery efficiency of the endoplasmic omentum fusion nano delivery platform is improved. The problems that the organ specificity accumulation of a traditional delivery system for delivering drugs is insufficient, the release efficiency of intracellular drugs after delivery is low and the accumulation of organelles is limited are solved, and the drugs are delivered to specific organs in a targeted manner and are highly fused with endoplasmic reticulum membranes of cells in the organs; the method can be widely applied to the treatment of organ specificity and endoplasmic reticulum related diseases, such as lung intracellular bacterial infection treatment, pulmonary fibrosis treatment, anti-tumor vaccine construction and the like. The raw materials for construction of the platform are easy to obtain, and the platform is prepared by adopting a scalable simple process and has a good clinical transformation prospect.

Compositions and methods disclosed herein relate to improved in vitro cell-free systems for various biological reactions. In one aspect, a composition of the present disclosure includes: a cell-free extract derived from an organism; a nucleic acid; and an organelle that is heterologous to the organism.

Described herein are methods, compositions, and kits for isolation of mitochondria from cryopreserved primary cells (e.g., fibroblast cells, mesenchymal stromal / stem cells) to be used in mitochondrial organelle transplantation (MOT) methods and compositions.

A chip 10 comprising a microfluidic space 12 accommodating a plurality of micro-entities in a substantially planar array and a dispense region 16 configured to provide a flow of carrier fluid non-parallel to an exit orifice 14, wherein the microfluidic space has the height of a single layer of micro-entities and contains a carrier fluid in which the micro-entities are manipulated using a force. The exit orifice 14 may be double or five times the height of the microfluidic space and may comprise a single gap, a series of gaps, a sieve or a semi-permeable membrane. The carrier fluid may be provided at less than 70, or 60-70 degrees to the orifice. The chip may comprise carrier fluid inlets 26, optionally symmetrically positioned either side of an outlet 24. A cavity in the dispense region may have a depth of 100-200 µm. The micro-entities may be a cell, part of a cell, a bead, an organelle, an organism, a liposome, a biosynthetic structure or a vesicle, and may be localised in a sequestration pen. One or more micro-entities may be encapsulated in at least a subset of the microdroplets 18. The force may be electrowetting, EWOD or oEWOD.

Processes and compositions are described for preparing new, colloidally stable, coated nanomagnetic particles useful for both in-vitro and in-vivo biomedical applications, including cell targeting and capturing cells, microorganisms, and cellular organelles or entities such as exosomes. These nanomagnetic particles can also be used as imaging contrast agents due to their small size and high magnetic moment. The nanomagnetic particles include a series of sequentially added, stabilizing surface coatings rendered onto nano-sized magnetic crystal clusters (e.g., magnetite particles) to impart colloidal stability in complex biological samples with minimal leaching of the coating materials, high binding capacity, and low non-specific binding. Another benefit of this invention is the ability to utilize both external and internal magnetic field-generating separation devices to effect separation of the magnetic nanoparticles.

The present invention relates to a bacterial toxin variant and use thereof. According to an aspect, a variant, a fusion protein, a polypeptide thereof, and a base editing system including same facilitate binding with Cas proteins because the polypeptide size is small, and compared to an existing base editing system using Ssda proteins, base editing efficacy is significantly improved, enabling more effective base editing, and base editing is also possible in cell organelles.

The present invention relates to a DNA base editing composition including uracil DNA glycosylase (UDG), and a DNA base editing method using same. Specifically, the present invention relates to a DNA base editing composition including a DNA binding protein, cytosine deaminase, adenine deaminase, and UDG, or polynucleotides encoding the proteins, or a DNA base editing method using same. The present invention is useful for correcting bases in nuclear DNA or organellar DNA and particularly for correcting bases in DNA of organelles such as chloroplasts or mitochondria. In the present invention, the UDG is present independently without being fused to the DNA binding protein, the cytosine deaminase, and / or the adenine deaminase.