128 results about "Organelle" patented technology

The present invention concerns systems, materials, and methods of cell, cell organelle and / or particle separation and / or concentration utilizing magnetic levitation to affect separation. In some embodiments, the separation and / or concentration is performed in the wells of a multi-well plate.

The invention discloses a preparation method and application of a mitochondrial targeting polypeptide modified carbon quantum dot probe, and relates to the technical field of nano biological materials and fluorescence imaging.The preparation method comprises the steps that citric acid and urea are dissolved in a dimethyl sulfoxide solution, mixed, heated, filtered, added with ethyl alcohol, centrifuged and freeze-dried, and carbon quantum dots are obtained; adding an MES buffer solution and an activating agent, reacting the polypeptide at room temperature, heating, reacting, centrifuging, dialyzing and freeze-drying to obtain polypeptide modified carbon quantum dots; the carbon quantum dots are synthesized from bottom to top, and the preparation method is simple. After polypeptide modification, the product has an obvious mitochondrial targeting effect, and the product is good in biocompatibility, so that the product can be used for imaging and dynamic tracking of living organelles. The probe developed by the invention shows obvious application potential in key cell events such as mitochondrial dynamic monitoring and the like.

The invention discloses a terahertz near-field system sample nanometer repeated positioning method, and belongs to the technical field of terahertz near-field imaging. According to the method, repeated positioning with nanoscale precision is realized through two key technical means: displacement compensation: after first scanning of a sample area is completed and reference coordinates are recorded, second scanning is carried out, and the offset of the center coordinates of a probe relative to the reference coordinates in the X-axis and / or Y-axis direction is monitored; before follow-up scanning, the reverse value of the offset is used as the compensation amount to be input into a control system so as to correct the reference coordinate; and voltage control: monitoring and controlling the voltage of the system in real time when the probe withdrawing operation is intermittently executed in the current scanning and the next scanning, and stopping probe withdrawing within one second when the voltage value rises to 180V so as to control the withdrawing distance of the probe. According to the method, the repeated positioning accuracy can be controlled within 5 nanometers, and the method is suitable for multiple times of accurate imaging of biological organelles and nano structures in semiconductors.

The invention discloses a high-activity BODIPY type photosensitizer targeting double organelles as well as a preparation method and application of the high-activity BODIPY type photosensitizer, and relates to the technical field of medical photosensitizers. The high-activity BODIPY type photosensitizer is prepared by the following steps: reacting 2, 4-dimethyl pyrrole with 4-bromobutyryl chloride to obtain a compound Br-BY containing carboxyl, then reacting with morpholine to obtain a morpholine substituted compound Mor-BY, and finally reacting with N-iodosuccinimide (NIS) to obtain a single atom I substituted BODIPY compound Mor-IBY, namely the target photosensitizer. The photosensitizer prepared by the invention can target mitochondria and lysosome in cancer cells at the same time, and can be excited by visible light; the compound has good water solubility, can generate a large amount of reactive oxygen species (ROS) under irradiation of green light (520 nm), shows excellent photodynamic activity to tumor cells, and has low toxicity to normal cells.

An imaging system uses a multi-view reflector (MVR) to simultaneously measure the three-dimensional (3D) position and orientation of a light emitter. The MVR is positioned at a back focal plane (BFP) of a light collecting optical system, and uses pyramid reflectors and / or conical reflectors to reflect light in at least two regions of the BFP to at least two detection channels of a detector. The at least two detection channels produce images that are used to determine the 3D position and orientation of a light emitter. The system may be used, for example, to image the structure of molecules or organelles in a cell, track the movement of molecules in a cell, or to study the interaction of molecules within a cell.

The invention discloses engineering mitochondria enriched with alpha2delta2 protein, the expression level of the alpha2delta2 protein in the mitochondria is higher than that of natural mitochondria, the expression quantity of the alpha2delta2 protein is 15-40 times that of the natural mitochondria, and the alpha2delta2 protein is enriched in the outer membrane of the mitochondria. The invention also discloses a preparation method of the engineered mitochondria enriched with the alpha2delta2 protein. The invention also discloses an application of the engineered mitochondria enriched with the alpha2delta2 protein in preparation of medicines for preventing and / or treating fibrosis diseases. In-vitro experiments prove that the engineered mitochondria can significantly enhance the ability of inhibiting fibroblast activation and collagen secretion, and the effect of the engineered mitochondria is far better than that of common mitochondria. The invention provides a brand-new and effective organelle level treatment scheme for solving the clinical problem of fibrosis.

The invention relates to the technical field of organelle separation, and provides a method for rapid purification of peroxisome and oxidation regulation analysis of ultra-long chain fatty acid. The method comprises the following steps: introducing a nucleic acid sequence for coding a fusion protein into a target cell to obtain a cell material, the fusion protein comprising a truncated sequence derived from a peroxisome membrane protein and a tag sequence for affinity purification; taking the cell material as a sample, and separating the peroxisome with the fusion protein from the cell by utilizing an affinity ligand on the basis of a high affinity binding principle. Therefore, according to the method disclosed by the invention, the peroxisome can be rapidly and efficiently enriched, and the whole purification process can be completed within 30 minutes, so that the experimental period is greatly shortened; core protein components of target organelles can be efficiently reserved, and the metabolic activity of peroxisome can be maintained through a complete membrane structure and an internal environment; meanwhile, the same purified sample can synchronously support proteome and metabolome analysis, and direct association of'protein-metabolism 'data is realized.

1. The name of the design product: the main body of a biological particle separator with a transparent membrane layer. 2. The use of the design product: the design product is the main body of a biological particle separator with a transparent membrane layer, which is a device or system for separating specific biological particles (such as cells, organelles, proteins, nucleic acids, viruses, etc.) from complex mixtures. 3. The design points of the design product: the shape shown by solid lines in the view. 4. The picture or photo that best indicates the design points: Design 1 perspective view 1. 5. Design 1 is designated as the basic design. 6. Other circumstances that need to be explained: the part represented by solid lines in the view is the design for which protection is sought, and the part represented by dashed lines does not constitute the design content for which protection is sought. The circular membrane marked by letters A-F in the view is transparent.

The utility model relates to a kind of cell vitrification freezing automatic systems, including workbench;Including several injection pumps, the injection pump is installed on workbench, movable piston rod is provided in the injection pump, and the piston rod upper end is connected with driving assembly;Including fluid collecting valve, the fluid collecting valve is installed on workbench, and the fluid collecting valve is connected with the liquid outlet end of injection pump;Including frozen macaroni, the frozen macaroni and fluid collecting valve are connected;Including temperature control board, the temperature control board is installed on workbench, the utility model is advantageous in that system automatically controls the loading and unloading of cryoprotectant, to controllable mode adjustment exchange in oocyte water and cryoprotectant, reduce the pressure or organelle and intracellular membrane system damage of cell structure of osmotic pressure shock to cell, improve the survival rate of cell.

The present application relates to the technical field of molecular biology experiment, especially to a kind of plant organelle RNA end analysis method and its application.The present application provides a kind of plant organelle RNA end analysis method, comprising the following steps: RNA is treated with 5' polyphosphatase, in vitro cyclization, then double-end sequencing is carried out, filtering, to obtain clean read length;MeCi algorithm is used to identify clean read length;According to the identification result and the number of clean read length, the 5' and 3' end position of RNA, the end type, the end position of mRNA and ncRNA and the 5' end type of mRNA and ncRNA are judged.The method not only analyzes organelle RNA end modification and organelle RNA poly(A) length and source, but also can be used for screening the substrate RNA of RNase and RBP involved in plant organelle RNA end processing and stability.

This invention relates to the field of organic small molecule fluorescent probe technology, specifically to a neuron-specific dual-targeting fluorescent probe targeting the AChR of cholinergic neurons and its applications. The fluorescent probe, whose structure follows general formula I, is characterized by its dual-targeting structure, making it particularly suitable for the identification and labeling of cholinergic neurons. Furthermore, this fluorescent probe exhibits significant advantages in multiple application scenarios, such as cell imaging, protein and organelle labeling, tumor-specific recognition, and photodynamic therapy, with its potential application value in neuroimaging being particularly prominent. This fluorescent probe is expected to advance the development of neurobiology, disease diagnosis, and treatment methods, and has promising application prospects.

The invention belongs to the technical field of bioelectricity medicine and cell therapy, and particularly relates to a system for carrying out intracellular electric treatment based on ultrahigh-frequency ultrashort pulses and application of the system. The system comprises a high-voltage ultrashort pulse generation module, a control module and an adherent cell processing module, wherein the high-voltage ultrashort pulse generation module is respectively connected with the control module and the adherent cell processing module; the control module presets a plurality of pulse parameter combinations, the high-voltage ultrashort pulse generation module outputs a pulse electric field with preset parameters according to an instruction sent by the control module, and the pulse electric field acts on an organelle through the adherent cell processing module. High-frequency ultrashort pulses are adopted, the unique electric field distribution characteristic of the high-frequency ultrashort pulses can penetrate through cell membranes without causing permanent damage, and the high-frequency ultrashort pulses directly act on specific organelles such as mitochondria, endoplasmic reticulum and lysosome. And the system can flexibly induce four main programmed cell death modes, is suitable for different treatment requirements, and is beneficial to precise medical treatment.

According to the selective organ targeting endoplasmic omentum fusion nano delivery platform and the application thereof, the platform realizes bidirectional regulation and control of organ targeting selectivity and cell membrane fusion efficiency through reasonable design of components and proportions on the basis of the synergistic effect of functional lipid combination, and the delivery efficiency of the endoplasmic omentum fusion nano delivery platform is improved. The problems that the organ specificity accumulation of a traditional delivery system for delivering drugs is insufficient, the release efficiency of intracellular drugs after delivery is low and the accumulation of organelles is limited are solved, and the drugs are delivered to specific organs in a targeted manner and are highly fused with endoplasmic reticulum membranes of cells in the organs; the method can be widely applied to the treatment of organ specificity and endoplasmic reticulum related diseases, such as lung intracellular bacterial infection treatment, pulmonary fibrosis treatment, anti-tumor vaccine construction and the like. The raw materials for construction of the platform are easy to obtain, and the platform is prepared by adopting a scalable simple process and has a good clinical transformation prospect.

Compositions and methods disclosed herein relate to improved in vitro cell-free systems for various biological reactions. In one aspect, a composition of the present disclosure includes: a cell-free extract derived from an organism; a nucleic acid; and an organelle that is heterologous to the organism.

The present invention relates to a DNA base editing composition including uracil DNA glycosylase (UDG), and a DNA base editing method using same. Specifically, the present invention relates to a DNA base editing composition including a DNA binding protein, cytosine deaminase, adenine deaminase, and UDG, or polynucleotides encoding the proteins, or a DNA base editing method using same. The present invention is useful for correcting bases in nuclear DNA or organellar DNA and particularly for correcting bases in DNA of organelles such as chloroplasts or mitochondria. In the present invention, the UDG is present independently without being fused to the DNA binding protein, the cytosine deaminase, and / or the adenine deaminase.

The invention relates to the technical field of biology, in particular to an SERS probe for Mfn2 detection, a preparation method of the SERS probe, an SERS sensor and application of the SERS sensor. Based on a surface enhanced Raman spectroscopy (SERS) technology, a gold nanoparticle SERS probe coated with an antibody 1 and a silver nanoparticle SERS probe coated with an antibody 2 are constructed, a sandwich structure is formed through combination of the antibody 1, an antigen and the antibody 2, the structure effectively improves SERS hot spot distribution and Mfn2 detection sensitivity, and traditional measurement steps are simplified. The constructed biologically coded SERS probe can be used for measuring Mfn2 with different concentrations. Based on the prepared SERS sensor, the dynamic measurement of the expression of the single living cell Mfn2 in the process of electrical stimulation of dental pulp stem cell odontogenic differentiation is realized, and the SERS sensor has wide clinical application prospect and transformation value in the aspects of measurement of protein molecules in a single cell organelle and diagnosis of Mfn2 related diseases.

This invention discloses the application of lipoic acid analog formulations in the preparation of anti-cardiomyocyte senescence products. The lipoic acid analog DMAE-LA is N-(2-(dimethylamino)ethyl)-5-(1,2-dithiopentane-3-yl)pentanamide, with a concentration of 2-25 μM. DMAE-LA pretreatment effectively reduces intracellular ROS, improves mitochondrial function, inhibits the overexpression of p21 and HMGB1, and restores Lamin B1 levels, thereby alleviating the senescent manifestations of cardiomyocytes. DMAE-LA reduces H₂S oxidative depletion by inhibiting ROS generation and may promote H₂S synthase activity, increasing endogenous H₂S levels and further enhancing cellular antioxidant capacity. In summary, DMAE-LA can significantly inhibit cardiomyocyte senescence through multiple antioxidant mechanisms and organelle function protection, providing a potential intervention strategy for targeting cellular senescence in cardiovascular diseases.

The invention discloses a nutrient signal substance synergistic controlled-release fertilizer for promoting efficient absorption of phosphorus in rice, and belongs to the technical field of controlled-release fertilizer production. Three technical breakthroughs are realized by constructing a'microbial response outer membrane layer-enzyme response inner membrane layer 'dual biological response controlled release system and cooperating with an'organelle targeted phytic acid synthesis signal guide chain substance': firstly, phosphorus release is synchronized with a rhizosphere activity period through the microbial response characteristic of an outer membrane; secondly, accurate release of signal substances in the filling stage is ensured through an inner layer film laccase triggering mechanism; 3, an endogenous signal channel of the plant is activated by utilizing an IP3 analogue precursor molecule, so that the synthesis of phytic acid is enhanced from a metabolism level, and a'grain bank strong 'is created to pull the absorption and transport of phosphorus, thereby systematically improving the utilization efficiency of the phosphate fertilizer and realizing normal form transformation from'passive supply' to'active demand '.

Protein and RNA-based condensates are emerging as an alternative to classical membrane-bound organelles for the task of compartmentalizing molecules and biochemical reactions. We describe methods for making RNA condensates in mammalian cells. We further show that aptamers make it possible to recruit peptides and proteins to the condensates with high specificity. Such RNA condensates can be modularly customized and offer a route toward creating systems of functional artificial organelles.

The invention provides a fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of biological analysis and detection. The fluorescent probe disclosed by the invention has a structure as shown in a formula I; the invention also provides a preparation method of the fluorescent probe and application of the fluorescent probe in cell activity detection or preparation of a cell activity detection kit. The fluorescent probe provided by the invention can reflect the cell state by migrating organelles, realizes dual-channel imaging, and can meet the actual requirements of cell activity detection.

The present invention relates to base editing using dead uracil DNA glycosylase (dUDG). The present invention is useful for C-to-T base editing in nuclear DNA or organelle DNA, and in particular, is useful for C-to-T base editing in organelle DNA such as chloroplasts or mitochondria. The present invention also relates to a UDG variant and a novel DNA base editing use thereof.

The embodiment of the invention discloses a microscopic image video stream identification method and device, a storage medium and a program product, and relates to the technical field of data processing. The method comprises the following steps: acquiring a first microscopic image video stream, performing organelle identification on each microscopic image in the first microscopic image video stream to obtain attribute information of each organelle, and converting the attribute information into structured information; respectively adding each piece of attribute information into the first microscopic image video stream to obtain a second microscopic image video stream; and generating an analysis report matched with the first microscopic image video stream based on the structured information and the second microscopic image video stream. According to the scheme provided by the embodiment of the invention, the microscopic image video stream can be quickly and accurately identified, the analysis report is generated, and the labor cost is greatly reduced.

The invention relates to the technical field of super-resolution optical imaging, in particular to a super-resolution imaging probe of GQDs-double fluorescent dye based on an FRET effect. The probe takes graphene quantum dots as donors and double fluorescent dyes as receptors, the surfaces of the graphene quantum dots are modified with polyethylene glycol, and the probe is used for super-resolution optical imaging of specific organelles through antibody specific targeting. According to the super-resolution imaging probe of the GQDs-double fluorescent dye based on the FRET effect, provided by the invention, single-laser excited double-channel super-resolution optical imaging is realized, and the super-resolution imaging probe can be used for super-resolution optical imaging based on a single-molecule positioning method.

The invention discloses a stem cell-based microvesicle as well as a preparation method and application thereof, belongs to the technical field of biological tissue engineering, and particularly relates to a preparation method of the stem cell-based microvesicle, which comprises the following steps: culturing stem cells until the cell fusion degree is 80-90%, performing hypoxia treatment, and cleaning for 2-3 times; standing and culturing the cleaned stem cells, then adding a calcium ion carrier, and carrying out ultrasonic treatment; the serum-free basic culture medium containing the calcium ion carrier is immediately absorbed and removed, the cells are cleaned through PBS, culture is conducted for 24 h, and cell supernatant is collected and merged respectively; performing first centrifugation to remove cell debris; performing second centrifugation to remove dead cells and large cell debris; carrying out third centrifugation to remove organelles; fourth centrifugation is carried out, large vesicles are removed, and a final supernatant is obtained; and filtering and centrifuging to obtain a microvesicle precipitate. The preparation method of the microvesicles aims at systematically improving the yield and biological activity of the stem cell microvesicles through low-oxygen pre-stimulation, ultrasonic-calcium ion carrier synergistic induction and differential centrifugation.

The invention discloses application of oxygen-carrying protein VHb in construction of high-yield astaxanthin engineering bacteria, and belongs to the technical field of biology. According to the method, carRPY27R, carB, fusion genes crtW-crtZ and GGPPS are iteratively integrated onto a yarrowia lipolytica chassis genome by utilizing a synthetic biological technology, so that two recombinant strains for producing astaxanthin are obtained. On the basis, the oxygen-carrying protein VHb from vitreoscilla is expressed, and compared with the oxygen-carrying protein VHb which is not expressed, the VHb is positioned to the endoplasmic reticulum, so that the accumulation level of astaxanthin is improved by 272.99%, and the biomass is improved by 94.15%. According to the method, the oxygen-carrying protein VHb is expressed on cytoplasm and organelle, so that the synthesis efficiency and biomass of astaxanthin are remarkably improved. In addition, the intracellular dissolved oxygen level is improved, so that the cost for improving the dissolved oxygen in the fermentation process is reduced.

The invention belongs to the technical field of biology, and particularly relates to an enzyme co-localization system and a construction method and application thereof. The invention relates to an enzyme co-localization system. The system contains a protein of a WS / DGAT family for constructing a lipid droplet organelle and a target enzyme fragment connected with an anchoring protein specifically targeting the lipid droplet organelle. The protein of the WS / DGAT family is an enzyme tDGAT of the WS / DGAT family, the base sequence of the enzyme tDGAT is as shown in SEQ ID NO.1, and the coding amino acid sequence of the enzyme tDGAT is as shown in SEQ ID NO.2. According to the invention, through the characteristics that lipid droplets (LD) have ubiquitous cellular structures and proteins can be positioned on LDs of different organisms in a cross-species manner, plasmids are utilized to heterologously express an enzyme tDGAT belonging to a WS / DGAT family in escherichia coli C43 (DE3), so that more stable lipid droplet organelles can be formed in prokaryotic escherichia coli; meanwhile, plasmids are used for expressing protein capable of being specifically combined with the LD surface, the N end of the protein is fused with biosynthetic pathway enzyme to form recombinant protein, then expression conditions are optimized, and local enzyme concentration is improved.

The invention discloses an application of PINK1 in regulation and control of ER positive breast cancer tamoxifen drug resistance. The invention relates to application of PINK1 as a biomarker in evaluating the tamoxifen resistance of ER positive breast cancer cells. The drug resistance is evaluated by detecting the expression level of PINK1 in a sample and combining mitochondrial autophagy activity. The expression level of the PINK1 comprises an mRNA level and / or a protein level. According to the invention, the drug resistance formation process is systematically analyzed from the perspective of quality control of an organelle of mitochondrial autophagy, and through public database analysis and experimental verification, the overall up-regulation of the mitochondrial autophagy pathway in drug-resistant cells is clearly revealed, and the drug-resistant phenotype is promoted by maintaining the mitochondrial steady state, thereby opening up a new direction for understanding the drug-resistant mechanism.

This invention provides a benzothiadiazole-based dual-target viscosity-sensitive fluorescent probe for lipid droplets and lysosomes, its preparation method, and its application, relating to the field of fluorescent probes. The preparation method of the benzothiadiazole-based dual-target viscosity-sensitive fluorescent probe includes the following steps: a primary reaction and a secondary reaction. The benzothiadiazole-based dual-target viscosity-sensitive fluorescent probe of this invention can simultaneously target and monitor two organelles (lipid droplets and lysosomes), and exhibits low cytotoxicity and accurate cell localization in HeLa cell imaging experiments; furthermore, it achieves both good polarity response and effective response to intracellular viscosity, demonstrating excellent response performance.

The utility model provides a seminal fluid treatment device which comprises a base, a seminal fluid injection port arranged at the first end of the base, a sperm recovery pool arranged at the second end of the base, a seminal fluid storage bin and a sperm upstream channel, the seminal fluid storage bin and the sperm upstream channel are arranged in the base, and the first end of the seminal fluid storage bin is communicated with the seminal fluid injection port. The second end of the semen storage bin is communicated with the first end of the sperm upstream channel, the second end of the sperm upstream channel is communicated with the sperm recovery pool, and the first end and the second end of the sperm upstream channel are both provided with filter membranes. According to the utility model, on the basis of the characteristic that sperms swim upwards, centrifugal operation is not needed in the semen treatment process, so that the damage to sperm DNA and organelles is reduced to the greatest extent; the operation process is simple and rapid, the technical requirements for operators are low, and standardization is easier; in addition, less culture medium is needed in the operation process, a centrifugal machine and the like do not need to be purchased, and the use cost is low.