346 results about "Organelle" patented technology

The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.

The current invention provides a biocompatible energy recharge system to normalize body functions for a mammal. Both stationary and circulatory scalar wave energy was produced and utilized to enable the entire body of a mammal to increase the energy potential of any cell in the body, to promote cell growth, to facilitate cell repair, and to reduce cell destruction, hence the cells, organs, systems and the entire body of a mammal become healthy, and to treat a disease or disorder. The invention is a combination of the generators of the three-dimensional bio-waves in the stationary and in the circulationary formats for empowering the cells in all parts of a mammal, and a method of using the 3D bio-waves uniformly to empower each organ of a mammal to restore and maintain the normal body health and function.

The invention relates to the technical field of plant exosome-like extracts, in particular to a liquorice exosome-like extract as well as extraction and application thereof. The method comprises the following steps: firstly, removing impurities such as cells, cell debris, organelles, large vesicles and the like in homogenate by adopting a combination mode of low-speed centrifugation and medium-speed centrifugation, and further purifying the homogenate by adopting high-speed centrifugation after the low-speed centrifugation and the medium-speed centrifugation are completed, so as to obtain the exosome-like extract. And the setting of the increasing rotation speed is beneficial to finer separation of exosome-like particles with different sizes and densities, so that the purity and quality of the extract are improved. According to the method, all centrifugal operations are carried out in a low-temperature environment of 4 DEG C; the low-temperature condition is beneficial to maintaining the biological activity and stability of the sample, and the problems of protein denaturation, enzyme inactivation and the like caused by temperature rise are reduced, so that the quality and performance of the exosome-like extract are ensured.

Methods of labelling one or more subcellular components (e.g., an organelle and / or subcellular region) in vivo are provided. Methods of labelling a protein in vivo are provided. Methods of determining a nucleic acid sequence in situ are also provided.

The invention relates to the technical field of paperboard products, and discloses an environment-friendly mildew-proof and antibacterial corrugated paperboard and a preparation method thereof. The quaternary ammonium salt substance of the modified terminal isocyanate polyurethane can attack the cell membrane or cell wall of microorganisms, so that the cell membrane function is affected and irreversible damage is caused to the structure of the microorganism organelles. The chemical element S is contained in the structure and has strong oxidizing and reducing properties, so that the cell membrane of microorganisms can be destroyed, organic matter can be decomposed, and the metabolism and growth environment of molds can be destroyed. The epoxy group and isocyanate group of the modified terminal isocyanate polyurethane can react with the hydroxyl group and carboxyl group on the surface of oxidized cassava starch, and at the same time, the dimethylol urea and oligomers in the urea-formaldehyde resin undergo cross-linking reaction with the hydroxyl group in the starch molecules to form a cross-linked network structure, so that water molecules are prevented from penetrating into the paper, thereby improving the bonding strength and water resistance of the corrugated paperboard.

Methods of labelling one or more subcellular components (e.g., an organelle and / or subcellular region) in vivo are provided. Methods of labelling a protein in vivo are provided. Methods of determining a nucleic acid sequence in situ are also provided.

The invention discloses an organelle combination phenotype high-throughput drug screening method based on machine learning, and relates to the technical field of drug research and development biology. The method comprises the following steps of: 1, analyzing a cell image by ComOrganNet; step 2, performance evaluation of the ComOrganNet model is carried out; 3, the performance of ComOrganNet in the data set is expanded; step 4, comparing the ComOrganNet with a conventional cell parameter extraction method; 5, verifying the practicability of the ComOrganNet classification model; 6, establishing a natural product library and obtaining an active component screening result; according to the method, cell image analysis and preprocessing are carried out in combination with ComOrganNet, deep learning classification is carried out in combination with OfficientNet V2, higher classification accuracy can be achieved in high-throughput drug screening, particularly, when multi-channel image input is used, the accuracy of a model in a verification set reaches 80%, and the result is remarkably superior to that of single-channel image training.

The invention discloses an organelle-targeted fluorescent probe for specific detection of tumor cells as well as preparation and application of the organelle-targeted fluorescent probe, and the specific structural formula of the fluorescent probe is as follows: BCY-DP shows obvious specific targeting ability of tumor cell mitochondria and has been applied to ONOO-selective imaging in living cell mitochondria; results show that the BCY-DP has high selectivity on ONOO <-> in tumor cells, can successfully distinguish the ovarian cancer cells from normal ovarian cells, and can be used for diagnosis of cancer cells.

The disclosure provides a method of training a deep learning model on microscopy images of cells that are under the influence of different drugs or affected by different disease conditions and a database atlas to map how different drugs or disease conditions affect cellular morphology such that the drug or disease condition can be predicted from the microscopy image once the atlas has been trained.

The invention relates to the technical field of antitumor drugs, in particular to a Ru (II) complex and a preparation method and application thereof, and the Ru (II) complex has a structure as shown in a formula I. The Ru (II) complex synthesized by the method disclosed by the invention not only has relatively high cytotoxicity, but also has good photodynamic antitumor activity. After the complex is illuminated, the efficiency of the complex entering cells in an active transportation mode can be accelerated, mitochondria and endoplasmic reticulum are targeted at the same time, mitochondrial membrane potential decline and endoplasmic reticulum stress are caused to form a dual organelle damage effect, immunogenic cell death is caused, the tumor microenvironment is adjusted, and the tumor cell immunogenicity is improved. The enrichment of dendritic cells (DCs) in tumor cells is realized, the chemotactic activity of effector T cells is improved, the proportion of CD4 < + > and Foxp3 < + > cell populations is reduced, and finally the anti-tumor immune response is activated. Meanwhile, the complex provided by the invention can also induce the cell to generate pan apoptosis, retard the cell cycle in the G2 / M period and enhance the effect of the complex in inhibiting tumor proliferation.

The method comprises the following steps: fixing with a pre-fixing solution, wrapping and forming with a solid agent, fixing with a fixing solution, embedding with an embedding agent, decomposing melanin with a melanin decomposing solution, pre-decoloring with a pre-decoloring solution, decoloring with a decoloring solution, and eluting with a rinsing solution. The invention provides a melanin removing method capable of effectively avoiding retina RPE layer cell exfoliation, protein denaturation and nucleic acid degradation. The method mainly comprises the following steps: keeping the original state of tissue cells by adopting a pre-fixing solution, keeping the complete tissue structure by adopting a solid agent, decomposing melanin particles by adopting a melanin decomposing solution composed of various extracts, separating melanin by adopting a pre-decoloring solution and a decoloring solution, eluting the melanin decomposed on the surface of the tissue by adopting a rinsing solution, and the like. On the premise that the retina tissue structure is intact and protein, nucleic acid and cell structures on the surface of the tissue section are not damaged, melanin particles in the tissue section are effectively decomposed, the biological activity of various organelles on the surface of the section is kept, and the requirements of subsequent chemical staining of various tissues are met.

The invention belongs to the field of gene engineering, and particularly relates to application of a willow uridine diphosphate glucose-4-epimerase gene SpUGE1 in plant cell wall component remodeling, organelle cadmium content reduction and cadmium resistance improvement. According to the invention, the SpUGE1 gene in willow is extracted, and is transferred into arabidopsis thaliana by constructing a recombinant plasmid, so that the biological activity, Cd < 2 + > concentration and the like of wild arabidopsis thaliana and transgenic arabidopsis thaliana are compared. The result of the embodiment shows that the transgenic arabidopsis thaliana has certain tolerance to cadmium stress, the cell wall of the transgenic arabidopsis thaliana is obviously thickened, and the content of cadmium in organelles is obviously reduced.

The invention belongs to the technical field of molecular biology and economic forest fungal disease control, and particularly relates to a SorcPp-miR compound, a using method and application. The invention provides application of miRNA162 to improvement of plant disease resistance, the nucleotide sequence of the miRNA162 is shown as SEQ ID NO.1, and the miRNA162 can improve the expression quantity of a disease-resistant gene VTE6 and prevent and treat plant diseases. The invention further provides a Sorcpp-miR compound, the Sorcpp-miR compound comprises the cell penetrating peptide and the miRNA162, and the cell penetrating peptide can carry the exogenous miRNA162 to enter plant cells and specifically enter organelles to target downstream anti-disease genes and promote the plant to improve the disease resistance.

The invention discloses a polypeptide drug chimera and a preparation method thereof, and belongs to the technical field of polypeptide drug carriers. The polypeptide drug chimera is constructed by a recognition segment specifically combined with a target protein and a functional polypeptide carrier mediating the target protein to be transferred to lysosome; an antibody is combined with a lysosome signal amino acid sequence, and pathogenic antigen protein is transferred into a lysosome subcellular organelle for degradation through specific combination of the antibody and different antigens.

The invention relates to the technical field of organelle separation, and provides a combination of ATF6 alpha N protein and peroxisome, application of the ATF6 alpha N protein in separation and purification of the peroxisome and a method for separating and purifying the peroxisome. The ATF6 alpha N protein is combined with membrane protein PMP70 and / or ABCD1 of the peroxisome; the method comprises the following steps: combining tagne-ATF6 alpha N fusion protein with a medium to obtain a fusion protein-medium compound; adding a roughly separated peroxisome sample into the fusion protein-medium compound, so as to obtain a fusion protein-medium-peroxisome compound; and adding an elution buffer solution, and collecting the eluent to obtain the peroxisome combined with the fusion protein. The method is easy and convenient to operate, complex instruments and equipment are not needed, the peroxisome can be separated from cells and tissues, and the obtained peroxisome is high in purity and can be used for conventional experiments such as subsequent protein spectrum analysis and flow analysis.

The invention discloses a method for evaluating the environmental risk of perfluorooctane sulfonic acid by using euglena gracilis, and belongs to the field of environmental pollutant biological monitoring and ecotoxicology evaluation. The method comprises the following steps: detecting the photosynthetic activity and the movement ability of Euglena gracilis exposed to perfluorooctane sulfonic acid with environmental concentration and the organelle distribution condition of the perfluorooctane sulfonic acid in euglena gracilis cells in situ; the photosynthetic activity comprises in-situ monitoring of chlorophyll fluorescence parameters and maximum non-photochemical quenching coefficient of euglena gracilis, and quantitative analysis of chloroplast pigment content; the movement ability comprises horizontal light-driving movement speed and vertical light-driving movement speed measurement of the euglena gracilis and flagellum shedding rate calculation. The method breaks through the limitation of a traditional single autotrophic mode, reveals a regulation and control mechanism of nutritional conditions on toxic effects, provides a scientific basis for water environment safety monitoring and optimization, and has the advantages of being convenient to operate and low in cost.

The invention discloses a tellurium-doped carbon quantum dot and a preparation method and application thereof, the tellurium-doped carbon quantum dot is obtained by using 2, 2 '-diaminodiphenyl telluride as a precursor, the average particle size is 1.9 nm, the thickness is about 2.1 nm, and the surface is rich in amino groups. And fixing (4-carboxybutyl) triphenylphosphonium bromide, 3-morpholinopropionic acid or 3-((4-methylphenyl) sulfonamide) propionic acid on the surfaces of the tellurium-doped carbon quantum dots to prepare Mito-Te-CDs, Lyso-Te-CDs and ER-Te-CDs with an organelle targeting function. The Mito-Te-CDs, the Lyso-Te-CDs and the ER-Te-CDs are good in biocompatibility, can be used as fluorescent probes for targeted imaging of organelles, can be used for recognition and removal of superoxide anions in specific organelles, have a protection effect on cells and can be used for effectively treating acute kidney injury.

The invention discloses a double-targeting near-infrared ratiometric fluorescent probe and application thereof in preparation of a carboxylesterase detection reagent. The structure of the double-targeting near-infrared ratiometric fluorescent probe is shown as follows: # imgabs0 #. A quinoline salt part in the fluorescent probe ZZD can target mitochondria, and the ZD structure after response can be positioned on lipid droplets. The fluorescent probe ZZD has the advantages of good response capability, excellent double-organelle targeting capability and near-infrared emission in living cells. The fluorescent probe ZZD is expected to become a useful fluorescent tool for researching lipid droplet and mitochondrial related diseases in the future.

The present invention concerns systems, materials, and methods of cell, cell organelle and / or particle separation and / or concentration utilizing magnetic levitation to affect separation. In some embodiments, the separation and / or concentration is performed in the wells of a multi-well plate.

The invention belongs to the technical field of fluorescence imaging, and particularly relates to a compound with a lysosome and mitochondria double-color imaging function and a preparation method and application thereof. The compound provided by the invention has different excitation wavelengths and fluorescence with different colors in different acid-base environments, and can be used for preparing fluorescent probes for simultaneous imaging of different organelles. The fluorescent probe provided by the invention can realize double-color imaging of living cells or lysosome and mitochondria in vivo at the same time, green fluorescence is displayed in the lysosome, red fluorescence is displayed in the mitochondria, and the lysosome and the mitochondria can be monitored in real time in a living biological sample. Meanwhile, the fluorescent probe can avoid the problems of mutual interference, staining condition exploration and the like when a commercial probe is used for researching lysosome and mitochondria. In addition, the fluorescent probe is simple in preparation process, low in cost, stable in structure and convenient to store, and has wide application space in biological function research of lysosome and mitochondria.

The invention discloses a saccharomyces cerevisiae genetic engineering strain with high squalene yield and application thereof. According to the invention, an engineering strain SquM13 is taken as a starting strain, a plurality of modification strategies associated with organelles are tried, a plurality of saccharomyces cerevisiae genetic engineering strains SY04, SY12, SY19, SY39, SY49 and SY51 are screened from the engineering strain SquM13, the result of the strain SY51 is most prominent, and the yield of squalene fermented by a 5-liter bioreactor is up to 54.62 g / L. According to the saccharomyces cerevisiae genetic engineering strain with high squalene yield and the application of the saccharomyces cerevisiae genetic engineering strain, the yield of squalene is obviously improved, and large-scale squalene industrialization becomes possible.

The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.

The invention discloses a preparation method and application of a mitochondrial targeting polypeptide modified carbon quantum dot probe, and relates to the technical field of nano biological materials and fluorescence imaging.The preparation method comprises the steps that citric acid and urea are dissolved in a dimethyl sulfoxide solution, mixed, heated, filtered, added with ethyl alcohol, centrifuged and freeze-dried, and carbon quantum dots are obtained; adding an MES buffer solution and an activating agent, reacting the polypeptide at room temperature, heating, reacting, centrifuging, dialyzing and freeze-drying to obtain polypeptide modified carbon quantum dots; the carbon quantum dots are synthesized from bottom to top, and the preparation method is simple. After polypeptide modification, the product has an obvious mitochondrial targeting effect, and the product is good in biocompatibility, so that the product can be used for imaging and dynamic tracking of living organelles. The probe developed by the invention shows obvious application potential in key cell events such as mitochondrial dynamic monitoring and the like.

The invention discloses a terahertz near-field system sample nanometer repeated positioning method, and belongs to the technical field of terahertz near-field imaging. According to the method, repeated positioning with nanoscale precision is realized through two key technical means: displacement compensation: after first scanning of a sample area is completed and reference coordinates are recorded, second scanning is carried out, and the offset of the center coordinates of a probe relative to the reference coordinates in the X-axis and / or Y-axis direction is monitored; before follow-up scanning, the reverse value of the offset is used as the compensation amount to be input into a control system so as to correct the reference coordinate; and voltage control: monitoring and controlling the voltage of the system in real time when the probe withdrawing operation is intermittently executed in the current scanning and the next scanning, and stopping probe withdrawing within one second when the voltage value rises to 180V so as to control the withdrawing distance of the probe. According to the method, the repeated positioning accuracy can be controlled within 5 nanometers, and the method is suitable for multiple times of accurate imaging of biological organelles and nano structures in semiconductors.

The invention discloses a high-activity BODIPY type photosensitizer targeting double organelles as well as a preparation method and application of the high-activity BODIPY type photosensitizer, and relates to the technical field of medical photosensitizers. The high-activity BODIPY type photosensitizer is prepared by the following steps: reacting 2, 4-dimethyl pyrrole with 4-bromobutyryl chloride to obtain a compound Br-BY containing carboxyl, then reacting with morpholine to obtain a morpholine substituted compound Mor-BY, and finally reacting with N-iodosuccinimide (NIS) to obtain a single atom I substituted BODIPY compound Mor-IBY, namely the target photosensitizer. The photosensitizer prepared by the invention can target mitochondria and lysosome in cancer cells at the same time, and can be excited by visible light; the compound has good water solubility, can generate a large amount of reactive oxygen species (ROS) under irradiation of green light (520 nm), shows excellent photodynamic activity to tumor cells, and has low toxicity to normal cells.

An imaging system uses a multi-view reflector (MVR) to simultaneously measure the three-dimensional (3D) position and orientation of a light emitter. The MVR is positioned at a back focal plane (BFP) of a light collecting optical system, and uses pyramid reflectors and / or conical reflectors to reflect light in at least two regions of the BFP to at least two detection channels of a detector. The at least two detection channels produce images that are used to determine the 3D position and orientation of a light emitter. The system may be used, for example, to image the structure of molecules or organelles in a cell, track the movement of molecules in a cell, or to study the interaction of molecules within a cell.

The invention discloses engineering mitochondria enriched with alpha2delta2 protein, the expression level of the alpha2delta2 protein in the mitochondria is higher than that of natural mitochondria, the expression quantity of the alpha2delta2 protein is 15-40 times that of the natural mitochondria, and the alpha2delta2 protein is enriched in the outer membrane of the mitochondria. The invention also discloses a preparation method of the engineered mitochondria enriched with the alpha2delta2 protein. The invention also discloses an application of the engineered mitochondria enriched with the alpha2delta2 protein in preparation of medicines for preventing and / or treating fibrosis diseases. In-vitro experiments prove that the engineered mitochondria can significantly enhance the ability of inhibiting fibroblast activation and collagen secretion, and the effect of the engineered mitochondria is far better than that of common mitochondria. The invention provides a brand-new and effective organelle level treatment scheme for solving the clinical problem of fibrosis.

The present disclosure discloses an engineered liposome with cell membrane proteins to reduce melanosome transport and a preparation method thereof, and belongs to the technical field of cosmetics and biomedicine. The present disclosure provides the engineered liposome with cell membrane proteins to reduce melanosome transport and the preparation method thereof, which is easy to operate, requires no large-scale equipment, has few additives, and a preparation process is simple and environmentally friendly. The biomimetic liposome can significantly inhibit melanin transport. The fluorescence intensity of melanosomes in keratinocytes is found to decrease by 3.5-fold in a co-culture test of melanocytes and the keratinocytes, indicating that this biomimetic liposome is very effective in inhibiting accumulation of melanin in skin keratinocytes. These findings provide an effective strategy for reducing melanosome transfer to treat hyperpigmentation, while also introducing an alternate approach for regulating cellular communication of extracellular vesicles and organelles.

The invention discloses an Apopsin3 mutant and a method for regulating an environment in a plant cell to be acidic, and belongs to the technical field of pH regulation in the plant cell. The regulation and control method comprises the following steps: mutating 83rd and 120th amino acids of the light-operated hydrogen ion pump protein Apopsin3, converting into hydrogen ion channels, expressing the hydrogen ion channels on a cell membrane in a targeting manner, and stimulating with green light of 40-500 [mu] W / mm < 2 > and 520-560 nm so as to regulate the pH in the cell to be acidic. By adopting the cell specific promoter and performing site-specific mutagenesis modification on the specific hydrogen ion pump, accurate pH regulation and control on specific cells or organelles can be realized. An Apopsin3 (FARH) gene is fused with a cell membrane targeting sequence, and under the stimulation of specific light, only the pH of cytoplasm is obviously changed, and the pH of other cell regions is not influenced.

This invention provides the nucleic acid molecules encoding novel small monomeric near-infrared fluorescent protein miRFP670nano, variants and derivatives thereof as well as proteins and peptides encoded by these nucleic acids. The invention also relates to derivatives, homologues, or mutants of the specific proteins referenced above as well as fragments of the nucleic acids and the peptides encoded thereby. The invention further relates to host-cells, stable cell lines and transgenic organisms comprising above-referenced nucleic acid molecules. The present invention also refers to methods of making and using small monomeric near-infrared fluorescent proteins derived from cyanobacteriochromes. The presented protein and its derivatives find use in a variety of applications and approaches, including labeling of biomolecules, cells or cell organelles, detecting protein-protein interactions, and generation of genetically encoded fluorescent biosensor.