22 results about "Rare cell" patented technology

This invention discloses a membrane-based enrichment slide preparation device for rare cell samples, relating to the field of cell sample processing technology. It includes a base and a support mounted on top of the base, and further comprises: a housing with a placement stage mounted on the top; a positioning mechanism including multiple positioning cylinders rotatably mounted inside the placement stage, sample vials slidably mounted on the positioning cylinders, and filter cylinders disposed inside the sample vials; a centrifugation mechanism for driving the multiple positioning cylinders to rotate; multiple glass slides; a lifting and transfer mechanism mounted inside the housing for driving the multiple filter cylinders to rise and fall; and an adjustment mechanism for fixing and adjusting the displacement of the glass slides. By concentrating the filtered target cells on top of the membrane, and after driving the membrane to move to the bottom of the glass slide, the target cells concentrated on top of the membrane can be evenly transferred to the glass slide, improving the enrichment slide preparation effect of rare cell samples and avoiding the problem of poor slide preparation due to target cell dispersion.

The application provides a single-cell transcriptome cell type automatic annotation method and device based on consensus voting, electronic equipment and storage medium, relates to the field of medical biotechnology, and integrates a plurality of initial annotation results obtained by a plurality of cell type annotation methods by applying an ensemble learning strategy, so as to reduce errors that may exist in a single annotation method, and improve the accuracy and robustness of cell type annotation. In addition, the method uses single-cell transcriptome data of a sample, combines rich prior knowledge and strong reasoning ability of a large language model, and has the ability to discover rare cell types, so as to effectively identify rare cell types, widen the application range of cell type annotation, and improve the general tissue annotation capability of cell types.

To provide methods that provide a highly sensitive method for detecting rare cell types in a cell population and can overcome limited sensitivity in conventional methods of detecting stem cells caused by background expression of stem cell genes in non-stem cells and by cell loss during sample preparation.SOLUTION: A method of detecting the presence of or confirming the absence of target cells in a cell population comprises: specific steps (a) to (e).SELECTED DRAWING: None

The invention relates to the technical field of cell recognition, and discloses a rare cell population recognition method, device and equipment and a storage medium, and the method comprises the steps: obtaining an original gene expression matrix, preprocessing the original gene expression matrix to obtain a target gene expression matrix, and constructing a cell leaf node graph and a K neighbor graph; integrating the cell leaf node graph and the K neighbor graph to obtain an enriched cell leaf node graph; performing node embedding on the enriched cell leaf node graph to map all target cells in the target gene expression matrix to a target dimension embedding space to obtain a cell embedding vector set; and performing clustering analysis and neighbor error analysis on all target cells in the cell embedding vector set, and identifying a rare cell population. According to the scheme, the limitation of single-dimension information is overcome, the complex relationship between cells can be more comprehensively and deeply described from two complementary perspectives of a functional mechanism and a phenotype state, and the recognition accuracy of a rare cell population is improved.

The present disclosure provides a rare cell capture reagent combination and a capture method thereof, which comprises a substrate, a capture antibody, a blocking solution, an incubation buffer and magnetic beads; the substrate is a compound of formula (I) and its derivatives; the capture antibody includes but is not limited to one or more of anti-CK, anti-EpCAM, anti-PD-L1, anti-HER2, anti-TROP2, anti-c-MET, anti-Claudin18.2, anti-EGFR, anti-MUC1, anti-HER3, anti-CD31, anti-CD44 antibody and the like; the capture antibody is an oxidoreductase-labeled antibody; and the magnetic beads are avidin-labeled magnetic beads. The reagent combination and capture method can be widely used for capturing and separating rare cells with low expression of specific antigens. Compared with the traditional secondary antibody capture method, the capture efficiency of the present disclosure is higher, and has higher capture sensitivity while not affecting the specificity.

1. The name of the design product: full-automatic non-destructive separation and enrichment of rare cell instrument. 2. The use of the design product: efficiently and accurately separate and enrich rare cell types from complex biological samples. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The application discloses an automatic scanning and picking device and method for single-cell operation of rare cells, and comprises the following: an automatic multi-color fluorescence scanning and recognizing module, the automatic multi-color fluorescence scanning and recognizing module comprising a multi-channel fluorescence microscope, a planar sliding table being installed on the multi-channel fluorescence microscope, and the multi-channel fluorescence microscope being connected with an image acquisition and analysis system; a multi-angle console, the multi-angle console comprising a base, the base being installed on the planar sliding table, a rotating assembly, a first pitching assembly and a second pitching assembly being installed on the base, a micro-operation platform, the micro-operation platform comprising a Z-axis sliding table installed on the multi-channel fluorescence microscope, and a capillary glass microneedle being fixed at the tail end of an execution end of the Z-axis sliding table; a nanometer microcavity chip, the nanometer microcavity chip being placed on an object table; and an upper computer, the upper computer being used for controlling the operation of the whole device. The application greatly improves the position stability in the process of processing of rare cells, and is suitable for the characteristics of low quantity and difficult operation of the rare cells.

This invention discloses a method for microscopically separating and rescuing cells from heavily contaminated cell culture media, belonging to the field of biotechnology. This invention combines flow cytometry sorting technology with penicillin-streptomycin for the separation, rescue, and culture of healthy cells. The method provided by this invention can effectively rescue heavily contaminated cells, remove their contamination state, and make them usable again. This will greatly facilitate cell preservation, passage, and culture, effectively avoid the waste of cell resources, and improve the preservation rate of rare cell resources, providing strong support for the smooth progress of scientific research.

The application discloses a capturing device suitable for capturing rare cells and a preparation method thereof. The capturing device suitable for capturing rare cells comprises a substrate with at least a metal surface layer, a coating layer arranged on the surface of the substrate and a capturing agent connected to the coating layer, the capturing agent can specifically combine with target rare cells, and the coating layer comprises mercapto polyethylene glycol carboxyl and a mercapto compound. The application can reduce non-specific adsorption of background cells and proteins, and improve the purity of captured target cells.

The utility model relates to the technical field of cell sorting equipment, and discloses an auxiliary device for fixing a small-capacity collecting tube of a flow sorter, which comprises an intubation tube, a collecting tube placing cavity is arranged in the intubation tube, the intubation tube comprises a tube body and a tube edge, the tube edge is positioned on the periphery of the top of an opening side of the tube body and extends outwards, and the outer diameter of the tube edge is larger than the inner diameter of a groove of an adapter. The collection tube placing cavity in the intubation tube is used for placing small-capacity collection tubes, such as 0.2 mL, 0.3 mL and 0.5 mL collection tubes, and the intubation tube can be placed in the groove of the adapter, so that stable placement of the small-capacity collection tubes is met, collection of rare cells is realized, cell loss is reduced, and the yield is improved.

The invention generally relates to mass tag analysis for rare cells and cell free molecules. In certain embodiments, the invention provides an apparatus including an essentially non-absorbent membrane having at least one pore, a microwell operably associated with the essentially non-absorbent membrane, and an electric field generator. The apparatus may be configured such that an electric field produced by the electric field generator operably interacts with a sample in the microwell and expels a droplet of the sample through the at least one pore in the essentially non-absorbent membrane. In certain embodiments, apparatuses of the invention are used for detection, and optionally quantification, of a target analyte from a heterogeneous sample, such as a rare target analyte (e.g., rare cell) from a biological sample.

The invention provides a rare type cell detection method and system, and belongs to the technical field of data processing, and the method comprises the following steps: inputting a single cell gene expression or apparent characteristic matrix including N cells and M characteristics; calculating a neighborhood internal connectivity Q value of each cell according to the number of connecting edges between each cell and k neighbor cells (including itself) and the total number of edges; initializing each cell into an independent class group, performing network diffusion of class group labels based on a gradient of a neighborhood internal connectivity Q value, and determining a cell class group; and merging and optimizing a part of low-quality class groups through the internal connectivity Qc value of the cell class groups so as to determine a final cell clustering result. According to the technology, the rare cell population can be quickly found from single-omics, multi-omics and high-resolution spatial omics data, and the prediction efficiency, accuracy and applicability are improved.

The invention provides a rare cell detection method, device and system, and relates to the technical field of rare cell detection. The method comprises the following steps: acquiring a multi-frequency impedance signal of a to-be-detected cell sample; extracting a plurality of electrical phenotypic features based on the multi-frequency impedance signal; inputting the various electrical phenotypic characteristics of the cell sample to be detected into a pre-trained cell detection model, and outputting a rare cell detection result; wherein the multi-frequency impedance signal is obtained by the following steps of: injecting a prepared cell sample into the microfluidic device, realizing single cell arrangement of the cell sample to be detected through hydrodynamic focusing, and synchronously measuring the impedance signal of the single cell at different frequencies to obtain the multi-frequency impedance signal. The electrical phenotypic features are extracted from the multi-frequency impedance signals, the extracted electrical phenotypic features can reflect subtle differences of the cells under various electrical frequencies, the discrimination degree of a classification model is enhanced, accurate detection of the rare cells is achieved, the rare cells and other cells are accurately distinguished, and the classification accuracy is improved.

This invention relates to a surface coating for capture circulating rare cells, comprising a nonfouling composition to prevent the binding of non-specific cells and adsorption of serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the nonfouling and bioactive compositions. The invention also provide a surface coating for capture and purification of a biological substance, comprising a releasable composition to release the non-specific cells and other serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the releasable and bioactive compositions. The present invention also discloses a novel microfluidic chip, with specific patterned microstructures to create a flow disturbance and increase the capture rate of the biological substance.

1. The name of the design product: rare cell detection system (full-automatic high-throughput). 2. The use of the design product: for full-automatic sample processing and flow analysis of circulating tumor-related rare cells in blood samples in the field of early screening and early diagnosis of tumors. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view 1.

The present application relates to the technical field of single cell RNA sequencing, and discloses a CellRanger single cell RNA sequencing data analysis method, which comprises hierarchical processing of cell quality screening and a tolerant mode of gene expression screening. The method provided by the present application not only strictly guarantees the integrity of various functional indicators in the cells finally stored in the library, but also retains part of the cell information which may have biological significance due to the loss of candidate markers caused by low overall transcriptional activity through further judgment of the low expression state of the cells to be determined. Through this method, the reliability of subsequent analysis data can be ensured, and the information of low expression or rare cells missed due to strict one-size-fits-all standards can be reduced as much as possible.

An embodiment in accordance with the present invention provides two devices for the isolation of rare cell populations such as circulating tumor cells (CTCs). Both devices use magnetic fields to manipulate cells that are bounded to paramagnetic particles (PMP). One device uses surface tension and a sieve for cell filtration, whereas the other allows for the direct transfer of cells onto a standard microscope slide. Subsequent processing steps may be directly performed on the devices thereby minimizing cell losses. The first device is referred to as the ST (surface tension) device and the second device is referred to as the DT (direct transfer) device. The ST device can also be considered as a chip for the isolation of the rare cell populations.

The invention discloses a method and a kit for detecting RNA (Ribonucleic Acid) methylation modification by using a small amount of cells, which adopt the technologies of antibody crosslinking, reverse transcription, in-vitro transcription, sequencing analysis and the like, so that the method and the kit provided by the invention have the advantages that m6Am modification sites can be effectively detected; the resolution of an m6A modification site is improved; and m6A and m6Am modifications in trace rare cells can be effectively detected.

The present disclosure provides technologies for efficient, sensitive, and / or accurate separation and / or isolation of subpopulations of cells, including rare cells, and methods of detecting of the presence or absence of genetic variants in isolated subpopulations of cells.

Described are systems for cell culture analysis and methods for the use thereof, wherein the system comprises a plurality of cell culture chambers and a receptacle for receiving the cell culture chambers, v herein the receptacle comprises one or more receptacle chambers each having a. wail and a. floor. Each cell culture chamber comprises a side wall, an opening on the top of the cell culture chamber, and a semi-permeable membrane on the bottom of the cell culture chamber, and i) wherein the membrane is a small distance from the floor of the receptacle chamber and / or ii) wherein the total surface area of the membrane on the plate is 50% or more of the surface area of the receptacle bottom. The systems and methods allow the detection of rare cell types, rare events, and / or higher cell numbers, including the ability to contact the cells with different analytes for extended times and / or for multiple treatment cycles.

The application provides a single-cell rare cell type identification method and system based on a divide-and-conquer strategy, comprising the following steps: S1, using a variational autoencoder to encode single-cell transcriptome data into Gaussian distribution hidden variables; S2, performing spectral clustering on the Gaussian distribution hidden variables to obtain a single-cell coarse-grained clustering result; and S3, optimizing the single-cell coarse-grained clustering result based on intra-class cluster loss and inter-class cluster loss until the intra-class cluster loss and the inter-class cluster loss are minimized to obtain a single-cell clustering result containing rare cell types. The application can overcome the deficiency that previous single-cell clustering and identification methods do not optimize rare cell types, leading to misclassification or difficulty in identifying rare cell types.

The invention provides a multi-omics rare cell annotation method and system, computer equipment and a medium, and belongs to the technical field of biological information and computers. The method comprises the following steps: constructing a heterograph containing cell, gene and chromatin accessibility peak value nodes by integrating single cell RNA sequencing and ATAC sequencing data; a pre-fusion and graph fusion two-stage strategy is adopted, deep extraction and cross-modal fusion are carried out on the multi-omics features, and high-order node embedding is obtained; and further constructing reconstruction, clustering alignment and intra-class similarity multi-objective loss functions, and realizing accurate identification and annotation of rare cell types through joint optimization. By adopting the multi-omics rare cell annotation method and system, the computer equipment and the medium, the limitation of single-mode information is overcome, the capturing capability of low-abundance cell subgroups is improved, and the method has good generalization and biological interpretability and can be applied to the fields of tumor microenvironment analysis, immune response mechanism research, developmental biology and the like.