44 results about "Rare cell" patented technology

The invention discloses a method for separating, sorting and concentrating rare cells. The method comprises the following steps: pretreating a to-be-separated sample, adding the pretreated sample into a sample accommodating assembly in the cell separation, sorting and concentration device, and controlling the sample accommodating assembly to separate, sort and enrich the to-be-separated sample through a filtering and sorting mechanism to obtain rare cells. The specific cell separating, sorting and concentrating device is designed, and the method for separating, sorting and concentrating the rare cells is developed based on the device, so that the recovery rate and concentration rate of the rare cells are improved, the operation is simplified, the smear operation process is further developed, the separation, sorting, enrichment and smear processes of the cells can be realized, and the production efficiency is improved. The cell loss is prevented, the smear quality is improved, and the smear time is shortened.

The invention discloses a method for high-purity sorting and capturing of rare cells. According to the method for high-purity sorting and capturing of the rare cells, the cells are spread firstly, then the positions of the target cells are searched and determined, then a probe is used for discharging a buffer solution outwards to blow away non-target cells around the target cells, and then fluid power is used for capturing the target cells without damage. Finally, the target cells can be released into a receiving container, and high-purity sorting and capturing of the rare cells are achieved. A device matched with the method is simple in structure and easy to operate, and the method has the advantages that repeated and massive dilution is not needed, the cell sample spreading speed is high, the target cell sorting purity is high, the omission rate is low, in-situ capture is achieved, and repeatability is good. The method is suitable for the fields of single cell screening and analysis, single cell culture, single cell drug screening, single molecule analysis, gene screening, sample purification, antibody screening, microbiological research and the like.

Provided is a method and apparatus for cell staining without cell loss, and more particularly, to a method and apparatus for cell staining without cell loss during treatment of a staining reagent or washing reagent by immobilizing cells to be analyzed in a phase change material before staining. A method for cell staining without cell loss may effectively prevent cell loss during staining and analysis of rare cells that are becoming important in clinical diagnosis, and thus may facilitate observation, analysis and diagnosis thereby even with a trace amount of sample. In addition, as the method for cell staining without cell loss allows immobilization of cells, it makes discrimination and isolation of single cells easy, and may be effectively used in analysis of the isolated cells.

This disclosure provides genomics-based methods that can be used to identify, quantify, and characterize rare cell types, including circulating tumor cells.

This invention discloses a membrane-based enrichment slide preparation device for rare cell samples, relating to the field of cell sample processing technology. It includes a base and a support mounted on top of the base, and further comprises: a housing with a placement stage mounted on the top; a positioning mechanism including multiple positioning cylinders rotatably mounted inside the placement stage, sample vials slidably mounted on the positioning cylinders, and filter cylinders disposed inside the sample vials; a centrifugation mechanism for driving the multiple positioning cylinders to rotate; multiple glass slides; a lifting and transfer mechanism mounted inside the housing for driving the multiple filter cylinders to rise and fall; and an adjustment mechanism for fixing and adjusting the displacement of the glass slides. By concentrating the filtered target cells on top of the membrane, and after driving the membrane to move to the bottom of the glass slide, the target cells concentrated on top of the membrane can be evenly transferred to the glass slide, improving the enrichment slide preparation effect of rare cell samples and avoiding the problem of poor slide preparation due to target cell dispersion.

The application provides a single-cell transcriptome cell type automatic annotation method and device based on consensus voting, electronic equipment and storage medium, relates to the field of medical biotechnology, and integrates a plurality of initial annotation results obtained by a plurality of cell type annotation methods by applying an ensemble learning strategy, so as to reduce errors that may exist in a single annotation method, and improve the accuracy and robustness of cell type annotation. In addition, the method uses single-cell transcriptome data of a sample, combines rich prior knowledge and strong reasoning ability of a large language model, and has the ability to discover rare cell types, so as to effectively identify rare cell types, widen the application range of cell type annotation, and improve the general tissue annotation capability of cell types.

To provide methods that provide a highly sensitive method for detecting rare cell types in a cell population and can overcome limited sensitivity in conventional methods of detecting stem cells caused by background expression of stem cell genes in non-stem cells and by cell loss during sample preparation.SOLUTION: A method of detecting the presence of or confirming the absence of target cells in a cell population comprises: specific steps (a) to (e).SELECTED DRAWING: None

The invention relates to the technical field of cell recognition, and discloses a rare cell population recognition method, device and equipment and a storage medium, and the method comprises the steps: obtaining an original gene expression matrix, preprocessing the original gene expression matrix to obtain a target gene expression matrix, and constructing a cell leaf node graph and a K neighbor graph; integrating the cell leaf node graph and the K neighbor graph to obtain an enriched cell leaf node graph; performing node embedding on the enriched cell leaf node graph to map all target cells in the target gene expression matrix to a target dimension embedding space to obtain a cell embedding vector set; and performing clustering analysis and neighbor error analysis on all target cells in the cell embedding vector set, and identifying a rare cell population. According to the scheme, the limitation of single-dimension information is overcome, the complex relationship between cells can be more comprehensively and deeply described from two complementary perspectives of a functional mechanism and a phenotype state, and the recognition accuracy of a rare cell population is improved.

The present invention discloses a method for high-purity sorting and capturing of rare cells. The method for high-purity sorting and capturing of rare cells in the present invention first spreads the cells, then locates and determines the position of the target cells, then uses a probe to expel the buffer outward to disperse the non-target cells around the target cells, and then uses hydrodynamic force to capture the target cells without damage. Finally, the target cells can be released into a receiving container to achieve high-purity sorting and capturing of rare cells. The device used in the method of the present invention has a simple structure and is easy to operate. The method has the advantages of not requiring repeated large-scale dilution, fast spreading speed of cell samples, high sorting purity of target cells, low omission rate, in-situ capture, and good repeatability, and is applicable to fields such as single-cell screening and analysis, single-cell culture, single-cell drug screening, single-molecule analysis, gene screening, sample purification, antibody screening, and microbial research.

The present disclosure provides a rare cell capture reagent combination and a capture method thereof, which comprises a substrate, a capture antibody, a blocking solution, an incubation buffer and magnetic beads; the substrate is a compound of formula (I) and its derivatives; the capture antibody includes but is not limited to one or more of anti-CK, anti-EpCAM, anti-PD-L1, anti-HER2, anti-TROP2, anti-c-MET, anti-Claudin18.2, anti-EGFR, anti-MUC1, anti-HER3, anti-CD31, anti-CD44 antibody and the like; the capture antibody is an oxidoreductase-labeled antibody; and the magnetic beads are avidin-labeled magnetic beads. The reagent combination and capture method can be widely used for capturing and separating rare cells with low expression of specific antigens. Compared with the traditional secondary antibody capture method, the capture efficiency of the present disclosure is higher, and has higher capture sensitivity while not affecting the specificity.

1. The name of the design product: full-automatic non-destructive separation and enrichment of rare cell instrument. 2. The use of the design product: efficiently and accurately separate and enrich rare cell types from complex biological samples. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

Methods are provided for detection of a target cell type within a cell population, and compositions are provided comprising cells and an indicator that indicates the number of cells of the target cell type in the cell population. Examples are provided in which these methods are used to detect human embryonic stem cells within a differentiated cell population with exquisite sensitivity. Differentiated cells produced from embryonic stem cells can be characterized by these methods before transplantation into a recipient, thereby providing further assurance of safety.

The application discloses an automatic scanning and picking device and method for single-cell operation of rare cells, and comprises the following: an automatic multi-color fluorescence scanning and recognizing module, the automatic multi-color fluorescence scanning and recognizing module comprising a multi-channel fluorescence microscope, a planar sliding table being installed on the multi-channel fluorescence microscope, and the multi-channel fluorescence microscope being connected with an image acquisition and analysis system; a multi-angle console, the multi-angle console comprising a base, the base being installed on the planar sliding table, a rotating assembly, a first pitching assembly and a second pitching assembly being installed on the base, a micro-operation platform, the micro-operation platform comprising a Z-axis sliding table installed on the multi-channel fluorescence microscope, and a capillary glass microneedle being fixed at the tail end of an execution end of the Z-axis sliding table; a nanometer microcavity chip, the nanometer microcavity chip being placed on an object table; and an upper computer, the upper computer being used for controlling the operation of the whole device. The application greatly improves the position stability in the process of processing of rare cells, and is suitable for the characteristics of low quantity and difficult operation of the rare cells.

This invention discloses a method for microscopically separating and rescuing cells from heavily contaminated cell culture media, belonging to the field of biotechnology. This invention combines flow cytometry sorting technology with penicillin-streptomycin for the separation, rescue, and culture of healthy cells. The method provided by this invention can effectively rescue heavily contaminated cells, remove their contamination state, and make them usable again. This will greatly facilitate cell preservation, passage, and culture, effectively avoid the waste of cell resources, and improve the preservation rate of rare cell resources, providing strong support for the smooth progress of scientific research.

The application discloses a capturing device suitable for capturing rare cells and a preparation method thereof. The capturing device suitable for capturing rare cells comprises a substrate with at least a metal surface layer, a coating layer arranged on the surface of the substrate and a capturing agent connected to the coating layer, the capturing agent can specifically combine with target rare cells, and the coating layer comprises mercapto polyethylene glycol carboxyl and a mercapto compound. The application can reduce non-specific adsorption of background cells and proteins, and improve the purity of captured target cells.

The invention relates to the technical field of medical instruments, and provides a rare cell capturing instrument. The rare cell capturing instrument comprises a cell capturing instrument main body, at least one micro-fluidic chip and a fluid control unit, the cell capturing instrument main body is provided with a blood sample accommodating cavity, at least one culture medium accommodating cavity, a dyeing cavity, a rare cell collecting cavity and a waste liquid accommodating cavity; the micro-fluidic chip is arranged on the cell capturing instrument main body and is provided with a first port, a second port, a third port and a fourth port; the blood sample accommodating cavity, the culture medium accommodating cavity, the dyeing cavity, the rare cell collecting cavity and the waste liquid accommodating cavity are communicated with the first opening to the fourth opening through the fluid control unit; the fluid control unit is used for controlling liquid in the blood sample accommodating cavity, the dyeing cavity and the at least one culture medium accommodating cavity to flow to the rare cell collecting cavity and the waste liquid accommodating cavity through the micro-fluidic chip. The technical problems that in the prior art, a rare cell capturing instrument is large in operation difficulty and low in operation efficiency are solved.

The invention relates to a method for full-automatic lossless separation and enrichment of rare cells, which utilizes a micro-flow device, a pressure sensor and a full-automatic control system from bottom to top, and the device can realize efficient separation and enrichment of rare cells without pre-treating thick cell liquid. By accurately controlling fluid dynamic conditions and optimizing the cell flow velocity and pressure, the method can maintain the cell activity and integrity and improve the capture rate and purity of rare cells at the same time. The design of the device allows separated and enriched rare cells to be directly collected into a collection bottle containing a cell culture medium. By means of the design, it is ensured that cells are in a proper growth environment, follow-up cell culture, downstream analysis and treatment are facilitated, and therefore the experiment efficiency and the result reliability are improved. According to the method, the traditional process is simplified, the sample treatment time is shortened, and an efficient and reliable solution is provided for cytological research and clinical diagnosis.

Provided are a detection method and a detection device that allow highly accurate detection of exosomes or rare cells. Exosomes derived from first cells are acquired (S10), and the surfaces of the exosomes are modified (S11). Second cells of the same type as the first cells are acquired (S12), and through homotyping, exosomes having modified surfaces are selectively incorporated into the second cells together with labels (S13). A signal correlated with the number of the second cells in which the surface-modified exosomes have been incorporated is acquired from a label signal (S14), and the exosomes or the second cells are detected.

The invention discloses a single-cell chromatin open data-oriented cell type automatic annotation method. The method comprises the following steps: step 1, carrying out TF-IDF-based data preprocessing; step 2, carrying out type equalization processing on the training set; step 3, training the MLP by using the processed training set obtained in the step 2 through comparative learning to obtain a trained MLP; 4, performing multi-level annotation by utilizing low-dimensional representation: respectively obtaining the low-dimensional representation of the cells by utilizing the trained MLP, and respectively obtaining a first soft label annotation matrix, a second soft label annotation matrix and a cell type identification result according to the low-dimensional representation of each cell; and calculating a cell type specific chromatin accessible peak according to the second soft label annotation matrix, and integrating the first soft label annotation matrix, the second soft label annotation matrix and a cell type identification result to obtain a final annotation result. The annotation performance is accurate, and rare cell types can be annotated and unknown cell types can be identified.

The present invention provides a microdevice for measuring the water permeability coefficient of a single cell membrane, comprising an upper chip, a film and a lower chip stacked in sequence; the front judgment channel of the upper chip, the front judgment electrode and the film of the lower chip constitute the front judgment area, and the single cell capture channel of the upper chip, the differential electrode pair and the film of the lower chip constitute the cell membrane water permeability coefficient measurement area. This microdevice based on single cell capture and cell impedance spectroscopy can measure the water permeability coefficient of a single cell membrane more accurately, quickly and efficiently, which is conducive to reducing the loss of rare cells in the measurement.

The invention discloses a rare cell capturing device and a cell heterogeneity analysis method, the device comprises: a sample liquid inlet for receiving a sample liquid; the first conveying channel is connected with the sample liquid inlet; the filtering area is connected with the first conveying channel and is used for filtering the sample liquid to obtain target liquid; the cell zoning and separating area is connected with the filtering area and is used for capturing rare cells from the target liquid; the second conveying channel is connected with the cell partition separation region; and the filtrate outlet is connected with the second conveying channel and is used for outputting the filtrate. The cell separation is realized, and the cell purity, the recovery rate and the sample capacity are improved. The method can be widely applied to the technical field of cell separation.

The invention discloses an oocyte transmission electron microscope sample preparation method, and belongs to the technical field of electron microscope sample preparation. On the basis of conventional ultrathin section technology sample preparation, a single or a very small amount of oocytes are dyed with eosin and then are pre-coated with a human granular cell line, so that the form of the oocytes is maintained, and preparation of a high-quality oocyte transmission electron microscope sample is realized; the visualization of the oocyte and the operability of the whole sample are realized in the sample slicing process, so that blind cutting and cell loss are avoided, the preparation process of the oocyte sample is optimized, the success rate of the transmission electron microscope sample of the oocyte is improved, and the detection efficiency is improved. A solution is provided for high-quality electron microscope sample preparation of very few precious oocytes and other rare cells of human or mice, so that ultrastructure observation and pathological diagnosis become possible.

The utility model relates to the technical field of cell sorting equipment, and discloses an auxiliary device for fixing a small-capacity collecting tube of a flow sorter, which comprises an intubation tube, a collecting tube placing cavity is arranged in the intubation tube, the intubation tube comprises a tube body and a tube edge, the tube edge is positioned on the periphery of the top of an opening side of the tube body and extends outwards, and the outer diameter of the tube edge is larger than the inner diameter of a groove of an adapter. The collection tube placing cavity in the intubation tube is used for placing small-capacity collection tubes, such as 0.2 mL, 0.3 mL and 0.5 mL collection tubes, and the intubation tube can be placed in the groove of the adapter, so that stable placement of the small-capacity collection tubes is met, collection of rare cells is realized, cell loss is reduced, and the yield is improved.

The invention generally relates to mass tag analysis for rare cells and cell free molecules. In certain embodiments, the invention provides an apparatus including an essentially non-absorbent membrane having at least one pore, a microwell operably associated with the essentially non-absorbent membrane, and an electric field generator. The apparatus may be configured such that an electric field produced by the electric field generator operably interacts with a sample in the microwell and expels a droplet of the sample through the at least one pore in the essentially non-absorbent membrane. In certain embodiments, apparatuses of the invention are used for detection, and optionally quantification, of a target analyte from a heterogeneous sample, such as a rare target analyte (e.g., rare cell) from a biological sample.

The invention provides a rare type cell detection method and system, and belongs to the technical field of data processing, and the method comprises the following steps: inputting a single cell gene expression or apparent characteristic matrix including N cells and M characteristics; calculating a neighborhood internal connectivity Q value of each cell according to the number of connecting edges between each cell and k neighbor cells (including itself) and the total number of edges; initializing each cell into an independent class group, performing network diffusion of class group labels based on a gradient of a neighborhood internal connectivity Q value, and determining a cell class group; and merging and optimizing a part of low-quality class groups through the internal connectivity Qc value of the cell class groups so as to determine a final cell clustering result. According to the technology, the rare cell population can be quickly found from single-omics, multi-omics and high-resolution spatial omics data, and the prediction efficiency, accuracy and applicability are improved.

Disclosed herein are compositions and methods of fixing and staining rare cells. Further, disclosed herein are methods of identifying circulating tumor cells (CTC). In some embodiments, the method includes: imaging a cell sample to identify a cell of interest; determining a first pixel intensity of a stained nuclear area; determining a second pixel intensity of a background area; calculating a ploidy status of the cell of interest by subtracting the second pixel intensity from the first pixel intensity; and determining whether the cell of interest is a CTC based on the ploidy status. The method may be computer implemented, such that the method uses a machine learning algorithm to identify a feature; process the feature to extract a parameter of interest; analyze the parameter of interest; and when the parameter of interest is greater than or less than a pre-determined threshold, classify the cell of interest as a CTC.

Provided are methods and apparatus for a novel serial enrichment and dilution (SED) process to sort target cells, including rare cells, from a mixture, allowing for the isolation of the target cells using serial dilution. The apparatus including a flow cell with a single flow path having an analysis zone between an inlet channel and a first outlet channel, a valve within the first outlet channel, and a second outlet channel attached to the first outlet channel, the valve opening one of the first or second outlet channel and closing the other of the first or second outlet channel when target cells are detected.

The invention belongs to the technical field of molecular biology, and particularly relates to a method, electronic equipment and a system for detecting rare cell mutation based on single cell sequencing. The method comprises the following steps: acquiring first single cell sequencing data of a to-be-detected sample and second single cell sequencing data of a target region in the to-be-detected sample; acquiring a map of rare cells in the to-be-detected sample according to the first single cell sequencing data; acquiring mutation site information in the to-be-detected sample according to the second single cell sequencing data; and screening the mutation site information of the rare cells from the mutation site information of the to-be-detected sample according to the spectrum of the rare cells. The method supports cell bar code analysis; mutation detection is performed based on a single cell level, and rare mutation is reserved to the maximum extent; and a prior mutation site is not needed as a reference.

The invention provides a rare cell detection method, device and system, and relates to the technical field of rare cell detection. The method comprises the following steps: acquiring a multi-frequency impedance signal of a to-be-detected cell sample; extracting a plurality of electrical phenotypic features based on the multi-frequency impedance signal; inputting the various electrical phenotypic characteristics of the cell sample to be detected into a pre-trained cell detection model, and outputting a rare cell detection result; wherein the multi-frequency impedance signal is obtained by the following steps of: injecting a prepared cell sample into the microfluidic device, realizing single cell arrangement of the cell sample to be detected through hydrodynamic focusing, and synchronously measuring the impedance signal of the single cell at different frequencies to obtain the multi-frequency impedance signal. The electrical phenotypic features are extracted from the multi-frequency impedance signals, the extracted electrical phenotypic features can reflect subtle differences of the cells under various electrical frequencies, the discrimination degree of a classification model is enhanced, accurate detection of the rare cells is achieved, the rare cells and other cells are accurately distinguished, and the classification accuracy is improved.