Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations

a nucleic acid and individual cell technology, applied in the field of nucleic acid chemistry and biochemical assays, can solve the problems of insufficient progress in the validation of the clinical utility of ctc detection as a prognostic indicator, low concentration of ctc in peripheral blood or other body fluids, etc., and achieve the effect of improving the specificity of the assay
US20080038725A1Inactive Publication Date: 2008-02-14ADVANCED CELL DIAGNOSTICS INC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
ADVANCED CELL DIAGNOSTICS INC
Publication Date
2008-02-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a non-provisional utility patent application claiming priority to and benefit of the following prior provisional patent application: U.S. Ser. No. 60 / 691,834, filed Jun. 20, 2005, entitled “Method of Detecting and Enumerating Rare Cells from Large Heterogeneous Cell Populations” by Luo and Chen, which is incorporated herein by reference in its entirety for all purposes.FIELD OF THE INVENTION

[0002] The invention relates generally to nucleic acid chemistry and biochemical assays. More particularly, the invention relates to methods for in situ detection of nucleic acid analytes in single cells. The invention also relates to detection and identification of single cells, particularly rare cells.BACKGROUND OF THE INVENTION

[0003] Ample evidence has demonstrated that cancer cells can dissociate from the primary tumor and circulate in the lymph node, bone marrow, peripheral blood or other body fluids. These circulating tumor cell...

Claims

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