47 results about "Direct binding" patented technology

A method for identifying compounds binding to HCV polymerase comprising the steps of: contacting said HCV polymerase or an analog thereof with a probe formula I:

wherein A is O, S, N, NR¹, or CR¹, wherein R¹ is defined herein;

• ----- represents either a single or a double bond;
• R² is selected from: H, halogen, R²¹, OR²¹, SR²¹, COOR²¹, SO₂N(R²²)₂, N(R²²)₂, CON(R²²)₂, NR²²C(O)R²² or NR²²C(O)NR²² wherein R²¹ and each R²² is defined herein;
• B is NR³ or CR³, wherein R³ is defined herein;
• with the proviso that, when A is not N, then one of A or B is either CR¹ or CR³,
• K is N or CR⁴, wherein R⁴ is defined herein;
• L is N or CR⁵, wherein R⁵ has the same definition as R⁴ defined above;
• M is N or CR⁷, wherein R⁷ has the same definition as R⁴ defined above;
• R⁵ is C(Y¹)Z wherein Y¹ is O or S; and
• Z is N(R^6a)R⁶ or OR⁶, wherein R^6a is H or alkyl or NR⁶¹R⁶² wherein R⁶¹ and R⁶² are defined herein; and R⁶ is H, alkyl, cycloalkyl, alkenyl, Het, alkyl-aryl, alkyl-Het; or R⁶ is

wherein R⁷ and R⁸ and Q are as defined herein;

• Y² is O or S;
• R⁹ is H, (C_1-6 alkyl), (C_3-7)cycloalkyl or (C_1-6)alkyl-(C_3-7)cycloalkyl, aryl, Het, (C_1-6)alkyl-aryl or (C_1-6)alkyl-Het, all of which optionally substituted with R⁹⁰; or R⁹ is covalently bonded to either of R⁷ or R⁸ to form a 5- or 6-membered heterocycle; or a salt thereof; where the probe comprises a detectable label attached to any suitable position, whereby said probe binds to an HCV polymerase or an analog thereof and is capable of being displaced by an inhibitor thereof.

The invention provides methods for the detection, quantification, identification and structural analysis of one or more molecules. Mass spectrometry (MS) is not a universal detector as all molecules do not ionize equally well leading to poor signal to quantity information. MS can be optimized to identify the specific mass of a binding component when the presence of a material is known. Colorimetric resonant reflectance optical sensors provide a universal mass detector in that nearly all biological masses give equally proportional signals. The combined methods allow selection and or detection with quantification of all masses binding to the sensor with the ability to identify specific molecules by their individual masses and structure analyses.

The invention relates to a porous concrete ecological capsule slop protection process, which comprises making ecological capsules which are braided by fiber material such as palm ropes and twines and the like, storing doras, water-retention material, slow-release fertilizer, insecticide and plant seeds, placing the ecological capsules in the grooves of the porous concrete ecological capsule blocks, matching an upper and a lower porous concrete ecological capsule blocks through the way of bolt or direct binding, placing the porous concrete ecological capsule blocks on hard slope protection, connecting each porous concrete ecological capsule block with bolts or jump rings, and binding the space between the porous concrete ecological capsule blocks with cement and wholly hinging on the top portion of the slop protection. The advantages are that solving the difficulty of the ecological repair of the hard slop protection, guaranteeing the normal growth of plants which are covered on the hard slop protection, and purifying water and increasing water landscape. The process is applied on the hard slop protection project of river and lakes, which solves the problems of plant growing stroma loss which is caused by scouring, plant growing difficulty and reducing flood control function which is caused by the improper reconstruction for the original slop protection.

The invention discloses a super-hydrophobic coating capable of being used underwater and a preparation and application method thereof, and belongs to the technical field of super-hydrophobic coatings. The super-hydrophobic coating is formed by a backing material and a hydrophobic material. The backing material is prepared from, by mass, 5% to 30% of sealing rubber, 5% to 30% of petroleum resin, 1% to 10% of flatting agent and 30% to 89% of organic solvent A. The backing material and the hydrophobic material are used in combination, and on the basis of guaranteeing super-hydrophobic performance, the backing material is utilized for increasing the direct binding strength of a super-hydrophobic coating and a base material; damage of water flow/steam and the like to the super-hydrophobic coating can be effectively reduced, use strength is increased, the super-hydrophobic state can be maintained in the underwater/high-humidity environment for a long time, the service life can be prolonged, and great significance is achieved on oceangoing voyage, underwater exploration and the like.

The invention relates to the fields of tumour physiology and biotechnology. The object of the invention is to develop effective and selective novel fusion proteins and fusion protein-antibody complexes against various types of leukaemia and solid tumours. Selectivity is achieved by cell-specific or tumour-specific ligands of the fusion proteins or by antibodies of the fusion protein-antibody complexes. Effectiveness is achieved on the one hand by the direct binding of the microtubules or cytoskeleton elements to the microtubule-binding regions and on the other hand by induction, as well as by the reinforcement of the immune reaction by regions that trigger the immune reaction on the target cells.

The present invention relates to a multi-conjugate of small interfering RNA (siRNA) and a preparing method of the same, more precisely a multi-conjugate of siRNA prepared by direct binding of double stranded sense/antisense siRNA monomers or indirect covalent bonding mediated by a cross-linking agent or a polymer, and a preparing method of the same. The preparing method of a siRNA multi-conjugate of the present invention is characterized by simple and efficient reaction and thereby the prepared siRNA multi-conjugate of the present invention has high molecular weight multiple times the conventional siRNA, so that it has high negative charge density, suggesting that it has excellent ionic interaction with a cationic gene carrier and high gene delivery efficiency.

A thermal tripping device, on a heater (1) as a fixed terminal, a bimetal strip (2) with one end as an action end (21) and the other end as a fixed end (22) The above-mentioned fixed end (22) is fastened to the above-mentioned heater (1) in a cantilever shape, and when the above-mentioned heater (1) is overheated by energizing the above-mentioned heater (1), the above-mentioned moving end of the above-mentioned bimetal (2) (21) Bending, and on the fastening part (221) where the above-mentioned bimetal strip is fastened to the above-mentioned heater, integrally and directly bond the temperature measuring member (7), which is obtained from the above-mentioned bimetal strip (2) and the above-mentioned heater (1) are exposed so as to be in contact with the contact temperature measuring device (8).

The invention discloses a method for identifying a same user. The method comprises the following steps: establishing a direct binding relationship between every two articles which are used in a combined manner; and identifying a user of all the articles which have the direct binding relationships with the same article as the same user, or identifying a user of all the articles which have the direct binding relationships and indirect binding relationships with the same article as the same user. The method provided by the embodiment of the invention has the advantage that the article user can bemore accurately identified.

The present disclosure relates to compositions and methods of modulating inflammatory and immune responses through binding of SAA to TLR2 in a subject (e.g., human, non-human primate, rodent, etc.), and compositions and methods for screening TLR2 agonists and antagonists. In the studies described herein, a potential role of SAA in neutrophilia was investigated and the results demonstrated that SAA is a potent inducer for macrophage secretion of G-CSF, which leads to neutrophilia in mice. Using G-CSF^−/− and TLR2^−/− mice, it was found that the SAA-induced neutrophilia is dependent on TLR2-mediated production of G-CSF. Based on direct binding assay and gain-of-function studies in TLR2-transfected cells, SAA was identified as a novel ligand for TLR2 and a link between increased SAA concentration and TLR2-mediated inflammatory responses such as neutrophilia was established. Additional embodiments are disclosed.

The present invention relates relates to a CRISPR-CAS system for a host cell, in particular to a method to produce a circular vector comprising one or more guide-polynucleotide expression cassettes, wherein said one or more guide-polynucleotide expression cassettes comprise a polynucleotide encoding a guide-polynucleotide operably linked to one or more control sequences which direct the expression of said guide-polynucleotide in a host cell, wherein said guide-polynucleotide comprises a guide-sequence that essentially is the reverse complement of a target-polynucleotide in a host cell and wherein the guide-polynucleotide can direct binding of a Cas protein at a target-polynucleotide in a host cell to form a CRISPR-Cas complex, in vivo.

A method for identifying a compound that has a biocidal effect against a selected organism involves screening from among known or unknown peptide or non-peptide molecules, a test molecule that binds selectively to a target sequence of a multi-helical lid of a heat shock protein of the organism. The binding of the test compound inhibits the protein folding activity of the protein. A specific embodiment of such a method is useful for identifying or designing a pharmaceutical or veterinary biocidal or antibiotic compound, preferably a pathogen and/or strain-specific compound. For this purpose, the compound does not bind to a heat shock protein that is homologous to the mammalian subject to be treated with the compound. Screening methods can encompass direct binding or competitive assays. Molecules or compounds identified by these methods are employed as biocides for pharmaceutical, veterinary, pesticide, insecticide and rodenticide uses, among others.

The present invention relates to treatment or prevention of radiation induced fibrosis using TNF-alpha antagonism. Preferably, TNF-alpha is antagonized by direct binding or by inhibition of synthesis. In a preferred embodiment, the invention comprises intraperitoneal administration of a chitosan-siRNA nanoparticle, wherein the siRNA is targeted to the mRNA encoding TNF-alpha.

The invention discloses a local device binding method. The method comprises the following steps: allocating an access address to a control device; enabling the control device to send control information containing the identification information of the access address; enabling the access address to receive the control information; enabling a controlled device to subscribe the access address; and the controlled device to acquire the control information from the access address. Compared with the prior art, the invention has the following advantages that the relates to the local binding and control technology based on the BLE mesh technology; when a remote controller or a sensor and intelligent equipment such as a lamp or a socket are in the same network, the directional control over the intelligent equipment can be achieved through direct binding of the remote controller or the sensor and the intelligent equipment, an APP can check and modify the binding relation, and a more flexible andsafer control mode is achieved; moreover, a flexible linkage control function between the sensor and the intelligent equipment is realized, the necessity of the gateway is reduced, and the cost is reduced.

The present invention provides a reinforced fiber sheet exhibiting a stable shape and favorable bulking properties, a method for manufacturing same, and a fiber-reinforced resin molded body. The reinforced fiber sheet according to the present invention comprises a plurality of reinforced fiber bundles that are arranged in one direction, wherein first and second reinforced fiber bundle layers, in which there is no direct binding force between reinforced fiber bundles within a layer, are disposed so as to have mutually distinct fiber orientations, and are integrally formed by being fastened together using fastening elements, satisfying conditions (i) and (ii). (i) Fastening surfaces of the first and second reinforced fiber bundle layers have fastening sections that include at least one fastening element, and an average surface area S1 of 100 mm2. (ii) In the fastening sections, the surface area ratio of the fastening elements to the fastening section is 0.1% to 80%, inclusive.

The invention discloses an air valve surface strengthening treatment method. The binding force between an air valve and a nitride layer and between the nitride layer and a chromium cladding layer is reinforced by first performing overall nitriding on the air valve, then performing chromium electroplating on a rod part of the air valve and finally performing diffusion annealing. According to the air valve surface strengthening treatment method, micro-cracks produced by nitriding are made up by filling the chromium cladding layer, and then Cr and N compounds are generated on the surface of the air valve through an annealing method, so that mechanical binding of the Cr and N in the micro-cracks is changed into chemical binding; therefore, the influence of the micro-cracks of the nitride layeris weakened; and the defect that the binding force between the chromium cladding layer and an air valve substrate is not strong because of direct binding in the conventional chromium electroplating method is effectively overcome.