FGFR binding peptides

A technology for peptide sequences and compounds, applied in the field of new peptide compounds, can solve the problems of direct binding and activation of FGFRs that do not show sequences

Inactive Publication Date: 2007-08-29
ENKAM PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neither of the above two applications shows direct binding of said sequence to FGFR and activation of the receptor

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0271] The preparation of polyclonal antibodies is well known to those skilled in the art. See, for example, Green et al. 1992. Production of Polyclonal Antisera in Immunochemical Protocols (Manson ed.), pp. 1-5 (Humana Press); Coligan et al., Production of Polyclonal Antisera in Rabbits, Rats Mice and Hamsters, in Current Protocols inImmunology, Section 2.4.1, which is hereby incorporated by reference.

[0272] Likewise, the preparation of monoclonal antibodies is routine. See, eg, Kohler and Milstein, Nature, 256:495-7 (1975); Coligan et al., Sections 2.5.1-2.6.7; and Harlow et al., in Antibodies: A Laboratory Manual, page 726, Cold Spring Harbor Pub. (1988). Monoclonal antibodies can be isolated and purified from hybridoma cultures by a variety of established techniques. Such separation techniques include Protein-A Sepharose affinity chromatography, size exclusion chromatography, and ion exchange chromatography, see, e.g., Coligan et al., Sections 2.7.1-2.7.12 and Sectio...

Embodiment 1

[0417] Example 1 Stimulation of neurite outgrowth

[0418] Cerebellar granule neurons (CGN) were prepared from postnatal 7 day Wistar rats essentially as described previously (Neiiendam et al., (2004) J Neurochem. 91(4):920-35). Cerebellar tissue was dissected in modified Krebs-Ringer solution kept on ice and processed as described to obtain upper hippocampal neurons. All cell cultures were maintained at 37 °C with 5% CO 2 Incubate under a humidified atmosphere. All animals were handled in accordance with national guidelines for animal welfare.

[0419] Dissociated cells at 10,000 cells / cm 2Spread on uncoated 8-well permanox Lab-Tek culture slides (chamber slides), add 0.4% (w / v) bovine serum albumin (BSA; Sigma-Aldrich), 2% (v / v) In Neurobasal medium with B27 Neurobasal supplement, 1% (v / v) glutamax, 100 U / ml penicillin, 100 μg / ml streptomycin and 2% 1M HEPES (all from Gibco, BRL). Add peptide solutions with or without multiple signal transduction pathway inhibitors to 3...

Embodiment 2

[0421] Example 2 Stimulation of Neuron Survival

[0422] Survival assay

[0423] The primary cultured CGN was cultured at 100,000 cells / cm 2 The density was spread on polylysine-coated 8-well permanox slides, added with 2% (v / v) B27, 0.5% (v / v) glutamax, 100U / ml penicillin, 100μg / ml streptomycin and In Neurobasal-A medium (Gibco, BRL) with KCl, the final concentration of KCl in the medium was 40 mM. 24 hours after plating, cytosine-β-D-arabinofuranoside (Ara-C; Sigma-Aldrich) was added to a final concentration of 10 μM to avoid glial cell growth, and neurons were allowed to redifferentiate at 37°C for 6 days. Wash 2 times and change medium to supplemented with 1% (v / v) glutamine, 100 U / ml penicillin and 100 μg / ml streptomycin, 3.5 g D-glucose / l and 1% (v / v) acetone Sodium bicarbonate (Gibco BRL) and Eagle's minimal medium (BME; Gibco BRL) with different concentrations of peptides were used to induce apoptotic cell death. The potassium concentration in the culture was thus ...

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Abstract

The present invention relates to new peptide compounds capable of direct binding to fibroblast growth factor receptor (FGFR) and activating said receptor. The compounds of the invention comprise peptide fragments of the neural cell adhesion molecule (NCAM) derived from the fibronectin type-III module 1 (F3, 1) of NCAM. Peptide sequences of the invention are capable of stimulating learning and memory and / or neurite outgrowth and / or neural cell survival. Peptide sequences and compounds comprising thereof, according to the invention, may be beneficially used for treatment and / or prevention of different pathological conditions wherein FGFR and / or NCAM play a role in pathology and / or recovery from disease. Accordingly, pharmaceutical compositions comprising the peptide sequences and compounds of the invention are also in the scope of protection.

Description

field of invention [0001] The present invention relates to novel peptide compounds derived from the sequence of fibronectin type III module 1 (F3,1) of neural cell adhesion molecule (NCAM), which bind to fibroblast growth factor receptor (FGFR). The invention also relates to pharmaceutical compositions comprising the compounds and their use for the treatment and / or prevention of different pathological conditions in which FGFR and / or NCAM play a role in pathology and disease recovery. Background technique [0002] Brain plasticity (brain plasticity) and the mechanisms that control plasticity have important implications for learning and memory, as well as functional recovery after brain injury. It is well known that neurotrophic factors are one of the molecular groups that continuously regulate brain plasticity in the adult central nervous system (CNS), but cell adhesion molecules (CAMs) play a more important role in, for example, long-term potentiation, neuronal preservation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/705C07K16/28A61K38/17A61P25/28C07K7/00
CPCC07K14/70503A61P25/14A61P25/16A61P25/18A61P25/28A61P35/00
Inventor 伊莎贝思·博克弗拉迪米尔·贝雷津
Owner ENKAM PHARMA
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