159 results about "Animal welfare" patented technology

The invention relates to a cultivation method of high-grade beef cattle. The cultivation method is characterized by comprising the following steps: carrying out nutrition regulation of concentrated and coarse feed and fence alternating feeding according to month periods of growing periods of the beef cattle; feeding six to seven heads of beef cattle in every two adjacent small fences; feeding the beef cattle in one fence while the other fence is empty; alternating the fences once every seven to ten days according to the humidity of padding on the ground surface of the fences; circulating the feeding process until the beef cattle are finally on the market. The coarse feed is formed by mixing 50% of ensiling corn stalks and 50% of fresh brewer's grain. The concentrated feed mainly consists of corn and maize embryo cakes, as well as bran, cottonseed cakes, microelement premix and the like. The nutrition regulation meets the requirements of the beef cattle on bones, rumens, muscles and fat deposition in each growing period furthest, and the table purpose performance indexes of beef cattle daily gain, slaughter performance, high-quality meat loaf yield ant the like are improved. However, the animal welfare is improved through the fence alternating feeding, the table purpose performances are improved, and the average weight of the beef cattle can reach to 850-900 kg.

The present invention concerns an anaerobic digestion of animal manures, energy crops and similar organic substrates. The process is capable of refining nutrients comprised in the digested biomass to fertilizers of commercial quality. The invention also provides a method for oprocessing animal carcasses or fractions thereof including meat and bone meal etc., with the objective of providing an alternative means for processing the organic waste material of animal origin while at the same time facilitating the production of fertilizers. The risk of spreading BSE prions or any other prions to animals or humans is thus substantially reduced if not eliminated. The biogas and slurry separation system according to the present invention is preferably integrated with the operations of animal husbandries into a total concept in which the internal and external performances of animal husbandries are optimised. The internal performances concern quality aspects related to the management of the animal houses and include industrial hygiene, animal welfare, gaseous and dust emissions and food safety. The external performances concern mainly energy production and emissions to the environment of nutrients and greenhouse gases and the sale of high quality food product.

The invention belongs to the technical field of stockbreeding machines, and particularly relates to a living thing and environment monitoring intelligent robot. The living thing and environment monitoring intelligent robot comprises a ball screw control mechanism, a knocking mechanism, a reversing mechanism, a walking mechanism, a temperature and humidity environment monitoring system and a dead or sick chicken recognition system. The situation that in the poultry cage breeding mode of China, the dead or sick chickens have to be recognized through manual work and the environment has to be monitored through manual work becomes history, the labor intensity of breeding workers can be greatly lowered, the working conditions and animal welfare of the workers are improved, the intelligent and automation level of the livestock industry is increased, the comprehensive economic benefits of the large-scale chicken breeding industry are improved, the technical guarantee is provided for the sustainable and healthy development of the laying hen breeding industry, another bottleneck in the intensive breeding field is broken through, and the extremely-high industrial value and the extremely-high application value are achieved.

The present invention concerns an anaerobic digestion of animal manures, energy crops and similar organic substrates. The process is capable of refining nutrients comprised in the digested biomass to fertilizers of commercial quality. The invention also provides a method for processing animal carcasses or fractions thereof including meat and bone meal etc., with the objective of providing an alternative means for processing the organic waste material of animal origin while at the same time facilitating the production of fertilizers. The risk of spreading BSE prions or any other prions to animals or humans is thus substantially reduced if not eliminated. The biogas and slurry separation system according to the present ivnention is preferably integrated with the operations of animal husbandries into a total concept in which the internal and external performances of animal husbandries are optimised. The internal performances concern quality aspects related to the management of the animalhouses and include industrial hygiene, animal welfare, gaseous and dust emissions and food safety. The external performances concern mainly energy production and emissions to the environment of nutrients and greenhouse gases and the sale of high quality food product.

The invention discloses a production method for promoting quality of cooled fresh-keeping beef. The method consists of the following steps: 1, implementing animal welfare in the processes of breeding, transporting and processing beef cattle so as to effectively reduce the stress reaction of live cattle; 2, adopting two-stage acid discharge, wherein precooling is performed for 2 hours in a rapid precooling room at an ambient temperature of 15 DEG C below zero in a first stage of acid discharge; and in a second stage of acid discharge, ozone fumigation is performed for 2 hours in acid discharge warehouse to effectively control surface microorganisms of acid discharge rooms and carcasses, and precooling is performed for 36 hours at an ambient temperature between 2 DEG C below zero and 2 DEG C and relative humidity between 85 and 90 percent; 3, effectively implementing an HACCP system in the processing process to control physical, chemical and biological hazards and improve product quality; and 4, applying vacuum thermal-shrinkage packaging technology to enable the shelf life to reach more than 90 days and applying air-conditioned fresh-keeping technology to reduce the exudation of bloody water in cooled fresh-keeping beef and enable the shelf life to reach more than 20 days.

The physiological regulation of intake, growth and energy partitioning in animals is under the control of multiple genes, which may be important candidates for unraveling the genetic variation in economically relevant traits in beef production. The present invention relates to the identification of single nucleotide polymorphisms (SNPs) within the bovine gene encoding mitochondrial transcription factor A (“TFAM”) and their associations with economically relevant traits in beef production. The invention further encompasses methods and systems, including network-based processes, to manage the SNP data and other data relating to specific animals and herds of animals, veterinarian care, diagnostic and quality control data and management of livestock which, based on genotyping, have predictable meat quality traits, husbandry conditions, animal welfare, food safety information, audit of existing processes and data from field locations.

A highly automated system to identify, measure, manage and control individual animals in grazing environments to monitor, analyze, model, predict, promote, optimize, and mitigate a variety of conditions and interactions relating to the grazing environment and an animal's health, welfare, performance, productivity, efficiency, quality, economic and genetic value. The system having a transmitters that identify individual animals. A weighing device that weighs the animal while the animal consumes substances and a dispensing device that controls substance provision to individual animals. A computer considers a number of factors in generating control signals sent to the system to dispense a prescribed amount of mineral, vitamin, medicinal or supplement compound to an individual animal. The system models, predicts and estimates a number of conditions related to the animal and its grazing environment that improve animal welfare, increases production, and preserves and conserves resources.

The physiological regulation of intake, growth and energy partitioning in animals is under the control of multiple genes, which may be important candidates for unraveling the genetic variation in economically relevant traits in beef production. The present invention relates to the identification of single nucleotide polymorphisms (SNPs) within the bovine genes encoding fatty acid binding proteins and their associations with economically relevant traits in beef production. The invention further encompasses methods and systems, including network-based processes, to manage the SNP data and other data relating to specific animals and herds of animals, veterinarian care, diagnostic and quality control data and management of livestock which, based on genotyping, have predictable meat quality traits, husbandry conditions, animal welfare, food safety information, audit of existing processes and data from field locations.

The invention discloses an ecologically environmentally-friendly live pig feeding method and a special colony house thereof. The live pigs are bred in the special colony house. The special colony house is characterized in that an ecological fermenting bed is manufactured in the colony house. The manufacture method for the ecological fermenting bed comprises the following steps of: (1) mixing and evenly stirring saw dust, loess, crude salt, EM bacteria, brown sugar and water at the mass ratio of 65: 10: 0.25: 0.25: 0.25: 25 to obtain the coarse material of the fermenting bed; (2) stacking and fermenting the above coarse material of the fermenting bed for 4-6 days to obtain fermenting bed padding; and (3) laying down the fermenting bed padding in a pigsty, wherein the total thickness of the padding is about 80-100cm, and the padding of each square meter weighs about 200kg. The ecologically environmentally-friendly live pig feeding method is a set of virtuous-circle ecological breeding system built by combining culture technology, microbiology, animal nutrition, environmental hygiene with soil fertilizer science and combining animal welfare into a whole. The adopted fermenting raw material bed has the advantages of wide material resource, low cost and simple manufacture method and is convenient to popularize and apply.

The invention discloses experimental animal particle litter added with Chinese herbal medicine and prepared from straw and a preparation method and application of experimental animal particle litter. The experimental animal particle litter added with the Chinese herbal medicine is prepared by mixing and granulating, by mass, 85%-90% of straw mixture, 0.01%-1% of Chinese herbal medicine, 7.5%-13% of bentonite and 0.04%-1.5% of activated carbon. The straw mixture comprises one or more of wheat straw, rice straw, corn straw, potato straw, oilseed rape straw, cotton straw and sugarcane straw. The Chinese herbal medicine is composed of at least two of cortex pseudolaricis, chrysanthemum flowers and wormwood. The detecting result of the particle litter completely meets the quality standard, the living environment of experimental animals is obviously improved, animal welfare is improved, and the labor intensity of a breeder is reduced. Environmental pollution is reduced, and environment protection is promoted.

The invention provides a quantitative feeding box which can realize automatic feeding and intelligent food adding according to physiological feeding needs of animals. The quantitative feeding box comprises a feeding plate and a feed trough, wherein the feed trough is connected with the feeding plate through a downcome pipeline, an electromagnetic valve is arranged between the feed trough and the downcome pipeline, a weight sensor and a pressure sensor are mounted on the feeding plate and are electrically connected, and the pressure sensor is electrically connected with the electromagnetic valve. The quantitative feeding box is simple in structure, can be applied to the feeding process of experimental primate animals, and particularly has positive significance on comparative research about improvement of diversification and interestingness of animal feeding action, enrichment of animal welfare, reduction of feeding frequency and human disturbance as well as quantitative analysis of nutritional requirements and feeding nutrition conditions of the animals.

The invention provides a fast shock handling device for small animals through an inert gas. The fast shock handling device comprises an integrally sealed handling cabin for forming a sealed oxygen-deficient environment through opening and automatic closing of a cabin top sliding door; a program configuration operation panel, a program control circuit and a data display window are used for configuring operation programs and are matched with an inert gas sensor, an oxygen concentration sensor and a pressure sensor to finish device control and data display together; an inert gas source is controlled by an electric control flow valve and an electric control inlet valve to release excess gases in the entering process of the inert gas; and a negative pressure air valve is matched with a negative sucking pump and is communicated with a handling cabin to replenish fresh air into the handling cabin in time. With the adoption of the fast shock handling device. fast handlings can be safely and conveniently carried out on shock and euthanasia without scare, injury and pain in scientific researches, the rights of the animals can be and maximally defended, and the technical problem in the aspects of animal welfare is solved through more humanistic and scientific devices and methods; and the fast shock handling device is widely applied in scientific researches.

Pellets containing an analgesic uniformly dispersed in a lipid carrier such as cholesterol mixed with fatty acid esters, can be used to provide long term pain relief. 5 mg cholesterol-tryglyceride-buprenorphine pellets released the majority of drug in 24-48 hours after implant and provide clinically significant plasma levels of analgesia in mice for 3-9 days. Blood levels of analgesia peak at day-1 and are substantially complete by day-5 depending on the level of buprenorphine. These results demonstrate that post surgical implants provide clinically significant levels of analgesia in the 24-48 hour period following surgery and thus obviate the time consuming, expensive, and high-risk need to inject mice post surgery. The pellets are safe and easy to use. Placed in the surgical wound at the end of surgery, they provide 2-3 days of analgesia and obviate the need for subsequent handling of the animal for pain therapy. The implants have no detectable effect on mouse behavior, hematology, or liver chemistry. The unexpected release kinetics of the 5 mg pellet provides an ideal implant for post surgical analgesia. These implants solve a significant problem facing scientists who use rodents in research and abide by international of animal welfare.

The invention discloses a method for producing pollution-free flavoured chilled pork, which belongs to raising processing method of livestock food and solves the problem that the quality of pork is not high and the tastiness is not good because of the disadvantages existing in the prior live pig raising and slaughtering technology. Animal welfare measures are brought in the two processes of raising and slaughtering processing, the ablactation time for piglets is increased to 28 days during the raising process, and dedicated cereal crops with full nutritional value is prepared, wherein, flavorenhancers are added in the dedicated cereal crops; light music is played; a temperature regulation and control device (comprising a heater and a water sheet cooling system) is arranged in a hog house; the herd management density that allows the activity space of at least 2 square meters for each pig is ensured; small toys are provided so as to satisfy foraging and searching behavior habits of thepigs; in addition, rest, feeding and water supply are ensured when the pigs are transported during the slaughtering and manufacturing process, the pigs enter a slaughter house when hearing the light music and are slaughtered through electric shock in an isolating manner, and then condensation, steaming, scalding, carcass opening, disemboweling, halving, cooling, refrigeration, acid discharge and examination are carried out. The method is suitable for batch production of commercial pork.

An antimicrobial solution for disinfecting or removing micro-organisms from a surface used by animals or for use as an animal shampoo is described. The solution is active against bacteria, fungi, and viruses, including enveloped viruses, and skin disorders related to insect infestation.

The invention discloses a toy-type automatic excrement cleaning system for a pig. The toy-type automatic excrement cleaning system comprises a toy-type protection base for loading an excrement cleaning robot and the excrement cleaning robot, wherein the opening and closing function of the toy-type protection base can be achieved, and protection of the excrement cleaning robot is achieved. In addition, a camera is installed at the highest portion of the midpoint of the inner side of a pig house to identify positions of the pig, excrement and the robot, planning of an optimal path is carried out, and the excrement cleaning robot can efficiently clean treated excrement; the excrement cleaning robot is guided to effectively avoid obstacles through an ultrasonic distance measuring sensor; a non-working-state excrement cleaning robot is protected by arranging the robot protection base, the pig is excited to play with a toy for a long time, and healthy growth of the pig is facilitated. By means of the toy-type automatic excrement cleaning system for the pig, real-time cleaning of pig-farm excrement can be achieved, the system has the advantages of being capable of saving time and labor and efficient, and the animal welfare requirement is also met.

The invention discloses a non-fasting forced molting method for laying hens, belongs to the technical field of forced molting methods for poultry in poultry raising, and provides a non-fasting forced molting method in order to find a feasible method for inducing molting of poultry instead of a starvation method. The method specifically includes the steps that hens are fed with molting feed in the molting implementation stage, the daily feed intake amount of each hen is controlled to range from 40 g to 55 g, the temperature of a henhouse is controlled to range from 18 DEG C to 25 DEG C, and the light illumination is controlled to be 8 h / d; the hens are fed with recovery stage feed and can eat feed freely in the molting recovery stage, the temperature is controlled to range from 20 DEG C to 27 DEG C, and the light illumination is 16 h / d. According to the method, stress on the hens is small, the death and culling rate of the hens is small, the method meets the animal ware requirement, meanwhile, the laying rate rises again very fast after the molted laying hens start to lay with the method, and the method is a laying scheme which is scientific in management, high in cost effectiveness and sustainable and can be widely applied.

The invention discloses a method for relieving piglet weanling stress by using simulated sow placatory voice, comprising the steps of: synchronously recording behaviours of piglets and sows and the hum of the sows in the suckling circulating process from the previous suckling ending to the next suckling ending of the piglets by using a camera; then selecting segments of the sows calling the piglets before the milk releasing by using audio software, separating videos from audios, denoising the audios by using audio editing software, and superposing and amplifying the obtained audios to obtain video segments; adding silence segments at the ends of the video segments to obtain placatory voice files; and repeatedly playing in piglet nursery sites by using a playing device. The invention also discloses a weaning piglet sow voice comforting machine of playing equipment. According to the method disclosed by the invention, the piglet weanling stress can be reduced, the piglet animal welfare level is improved, the piglet growing speed is increased, and the sow breeding period is shortened.

The present invention concerns an anaerobic digestion of animal manures, energy crops and similar organic substrates. The process is capable of refining nutrients comprised in the digested biomass to fertilizers of commercial quality. The invention also provides a method for processing animal carcasses or fractions thereof including meat and bone meal etc., with the objective of providing an alternative means for processing the organic waste material of animal origin while at the same time facilitating the production of fertilizers. The risk of spreading BSE prions or any other prions to animals or humans is thus substantially reduced if not eliminated. The biogas and slurry separation system according to the present invention is preferably integrated with the operations of animal husbandries into a total concept in which the internal and external performances of animal husbandries are optimized. The internal performances concern quality aspects related to the management of the animal houses and include industrial hygiene, animal welfare, gaseous and dust emissions and food safety. The external performances concern mainly energy production and emissions to the environment of nutrients and greenhouse gases and the sale of high quality food product.

The invention relates to the technical field of agriculture culture, in particular to a pig raising fermentation bed packing and a preparation method thereof. The packing contains the following components: soil, wheat bran, rice bran, peanut shells, plant straws, soybean meal, sawdust, traditional Chinese medicine components, table salt, and fermentative strains. The preparation method comprises the following steps: (1) crushing the peanut shells, the plant straws, the soybean meal, and the traditional Chinese medicine components respectively for later use; and (2) mixing the components in proportion, adding an appropriate amount of water, and stirring the components uniformly to obtain the fermentation bed packing. The pig raising fermentation bed packing provided by the invention mainly makes use of crop straws and leftovers obtained by agricultural product processing, so that the raw material cost is greatly reduced, the material utilization rate is improved, the animal welfare is improved, and a positive cycle between agriculture and animal husbandry is achieved; and the preparation method is simple and easy to operate, and has a relatively good application value.

The present invention discloses an animal welfare facility, in particular to an anti-stress machine used for a pig which is arranged in a hutch for meeting the demand of such normal actions as rooting and bite and so on of pigs, reducing the fight and attack actions between pigs and bringing down swine herd stress. The technic proposal comprises a cavity (1) for rooting of the pig which is provided with a rope (3) for the bite of the pig; the cavity is also provided with a suspension ring (2); materials for emitting smell are arranged in the cavity (1). The present invention has the advantages that the invention is firm and durable, easy to be cleaned and disinfected and is able to be produced in large scale, moreover, the invention is easy to be discerned by pigs, which attracts the pigs to study and play for long time. The use of the facility reduces the abnormal actions of pigs and the attack to the fellows, reduces the play and damage of pigs to the feeding facility and increases the harmony of swine herd, thus being beneficial to improving production performance.

The physiological regulation of intake, growth and energy partitioning in animals is under the control of multiple genes, which may be important candidates for unraveling the genetic variation in economically relevant traits in beef production. The present invention relates to the identification of single nucleotide polymorphisms (SNPs) and their haplotypes, across the bovine genome in genes encoding polypeptides associated with feed efficiency, and their associations with residual feed intake in beef production. The invention further encompasses methods and systems, including network-based processes, to manage the SNP data, haplotype data and other data relating to specific animals and herds of animals, veterinarian care, diagnostic and quality control data and management of livestock which, based on genotyping, have predictable meat quality traits, husbandry conditions, animal welfare, food safety information, audit of existing processes and data from field locations.

The invention provides a method for separating cells from blood and cultivating the cells and a method for cloning non-human animals. The method for separating cells from blood and cultivating the cells comprises following steps: (1) adding the blood to an upper layer of a separation liquid and performing centrifugation in a manner of density gradient centrifugation for obtaining mononuclear cells; (2) inoculating the mononuclear cells onto an in-vitro cultivation device containing an adherent cell culture medium to obtain adherent cells; and (3) performing serial subcultivation to the adherent cells to obtain somatic cell nuclear transplanted donor cells. The somatic cell nuclear transplanted donor cells obtained through the method can be directly used for manually cloning the non-human animals. The method is simple in operation processes, is easy to carry out, is low in cost, is less in stress stimulation and harm in animals, and can ensure a complete appearance of the animals and improve animal welfare.

The invention relates to the technical field of livestock and poultry ingestion behavior prediction equipment, and discloses a pig ingestion behavior prediction method and device, and the method comprises the steps: obtaining an image of a pig in a ingestion device, inputting the image of the pig into a preset pig behavior discrimination model, and outputting a pig ingestion state result; whereinthe preset pig behavior discrimination model is obtained through training by taking pig image sample data as a first training set and taking a pig ingestion state corresponding to the pig image sampledata as a label. According to the method, the pig ingestion behavior is predicted in a quantitative mode, the reliability and the accuracy of a prediction result are ensured, the reliability and theaccuracy of the prediction result are ensured, the pig ingestion behavior is accurately predicted and recorded under the condition that animal welfare is not affected, the pig ingestion amount can bebetter controlled, and the production efficiency is improved.

The invention discloses a novel henhouse. Frame plates are laid on the left side and the right side in a henhouse body, a pedestrian passageway is arranged between the frame plates on the two sides, chickens in the henhouse can drink water through a nipple type water dispenser, and water resource waste and drinking water pollution can be avoided; a chicken feed is conveyed into a feed tray throughrotation of a plug tray, feed conveying is convenient, the labor cost is low, and the feed is not classified; the requirements of natural release and animal welfare of chickens can be met through a sand bath tray and a perching frame, and chicken manure can fall into manure scraping rails below through the frame plates; the chicken manure in the manure scraping rails can be scraped into a manurecollecting tank by controlling a manure scraping mechanism through a control mechanism; and then the chicken manure in the manure collecting tank is conveyed to the outside of the henhouse body through a spiral packing auger, the chicken manure can be conveyed even a manure conveying vehicle does not enter a chicken farm, the chicken manure is easy and convenient to convey, and the probability ofchicken infection in the henhouse is small. Meanwhile ventilation in the henhouse can be achieved through a draught fan on the inner side wall of the henhouse body.

The invention belongs to the technical field of poultry sex detection, and in particular relates to a duck embryo early sex identification method and a special kit. The duck embryo early sex identification method is established by collecting a hatched early duck embryo allantoic fluid, making use of a monoclonal antibody for estradiol detection and carrying out antigen-antibody reaction. Male and female sexes are judged according to a characteristic that the content of estradiol in a female embryo is higher than 1ng / ml and the content of the estradiol in a male embryo is lower than 0.6ng / ml. The identification method disclosed by the invention can identify the female and male sexes in an early hatching period without an influence on the further development of the embryo; and the identification method is conducive to animal welfare and relieving the transmission of vertical diseases.

The invention discloses a beef cattle breeding method. The beef cattle breeding method comprises the steps that fine and coarse feed nutrition regulation and rail rotation feeding are carried out in month age stages according to the growth periods of beef cattle, wherein according to rail rotation feeding, one group of cattle are fed in every two adjacent small rails, five cattle are included in each group, the other rail is empty while cattle are fed in one rail, rotation of the rails is carried out once every 7.5 days according to the humidity condition of the ground padding of the rails, and the operation circulates in the whole feeding process until fattening of beef cattle is completed for slaughter finally. According to the beef cattle breeding method, the meat performance of high-grade beef cattle is improved with the methods of nutrition regulation, animal welfare improvement and the like.

The invention discloses a chicken egg yolk antibody resisting human A rotavirus as well as a preparation method and application thereof. The method comprises steps of expressing and purifying structural protein VP7 or VP8 of human rotavirus in vitro, immunizing laying hens, collecting eggs, and separating and purifying egg yolk antibody of VP7 or VP8. The prepared chicken egg yolk antibody can be freeze dried and prepared into antibody dry powder for preventing and treating rotavirus. The preparation method has the beneficial effects that a large quantity of VP7 and VP8 antigens are expressed and recombined in vitro by a genetic engineering technology, the benefits are high, the cost is low, small field is required, and the method is environment-friendly; the potential risk of scattering virus caused by inactivation by rotavirus or attenuated vaccine in the traditional preparation process of egg yolk antibody is avoided, and the method meets the requirement of animal welfare. The egg yolk antibody prepared by the method can be prepared into oral antibody dry powder biological agent after sterility test, has obvious effect in treating rotavirus infectious diarrhea, takes effect rapidly and has low cost, and the prevention rate reaches over 95%.

The invention belongs to the technical field of livestock medical treatment, and particularly relates to a piglet hernia surgery restraint frame. The piglet hernia surgery restraint frame comprises a restraint frame body. The restraint frame body is a cuboid frame body, the length of the restraint frame body ranges from 80 mm to 100 mm, the width of the restraint frame body ranges from 25 mm to 30 mm, the height of the restraint frame body ranges from 60 mm to 70 mm, a U-shaped top plate is arranged on the top of the rectangular frame body, the total height of the top plate ranges from 10 mm to 12 mm, at least three second annular buckles are evenly and fixedly arranged on the back face of the top plate, the second annular buckles are connected to the front face of the top plate through second sticky buckles, four supporting frames perpendicular to the top plate are further movably arranged on the top plate, the length of the supporting frames ranges from 40 mm to 45 mm, the four supporting frames are symmetrically distributed on the front face and the back face of the top plate, the distance between the two supporting frames on the same side ranges from 26 mm to 35 mm, at least one first annular buckle is fixedly arranged on the left face of each supporting frame, and the first annular buckles are connected to the right faces of the supporting frames through first sticky buckles. According to the piglet hernia surgery restraint frame, manpower and physical power can be saved, restraint is quite stable, the surgery is conveniently performed, and the piglet hernia surgery restraint frame conforms to the animal welfare principle.

The invention relates to a preparation method of a classical swine fever spleen-lymph-sourced compound living vaccine and belongs to the technical field of veterinary biological products. The preparation method of the classical swine fever spleen-lymph-sourced compound living vaccine comprises the following process steps: selecting a domestic rabbit to be inoculated; inoculating; measuring the temperature and observing; harvesting; preparing virus suspension and inspecting; carrying out mixed adsorption on the harvested full-suspension virus bulk and chicken anti-hog cholera virus egg yolk antibody; and adding freezing and drying protecting agent containing an immunopotentiator into antigen antibody compound obtained through adsorption, carrying out split charging, freezing, and carrying out vacuum drying, so that the classical swine fever spleen-lymph-sourced compound living vaccine is obtained. By adopting the preparation method of the classical swine fever spleen-lymph-sourced compound living vaccine, an antibody acquiring route is simple, animal welfare can be improved, manpower and material resources are saved, production cost is greatly reduced, humoral immunity of the living vaccine can be greatly improved, and high neutralizing antibodies can be produced; and the classical swine fever spleen-lymph-sourced compound living vaccine can be applied to piglet immunization, maternal antibody interference can be avoided, no interference is produced to other vaccines, especially a mycoplasma hyopneumoniae vaccine and the like, after the classical swine fever spleen-lymph-sourced compound living vaccine is applied to piglets, and strong mucosal immune response can be induced, so that protection rate is improved.