The invention discloses an
adjuvant for animal vaccines, and a preparation method and application thereof. The
adjuvant for animal vaccines comprises the following raw materials by volume: 75 to 90% of low-density
mineral oil, 1 to 10% of octyl and decyl glycerate and Span-80, 0.5 to 5% of glycerin and polyglycerol isostearate and 1 to 10% of
polyethylene glycol-12-hydroxystearate-15, Tween-80 and
propylene glycol, wherein the amount of Tween-80 is in a range of 0 to 5%. The preparation method comprises the following steps: weighing the low-density
mineral oil; then adding octyl and decyl glycerate, Span-80, glycerin, polyglycerol isostearate, 1 to 10% of
polyethylene glycol-12-hydroxystearate-15, Tween-80 and
propylene glycol; and carrying out mixing and standing. The
adjuvant provided by the invention has safety, stability and self-emulsifying performance, has low sensitivity to temperature in the process of emulsification, can form a water-in-oil-in-water
spatial structure, and is extensively applicable to preparation of animal vaccines.