12 results about "Egg masses" patented technology

The application relates to the field of microbial technology and discloses a Pseudomonas asiatica HNHMPs-260 and application thereof. The strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66854. Fermentation supernatant of the strain can prevent and treat root-knot nematode disease of Hami melon caused by Meloidogyne javanica. Experiments show that the fermentation supernatant has strong toxic effects on second instar larvae of root-knot nematodes and can significantly inhibit the hatching of egg masses and egg grains. In addition, the fermentation supernatant has good thermal stability and storage stability, and still maintains more than 80% nematocidal activity after high-temperature treatment at 121 DEG C and long-term storage at 4 DEG C. Meanwhile, the fermentation supernatant of the strain can promote the growth of Hami melon plants, improve physiological indexes such as root length, stem length, fresh weight and dry weight, and has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.

The application provides a kind of for apple snail egg mass eradication collection device and its use method, it is related to egg mass eradication collection technical field, including shovel seat, telescopic rod is provided above shovel seat, telescopic rod outside is equipped with handheld rod, the front end surface of shovel seat is provided with auxiliary fitting component, the lower end surface of shovel seat is provided with fixing frame, rotation adjusting component is arranged between shovel seat and telescopic rod, by the design of auxiliary fitting component, the shovel head can be self-adapting shrink, so that better adhesion can be obtained when facing curved surface or irregular surface, by the setting of rotation adjusting component, shovel seat can be rotated in horizontal direction and vertical direction relative to telescopic rod to a certain extent, so that the egg mass in different positions can be conveniently eradicated by user.

The present invention provides a method for producing artificial seeds of whelks that enables year-round production by hatching larvae from egg masses obtained through spawning and implementing appropriate management according to larval development stages, thereby reducing mortality rates and increasing production yields. By managing hatched larvae according to early, middle, and late stages, it is possible to reduce mass mortality occurring during the artificial seed production process. Furthermore, by analyzing the correlation between the installation of chillers and the replacement cycle of housing filters for water quality control and water purification efficiency, it is possible to prevent the influx of external pathogens (ciliates, scuuchica) caused by rapid environmental changes during periods of high water temperature. Additionally, by artificially inducing larval settlement, the metamorphosis period can be shortened and growth variations between individuals can be reduced. Moreover, it is possible to establish a complete whelk aquaculture system that progresses from seed production to intermediate rearing to main rearing.

This invention belongs to the field of biotechnology, specifically relating to the acetylcholine receptor of the root-knot nematode *Eupolyphaga sinensis*. unc38 Genes, their dsRNAs, and applications. To provide novel target genes for the control of *Eriocheir sinensis* root-knot nematode, this invention is based on the *Eriocheir sinensis* acetylcholine receptor... unc38 Gene SEQ ID NO:1 was used to design and synthesize gene fragment SEQ ID NO:5 and the corresponding dsRNA. The synthesized dsRNA was used to soak second-instar root-knot nematodes. unc38 Gene expression levels were downregulated, resulting in a mortality rate of 68.9% at 60 hours, and complete death of nematodes at 120 hours. Immersion unc38 The dsRNA-infected second-instar larvae of the bean root-knot nematode significantly reduced the infectivity of tomato roots. After 30 days of inoculation, the number of root knots decreased significantly by about 57%, and the number of females, the number of egg masses, the number of eggs in each egg mass, and reproductive factors were all significantly reduced.

The utility model relates to the technical field of entomology, in particular to a prodenia litura egg mass collecting device which comprises a collecting disc base, a collecting disc mechanism is arranged on the upper surface of the collecting disc base, and a miniature blowing mechanism is arranged on the upper surface of the collecting disc mechanism. According to the prodenia litura egg mass collecting device, the flexible egg taking head is adopted and matched with the negative pressure adsorber to collect egg masses, the physical integrity and biological activity of the egg masses are greatly improved, the spraying head on the flexible egg taking head can spray substances to reduce the viscosity between the egg masses and host plants or gauze, and the egg masses can be more easily collected; the loss of the egg masses in the collecting process is reduced, meanwhile, wind power can be adjusted through a miniature blowing mechanism, scale hair on the surfaces of the egg masses can be effectively cleaned, an air spraying opening of an air flow distribution cavity corresponds to a storage sleeve, scale hair cleaning can be accurately conducted on each egg mass, the cleaned scale hair falls into a scale hair storage chamber, pollution to the experimental environment is avoided, and the experimental efficiency is improved. And the accuracy of subsequent egg mass counting is greatly ensured.

The invention belongs to the technical field of coleoptera bean weevil subfamily insect breeding, and particularly relates to a gleditsia sinensis bean weevil sectional type artificial breeding method based on host preference, and a breeding device comprises an egg period insect breeding box, a double-layer insect breeding box connecting table, an adult egg laying box, a hollow attractant storage box, an opening gauze box cover and a top gauze box cover; mature and undamaged wild gleditsia sinensis seeds are selected and put into a larva feeding box, gleditsia sinensis bruchid eggs are inoculated, larvae and pupae develop in the gleditsia sinensis seeds, eclosion adults are transferred into an oviposition box, gleditsia sinensis seeds are put into the oviposition box, the adults naturally mate and oviposition, egg particles are collected and transferred into a new larva feeding box, and after the eggs are hatched, the gleditsia sinensis bruchid eggs are put into the new larva feeding box. The gleditsia sinensis seeds are replaced with gleditsia sinensis seeds for larva stage breeding according to the step 4, and circulation breeding is achieved. The method is easy and convenient to operate and low in cost, the survival rate of the larvae and the egg laying amount of the imagoes can be remarkably increased, large-scale continuous breeding of the gleditsia sinensis bruchid is achieved, and a stable and high-quality insect source is provided for scientific research tests.

The utility model discloses a luring screw egg mass cleaning device which comprises a lower rod body, a luring screw body is installed at the top end of the lower rod body, and a moving structure comprises a tape spring installed at the top end of the luring screw body and an installation ring installed at the top end of an installation rod. The tape spring is installed at the top end of the luring screw body, the bottom end of the nylon wire is firstly downward and then penetrates through the fixed pulley, the nylon wire is changed to be upward to be connected with the installation ring, the installation ring is fixed to the installation rod, when the installation ring moves to the top end of the luring screw body, the tape spring can be tightened, and the buckle can clamp the installation block at the top end of the installation rod. After a pressing block is pressed, a buckle does not clamp a mounting block any more, the elasticity of a tape spring can drive a nylon wire to be recycled, and when the nylon wire is recycled, a cutting circular ring can be driven to move downwards to cut egg masses on the inducing screw body, so that the purpose that the inducing screw egg mass cleaning device conveniently and automatically cleans the egg masses is achieved.

The invention relates to the technical field of medicinal breeding of special insects, in particular to a method for ecologically and cyclically domesticating high-quality maggot and a domesticated population thereof, and the method comprises the following steps: seed source acquisition and identification: acquiring chrysomyia megacephala adults, performing purebred identification and health detection, and pre-screening an initial seed source; hatching eggs of the obtained provenance in an artificial environment, feeding larvae of 1-3 years old, applying primary environmental stress, and screening individuals; putting imagoes into the natural environment after emergence of the individuals, and recovering spawning blocks; the recovered egg masses are hatched and fed with larvae of 1-3 years old, composite environmental stress is applied, and individuals with tolerance reaching the standard are screened out; after the individuals emerge adults, putting the adults into a complex natural environment, and recovering the spawn blocks; the recovered egg masses are hatched and fed with larvae of 1-3 years old, ultimate environmental stress is applied, and domesticated populations with solidified characters are obtained through screening. Through three-generation directional domestication, artificial stress, natural energizing and fresh fish waste utilization, the effects of pure maggot variety, strong stress resistance, high activity and stable breeding are achieved.

The invention belongs to the technical field of biological prevention and control of agricultural pests, and particularly relates to a control method for inducing diapause of noctuid black egg bees. The method comprises the following steps: a, establishing a prodenia litura population and collecting new spawning blocks; b, continuously breeding the noctuid black-egg bees by taking the fresh prodenia litura eggs in the step a as hosts, and collecting female noctuid black-egg bees which are just subjected to eclosion; c, the prodenia litura eggs in the step a are inoculated with the noctuid black egg bee female bees in the step b for parasitism; and d, transferring to a diapause induction environment for treatment after parasitism. Under the conditions that the temperature is 8 DEG C and the photoperiod is 0L: 24D, or under the conditions that the temperature is 12 DEG C and the photoperiod is 8L: 16D, the diapause rate after 35-day induction treatment is relatively high, and the diapause rate reaches 97.5% or above. According to the method disclosed by the invention, the diapause rate of the black egg noctuid is remarkably improved through coordinated regulation and control of the temperature, the photoperiod and the induction time, and a key technical support is provided for long-term storage and large-scale application of the black egg noctuid.

The invention relates to the technical field of microorganisms, and discloses Pseudomonas aeruginosa HNHNPg-255 and application of the Pseudomonas aeruginosa HNHNPg-255. The strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66853. And the fermented supernatant can prevent and treat the Hami melon root knot nematode disease caused by the elephant trunk bean root knot nematode. Experiments show that the fermented supernate has a strong poisoning effect on second-stage larvae of the root-knot nematode and can remarkably inhibit incubation of egg masses and egg granules. In addition, the fermented supernate has good thermal stability and storage stability, and still keeps more than 80% of nematicidal activity after being stored for a long time at the high temperature of 121 DEG C and the temperature of 4 DEG C. Meanwhile, fermentation supernate of the strain can promote growth of Hami melon plants and improve physiological indexes such as root length, stem length, fresh weight and dry weight, and the strain has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.

The invention discloses a lepidoptera insect egg granule separation method. The method comprises the following steps: placing lepidoptera insect egg masses to be separated in a sodium bicarbonate solution with the concentration of 0.2-0.5%; the container containing the egg masses and the solution is subjected to mechanical oscillation, the oscillation rotating speed ranges from 1100 rpm to 1200 rpm, and the oscillation time ranges from 6 min to 8 min; after oscillation is finished, the treated egg granules are cleaned and dried, and the separated egg granules are obtained. A chemical reagent NaHCO3 is used as a core, mechanical vibration of an oscillator is used as an auxiliary to form a synergistic effect, efficient separation of egg masses is achieved, compared with traditional pure manual operation, the egg mass separation rate is increased to 80% from less than 30%, the egg counting error is accurately reduced to + / -3% from + / -15%, and the technology provides a standardized basis for accurate egg source supply of natural enemy breeding.

This utility model relates to a black soldier fly adult breeding device, comprising: a breeding box, a pull-out component, and a mesh cover. The breeding box has a breeding cavity with a pull-out opening on its side wall. The pull-out component passes through the opening and can be inserted into the breeding cavity. An oviposition component is mounted on the pull-out component. This allows the oviposition component to be moved through the opening to the outside of the breeding cavity, facilitating timely collection and transfer of black soldier fly egg masses on the oviposition component. This ensures the black soldier fly egg masses are in a suitable incubation environment, thereby improving the hatching effect.