The invention belongs to the technical field of vegetative propagation of seedlings, and particularly relates to a
tissue culture subculture rooting and
seedling method for
baeckea frutescens. The method comprises the following steps: inoculating subculture
bottle seedlings of
baeckea frutescens into a strong
seedling culture medium for culturing to obtain strong seedlings, wherein the formula of the strong
seedling culture medium comprises MS, 20-35g / L of
sucrose and 5-6g / L of
agar; the strong seedlings are inoculated into a rooting culture medium to be cultured to obtain rooted seedlings, and the formula of the rooting culture medium is as follows: 1 / 2MS, 1-3mg / L of IBA, 1-2mg / L of NAA and 25-35g / L of
sucrose; the rooted seedlings are transplanted outdoors for seedling hardening,
bottle seedlings are planted into a
mixed matrix of
nutrient soil and yellow
subsoil to be cultured, and water is sprayed for moisturizing. After the subculture
bottle seedlings of the
baeckea frutescens are cultured for 30 days through the strong seedling culture medium, the stems of the
baeckea frutescens are increased and thickened, the rooting rate of the obtained strong seedlings reaches 80%-100% after the strong seedlings are cultured for 60 days through the rooting culture medium, the
stem length of the
tissue culture seedlings is 5 cm or above, the number of adventitious roots is 6 or above, and the average
root length is 1.5 cm or above. After outdoor seedling hardening, the
transplanting survival rate reaches 90.15% after 30 days of culture.