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Method of detecting growth promoting effect of plant rhizosphere growth promoting bacteria

A technology of rhizosphere growth-promoting bacteria and growth-promoting bacteria, which is applied in the direction of biochemical equipment and methods, and microbial measurement/inspection. The effect of reducing material cost and improving screening efficiency

Active Publication Date: 2011-03-09
ZHANGJIAKOU GENLIDUO ECOLOGICAL AGRI TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Methods (1) and (5) are two commonly used detection methods now, but the roots in method (1) cannot elongate upright in the petri dish, which affects the measurement and observation of the root system
The stem and root of the plant can be completely taken by method (5), which is convenient for observation and measurement; but the operation of taking the root is troublesome, and watering is required during the period, which will affect the colonization of the fungus on the root of the plant
If (6) hydroponics is adopted, the bacteria are not easy to colonize on the root system of the plant, and the relationship between the bacteria and the plant cannot be explained, and the cost is relatively high

Method used

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  • Method of detecting growth promoting effect of plant rhizosphere growth promoting bacteria
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  • Method of detecting growth promoting effect of plant rhizosphere growth promoting bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 material and equipment

[0029] Plants to be tested: mung bean (Green Pearl, purchased from Shanxi Boda Seedling Co., Ltd.); corn (Nongda 108), which is commercially available.

[0030] Tested strains: CNA9 (Arthrobacter globiformis), CGMCC No.4217, MOB13 (Pseudomonas pseudoalcaligenes), CGMCC No.2763.

[0031] Test agent: 70% alcohol; Solution I: 10mM phosphoric acid; Solution II: 1mL0.5M FeCl 3 Dissolve in 50mL 35% HClO 4

[0032] Test medium: LB liquid medium: peptone 10.0g / L, yeast extract 5.0g / L, NaCl 10.0g / L, (agar 15.0g / L),

[0033] Distilled water 1000mL, pH 7.0-7.2. Autoclave at 121°C for 30min

[0034] Test materials: filter paper, large test tubes, test tube racks, artificial climate chamber, portable pressure steam sterilizer, biochemical incubator, electronic balance, digital camera, ultra-clean bench, centrifuge, ultraviolet and visible light spectrophotometer.

Embodiment 2

[0036] Select healthy mung bean seeds, immerse them in 70% alcohol for 30 seconds, rinse them with sterile water three times, wrap the seeds with two layers of gauze, put them in a large petri dish, soak them in clean water, and form a layer of water film on the surface of the gauze. Put it into a 28°C incubator and cultivate for about 18 hours until the seeds are white. After the CNA9 strain plate was streaked and activated, it was inoculated into LB medium and shaken for 3 days, and the concentration of the bacterial solution was adjusted to 10 7 CFU / mL.

[0037] The germinated seeds were treated in the following three ways: seed soaking, paper soaking, seed soaking+paper soaking, and water treatment as a control.

[0038]1) Select a number of mung bean seeds with the same degree of whiteness, and soak them in the bacterial solution for 3 hours. Roll a 20cm×30cm double-layer filter paper into a cylinder, insert it into a large test tube containing the bacteria solution, an...

Embodiment 3

[0047] Dilute the CNA9 bacterial solution after shake culture for 3 days to 10 10 , 10 9 , 10 8 , 10 7 , 10 6 , 10 5 , 10 4 , 10 3 , 10 2 , 10 1 CFU / mL, a total of 10 concentration gradients, and set water as a blank control. The mung bean seeds that germinated were inoculated with bacterial solutions of different concentrations in the manner of seed soaking and paper soaking (method is the same as in Example 2). Put them into an artificial climate box and culture them in the dark for 4 days (26°C, relative humidity: 80%). After harvesting, stem height and root length were measured. The result is as figure 2 and shown in Table 2.

[0048] The results showed that in the high-concentration bacterial solution, the stem length and root length of mung bean plants all showed inhibitory effect, while 10 7 ~10 8 The CFU / mL concentration of the bacterial solution has the most significant effect on promoting the stem length and root length of mung bean, and the optimum in...

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Abstract

The invention provides a method of detecting the growth promoting effect of plant rhizosphere growth promoting bacteria, which comprises the following steps:(1) solution of plant rhizosphere growth promoting bacteria is prepared and seeds after germination acceleration are soaked in the above solution for 2-4 hours;(2) double filter papers are soaked in the above solution and the seeds are placed at the center of the double filter papers; the double filter papers are then wrapped into a cylinder and placed in a test tube containing nutrient solution; (3) stem length, root length, dry and fresh weights of the seeds are measured after the double filter papers is placed in a climate incubator for 3-4 days. The above method is applicable to the rapid screening of plant rhizosphere growth promoting bacteria and rapid measurement of promoting effect of plant growth promoting materials. The screening time of plant rhizosphere growth promoting bacteria is reduced from one month when the seeds are cultivated in a greenhouse to one week in the present invention. The above method is of great importance to the research and industry development of microorganism fertilizers.

Description

technical field [0001] The invention relates to a method for detecting the growth-promoting effect of plant rhizosphere growth-promoting bacteria. Background technique [0002] Plant growth-promoting Rhizobacteria (PGPR) generally refers to all bacteria that can promote plant growth that exist in the plant rhizosphere, leaf surround or other habitats closely related to plants. PGPR bacteria mainly change the micro-ecological environment of soil and plants through the interaction with plant roots, so as to achieve the purpose of promoting plant growth. The mechanism of action is mainly divided into the following three types: one is to directly provide plant hormones to plants Bio-promoting substances (such as IAA, siderophilic elements, phosphorus-dissolving substances) and nitrogen fixation can enhance the absorption and utilization of nutrients by plants; the second is to achieve the effect of antibacterial by seizing ecological sites with rhizosphere pathogens and competin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 郭岩彬吴文良位婉孟凡乔周华宁焦子伟
Owner ZHANGJIAKOU GENLIDUO ECOLOGICAL AGRI TECH CO LTD
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