4 results about "Primula" patented technology

The invention belongs to the technical field of plant tissue culture and rapid propagation, and particularly provides a tissue culture and rapid propagation method for leaves and flowers of wild primulina rosea, which comprises the following steps: taking vigorously growing tender leaves at the top of primulina rosea and just blooming flowers at the top of primulina rosea as explants; pretreating an explant; disinfecting the explants; performing primary culture; carrying out subculture; rooting and seedling strengthening culture; when the strong seedling culture seedlings reach the growth indexes that the plant height is 1.8-2 cm, the root length is 1.9-2.1 cm and the leaf number is 7-8, seedling hardening treatment is conducted on the strong seedling culture seedlings; and after the seedling hardening is finished, transplanting and culturing. According to the method disclosed by the invention, the aseptic seedlings of the primulina are successfully cultivated through the tissues of the flower organs for the first time, and the transplanting success rate reaches 95% or above.

The invention relates to the technical field of plant tissue culture, in particular to a primula tibetica tissue culture rapid propagation method and application. The method comprises the following steps: drying newly harvested primula tibetica seeds in the shade, sequentially treating the seeds with 75% alcohol and 2% sodium hypochlorite, inoculating the seeds into a seed germination culture medium for culturing, shearing terminal bud parts of plants as explants, inoculating the explants into a cluster bud induction culture medium for culturing for 21-35 days, shearing the induced cluster buds into single buds, inoculating the single buds into a subculture multiplication culture medium for culturing for 45-55 days, and carrying out subculture on the single buds to obtain primula tibetica var. Tibetica var. Tibetica var. Tibetica var. Tibetica var. Tibetica var. Tibetica var. And cutting the multiple shoots subjected to subculture multiplication into small sections with 1-2 buds, inoculating the small sections into a rooting culture medium according to polarity, and culturing for 20-30 days. According to the method disclosed by the invention, the average multiplication coefficient of primary bud induction is 4.4-4.6, the rooting rate is 83.33-93.33%, and the average number of adventitious roots is more than 10.

The invention discloses a fertilizer for davidia involucrata seedlings and a fertilization method, and relates to the technical field of planting of davidia involucrata seedlings, the fertilizer for davidia involucrata seedlings comprises a nitrogen source, a phosphorus source and a potassium source, the nitrogen source is urea, the phosphorus source is calcium superphosphate, and the potassium source is potassium chloride; the fertilizing method of the fertilizer for the davidia involucrata seedlings comprises the following steps that S1, yellow brown soil and perlite are mixed according to the volume ratio of 7: 3 to form a culture medium, then rooting powder and carbendazim are sprayed into the culture medium, the culture medium is evenly stirred and then aired and disinfected, and a disinfected medium is obtained; s2, annual seedlings are selected and planted in two-color pots, each pot is filled with 2.5 kg of disinfection matrix, and then davidia involucrata seedlings are planted on the disinfection matrix; s3, after planting, applying a fertilizer to the roots of the seedlings in a water and fertilizer form during the growth period of the seedlings; s4, after fertilization, unified management and protection are kept, environmental errors are avoided, seedlings are harvested after fertilization is stopped at the end of July, and blind fertilization can be effectively avoided.

This invention discloses a genetic transformation method for Primula species, comprising the following steps: obliquely cutting the petiole of a Primula species, then immersing the petiole with leaves in an Agrobacterium rhizogenes K599 infection solution containing a recombinant vector carrying the target gene; covering the cut end of the petiole with moist substrate for cultivation; sterilizing the petiole and substrate after adventitious buds have emerged from the cut end; separating the seedlings from the petiole when the adventitious buds reach a height of 2-3 cm, and transplanting them into a mixed substrate for cultivation until complete plants are obtained. This method eliminates the need for tissue culture, is simple to operate, fills the gap in non-tissue culture transgenic methods for Primula, and boasts high transformation efficiency, short cycle, and direct screening of genetically transformed plants. It provides a simple and efficient method for studying the biological characteristics and genetic engineering breeding of this genus, possessing significant scientific research and application value.